################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI Alw44I AlwI AlwNI AocI ApaLI AscI AseI AsnI Asp700I Asp718I AviII AvrII BamHI BbrPI BbvCI BcgI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DrdI EagI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRI FseI FspI HindIII HpaI KpnI KspI MaeI MaeII MfeI MflI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AccII 1372 CGCG CG^CG AcsI 817 AAATTC [GA]^AATT[CT] AcyI 946 GGCGCC G[GA]^CG[CT]C AhdI 308 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 665 AGCGCT AGC^GCT ApaI 1229 GGGCCC GGGCC^C ApoI 817 AAATTC [GA]^AATT[CT] AspEI 308 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1309 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1146 TGGCCA TGG^CCA BbeI 946 GGCGCC GGCGC^C BbsI 151 GAAGAC GAAGAC(2/6) BciVI 1023 GTATCC GTATCC(6/5) BclI 1322 TGATCA T^GATCA BpuAI 151 GAAGAC GAAGAC(2/6) BsaBI 1348 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 946 GGCGCC G[GA]^CG[CT]C BsmBI 1487 CGTCTC CGTCTC(1/5) BsmFI 701 GGGAC GGGAC(10/14) BspHI 102 TCATGA T^CATGA BsrBI 1266 CCGCTC CCGCTC(-3/-3) BstEII 475 GGTAACC G^GT[ACGT]ACC BstPI 475 GGTAACC G^GT[ACGT]ACC BstUI 1372 CGCG CG^CG DraI 213 TTTAAA TTT^AAA EaeI 1146 TGGCCA [CT]^GGCC[GA] Eam1105I 308 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 665 AGCGCT AGC^GCT EcoO65I 475 GGTAACC G^GT[ACGT]ACC EcoRV 1457 GATATC GAT^ATC EcoT22I 542 ATGCAT ATGCA^T FbaI 1322 TGATCA T^GATCA HapII 1041 CCGG C^CGG HgaI 1373 GCGTC GACGC(5/10) Hin1I 946 GGCGCC G[GA]^CG[CT]C HincII 850 GTCAAC GT[CT]^[GA]AC HindII 850 GTCAAC GT[CT]^[GA]AC HpaII 1041 CCGG C^CGG KasI 946 GGCGCC G^GCGCC MamI 1348 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1146 TGGCCA TGG^CCA MscI 1146 TGGCCA TGG^CCA MspA1I 68 CAGCGG C[AC]G^C[GT]G MspI 1041 CCGG C^CGG MvnI 1372 CGCG CG^CG NarI 946 GGCGCC GG^CGCC NcoI 227 CCATGG C^CATGG NsiI 542 ATGCAT ATGCA^T PflFI 1309 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 337 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 102 TCATGA T^CATGA SfcI 84 CTACAG C^T[GA][CT]AG SfoI 946 GGCGCC GGC^GCC SmaI 1040 CCCGGG CCC^GGG SphI 544 GCATGC GCATG^C StuI 1125 AGGCCT AGG^CCT Tth111I 1309 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 337 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 989 CCACCTCGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1040 CCCGGG C^CCGGG XmaI 1040 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AfaI 674 GTAC GT^AC 763 GTAC AvaI 1040 CCCGGG C^[CT]CG[GA]G 1226 CTCGGG BanI 946 GGCGCC G^G[CT][GA]CC 1060 GGTGCC BcnI 1040 CCCGG CC^[GC]GG 1041 CCGGG BsgI 499 CTGCAC GTGCAG(16/14) 65 GTGCAG BsoBI 1040 CCCGGG C^[CT]CG[GA]G 1226 CTCGGG BsrDI 782 GCAATG GCAATG(2/0) 1235 GCAATG BstAPI 45 GCAGTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 58 GCAGAAAGTGC BstNI 697 CCAGG CC^[AT]GG 1383 CCTGG DpnII 1323 GATC ^GATC 1471 GATC EcoRII 697 CCAGG ^CC[AT]GG 1383 CCTGG EcoT14I 227 CCATGG C^C[AT][AT]GG 965 CCTTGG MboI 1323 GATC ^GATC 1471 GATC MslI 418 CATACTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 434 CATTCCAGTG MvaI 697 CCAGG CC^[AT]GG 1383 CCTGG NciI 1040 CCCGG CC^[GC]GG 1041 CCGGG NdeII 1323 GATC ^GATC 1471 GATC PleI 1153 GACTC GAGTC(4/5) 1309 GACTC RsaI 674 GTAC GT^AC 763 GTAC SacI 755 GAGCTC GAGCT^C 889 GAGCTC SapI 241 GAAGAGC GCTCTTC(1/4) 1104 GAAGAGC Sau3AI 1323 GATC ^GATC 1471 GATC StyI 227 CCATGG C^C[AT][AT]GG 965 CCTTGG Tsp509I 818 AATT ^AATT 859 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AflIII AluI AspHI AvaII BanII BbvI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspLU11I BsrI BssKI BstF5I Cac8I CfoI Cfr13I DdeI DsaI EarI FauI Fnu4HI FokI HaeII HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeIII MboII MnlI MseI MwoI NlaIII NlaIV NspI Sau96I ScrFI SfaNI SmlI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I