(Circular) (Six-base) MAPSORT of: pFN21AB5945.seq Check: 1770 from: 1 to: 6232 pFN21AB5945 6232 bp With 182 enzymes: SgfI * March 28, 2012 14:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4042 278 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3575 1937 Size: 1638 4594 Acc65I G'GTAC_C Cuts at: 3553 3553 Size: 6232 AclI AA'CG_TT Cuts at: 1563 4359 4732 1563 Size: 2796 373 3063 Fragments arranged by size: 3063 2796 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2672 3278 3548 4295 5679 5947 1632 Size: 1040 606 270 747 1384 268 1917 Fragments arranged by size: 1917 1384 1040 747 606 270 268 AfeI AGC'GCT Cuts at: 2672 2672 Size: 6232 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6035 AflIII A'CryG_T Cuts at: 1203 2314 2874 3453 5131 6108 1203 Size: 1111 560 579 1678 977 1327 Fragments arranged by size: 1678 1327 1111 977 579 560 AgeI A'CCGG_T Cuts at: 5063 5063 Size: 6232 AhdI GACnn_n'nnGTC Cuts at: 2318 4961 2318 Size: 2643 3589 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6232 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3263 1184 Size: 2079 4153 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2424 2456 2424 Size: 32 6200 AlwNI CAG_nnn'CTG Cuts at: 3975 5547 3975 Size: 1572 4660 ApaI G_GGCC'C Cuts at: 3238 3238 Size: 6232 ApaLI G'TGCA_C Cuts at: 4289 5445 4289 Size: 1156 5076 ApoI r'AATT_y Cuts at: 1493 2822 3541 3767 3919 6028 1493 Size: 1329 719 226 152 2109 1697 Fragments arranged by size: 2109 1697 1329 719 226 152 AseI AT'TA_AT Cuts at: 160 1032 4786 160 Size: 872 3754 1606 Fragments arranged by size: 3754 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 AvaI C'yCGr_G Cuts at: 1870 1943 3045 3231 3557 1870 Size: 73 1102 186 326 4545 Fragments arranged by size: 4545 1102 326 186 73 BamHI G'GATC_C Cuts at: 3562 3929 3562 Size: 367 5865 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2951 3065 3553 5008 618 Size: 325 217 627 1164 114 488 1455 1842 Fragments arranged by size: 1842 1455 1164 627 488 325 217 114 BanII G_rGCy'C Cuts at: 1391 2764 2898 3238 3523 3551 1391 Size: 1373 134 340 285 28 4072 Fragments arranged by size: 4072 1373 340 285 134 28 BbeI G_GCGC'C Cuts at: 2955 2955 Size: 6232 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2163 928 Size: 971 264 4997 Fragments arranged by size: 4997 971 264 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4423 4457 1903 Size: 34 2486 34 3678 Fragments arranged by size: 3678 2486 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3039 4124 5334 3039 Size: 1085 1210 3937 Fragments arranged by size: 3937 1210 1085 BclI T'GATC_A Cuts at: 1548 3327 1548 Size: 1779 4453 BfrBI ATG'CAT Cuts at: 2549 2549 Size: 6232 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4843 136 Size: 107 122 71 631 605 3171 1525 Fragments arranged by size: 3171 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6227 6227 Size: 6232 BlpI GC'TnA_GC Cuts at: 3649 3649 Size: 6232 Bme1580I G_kGCm'C Cuts at: 1790 2061 3070 3238 4293 5449 1790 Size: 271 1009 168 1055 1156 2573 Fragments arranged by size: 2573 1156 1055 1009 271 168 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6232 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3963 4921 442 Size: 416 53 83 399 24 2546 958 1753 Fragments arranged by size: 2546 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6232 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2585 2585 Size: 6232 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4892 1239 Size: 3653 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2675 3057 3272 4357 5222 5520 2675 Size: 382 215 1085 865 298 3387 Fragments arranged by size: 3387 1085 865 382 298 215 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4895 882 Size: 617 3396 2219 Fragments arranged by size: 3396 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4023 493 Size: 711 2819 2702 Fragments arranged by size: 2819 2702 711 BsaBI GATnn'nnATC Cuts at: 3357 6226 3357 Size: 2869 3363 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2952 4039 4421 5056 Size: 53 83 186 936 1419 1087 382 635 Cuts at: 5056 275 Size: 1451 Fragments arranged by size: 1451 1419 1087 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4665 5063 5337 5484 5895 4665 Size: 398 274 147 411 5002 Fragments arranged by size: 5002 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6007 Fragments arranged by size: 6007 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2477 2672 3275 3522 1186 Size: 1291 195 603 247 3896 Fragments arranged by size: 3896 1291 603 247 195 BseYI C'CCAG_C Cuts at: 2140 5435 5931 2140 Size: 3295 496 2441 Fragments arranged by size: 3295 2441 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2091 2489 1163 Size: 573 355 398 4906 Fragments arranged by size: 4906 573 398 355 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3682 4443 5471 1540 Size: 275 186 1681 761 1028 2301 Fragments arranged by size: 2301 1681 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2664 2696 2764 2898 4293 4378 5449 2664 Size: 32 68 134 1395 85 1071 3447 Fragments arranged by size: 3447 1395 1071 134 85 68 32 BsmI GAATG_Cn' Cuts at: 3743 3836 3743 Size: 93 6139 BsmBI CGTCTCn'nnnn_ Cuts at: 3498 3498 Size: 6232 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2664 2696 2764 2898 3070 3238 Size: 399 271 603 32 68 134 172 168 Cuts at: 3238 3523 3551 4293 4378 5449 1391 Size: 285 28 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 603 399 285 271 172 168 134 85 68 32 28 BspEI T'CCGG_A Cuts at: 5895 5895 Size: 6232 BspHI T'CATG_A Cuts at: 2107 4121 5851 2107 Size: 2014 1730 2488 Fragments arranged by size: 2488 2014 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3587 844 Size: 2743 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3273 4119 1394 Size: 429 1450 846 3507 Fragments arranged by size: 3507 1450 846 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2794 3247 4727 4901 65 Size: 1183 1546 453 1480 174 1396 Fragments arranged by size: 1546 1480 1396 1183 453 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4876 5063 1700 Size: 110 3066 187 2869 Fragments arranged by size: 3066 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6232 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6232 BssSI C'ACGA_G Cuts at: 4292 5304 4292 Size: 1012 5220 BstAPI GCAn_nnn'nTGC Cuts at: 794 2056 2069 794 Size: 1262 13 4957 Fragments arranged by size: 4957 1262 13 BstBI TT'CG_AA Cuts at: 5036 5036 Size: 6232 BstEII G'GTnAC_C Cuts at: 1184 2480 1184 Size: 1296 4936 BstYI r'GATC_y Cuts at: 1972 3562 3929 4314 4331 5783 5869 5881 6227 Size: 1590 367 385 17 1452 86 12 346 Cuts at: 6227 1972 Size: 1977 Fragments arranged by size: 1977 1590 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2232 3162 3424 513 Size: 552 939 228 930 262 3321 Fragments arranged by size: 3321 939 930 552 262 228 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2379 487 Size: 683 56 1153 4340 Fragments arranged by size: 4340 1153 683 56 BtsI GCAGTG_nn' Cuts at: 2709 2842 3370 3748 4542 4562 2709 Size: 133 528 378 794 20 4379 Fragments arranged by size: 4379 794 528 378 133 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6232 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2772 2807 3130 3165 529 Size: 35 2208 35 323 35 3596 Fragments arranged by size: 3596 2208 323 35 35 35 DraI TTT'AAA Cuts at: 2220 3536 2220 Size: 1316 4916 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5239 809 Size: 507 3923 1802 Fragments arranged by size: 3923 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3151 3679 4568 Size: 54 1425 50 275 18 1321 528 889 Cuts at: 4568 6197 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1321 889 528 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3679 1537 Size: 275 1867 4090 Fragments arranged by size: 4090 1867 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2241 2554 3104 3389 4162 1353 Size: 888 313 550 285 773 3423 Fragments arranged by size: 3423 888 773 550 313 285 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2480 4819 5193 5339 387 Size: 1064 138 891 2339 374 146 1280 Fragments arranged by size: 2339 1280 1064 891 374 146 138 EcoICRI GAG'CTC Cuts at: 2762 2896 2762 Size: 134 6098 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2672 3278 3548 4295 4892 5679 5947 Size: 393 1040 606 270 747 597 787 268 Cuts at: 5947 1239 Size: 1524 Fragments arranged by size: 1524 1040 787 747 606 597 393 270 268 EcoNI CCTnn'n_nnAGG Cuts at: 3527 3527 Size: 6232 EcoRI G'AATT_C Cuts at: 3541 3919 3541 Size: 378 5854 EcoRV GAT'ATC Cuts at: 3464 3464 Size: 6232 FspI TGC'GCA Cuts at: 4738 4738 Size: 6232 HaeII r_GCGC'y Cuts at: 1678 2674 2842 2955 5379 1678 Size: 996 168 113 2424 2531 Fragments arranged by size: 2531 2424 996 168 113 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2304 2336 3303 3335 3476 Size: 32 135 32 732 32 967 32 141 Cuts at: 3476 3508 4873 4905 4947 4979 1373 Size: 32 1365 32 42 32 2626 Fragments arranged by size: 2626 1365 967 732 141 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2857 3576 3822 669 Size: 988 281 919 719 246 3079 Fragments arranged by size: 3079 988 919 719 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6232 HpaI GTT'AAC Cuts at: 3822 3822 Size: 6232 KasI G'GCGC_C Cuts at: 2951 2951 Size: 6232 KpnI G_GTAC'C Cuts at: 3557 3557 Size: 6232 MfeI C'AATT_G Cuts at: 3831 3831 Size: 6232 MluI A'CGCG_T Cuts at: 6108 6108 Size: 6232 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2286 5346 5530 1305 Size: 981 3060 184 2007 Fragments arranged by size: 3060 2007 981 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3153 10 Size: 54 1425 343 1321 3089 Fragments arranged by size: 3089 1425 1321 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2427 2443 4190 4549 4708 518 Size: 648 1261 16 1747 359 159 2042 Fragments arranged by size: 2042 1747 1261 648 359 159 16 MspA1I CmG'CkG Cuts at: 1606 2075 3648 4325 5473 5718 1606 Size: 469 1573 677 1148 245 2120 Fragments arranged by size: 2120 1573 1148 677 469 245 NarI GG'CG_CC Cuts at: 2952 2952 Size: 6232 NcoI C'CATG_G Cuts at: 513 1065 2004 2232 513 Size: 552 939 228 4513 Fragments arranged by size: 4513 939 552 228 NdeI CA'TA_TG Cuts at: 387 387 Size: 6232 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6232 NotI GC'GGCC_GC Cuts at: 3679 3679 Size: 6232 NsiI A_TGCA'T Cuts at: 2551 2551 Size: 6232 NspI r_CATG'y Cuts at: 1132 1229 2318 2553 2878 3028 3457 3590 5135 Size: 97 1089 235 325 150 429 133 1545 Cuts at: 5135 1132 Size: 2229 Fragments arranged by size: 2229 1545 1089 429 325 235 150 133 97 PasI CC'CwG_GG Cuts at: 1348 2702 1348 Size: 1354 4878 PciI A'CATG_T Cuts at: 2314 2874 3453 5131 2314 Size: 560 579 1678 3415 Fragments arranged by size: 3415 1678 579 560 PflMI CCAn_nnn'nTGG Cuts at: 1108 2348 1108 Size: 1240 4992 PfoI T'CCnGG_A Cuts at: 3387 3387 Size: 6232 PmeI GTTT'AAAC Cuts at: 3536 3536 Size: 6232 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4301 4333 5814 5846 4301 Size: 32 1481 32 4687 Fragments arranged by size: 4687 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6232 PsiI TTA'TAA Cuts at: 3802 3802 Size: 6232 PspOMI G'GGCC_C Cuts at: 3234 3234 Size: 6232 PstI C_TGCA'G Cuts at: 830 3584 830 Size: 2754 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6232 SacI G_AGCT'C Cuts at: 2764 2898 2764 Size: 134 6098 SalI G'TCGA_C Cuts at: 1936 3574 1936 Size: 1638 4594 SapI GCTCTTCn'nnn_ Cuts at: 2241 3104 2241 Size: 863 5369 SbfI CC_TGCA'GG Cuts at: 3584 3584 Size: 6232 ScaI AGT'ACT Cuts at: 4480 6192 4480 Size: 1712 4520 SfcI C'TryA_G Cuts at: 826 1045 2089 3580 4715 5396 5587 826 Size: 219 1044 1491 1135 681 191 1471 Fragments arranged by size: 1491 1471 1135 1044 681 219 191 SfoI GGC'GCC Cuts at: 2953 2953 Size: 6232 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6232 SmaI CCC'GGG Cuts at: 1872 3047 3559 1872 Size: 1175 512 4545 Fragments arranged by size: 4545 1175 512 SmlI C'TyrA_G Cuts at: 820 1017 1943 2654 3072 3287 4336 5237 5499 Size: 197 926 711 418 215 1049 901 262 Cuts at: 5499 820 Size: 1553 Fragments arranged by size: 1553 1049 926 901 711 418 262 215 197 SnaBI TAC'GTA Cuts at: 493 4023 493 Size: 3530 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6232 SphI G_CATG'C Cuts at: 1132 2553 3590 1132 Size: 1421 1037 3774 Fragments arranged by size: 3774 1421 1037 SspI AAT'ATT Cuts at: 5 52 4156 5 Size: 47 4104 2081 Fragments arranged by size: 4104 2081 47 StuI AGG'CCT Cuts at: 1517 3132 1517 Size: 1615 4617 StyI C'CwwG_G Cuts at: 513 1065 2004 2232 2970 3671 513 Size: 552 939 228 738 701 3074 Fragments arranged by size: 3074 939 738 701 552 228 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4259 4276 4429 4614 5059 325 Size: 1255 2679 17 153 185 445 1498 Fragments arranged by size: 2679 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4478 6190 96 Size: 274 80 33 51 1443 2501 1712 138 Fragments arranged by size: 2501 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6192 6224 6192 Size: 32 6200 Tth111I GACn'n_nGTC Cuts at: 1316 3317 1316 Size: 2001 4231 XbaI T'CTAG_A Cuts at: 3568 3568 Size: 6232 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3001 3001 Size: 6232 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6232 XmaI C'CCGG_G Cuts at: 1870 3045 3557 1870 Size: 1175 512 4545 Fragments arranged by size: 4545 1175 512 XmnI GAAnn'nnTTC Cuts at: 4361 4361 Size: 6232 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4040 276 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoO109I FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI