Restriction map for
pFN21AB5945
(Linear) (Six-base) MAPSORT of: KIBB5945.seq Check: 2383 from: 1 to: 1527
KIBB5945.orf
With 182 enzymes: SgfI *
March 22, 2012 15:33 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 1273 1527
Size: 667 606 254
Fragments arranged by size:
667 606 254
AfeI AGC'GCT
Cuts at: 0 667 1527
Size: 667 860
AflIII A'CryG_T
Cuts at: 0 309 869 1448 1527
Size: 309 560 579 79
Fragments arranged by size:
579 560 309 79
AhdI GACnn_n'nnGTC
Cuts at: 0 313 1527
Size: 313 1214
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1258 1527
Size: 1258 269
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 419 451 1527
Size: 419 32 1076
Fragments arranged by size:
1076 419 32
ApaI G_GGCC'C
Cuts at: 0 1233 1527
Size: 1233 294
ApoI r'AATT_y
Cuts at: 0 817 1527
Size: 817 710
AvaI C'yCGr_G
Cuts at: 0 1040 1226 1527
Size: 1040 186 301
Fragments arranged by size:
1040 301 186
BanI G'GyrC_C
Cuts at: 0 946 1060 1527
Size: 946 114 467
Fragments arranged by size:
946 467 114
BanII G_rGCy'C
Cuts at: 0 759 893 1233 1518 1527
Size: 759 134 340 285 9
Fragments arranged by size:
759 340 285 134 9
BbeI G_GCGC'C
Cuts at: 0 950 1527
Size: 950 577
BbsI GAAGACnn'nnnn_
Cuts at: 0 158 1527
Size: 158 1369
BciVI GTATCCnnnnn_n'
Cuts at: 0 1034 1527
Size: 1034 493
BclI T'GATC_A
Cuts at: 0 1322 1527
Size: 1322 205
BfrBI ATG'CAT
Cuts at: 0 544 1527
Size: 544 983
Bme1580I G_kGCm'C
Cuts at: 0 56 1065 1233 1527
Size: 56 1009 168 294
Fragments arranged by size:
1009 294 168 56
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 580 1527
Size: 580 947
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 1052 1267 1527
Size: 670 382 215 260
Fragments arranged by size:
670 382 260 215
BsaBI GATnn'nnATC
Cuts at: 0 1352 1527
Size: 1352 175
BsaHI Gr'CG_yC
Cuts at: 0 947 1527
Size: 947 580
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 472 667 1270 1517 1527
Size: 472 195 603 247 10
Fragments arranged by size:
603 472 247 195 10
BseYI C'CCAG_C
Cuts at: 0 135 1527
Size: 135 1392
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 484 1527
Size: 86 398 1043
Fragments arranged by size:
1043 398 86
BsiHKAI G_wGCw'C
Cuts at: 0 659 691 759 893 1527
Size: 659 32 68 134 634
Fragments arranged by size:
659 634 134 68 32
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1493 1527
Size: 1493 34
Bsp1286I G_dGCh'C
Cuts at: 0 56 659 691 759 893 1065 1233 1518
Size: 56 603 32 68 134 172 168 285
Cuts at: 1518 1527
Size: 9
Fragments arranged by size:
603 285 172 168 134 68 56 32
9
BspHI T'CATG_A
Cuts at: 0 102 1527
Size: 102 1425
BsrBI CCG'CTC
Cuts at: 0 1268 1527
Size: 1268 259
BsrDI GCAATG_nn'
Cuts at: 0 789 1242 1527
Size: 789 453 285
Fragments arranged by size:
789 453 285
BstAPI GCAn_nnn'nTGC
Cuts at: 0 51 64 1527
Size: 51 13 1463
Fragments arranged by size:
1463 51 13
BstEII G'GTnAC_C
Cuts at: 0 475 1527
Size: 475 1052
BtgI C'CryG_G
Cuts at: 0 227 1157 1419 1527
Size: 227 930 262 108
Fragments arranged by size:
930 262 227 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 374 1527
Size: 374 1153
BtsI GCAGTG_nn'
Cuts at: 0 704 837 1365 1527
Size: 704 133 528 162
Fragments arranged by size:
704 528 162 133
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 767 802 1125 1160 1527
Size: 767 35 323 35 367
Fragments arranged by size:
767 367 323 35 35
DraI TTT'AAA
Cuts at: 0 215 1527
Size: 215 1312
EaeI y'GGCC_r
Cuts at: 0 1146 1527
Size: 1146 381
EarI CTCTTCn'nnn_
Cuts at: 0 236 549 1099 1384 1527
Size: 236 313 550 285 143
Fragments arranged by size:
550 313 285 236 143
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 475 1527
Size: 475 1052
EcoICRI GAG'CTC
Cuts at: 0 757 891 1527
Size: 757 134 636
Fragments arranged by size:
757 636 134
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 1273 1527
Size: 667 606 254
Fragments arranged by size:
667 606 254
EcoRV GAT'ATC
Cuts at: 0 1459 1527
Size: 1459 68
HaeII r_GCGC'y
Cuts at: 0 669 837 950 1527
Size: 669 168 113 577
Fragments arranged by size:
669 577 168 113
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 299 331 1298 1330 1471 1503 1527
Size: 299 32 967 32 141 32 24
Fragments arranged by size:
967 299 141 32 32 32 24
HincII GTy'rAC
Cuts at: 0 852 1527
Size: 852 675
KasI G'GCGC_C
Cuts at: 0 946 1527
Size: 946 581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 1527
Size: 281 1246
MscI TGG'CCA
Cuts at: 0 1148 1527
Size: 1148 379
MslI CAynn'nnrTG
Cuts at: 0 422 438 1527
Size: 422 16 1089
Fragments arranged by size:
1089 422 16
MspA1I CmG'CkG
Cuts at: 0 70 1527
Size: 70 1457
NarI GG'CG_CC
Cuts at: 0 947 1527
Size: 947 580
NcoI C'CATG_G
Cuts at: 0 227 1527
Size: 227 1300
NsiI A_TGCA'T
Cuts at: 0 546 1527
Size: 546 981
NspI r_CATG'y
Cuts at: 0 313 548 873 1023 1452 1527
Size: 313 235 325 150 429 75
Fragments arranged by size:
429 325 313 235 150 75
PasI CC'CwG_GG
Cuts at: 0 697 1527
Size: 697 830
PciI A'CATG_T
Cuts at: 0 309 869 1448 1527
Size: 309 560 579 79
Fragments arranged by size:
579 560 309 79
PflMI CCAn_nnn'nTGG
Cuts at: 0 343 1527
Size: 343 1184
PfoI T'CCnGG_A
Cuts at: 0 1382 1527
Size: 1382 145
PspOMI G'GGCC_C
Cuts at: 0 1229 1527
Size: 1229 298
SacI G_AGCT'C
Cuts at: 0 759 893 1527
Size: 759 134 634
Fragments arranged by size:
759 634 134
SapI GCTCTTCn'nnn_
Cuts at: 0 236 1099 1527
Size: 236 863 428
Fragments arranged by size:
863 428 236
SfcI C'TryA_G
Cuts at: 0 84 1527
Size: 84 1443
SfoI GGC'GCC
Cuts at: 0 948 1527
Size: 948 579
SmaI CCC'GGG
Cuts at: 0 1042 1527
Size: 1042 485
SmlI C'TyrA_G
Cuts at: 0 649 1067 1282 1527
Size: 649 418 215 245
Fragments arranged by size:
649 418 245 215
SphI G_CATG'C
Cuts at: 0 548 1527
Size: 548 979
StuI AGG'CCT
Cuts at: 0 1127 1527
Size: 1127 400
StyI C'CwwG_G
Cuts at: 0 227 965 1527
Size: 227 738 562
Fragments arranged by size:
738 562 227
Tth111I GACn'n_nGTC
Cuts at: 0 1312 1527
Size: 1312 215
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 996 1527
Size: 996 531
XmaI C'CCGG_G
Cuts at: 0 1040 1527
Size: 1040 487
Enzymes that do cut:
AcuI AfeI AflIII AhdI AlfI AloI ApaI ApoI
AvaI BanI BanII BbeI BbsI BciVI BclI BfrBI
Bme1580I BplI BpuEI BsaBI BsaHI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspHI BsrBI BsrDI BstAPI BstEII
BtgI BtgZI BtsI CspCI DraI EaeI EarI EciI
EcoICRI Eco57MI EcoRV HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NarI NcoI NsiI NspI PasI
PciI PflMI PfoI PspOMI SacI SapI SfcI SfoI
SmaI SmlI SphI StuI StyI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AleI
AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BglI BglII BlpI BmgBI BmrI BmtI
BpmI Bpu10I BsaI BsaAI BsaWI BsaXI BsiEI BsiWI
BsmI BspEI BspMI BsrFI BsrGI BssHII BssSI BstBI
BstXI BstYI BstZ17I Bsu36I ClaI DraIII DrdI EagI
EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI
NotI NruI PacI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI TaqII TatI TstI
XbaI XhoI XmnI ZraI