##################################
# NO CUT ENZYMES (  207 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        AlwI        AocI        Aor51HI     
ApaI        ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiWI       BsmBI       BsmFI       
BsmI        BsoBI       BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrI        
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstF5I      BstPI       BstUI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DdeI        DpnII       DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FokI        
FseI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        HpaII       
HphI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      SbfI        ScaI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tru9I       Tsp509I     Tth111I     TthHB8I     
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   24 enzymes) #
###################################

AciI          390  CCGC                 CCGC(-3/-1)
Alw44I        287  GTGCAC               G^TGCAC
ApaLI         287  GTGCAC               G^TGCAC
AspHI         287  GTGCAC               G[AT]GC[AT]^C
BmyI          287  GTGCAC               G[AGT]GC[ACT]^C
BsaI          406  GAGACC               GGTCTC(1/5)
BsiHKAI       287  GTGCAC               G[AT]GC[AT]^C
BsmAI         406  GAGAC                GTCTC(1/5)
Bsp1286I      287  GTGCAC               G[AGT]GC[ACT]^C
Bsp1407I      449  TGTACA               T^GTACA
BsrGI         449  TGTACA               T^GTACA
BssKI         340  CCTGG                ^CC[ACGT]GG
BstNI         340  CCTGG                CC^[AT]GG
BstXI         354  CCAGAGCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoRII        340  CCTGG                ^CC[AT]GG
MboII         305  GAAGA                GAAGA(8/7)
MnlI          104  CCTC                 CCTC(7/6)
MvaI          340  CCTGG                CC^[AT]GG
NlaIII        251  CATG                 CATG^
NspI          250  ACATGC               [GA]CATG^[CT]
PflMI          47  CCACCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScrFI         340  CCTGG                CC^[ACGT]GG
SspBI         449  TGTACA               T^GTACA
Van91I         47  CCACCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

AfaI          297  GTAC                 GT^AC
              450  GTAC                
BstAPI         86  GCACACCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              230  GCACACCCTGC         
MaeIII         70  GTGAC                ^GT[ACGT]AC
              199  GTGAC               
NlaIV         259  GGGTCC               GG[ACGT]^[ACGT]CC
              364  GGCTCC              
RsaI          297  GTAC                 GT^AC
              450  GTAC                
Tsp45I         70  GTGAC                ^GT[GC]AC
              199  GTGAC               
TspRI           7  CACTG                CA[GC]TG[ACGT][ACGT]^
               66  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   20 enzymes) #
#########################################

AluI        AlwNI       AvaII       BbvI        BsiYI       
BslI        BspMI       Cac8I       Cfr13I      EcoNI       
Fnu4HI      ItaI        MslI        MspA1I      MwoI        
PstI        PvuII       Sau96I      SfcI        TseI