Restriction map for
pFN21AE6503
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BpmI BpuAI BsaBI
BsaHI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MaeIII MamI MfeI MluI MseI
MspA1I MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI Tru9I Tsp45I
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 41 CGCG CG^CG
AccIII 434 TCCGGA T^CCGGA
AciI 42 GCGG CCGC(-3/-1)
AlwI 363 GATCC GGATC(4/5)
AspHI 373 GTGCTC G[AT]GC[AT]^C
BalI 83 TGGCCA TGG^CCA
BcnI 222 CCCGG CC^[GC]GG
BlpI 111 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 111 GCTGAGC GC^T[ACGT]AGC
BsaAI 76 TACGTG [CT]AC^GT[GA]
BsaWI 434 TCCGGA [AT]^CCGG[AT]
BseAI 434 TCCGGA T^CCGGA
BseRI 94 CTCCTC GAGGAG(10/8)
BsgI 190 CTGCAC GTGCAG(16/14)
BsiHKAI 373 GTGCTC G[AT]GC[AT]^C
BspEI 434 TCCGGA T^CCGGA
BstF5I 117 CATCC GGATG(2/0)
BstNI 228 CCAGG CC^[AT]GG
BstUI 41 CGCG CG^CG
BstYI 362 AGATCC [GA]^GATC[CT]
CelII 111 GCTGAGC GC^T[ACGT]AGC
CfoI 16 GCGC GCG^C
DpnII 363 GATC ^GATC
EaeI 83 TGGCCA [CT]^GGCC[GA]
EcoRII 228 CCAGG ^CC[AT]GG
FauI 42 GCGGG CCCGC(4/6)
FokI 117 CATCC GGATG(9/13)
HhaI 16 GCGC GCG^C
HinP1I 16 GCGC G^CGC
MaeII 77 ACGT A^CGT
MboI 363 GATC ^GATC
MboII 185 TCTTC GAAGA(8/7)
MflI 362 AGATCC [GA]^GATC[CT]
MluNI 83 TGGCCA TGG^CCA
MroI 434 TCCGGA T^CCGGA
MscI 83 TGGCCA TGG^CCA
MvaI 228 CCAGG CC^[AT]GG
MvnI 41 CGCG CG^CG
MwoI 414 GCTGCCCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 222 CCCGG CC^[GC]GG
NdeII 363 GATC ^GATC
NspI 176 GCATGT [GA]CATG^[CT]
PleI 431 GAGTC GAGTC(4/5)
Sau3AI 363 GATC ^GATC
TfiI 446 GAATC G^A[AT]TC
TspRI 253 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 362 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BmyI 9 GGGCAC G[AGT]GC[ACT]^C
373 GTGCTC
BsaI 217 GAGACC GGTCTC(1/5)
91 GGTCTC
BsmAI 217 GAGAC GTCTC(1/5)
92 GTCTC
Bsp1286I 9 GGGCAC G[AGT]GC[ACT]^C
373 GTGCTC
BsrI 254 ACTGG ACTGG(1/-1)
279 CCAGT
BssKI 222 CCCGG ^CC[ACGT]GG
228 CCAGG
Cac8I 81 GCTGGC GC[ACGT]^[ACGT]GC
398 GCGTGC
DdeI 112 CTGAG C^T[ACGT]AG
350 CTAAG
DraIII 100 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
193 CACACGGTG
DsaI 57 CCGTGG C^C[GA][CT]GG
426 CCACGG
HaeIII 84 GGCC GG^CC
288 GGCC
HapII 223 CCGG C^CGG
435 CCGG
HinfI 431 GAGTC G^A[ACGT]TC
446 GAATC
HpaII 223 CCGG C^CGG
435 CCGG
MslI 39 CACGCGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
171 CATGGGCATG
MspI 223 CCGG C^CGG
435 CCGG
NlaIII 171 CATG CATG^
177 CATG
NlaIV 146 GGACCC GG[ACGT]^[ACGT]CC
275 GGGTCC
ScrFI 222 CCCGG CC^[ACGT]GG
228 CCAGG
SfaNI 62 GCATC GCATC(5/9)
116 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AfaI AluI AvaII BbvI BsaJI
BsiYI BslI BsmFI Cfr13I Fnu4HI
HphI ItaI MnlI RsaI Sau96I
TseI