(Circular) (Six-base) MAPSORT of: pFN21AE1775.seq Check: 9540 from: 1 to: 5551 pFN21AE1775 5551 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3361 278 Size: 53 83 186 936 1825 2468 Fragments arranged by size: 2468 1825 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2894 1937 Size: 957 4594 Acc65I G'GTAC_C Cuts at: 2667 2872 2667 Size: 205 5346 AclI AA'CG_TT Cuts at: 1563 3678 4051 1563 Size: 2115 373 3063 Fragments arranged by size: 3063 2115 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2774 3614 4998 5266 1632 Size: 1142 840 1384 268 1917 Fragments arranged by size: 1917 1384 1142 840 268 AfeI AGC'GCT Cuts at: 2090 2090 Size: 5551 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5354 AflIII A'CryG_T Cuts at: 1203 4450 5427 1203 Size: 3247 977 1327 Fragments arranged by size: 3247 1327 977 AgeI A'CCGG_T Cuts at: 2204 4382 2204 Size: 2178 3373 AhdI GACnn_n'nnGTC Cuts at: 4280 4280 Size: 5551 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5551 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5551 AlwNI CAG_nnn'CTG Cuts at: 2045 2274 2332 2497 2708 3294 4866 2045 Size: 229 58 165 211 586 1572 2730 Fragments arranged by size: 2730 1572 586 229 211 165 58 ApaI G_GGCC'C Cuts at: 2596 2653 2596 Size: 57 5494 ApaLI G'TGCA_C Cuts at: 2480 3608 4764 2480 Size: 1128 1156 3267 Fragments arranged by size: 3267 1156 1128 ApoI r'AATT_y Cuts at: 1493 2860 3086 3238 5347 1493 Size: 1367 226 152 2109 1697 Fragments arranged by size: 2109 1697 1367 226 152 AseI AT'TA_AT Cuts at: 160 1032 4105 160 Size: 872 3073 1606 Fragments arranged by size: 3073 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5551 AvaI C'yCGr_G Cuts at: 1870 1943 2056 2876 1870 Size: 73 113 820 4545 Fragments arranged by size: 4545 820 113 73 BamHI G'GATC_C Cuts at: 2881 3248 2881 Size: 367 5184 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2631 2667 2698 2872 4327 Size: 325 217 627 844 36 31 174 1455 Cuts at: 4327 618 Size: 1842 Fragments arranged by size: 1842 1455 844 627 325 217 174 36 31 BanII G_rGCy'C Cuts at: 1391 2513 2570 2596 2653 2836 2870 1391 Size: 1122 57 26 57 183 34 4072 Fragments arranged by size: 4072 1122 183 57 57 34 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2812 928 Size: 971 913 3667 Fragments arranged by size: 3667 971 913 BbvCI CC'TCA_GC Cuts at: 2618 2618 Size: 5551 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2212 2246 3742 3776 1903 Size: 34 275 34 1496 34 3678 Fragments arranged by size: 3678 1496 275 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3443 4653 3443 Size: 1210 4341 BclI T'GATC_A Cuts at: 1548 1548 Size: 5551 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2629 4162 136 Size: 107 122 71 631 605 957 1533 1525 Fragments arranged by size: 1533 1525 957 631 605 122 107 71 BglII A'GATC_T Cuts at: 2572 5546 2572 Size: 2974 2577 BlpI GC'TnA_GC Cuts at: 2968 2968 Size: 5551 Bme1580I G_kGCm'C Cuts at: 1790 2484 2596 2634 2653 3612 4768 1790 Size: 694 112 38 19 959 1156 2573 Fragments arranged by size: 2573 1156 959 694 112 38 19 BmgBI CAC'GTC Cuts at: 1318 2479 1318 Size: 1161 4390 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3282 4240 442 Size: 416 53 83 399 24 1865 958 1753 Fragments arranged by size: 1865 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5551 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2532 2532 Size: 5551 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2333 2392 2675 4211 1239 Size: 1094 59 283 1536 2579 Fragments arranged by size: 2579 1536 1094 283 59 Bpu10I CC'TnA_GC Cuts at: 2618 2618 Size: 5551 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3676 4541 4839 3676 Size: 865 298 4388 Fragments arranged by size: 4388 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2545 4214 882 Size: 617 1046 1669 2219 Fragments arranged by size: 2219 1669 1046 617 BsaAI yAC'GTr Cuts at: 493 1204 3342 493 Size: 711 2138 2702 Fragments arranged by size: 2702 2138 711 BsaBI GATnn'nnATC Cuts at: 5545 5545 Size: 5551 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2160 2532 2817 3358 Size: 53 83 186 936 627 372 285 541 Cuts at: 3358 3740 4375 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 635 627 541 382 372 285 186 83 53 BsaWI w'CCGG_w Cuts at: 2204 2216 3984 4382 4656 4803 5214 2204 Size: 12 1768 398 274 147 411 2541 Fragments arranged by size: 2541 1768 411 398 274 147 12 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2648 2651 2678 2681 1178 Size: 30 165 30 1245 3 27 3 4048 Fragments arranged by size: 4048 1245 165 30 30 27 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2366 2627 2630 2672 2726 2729 1186 Size: 1180 261 3 42 54 3 4008 Fragments arranged by size: 4008 1180 261 54 42 3 3 BseYI C'CCAG_C Cuts at: 2332 2490 2504 2512 2675 4754 5250 2332 Size: 158 14 8 163 2079 496 2633 Fragments arranged by size: 2633 2079 496 163 158 14 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4978 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3001 3762 4790 1540 Size: 275 186 1000 761 1028 2301 Fragments arranged by size: 2301 1028 1000 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2484 3612 3697 4768 2484 Size: 1128 85 1071 3267 Fragments arranged by size: 3267 1128 1071 85 BsmI GAATG_Cn' Cuts at: 2423 3062 3155 2423 Size: 639 93 4819 Fragments arranged by size: 4819 639 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2484 2513 2570 2596 2634 2653 2836 Size: 399 694 29 57 26 38 19 183 Cuts at: 2836 2870 3612 3697 4768 1391 Size: 34 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 694 399 183 85 57 38 34 29 26 19 BspEI T'CCGG_A Cuts at: 2216 5214 2216 Size: 2998 2553 BspHI T'CATG_A Cuts at: 3440 5170 3440 Size: 1730 3821 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2906 844 Size: 2062 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2010 3438 1394 Size: 429 187 1428 3507 Fragments arranged by size: 3507 1428 429 187 BsrDI GCAATG_nn' Cuts at: 65 1248 4046 4220 65 Size: 1183 2798 174 1396 Fragments arranged by size: 2798 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2204 4195 4382 1700 Size: 110 394 1991 187 2869 Fragments arranged by size: 2869 1991 394 187 110 BsrGI T'GTAC_A Cuts at: 96 2379 96 Size: 2283 3268 BssHII G'CGCG_C Cuts at: 1925 2101 1925 Size: 176 5375 BssSI C'ACGA_G Cuts at: 3611 4623 3611 Size: 1012 4539 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5551 BstBI TT'CG_AA Cuts at: 4355 4355 Size: 5551 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5551 BstYI r'GATC_y Cuts at: 1972 2572 2881 3248 3633 3650 5102 5188 5200 Size: 600 309 367 385 17 1452 86 12 Cuts at: 5200 5546 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 600 385 367 346 309 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2168 513 Size: 552 1103 3896 Fragments arranged by size: 3896 1103 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4812 Fragments arranged by size: 4812 683 56 BtsI GCAGTG_nn' Cuts at: 3067 3861 3881 3067 Size: 794 20 4737 Fragments arranged by size: 4737 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5551 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5516 DraI TTT'AAA Cuts at: 2855 2855 Size: 5551 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2537 4558 809 Size: 507 1221 2021 1802 Fragments arranged by size: 2021 1802 1221 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2998 3887 5516 Size: 54 1425 50 275 18 1168 889 1629 Cuts at: 5516 8 Size: 43 Fragments arranged by size: 1629 1425 1168 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2998 1537 Size: 275 1186 4090 Fragments arranged by size: 4090 1186 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3481 1353 Size: 2128 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4138 4512 4658 387 Size: 1064 138 2549 374 146 1280 Fragments arranged by size: 2549 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2333 2392 2675 2774 3614 4211 4998 Size: 393 701 59 283 99 840 597 787 Cuts at: 4998 5266 1239 Size: 268 1524 Fragments arranged by size: 1524 840 787 701 597 393 283 268 99 59 EcoNI CCTnn'n_nnAGG Cuts at: 2402 2402 Size: 5551 EcoO109I rG'GnC_Cy Cuts at: 2140 2246 2361 2464 2593 2649 2650 2728 2758 Size: 106 115 103 129 56 1 78 30 Cuts at: 2758 2140 Size: 4933 Fragments arranged by size: 4933 129 115 106 103 78 56 30 1 EcoRI G'AATT_C Cuts at: 2860 3238 2860 Size: 378 5173 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2836 2836 Size: 5551 FspI TGC'GCA Cuts at: 4057 4057 Size: 5551 HaeII r_GCGC'y Cuts at: 1678 2092 2341 2440 4698 1678 Size: 414 249 99 2258 2531 Fragments arranged by size: 2531 2258 414 249 99 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2211 2243 2557 2589 4192 Size: 32 135 32 639 32 314 32 1603 Cuts at: 4192 4224 4266 4298 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1603 639 314 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2320 2895 3141 669 Size: 988 281 382 575 246 3079 Fragments arranged by size: 3079 988 575 382 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5551 HpaI GTT'AAC Cuts at: 3141 3141 Size: 5551 KpnI G_GTAC'C Cuts at: 2671 2876 2671 Size: 205 5346 MfeI C'AATT_G Cuts at: 3150 3150 Size: 5551 MluI A'CGCG_T Cuts at: 5427 5427 Size: 5551 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4665 4849 1305 Size: 3360 184 2007 Fragments arranged by size: 3360 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3729 Fragments arranged by size: 3729 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2297 2477 3509 3868 4027 518 Size: 648 1131 180 1032 359 159 2042 Fragments arranged by size: 2042 1131 1032 648 359 180 159 MspA1I CmG'CkG Cuts at: 1606 2263 2443 2590 2967 3644 4792 5037 1606 Size: 657 180 147 377 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 657 377 245 180 147 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4999 NdeI CA'TA_TG Cuts at: 387 387 Size: 5551 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5551 NotI GC'GGCC_GC Cuts at: 2998 2998 Size: 5551 NspI r_CATG'y Cuts at: 1132 1229 2380 2393 2909 4454 1132 Size: 97 1151 13 516 1545 2229 Fragments arranged by size: 2229 1545 1151 516 97 13 PasI CC'CwG_GG Cuts at: 1348 2497 1348 Size: 1149 4402 PciI A'CATG_T Cuts at: 4450 4450 Size: 5551 PflMI CCAn_nnn'nTGG Cuts at: 1108 2332 2497 1108 Size: 1224 165 4162 Fragments arranged by size: 4162 1224 165 PfoI T'CCnGG_A Cuts at: 2310 2754 2310 Size: 444 5107 PmeI GTTT'AAAC Cuts at: 2855 2855 Size: 5551 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3620 3652 5133 5165 3620 Size: 32 1481 32 4006 Fragments arranged by size: 4006 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2140 2464 2758 2140 Size: 324 294 4933 Fragments arranged by size: 4933 324 294 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5551 PsiI TTA'TAA Cuts at: 3121 3121 Size: 5551 PspOMI G'GGCC_C Cuts at: 2592 2649 2592 Size: 57 5494 PstI C_TGCA'G Cuts at: 830 2279 2349 2903 830 Size: 1449 70 554 3478 Fragments arranged by size: 3478 1449 554 70 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5551 PvuII CAG'CTG Cuts at: 2263 2443 2263 Size: 180 5371 SalI G'TCGA_C Cuts at: 1936 2893 1936 Size: 957 4594 SbfI CC_TGCA'GG Cuts at: 2903 2903 Size: 5551 ScaI AGT'ACT Cuts at: 3799 5511 3799 Size: 1712 3839 SfcI C'TryA_G Cuts at: 826 1045 2275 2345 2899 4034 4715 4906 826 Size: 219 1230 70 554 1135 681 191 1471 Fragments arranged by size: 1471 1230 1135 681 554 219 191 70 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5551 SgrAI Cr'CCGG_yG Cuts at: 1700 2204 1700 Size: 504 5047 SmaI CCC'GGG Cuts at: 1872 2058 2878 1872 Size: 186 820 4545 Fragments arranged by size: 4545 820 186 SmlI C'TyrA_G Cuts at: 820 1017 1943 3655 4556 4818 820 Size: 197 926 1712 901 262 1553 Fragments arranged by size: 1712 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3342 493 Size: 2849 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5551 SphI G_CATG'C Cuts at: 1132 2909 1132 Size: 1777 3774 SspI AAT'ATT Cuts at: 5 52 3475 5 Size: 47 3423 2081 Fragments arranged by size: 3423 2081 47 StuI AGG'CCT Cuts at: 1517 2623 1517 Size: 1106 4445 StyI C'CwwG_G Cuts at: 513 1065 2990 513 Size: 552 1925 3074 Fragments arranged by size: 3074 1925 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2489 3578 3595 3748 3933 4378 325 Size: 1255 909 1089 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1089 909 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2379 3797 5509 Size: 274 80 33 51 1443 402 1418 1712 Cuts at: 5509 96 Size: 138 Fragments arranged by size: 1712 1443 1418 402 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2648 2680 5511 5543 2648 Size: 32 2831 32 2656 Fragments arranged by size: 2831 2656 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5551 XbaI T'CTAG_A Cuts at: 2887 2887 Size: 5551 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2692 2692 Size: 5551 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5551 XmaI C'CCGG_G Cuts at: 1870 2056 2876 1870 Size: 186 820 4545 Fragments arranged by size: 4545 820 186 XmnI GAAnn'nnTTC Cuts at: 3680 3680 Size: 5551 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3359 276 Size: 53 83 186 936 1825 2468 Fragments arranged by size: 2468 1825 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI