Restriction map for
pFN21AE1775
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AflIII AhdI AlwI AocI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BciVI BclI BfaI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsgI BsiEI BsiWI BsmBI
BspDI BspHI BspLU11I BspMI BsrDI
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
EaeI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FokI FseI
FspI HindIII HpaI KasI Ksp632I
KspI MaeI MaeIII MamI MfeI
MluI MluNI MscI MseI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SmlI SnaBI
SpeI SphI Sse8387I SspI StyI
SwaI TfiI Tru9I Tsp45I Tsp509I
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 662 GGTACC G^GTACC
AccIII 211 TCCGGA T^CCGGA
AgeI 199 ACCGGT A^CCGGT
Alw44I 475 GTGCAC G^TGCAC
Aor51HI 83 AGCGCT AGC^GCT
ApaLI 475 GTGCAC G^TGCAC
Asp718I 662 GGTACC G^GTACC
AspHI 475 GTGCAC G[AT]GC[AT]^C
AvaI 51 CCCGGG C^[CT]CG[GA]G
BbsI 814 GTCTTC GAAGAC(2/6)
BbvCI 612 GCTGAGG CCTCAGC(-5/-2)
BcgI 218 GCACCCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 618 GCCTGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 567 AGATCT A^GATCT
BpuAI 814 GTCTTC GAAGAC(2/6)
BsaI 534 GGTCTC GGTCTC(1/5)
BseAI 211 TCCGGA T^CCGGA
BsiHKAI 475 GTGCAC G[AT]GC[AT]^C
BsmAI 535 GTCTC GTCTC(1/5)
BsmI 412 GAATGC GAATGC(1/-1)
BsoBI 51 CCCGGG C^[CT]CG[GA]G
Bsp1407I 374 TGTACA T^GTACA
BspEI 211 TCCGGA T^CCGGA
BsrBI 3 GAGCGG CCGCTC(-3/-3)
BsrFI 199 ACCGGT [GA]^CCGG[CT]
BsrGI 374 TGTACA T^GTACA
BssHII 96 GCGCGC G^CGCGC
BstYI 567 AGATCT [GA]^GATC[CT]
Cfr10I 199 ACCGGT [GA]^CCGG[CT]
DpnII 568 GATC ^GATC
DrdI 526 GACGCCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 163 CCGTGG C^C[GA][CT]GG
Eco47III 83 AGCGCT AGC^GCT
EcoNI 393 CCTCATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 313 GTCAAC GT[CT]^[GA]AC
HindII 313 GTCAAC GT[CT]^[GA]AC
KpnI 662 GGTACC GGTAC^C
MaeII 473 ACGT A^CGT
MboI 568 GATC ^GATC
MflI 567 AGATCT [GA]^GATC[CT]
MroI 211 TCCGGA T^CCGGA
NdeII 568 GATC ^GATC
PinAI 199 ACCGGT A^CCGGT
Sau3AI 568 GATC ^GATC
SgrAI 198 CACCGGTG C[GA]^CCGG[CT]G
SmaI 51 CCCGGG CCC^GGG
SspBI 374 TGTACA T^GTACA
StuI 616 AGGCCT AGG^CCT
XcmI 680 CCAGGCTGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 567 AGATCT [GA]^GATC[CT]
XmaCI 51 CCCGGG C^CCGGG
XmaI 51 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
ApaI 587 GGGCCC GGGCC^C
644 GGGCCC
BsaWI 199 ACCGGT [AT]^CCGG[AT]
211 TCCGGA
BsmFI 134 GGGAC GGGAC(10/14)
752 GGGAC
BsrI 365 ACTGG ACTGG(1/-1)
697 CCAGT
HinfI 43 GACTC G^A[ACGT]TC
655 GAGTC
MboII 815 TCTTC GAAGA(8/7)
818 TCTTC
MslI 288 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
468 CATCGACGTG
NspI 371 GCATGT [GA]CATG^[CT]
384 ACATGC
PflMI 321 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
486 CCAGCCCCTGG
PleI 43 GACTC GAGTC(4/5)
655 GAGTC
PstI 270 CTGCAG CTGCA^G
340 CTGCAG
PvuII 256 CAGCTG CAG^CTG
436 CAGCTG
SfcI 270 CTGCAG C^T[GA][CT]AG
340 CTGCAG
TspRI 264 CAGTG CA[GC]TG[ACGT][ACGT]^
698 CAGTG
Van91I 321 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
486 CCAGCCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaHI BsaJI
BseRI BsiYI BslI Bsp1286I BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DraII EcoO109I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII HphI
ItaI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
PpuMI RsaI Sau96I ScrFI TaqI
TseI TthHB8I