################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccI AccII AcyI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BclI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseRI BsgI BsiEI BsiHKAI BsmAI BsmBI BsoBI BspDI BspHI BspLU11I BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstYI BstZ17I CelII CfoI ClaI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MslI MunI MvnI NaeI NarI NdeI NgoMIV NotI NruI PacI PaeR7I PflMI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI RcaI SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI SwaI Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccIII 365 TCCGGA T^CCGGA AclI 116 AACGTT AA^CGTT AcsI 145 AAATTT [GA]^AATT[CT] AflII 829 CTTAAG C^TTAAG AflIII 620 ACGTGT A^C[GA][CT]GT AgeI 677 ACCGGT A^CCGGT AlwI 843 GATCC GGATC(4/5) AlwNI 658 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 271 CCTGAGG CC^T[ACGT]AGG ApoI 145 AAATTT [GA]^AATT[CT] AspI 78 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 216 GGACC G^G[AT]CC BalI 665 TGGCCA TGG^CCA BanI 807 GGCACC G^G[CT][GA]CC BanII 568 GAGCCC G[GA]GC[CT]^C BbsI 339 GAAGAC GAAGAC(2/6) BfaI 913 CTAG C^TAG BfrI 829 CTTAAG C^TTAAG BpuAI 339 GAAGAC GAAGAC(2/6) BseAI 365 TCCGGA T^CCGGA BsiWI 639 CGTACG C^GTACG BsmI 779 GAATGC GAATGC(1/-1) Bsp1407I 238 TGTACA T^GTACA BspEI 365 TCCGGA T^CCGGA BsrGI 238 TGTACA T^GTACA BstBI 742 TTCGAA TT^CGAA BstXI 224 CCATACATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 271 CCTGAGG CC^T[ACGT]AGG CpoI 215 CGGACCG CG^G[AT]CCG DsaI 802 CCATGG C^C[GA][CT]GG EarI 880 GAAGAG CTCTTC(1/4) Eco81I 271 CCTGAGG CC^T[ACGT]AGG EcoRV 427 GATATC GAT^ATC EcoT22I 781 ATGCAT ATGCA^T HincII 753 GTTGAC GT[CT]^[GA]AC HindII 753 GTTGAC GT[CT]^[GA]AC Ksp632I 880 GAAGAG CTCTTC(1/4) MaeI 913 CTAG C^TAG MluNI 665 TGGCCA TGG^CCA MroI 365 TCCGGA T^CCGGA MscI 665 TGGCCA TGG^CCA NcoI 802 CCATGG C^CATGG NheI 912 GCTAGC G^CTAGC NlaIV 807 GGCACC GG[ACGT]^[ACGT]CC NsiI 781 ATGCAT ATGCA^T NspV 742 TTCGAA TT^CGAA PflFI 78 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 677 ACCGGT A^CCGGT Psp1406I 116 AACGTT AA^CGTT RsrII 215 CGGACCG CG^G[AT]CCG SfcI 92 CTATAG C^T[GA][CT]AG SfuI 742 TTCGAA TT^CGAA SphI 644 GCATGC GCATG^C SspBI 238 TGTACA T^GTACA TfiI 559 GAATC G^A[AT]TC Tsp45I 76 GTGAC ^GT[GC]AC Tsp509I 146 AATT ^AATT TspRI 499 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 78 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AciI 86 CCGC CCGC(-3/-1) 543 CCGC BbvCI 853 CCTCAGC CCTCAGC(-5/-2) 907 CCTCAGC BcnI 179 CCCGG CC^[GC]GG 653 CCCGG BmyI 568 GAGCCC G[AGT]GC[ACT]^C 806 GGGCAC BsaWI 365 TCCGGA [AT]^CCGG[AT] 677 ACCGGT BsmFI 309 GGGAC GGGAC(10/14) 521 GGGAC Bsp1286I 568 GAGCCC G[AGT]GC[ACT]^C 806 GGGCAC BsrFI 677 ACCGGT [GA]^CCGG[CT] 686 GCCGGT BsrI 67 CCAGT ACTGG(1/-1) 100 CCAGT BstF5I 825 CATCC GGATG(2/0) 720 GGATG Cfr10I 677 ACCGGT [GA]^CCGG[CT] 686 GCCGGT DraI 148 TTTAAA TTT^AAA 394 TTTAAA FokI 825 CATCC GGATG(9/13) 720 GGATG MaeII 117 ACGT A^CGT 620 ACGT MaeIII 76 GTGAC ^GT[ACGT]AC 490 GTAAC NciI 179 CCCGG CC^[GC]GG 653 CCCGG NspI 174 ACATGC [GA]CATG^[CT] 644 GCATGC PleI 78 GACTC GAGTC(4/5) 660 GACTC PvuII 242 CAGCTG CAG^CTG 856 CAGCTG SmlI 17 CTCAAG C^T[CT][GA]AG 829 CTTAAG TaqI 37 TCGA T^CGA 743 TCGA TthHB8I 37 TCGA T^CGA 743 TCGA XcmI 508 CCAGACATGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 716 CCAAGGATGTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AfaI AluI BbvI BpmI BsaJI BsiYI BslI BssKI BstNI Cac8I Cfr13I DdeI DpnII EaeI EcoRII EcoT14I Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII RsaI Sau3AI Sau96I ScrFI SfaNI StyI Tru9I TseI