(Linear) (Six-base) MAPSORT of: KIEE1775.seq Check: 5041 from: 1 to: 846 KIEE1775.orf With 182 enzymes: SgfI * July 15, 2010 18:47 .. Acc65I G'GTAC_C Cuts at: 0 662 846 Size: 662 184 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 769 846 Size: 769 77 AfeI AGC'GCT Cuts at: 0 85 846 Size: 85 761 AgeI A'CCGG_T Cuts at: 0 199 846 Size: 199 647 AlwNI CAG_nnn'CTG Cuts at: 0 40 269 327 492 703 846 Size: 40 229 58 165 211 143 Fragments arranged by size: 229 211 165 143 58 40 ApaI G_GGCC'C Cuts at: 0 591 648 846 Size: 591 57 198 Fragments arranged by size: 591 198 57 ApaLI G'TGCA_C Cuts at: 0 475 846 Size: 475 371 AvaI C'yCGr_G Cuts at: 0 51 846 Size: 51 795 BanI G'GyrC_C Cuts at: 0 626 662 693 846 Size: 626 36 31 153 Fragments arranged by size: 626 153 36 31 BanII G_rGCy'C Cuts at: 0 508 565 591 648 831 846 Size: 508 57 26 57 183 15 Fragments arranged by size: 508 183 57 57 26 15 BbsI GAAGACnn'nnnn_ Cuts at: 0 807 846 Size: 807 39 BbvCI CC'TCA_GC Cuts at: 0 613 846 Size: 613 233 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 207 241 846 Size: 207 34 605 Fragments arranged by size: 605 207 34 BglI GCCn_nnn'nGGC Cuts at: 0 624 846 Size: 624 222 BglII A'GATC_T Cuts at: 0 567 846 Size: 567 279 Bme1580I G_kGCm'C Cuts at: 0 479 591 629 648 846 Size: 479 112 38 19 198 Fragments arranged by size: 479 198 112 38 19 BmgBI CAC'GTC Cuts at: 0 474 846 Size: 474 372 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 527 846 Size: 527 319 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 328 387 670 846 Size: 328 59 283 176 Fragments arranged by size: 328 283 176 59 Bpu10I CC'TnA_GC Cuts at: 0 613 846 Size: 613 233 BsaI GGTCTCn'nnnn_ Cuts at: 0 540 846 Size: 540 306 BsaHI Gr'CG_yC Cuts at: 0 155 527 812 846 Size: 155 372 285 34 Fragments arranged by size: 372 285 155 34 BsaWI w'CCGG_w Cuts at: 0 199 211 846 Size: 199 12 635 Fragments arranged by size: 635 199 12 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 643 646 673 676 846 Size: 643 3 27 3 170 Fragments arranged by size: 643 170 27 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 361 622 625 667 721 724 846 Size: 361 261 3 42 54 3 122 Fragments arranged by size: 361 261 122 54 42 3 3 BseYI C'CCAG_C Cuts at: 0 327 485 499 507 670 846 Size: 327 158 14 8 163 176 Fragments arranged by size: 327 176 163 158 14 8 BsiHKAI G_wGCw'C Cuts at: 0 479 846 Size: 479 367 BsmI GAATG_Cn' Cuts at: 0 418 846 Size: 418 428 Bsp1286I G_dGCh'C Cuts at: 0 479 508 565 591 629 648 831 846 Size: 479 29 57 26 38 19 183 15 Fragments arranged by size: 479 183 57 38 29 26 19 15 BspEI T'CCGG_A Cuts at: 0 211 846 Size: 211 635 BsrBI CCG'CTC Cuts at: 0 5 846 Size: 5 841 BsrFI r'CCGG_y Cuts at: 0 199 846 Size: 199 647 BsrGI T'GTAC_A Cuts at: 0 374 846 Size: 374 472 BssHII G'CGCG_C Cuts at: 0 96 846 Size: 96 750 BstYI r'GATC_y Cuts at: 0 567 846 Size: 567 279 BtgI C'CryG_G Cuts at: 0 163 846 Size: 163 683 DrdI GACnn_nn'nnGTC Cuts at: 0 532 846 Size: 532 314 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 328 387 670 769 846 Size: 328 59 283 99 77 Fragments arranged by size: 328 283 99 77 59 EcoNI CCTnn'n_nnAGG Cuts at: 0 397 846 Size: 397 449 EcoO109I rG'GnC_Cy Cuts at: 0 135 241 356 459 588 644 645 723 Size: 135 106 115 103 129 56 1 78 Cuts at: 723 753 846 Size: 30 93 Fragments arranged by size: 135 129 115 106 103 93 78 56 30 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 831 846 Size: 831 15 HaeII r_GCGC'y Cuts at: 0 87 336 435 846 Size: 87 249 99 411 Fragments arranged by size: 411 249 99 87 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 206 238 552 584 846 Size: 206 32 314 32 262 Fragments arranged by size: 314 262 206 32 32 HincII GTy'rAC Cuts at: 0 315 846 Size: 315 531 KpnI G_GTAC'C Cuts at: 0 666 846 Size: 666 180 MslI CAynn'nnrTG Cuts at: 0 292 472 846 Size: 292 180 374 Fragments arranged by size: 374 292 180 MspA1I CmG'CkG Cuts at: 0 258 438 585 846 Size: 258 180 147 261 Fragments arranged by size: 261 258 180 147 NspI r_CATG'y Cuts at: 0 375 388 846 Size: 375 13 458 Fragments arranged by size: 458 375 13 PasI CC'CwG_GG Cuts at: 0 492 846 Size: 492 354 PflMI CCAn_nnn'nTGG Cuts at: 0 327 492 846 Size: 327 165 354 Fragments arranged by size: 354 327 165 PfoI T'CCnGG_A Cuts at: 0 305 749 846 Size: 305 444 97 Fragments arranged by size: 444 305 97 PpuMI rG'GwC_Cy Cuts at: 0 135 459 753 846 Size: 135 324 294 93 Fragments arranged by size: 324 294 135 93 PspOMI G'GGCC_C Cuts at: 0 587 644 846 Size: 587 57 202 Fragments arranged by size: 587 202 57 PstI C_TGCA'G Cuts at: 0 274 344 846 Size: 274 70 502 Fragments arranged by size: 502 274 70 PvuII CAG'CTG Cuts at: 0 258 438 846 Size: 258 180 408 Fragments arranged by size: 408 258 180 SfcI C'TryA_G Cuts at: 0 270 340 846 Size: 270 70 506 Fragments arranged by size: 506 270 70 SgrAI Cr'CCGG_yG Cuts at: 0 199 846 Size: 199 647 SmaI CCC'GGG Cuts at: 0 53 846 Size: 53 793 StuI AGG'CCT Cuts at: 0 618 846 Size: 618 228 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 484 846 Size: 484 362 TatI w'GTAC_w Cuts at: 0 374 846 Size: 374 472 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 643 675 846 Size: 643 32 171 Fragments arranged by size: 643 171 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 687 846 Size: 687 159 XmaI C'CCGG_G Cuts at: 0 51 846 Size: 51 795 Enzymes that do cut: Acc65I AcuI AfeI AgeI AlwNI ApaI ApaLI AvaI BanI BanII BbsI BbvCI BcgI BglI BglII Bme1580I BmgBI BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI BsrBI BsrFI BsrGI BssHII BstYI BtgI DrdI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KpnI MslI MspA1I NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SfcI SgrAI SmaI StuI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AccI AclI AflII AflIII AhdI AleI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BclI BfrBI BlpI BmrI BmtI BpuEI BsaAI BsaBI BsgI BsiEI BsiWI BsmBI BspHI BspMI BsrDI BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EaeI EagI EarI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI MfeI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI Tth111I XbaI XhoI XmnI ZraI