(Circular) (Six-base) MAPSORT of: pFN21AE2381.seq Check: 8988 from: 1 to: 9175 pFN21AE2381 9175 bp With 182 enzymes: SgfI * October 25, 2011 13:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5958 5958 Size: 9175 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5152 6985 278 Size: 53 83 186 936 3616 1833 2468 Fragments arranged by size: 3616 2468 1833 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4948 6518 1937 Size: 3011 1570 4594 Fragments arranged by size: 4594 3011 1570 Acc65I G'GTAC_C Cuts at: 4704 6496 4704 Size: 1792 7383 AclI AA'CG_TT Cuts at: 1563 3881 4738 7302 7675 1563 Size: 2318 857 2564 373 3063 Fragments arranged by size: 3063 2564 2318 857 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2594 3414 4289 4683 5559 5949 6287 7238 Size: 962 820 875 394 876 390 338 951 Cuts at: 7238 8622 8890 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 962 951 876 875 820 394 390 338 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8978 AflIII A'CryG_T Cuts at: 1203 3989 8074 9051 1203 Size: 2786 4085 977 1327 Fragments arranged by size: 4085 2786 1327 977 AgeI A'CCGG_T Cuts at: 8006 8006 Size: 9175 AhdI GACnn_n'nnGTC Cuts at: 7904 7904 Size: 9175 AleI CACnn'nnGTG Cuts at: 1166 4356 1166 Size: 3190 5985 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9175 AlwNI CAG_nnn'CTG Cuts at: 3230 3383 5452 6112 6918 8490 3230 Size: 153 2069 660 806 1572 3915 Fragments arranged by size: 3915 2069 1572 806 660 153 ApaI G_GGCC'C Cuts at: 5727 6389 5727 Size: 662 8513 ApaLI G'TGCA_C Cuts at: 4490 5029 7232 8388 4490 Size: 539 2203 1156 5277 Fragments arranged by size: 5277 2203 1156 539 ApoI r'AATT_y Cuts at: 1493 3424 3802 4113 4564 4958 6484 6710 6862 Size: 1931 378 311 451 394 1526 226 152 Cuts at: 6862 8971 1493 Size: 2109 1697 Fragments arranged by size: 2109 1931 1697 1526 451 394 378 311 226 152 AseI AT'TA_AT Cuts at: 160 1032 7729 160 Size: 872 6697 1606 Fragments arranged by size: 6697 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9175 AvaI C'yCGr_G Cuts at: 1870 1943 2361 2716 2770 2812 6500 1870 Size: 73 418 355 54 42 3688 4545 Fragments arranged by size: 4545 3688 418 355 73 54 42 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3825 3858 3825 Size: 33 9142 BamHI G'GATC_C Cuts at: 6505 6872 6505 Size: 367 8808 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4424 4704 6496 7951 618 Size: 325 217 627 2637 280 1792 1455 1842 Fragments arranged by size: 2637 1842 1792 1455 627 325 280 217 BanII G_rGCy'C Cuts at: 1391 2672 2885 4029 5727 5811 6389 6494 1391 Size: 1281 213 1144 1698 84 578 105 4072 Fragments arranged by size: 4072 1698 1281 1144 578 213 105 84 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2558 3839 4147 6390 928 Size: 971 659 1281 308 2243 3713 Fragments arranged by size: 3713 2243 1281 971 659 308 BbvCI CC'TCA_GC Cuts at: 4086 5265 6273 4086 Size: 1179 1008 6988 Fragments arranged by size: 6988 1179 1008 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2318 2352 7366 7400 1903 Size: 34 50 34 297 34 5014 34 3678 Fragments arranged by size: 5014 3678 297 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7067 8277 7067 Size: 1210 7965 BclI T'GATC_A Cuts at: 1548 4845 1548 Size: 3297 5878 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5722 7786 136 Size: 107 122 71 631 605 4050 2064 1525 Fragments arranged by size: 4050 2064 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2903 4039 9170 2903 Size: 1136 5131 2908 Fragments arranged by size: 5131 2908 1136 BlpI GC'TnA_GC Cuts at: 4833 6592 4833 Size: 1759 7416 Bme1580I G_kGCm'C Cuts at: 1790 2198 2586 4494 5033 5482 5727 6179 6389 Size: 408 388 1908 539 449 245 452 210 Cuts at: 6389 7236 8392 1790 Size: 847 1156 2573 Fragments arranged by size: 2573 1908 1156 847 539 452 449 408 388 245 210 BmgBI CAC'GTC Cuts at: 1318 3992 1318 Size: 2674 6501 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2970 3042 4168 Size: 416 53 83 399 24 1553 72 1126 Cuts at: 4168 5555 6357 6388 6906 7864 442 Size: 1387 802 31 518 958 1753 Fragments arranged by size: 1753 1553 1387 1126 958 802 518 416 399 83 72 53 31 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9175 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3215 5415 3215 Size: 2200 6975 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2398 3936 4005 4044 4698 5079 5705 7835 Size: 1159 1538 69 39 654 381 626 2130 Cuts at: 7835 1239 Size: 2579 Fragments arranged by size: 2579 2130 1538 1159 654 626 381 69 39 Bpu10I CC'TnA_GC Cuts at: 3014 3315 4086 5265 5390 5805 6232 6273 6361 Size: 301 771 1179 125 415 427 41 88 Cuts at: 6361 3014 Size: 5828 Fragments arranged by size: 5828 1179 771 427 415 301 125 88 41 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3822 5665 5992 7300 8165 8463 3822 Size: 1843 327 1308 865 298 4534 Fragments arranged by size: 4534 1843 1308 865 327 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3030 3569 4099 5510 5968 6299 7838 Size: 617 1531 539 530 1411 458 331 1539 Cuts at: 7838 882 Size: 2219 Fragments arranged by size: 2219 1539 1531 1411 617 539 530 458 331 BsaAI yAC'GTr Cuts at: 493 1204 6966 493 Size: 711 5762 2702 Fragments arranged by size: 5762 2702 711 BsaBI GATnn'nnATC Cuts at: 9169 9169 Size: 9175 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4945 5149 6982 7364 Size: 53 83 186 936 3412 204 1833 382 Cuts at: 7364 7999 275 Size: 635 1451 Fragments arranged by size: 3412 1833 1451 936 635 382 204 186 83 53 BsaWI w'CCGG_w Cuts at: 2439 2592 7608 8006 8280 8427 8838 2439 Size: 153 5016 398 274 147 411 2776 Fragments arranged by size: 5016 2776 411 398 274 153 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2237 2267 2561 2587 2591 Size: 30 165 30 834 30 294 26 4 Cuts at: 2591 2617 3619 3649 3871 3901 4245 4275 5411 Size: 26 1002 30 222 30 344 30 1136 Cuts at: 5411 5441 1178 Size: 30 4912 Fragments arranged by size: 4912 1136 1002 834 344 294 222 165 30 30 30 30 30 30 30 26 26 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2064 2486 3548 5082 5546 5763 5768 6477 Size: 878 422 1062 1534 464 217 5 709 Cuts at: 6477 1186 Size: 3884 Fragments arranged by size: 3884 1534 1062 878 709 464 422 217 5 BseYI C'CCAG_C Cuts at: 5254 5297 5499 5887 5922 6331 8378 8874 5254 Size: 43 202 388 35 409 2047 496 5555 Fragments arranged by size: 5555 2047 496 409 388 202 43 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2312 6209 1163 Size: 573 576 3897 4129 Fragments arranged by size: 4129 3897 576 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2323 2933 6625 7386 8414 1540 Size: 275 186 322 610 3692 761 1028 2301 Fragments arranged by size: 3692 2301 1028 761 610 322 275 186 BsiHKAI G_wGCw'C Cuts at: 4029 4494 5033 5964 7236 7321 8392 4029 Size: 465 539 931 1272 85 1071 4812 Fragments arranged by size: 4812 1272 1071 931 539 465 85 BsmI GAATG_Cn' Cuts at: 6686 6779 6686 Size: 93 9082 BsmBI CGTCTCn'nnnn_ Cuts at: 2920 2920 Size: 9175 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2198 2586 2672 2885 4029 4494 5033 Size: 399 408 388 86 213 1144 465 539 Cuts at: 5033 5482 5727 5811 5964 6179 6389 6494 7236 Size: 449 245 84 153 215 210 105 742 Cuts at: 7236 7321 8392 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1144 1071 742 539 465 449 408 399 388 245 215 213 210 153 105 86 85 84 BspEI T'CCGG_A Cuts at: 8838 8838 Size: 9175 BspHI T'CATG_A Cuts at: 4521 7064 8794 4521 Size: 2543 1730 4902 Fragments arranged by size: 4902 2543 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2488 5376 5958 6530 844 Size: 1644 2888 582 572 3489 Fragments arranged by size: 3489 2888 1644 582 572 BsrBI CCG'CTC Cuts at: 1394 1823 7062 1394 Size: 429 5239 3507 Fragments arranged by size: 5239 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 7670 7844 65 Size: 1183 6422 174 1396 Fragments arranged by size: 6422 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7819 8006 1700 Size: 110 6009 187 2869 Fragments arranged by size: 6009 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3817 96 Size: 3721 5454 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9175 BssSI C'ACGA_G Cuts at: 7235 8247 7235 Size: 1012 8163 BstAPI GCAn_nnn'nTGC Cuts at: 794 3230 5555 794 Size: 2436 2325 4414 Fragments arranged by size: 4414 2436 2325 BstBI TT'CG_AA Cuts at: 2465 7979 2465 Size: 5514 3661 BstEII G'GTnAC_C Cuts at: 1184 2170 2874 1184 Size: 986 704 7485 Fragments arranged by size: 7485 986 704 BstXI CCAn_nnnn'nTGG Cuts at: 2161 4881 2161 Size: 2720 6455 BstYI r'GATC_y Cuts at: 1972 2660 2903 3125 4039 5014 6505 6872 7257 Size: 688 243 222 914 975 1491 367 385 Cuts at: 7257 7274 8726 8812 8824 9170 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1491 1452 975 914 688 385 367 346 243 222 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5218 5218 Size: 9175 BtgI C'CryG_G Cuts at: 513 1065 5520 513 Size: 552 4455 4168 Fragments arranged by size: 4455 4168 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8436 Fragments arranged by size: 8436 683 56 BtsI GCAGTG_nn' Cuts at: 5361 6691 7485 7505 5361 Size: 1330 794 20 7031 Fragments arranged by size: 7031 1330 794 20 ClaI AT'CG_AT Cuts at: 1554 2429 1554 Size: 875 8300 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5692 5727 529 Size: 35 5128 35 3977 Fragments arranged by size: 5128 3977 35 35 DraI TTT'AAA Cuts at: 6479 6479 Size: 9175 DraIII CAC_nnn'GTG Cuts at: 6247 6247 Size: 9175 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8182 809 Size: 507 6866 1802 Fragments arranged by size: 6866 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2731 4219 5944 Size: 54 1425 50 275 18 901 1488 1725 Cuts at: 5944 6622 7511 9140 8 Size: 678 889 1629 43 Fragments arranged by size: 1725 1629 1488 1425 901 889 678 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6622 1537 Size: 275 4810 4090 Fragments arranged by size: 4810 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3116 3594 3687 3954 4659 5172 5789 6448 Size: 1763 478 93 267 705 513 617 659 Cuts at: 6448 7105 1353 Size: 657 3423 Fragments arranged by size: 3423 1763 705 659 657 617 513 478 267 93 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7762 8136 8282 387 Size: 1064 138 6173 374 146 1280 Fragments arranged by size: 6173 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4027 4027 Size: 9175 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2398 2594 3414 3936 4005 4044 4289 Size: 393 766 196 820 522 69 39 245 Cuts at: 4289 4683 4698 5079 5559 5705 5949 6287 7238 Size: 394 15 381 480 146 244 338 951 Cuts at: 7238 7835 8622 8890 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 951 820 787 766 597 522 480 394 393 381 338 268 245 244 196 146 69 39 15 EcoNI CCTnn'n_nnAGG Cuts at: 2815 2890 2920 6039 2815 Size: 75 30 3119 5951 Fragments arranged by size: 5951 3119 75 30 EcoO109I rG'GnC_Cy Cuts at: 5723 5724 5799 6126 6208 6226 6385 6425 5723 Size: 1 75 327 82 18 159 40 8473 Fragments arranged by size: 8473 327 159 82 75 40 18 1 EcoRI G'AATT_C Cuts at: 4958 6484 6862 4958 Size: 1526 378 7271 Fragments arranged by size: 7271 1526 378 EcoRV GAT'ATC Cuts at: 2230 2445 2230 Size: 215 8960 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2973 3965 5876 2973 Size: 992 1911 6272 Fragments arranged by size: 6272 1911 992 FspI TGC'GCA Cuts at: 7681 7681 Size: 9175 HaeII r_GCGC'y Cuts at: 1678 5871 8322 1678 Size: 4193 2451 2531 Fragments arranged by size: 4193 2531 2451 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2219 2237 2251 2269 2429 Size: 32 135 32 647 18 14 18 160 Cuts at: 2429 2461 2561 2587 2593 2619 2699 2731 3022 Size: 32 100 26 6 26 80 32 291 Cuts at: 3022 3054 3446 3478 3529 3561 3976 4008 4999 Size: 32 392 32 51 32 415 32 991 Cuts at: 4999 5031 7816 7848 7890 7922 1373 Size: 32 2785 32 42 32 2626 Fragments arranged by size: 2785 2626 991 647 415 392 291 160 135 100 80 51 42 32 32 32 32 32 32 32 32 32 32 32 26 26 18 18 14 6 HincII GTy'rAC Cuts at: 669 1657 1938 4366 6519 6765 669 Size: 988 281 2428 2153 246 3079 Fragments arranged by size: 3079 2428 2153 988 281 246 HindIII A'AGCT_T Cuts at: 748 5598 748 Size: 4850 4325 HpaI GTT'AAC Cuts at: 4366 6765 4366 Size: 2399 6776 KpnI G_GTAC'C Cuts at: 4708 6500 4708 Size: 1792 7383 MfeI C'AATT_G Cuts at: 3597 6774 3597 Size: 3177 5998 MluI A'CGCG_T Cuts at: 9051 9051 Size: 9175 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2268 3014 3432 5454 8289 8473 1305 Size: 963 746 418 2022 2835 184 2007 Fragments arranged by size: 2835 2022 2007 963 746 418 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2733 4221 5946 10 Size: 54 1425 343 901 1488 1725 3239 Fragments arranged by size: 3239 1725 1488 1425 901 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4356 7133 7492 7651 518 Size: 648 3190 2777 359 159 2042 Fragments arranged by size: 3190 2777 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2964 3227 3854 4877 5036 5335 5413 5452 Size: 1358 263 627 1023 159 299 78 39 Cuts at: 5452 5615 5653 6591 7268 8416 8661 1606 Size: 163 38 938 677 1148 245 2120 Fragments arranged by size: 2120 1358 1148 1023 938 677 627 299 263 245 163 159 78 39 38 NcoI C'CATG_G Cuts at: 513 1065 5520 513 Size: 552 4455 4168 Fragments arranged by size: 4455 4168 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9175 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9175 NotI GC'GGCC_GC Cuts at: 6622 6622 Size: 9175 NspI r_CATG'y Cuts at: 1132 1229 5005 5623 5769 6302 6533 8078 1132 Size: 97 3776 618 146 533 231 1545 2229 Fragments arranged by size: 3776 2229 1545 618 533 231 146 97 PasI CC'CwG_GG Cuts at: 1348 2191 1348 Size: 843 8332 PciI A'CATG_T Cuts at: 8074 8074 Size: 9175 PflMI CCAn_nnn'nTGG Cuts at: 1108 6112 1108 Size: 5004 4171 PfoI T'CCnGG_A Cuts at: 2663 2800 2663 Size: 137 9038 PmeI GTTT'AAAC Cuts at: 6479 6479 Size: 9175 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3115 3147 7244 7276 8757 8789 3115 Size: 32 4097 32 1481 32 3501 Fragments arranged by size: 4097 3501 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 6126 6208 6226 6126 Size: 82 18 9075 Fragments arranged by size: 9075 82 18 PshAI GACnn'nnGTC Cuts at: 1613 2298 2748 2932 1613 Size: 685 450 184 7856 Fragments arranged by size: 7856 685 450 184 PsiI TTA'TAA Cuts at: 6745 6745 Size: 9175 PspOMI G'GGCC_C Cuts at: 5723 6385 5723 Size: 662 8513 PspXI vC'TCGA_Gb Cuts at: 2770 2770 Size: 9175 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5031 5063 5031 Size: 32 9143 PstI C_TGCA'G Cuts at: 830 3241 4981 5376 6527 830 Size: 2411 1740 395 1151 3478 Fragments arranged by size: 3478 2411 1740 1151 395 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9175 PvuII CAG'CTG Cuts at: 3227 3854 4877 5036 5335 5413 5452 5615 5653 Size: 627 1023 159 299 78 39 163 38 Cuts at: 5653 3227 Size: 6749 Fragments arranged by size: 6749 1023 627 299 163 159 78 39 38 SacI G_AGCT'C Cuts at: 4029 4029 Size: 9175 SalI G'TCGA_C Cuts at: 1936 6517 1936 Size: 4581 4594 SanDI GG'GwC_CC Cuts at: 6226 6226 Size: 9175 SapI GCTCTTCn'nnn_ Cuts at: 3687 5172 3687 Size: 1485 7690 SbfI CC_TGCA'GG Cuts at: 6527 6527 Size: 9175 ScaI AGT'ACT Cuts at: 3745 5230 5987 7423 9135 3745 Size: 1485 757 1436 1712 3785 Fragments arranged by size: 3785 1712 1485 1436 757 SfcI C'TryA_G Cuts at: 826 1045 2887 2917 3237 4977 5048 5372 6523 Size: 219 1842 30 320 1740 71 324 1151 Cuts at: 6523 7658 8339 8530 826 Size: 1135 681 191 1471 Fragments arranged by size: 1842 1740 1471 1151 1135 681 324 320 219 191 71 30 SfiI GGCCn_nnn'nGGCC Cuts at: 5722 5722 Size: 9175 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9175 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9175 SmaI CCC'GGG Cuts at: 1872 2363 6502 1872 Size: 491 4139 4545 Fragments arranged by size: 4545 4139 491 SmlI C'TyrA_G Cuts at: 820 1017 1943 2770 2812 3837 5644 5971 7279 Size: 197 926 827 42 1025 1807 327 1308 Cuts at: 7279 8180 8442 820 Size: 901 262 1553 Fragments arranged by size: 1807 1553 1308 1025 926 901 827 327 262 197 42 SnaBI TAC'GTA Cuts at: 493 6966 493 Size: 6473 2702 SpeI A'CTAG_T Cuts at: 152 4258 152 Size: 4106 5069 SphI G_CATG'C Cuts at: 1132 6533 1132 Size: 5401 3774 SspI AAT'ATT Cuts at: 5 52 7099 5 Size: 47 7047 2081 Fragments arranged by size: 7047 2081 47 StuI AGG'CCT Cuts at: 1517 5313 5969 6372 1517 Size: 3796 656 403 4320 Fragments arranged by size: 4320 3796 656 403 StyI C'CwwG_G Cuts at: 513 1065 3264 4236 5520 6099 6151 6614 513 Size: 552 2199 972 1284 579 52 463 3074 Fragments arranged by size: 3074 2199 1284 972 579 552 463 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2295 2402 2638 2815 2989 7202 7219 Size: 1255 715 107 236 177 174 4213 17 Cuts at: 7219 7372 7557 8002 325 Size: 153 185 445 1498 Fragments arranged by size: 4213 1498 1255 715 445 236 185 177 174 153 107 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3743 3817 5228 Size: 274 80 33 51 1443 1766 74 1411 Cuts at: 5228 5985 7421 9133 96 Size: 757 1436 1712 138 Fragments arranged by size: 1766 1712 1443 1436 1411 757 274 138 80 74 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2342 2374 3994 4026 4156 4188 4245 4277 4571 Size: 32 1620 32 130 32 57 32 294 Cuts at: 4571 4603 9135 9167 2342 Size: 32 4532 32 2350 Fragments arranged by size: 4532 2350 1620 294 130 57 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 6000 1316 Size: 4684 4491 XbaI T'CTAG_A Cuts at: 6511 6511 Size: 9175 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4825 4825 Size: 9175 XhoI C'TCGA_G Cuts at: 1943 2770 2812 1943 Size: 827 42 8306 Fragments arranged by size: 8306 827 42 XmaI C'CCGG_G Cuts at: 1870 2361 6500 1870 Size: 491 4139 4545 Fragments arranged by size: 4545 4139 491 XmnI GAAnn'nnTTC Cuts at: 3948 7304 3948 Size: 3356 5819 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5150 6983 276 Size: 53 83 186 936 3616 1833 2468 Fragments arranged by size: 3616 2468 1833 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI RsrII SacII SexAI SfoI SrfI SwaI