(Circular) (Six-base) MAPSORT of: pFN21AE6289.seq Check: 6090 from: 1 to: 5623 pFN21AE6289 5623 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3433 278 Size: 53 83 186 936 1897 2468 Fragments arranged by size: 2468 1897 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2966 1937 Size: 1029 4594 Acc65I G'GTAC_C Cuts at: 2944 2944 Size: 5623 AclI AA'CG_TT Cuts at: 1563 2122 3750 4123 1563 Size: 559 1628 373 3063 Fragments arranged by size: 3063 1628 559 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3686 5070 5338 1632 Size: 2054 1384 268 1917 Fragments arranged by size: 2054 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2834 820 Size: 197 1817 3609 Fragments arranged by size: 3609 1817 197 AflIII A'CryG_T Cuts at: 1203 2625 4522 5499 1203 Size: 1422 1897 977 1327 Fragments arranged by size: 1897 1422 1327 977 AgeI A'CCGG_T Cuts at: 2682 4454 2682 Size: 1772 3851 AhdI GACnn_n'nnGTC Cuts at: 4352 4352 Size: 5623 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5623 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5623 AlwNI CAG_nnn'CTG Cuts at: 2668 3366 4938 2668 Size: 698 1572 3353 Fragments arranged by size: 3353 1572 698 ApaLI G'TGCA_C Cuts at: 3680 4836 3680 Size: 1156 4467 ApoI r'AATT_y Cuts at: 1493 2150 2932 3158 3310 5419 1493 Size: 657 782 226 152 2109 1697 Fragments arranged by size: 2109 1697 782 657 226 152 AseI AT'TA_AT Cuts at: 160 1032 4177 160 Size: 872 3145 1606 Fragments arranged by size: 3145 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5623 AvaI C'yCGr_G Cuts at: 1870 1943 2948 1870 Size: 73 1005 4545 Fragments arranged by size: 4545 1005 73 BamHI G'GATC_C Cuts at: 2953 3320 2953 Size: 367 5256 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2812 2944 4399 618 Size: 325 217 627 1025 132 1455 1842 Fragments arranged by size: 1842 1455 1025 627 325 217 132 BanII G_rGCy'C Cuts at: 1391 2577 2942 1391 Size: 1186 365 4072 Fragments arranged by size: 4072 1186 365 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2351 928 Size: 971 452 4200 Fragments arranged by size: 4200 971 452 BbvCI CC'TCA_GC Cuts at: 2859 2913 2859 Size: 54 5569 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3814 3848 1903 Size: 34 1877 34 3678 Fragments arranged by size: 3678 1877 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3515 4725 3515 Size: 1210 4413 BclI T'GATC_A Cuts at: 1548 1548 Size: 5623 BfrBI ATG'CAT Cuts at: 2788 2788 Size: 5623 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4234 136 Size: 107 122 71 631 605 2562 1525 Fragments arranged by size: 2562 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5618 5618 Size: 5623 BlpI GC'TnA_GC Cuts at: 3040 3040 Size: 5623 Bme1580I G_kGCm'C Cuts at: 1790 2815 3684 4840 1790 Size: 1025 869 1156 2573 Fragments arranged by size: 2573 1156 1025 869 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5623 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3354 4312 442 Size: 416 53 83 399 24 1937 958 1753 Fragments arranged by size: 1937 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2921 1056 Size: 1865 3758 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2193 2572 2753 2800 4283 1239 Size: 954 379 181 47 1483 2579 Fragments arranged by size: 2579 1483 954 379 181 47 Bpu10I CC'TnA_GC Cuts at: 2427 2820 2859 2913 2427 Size: 393 39 54 5137 Fragments arranged by size: 5137 393 54 39 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2007 3748 4613 4911 2007 Size: 1741 865 298 2719 Fragments arranged by size: 2719 1741 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4286 882 Size: 617 2787 2219 Fragments arranged by size: 2787 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3414 493 Size: 711 2210 2702 Fragments arranged by size: 2702 2210 711 BsaBI GATnn'nnATC Cuts at: 5617 5617 Size: 5623 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3430 3812 4447 275 Size: 53 83 186 936 1897 382 635 1451 Fragments arranged by size: 1897 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2370 2682 4056 4454 4728 4875 5286 2370 Size: 312 1374 398 274 147 411 2707 Fragments arranged by size: 2707 1374 411 398 312 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5398 Fragments arranged by size: 5398 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5623 BseYI C'CCAG_C Cuts at: 4826 5322 4826 Size: 496 5127 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5050 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3073 3834 4862 1540 Size: 275 186 1072 761 1028 2301 Fragments arranged by size: 2301 1072 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3684 3769 4840 3684 Size: 85 1071 4467 Fragments arranged by size: 4467 1071 85 BsiWI C'GTAC_G Cuts at: 2644 2644 Size: 5623 BsmI GAATG_Cn' Cuts at: 2790 3134 3227 2790 Size: 344 93 5186 Fragments arranged by size: 5186 344 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2577 2815 2942 3684 3769 4840 1391 Size: 399 787 238 127 742 85 1071 2174 Fragments arranged by size: 2174 1071 787 742 399 238 127 85 BspEI T'CCGG_A Cuts at: 2370 5286 2370 Size: 2916 2707 BspHI T'CATG_A Cuts at: 3512 5242 3512 Size: 1730 3893 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2978 844 Size: 2134 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3510 1394 Size: 429 1687 3507 Fragments arranged by size: 3507 1687 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4118 4292 65 Size: 1183 2870 174 1396 Fragments arranged by size: 2870 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2682 2691 4267 4454 1700 Size: 110 872 9 1576 187 2869 Fragments arranged by size: 2869 1576 872 187 110 9 BsrGI T'GTAC_A Cuts at: 96 2243 96 Size: 2147 3476 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5623 BssSI C'ACGA_G Cuts at: 3683 4695 3683 Size: 1012 4611 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5623 BstBI TT'CG_AA Cuts at: 2748 4427 2748 Size: 1679 3944 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5623 BstXI CCAn_nnnn'nTGG Cuts at: 2236 2236 Size: 5623 BstYI r'GATC_y Cuts at: 1972 2953 3320 3705 3722 5174 5260 5272 5618 Size: 981 367 385 17 1452 86 12 346 Cuts at: 5618 1972 Size: 1977 Fragments arranged by size: 1977 1452 981 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2277 2277 Size: 5623 BtgI C'CryG_G Cuts at: 513 1065 2004 2807 513 Size: 552 939 803 3329 Fragments arranged by size: 3329 939 803 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4884 Fragments arranged by size: 4884 683 56 BtsI GCAGTG_nn' Cuts at: 3139 3933 3953 3139 Size: 794 20 4809 Fragments arranged by size: 4809 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5623 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5588 DraI TTT'AAA Cuts at: 2155 2401 2927 2155 Size: 246 526 4851 Fragments arranged by size: 4851 526 246 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4630 809 Size: 507 3314 1802 Fragments arranged by size: 3314 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2632 2670 2689 Size: 54 1425 50 275 18 802 38 19 Cuts at: 2689 3070 3959 5588 8 Size: 381 889 1629 43 Fragments arranged by size: 1629 1425 889 802 381 275 54 50 43 38 19 18 EagI C'GGCC_G Cuts at: 1537 1812 3070 1537 Size: 275 1258 4090 Fragments arranged by size: 4090 1258 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2880 3553 1353 Size: 1527 673 3423 Fragments arranged by size: 3423 1527 673 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4210 4584 4730 387 Size: 1064 138 2621 374 146 1280 Fragments arranged by size: 2621 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2193 2572 2753 2800 3686 4283 5070 Size: 393 561 379 181 47 886 597 787 Cuts at: 5070 5338 1239 Size: 268 1524 Fragments arranged by size: 1524 886 787 597 561 393 379 268 181 47 EcoRI G'AATT_C Cuts at: 2932 3310 2932 Size: 378 5245 EcoRV GAT'ATC Cuts at: 2434 2434 Size: 5623 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2452 2468 2452 Size: 16 5607 FspI TGC'GCA Cuts at: 4129 4129 Size: 5623 HaeII r_GCGC'y Cuts at: 1678 4770 1678 Size: 3092 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4264 4296 4338 4370 1373 Size: 32 135 32 2692 32 42 32 2626 Fragments arranged by size: 2692 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2760 2967 3213 669 Size: 988 281 822 207 246 3079 Fragments arranged by size: 3079 988 822 281 246 207 HindIII A'AGCT_T Cuts at: 748 748 Size: 5623 HpaI GTT'AAC Cuts at: 3213 3213 Size: 5623 KpnI G_GTAC'C Cuts at: 2948 2948 Size: 5623 MfeI C'AATT_G Cuts at: 3222 3222 Size: 5623 MluI A'CGCG_T Cuts at: 5499 5499 Size: 5623 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4737 4921 1305 Size: 3432 184 2007 Fragments arranged by size: 3432 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2672 10 Size: 54 1425 343 840 2961 Fragments arranged by size: 2961 1425 840 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3581 3940 4099 518 Size: 648 2415 359 159 2042 Fragments arranged by size: 2415 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2249 2550 2863 3039 3716 4864 5109 1606 Size: 643 301 313 176 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 643 313 301 245 176 NcoI C'CATG_G Cuts at: 513 1065 2004 2807 513 Size: 552 939 803 3329 Fragments arranged by size: 3329 939 803 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5623 NheI G'CTAG_C Cuts at: 1052 2917 1052 Size: 1865 3758 NotI GC'GGCC_GC Cuts at: 3070 3070 Size: 5623 NsiI A_TGCA'T Cuts at: 2790 2790 Size: 5623 NspI r_CATG'y Cuts at: 1132 1229 2183 2653 2981 4526 1132 Size: 97 954 470 328 1545 2229 Fragments arranged by size: 2229 1545 954 470 328 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5623 PciI A'CATG_T Cuts at: 4522 4522 Size: 5623 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5623 PfoI T'CCnGG_A Cuts at: 2730 2730 Size: 5623 PmeI GTTT'AAAC Cuts at: 2927 2927 Size: 5623 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3692 3724 5205 5237 3692 Size: 32 1481 32 4078 Fragments arranged by size: 4078 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5623 PsiI TTA'TAA Cuts at: 3193 3193 Size: 5623 PstI C_TGCA'G Cuts at: 830 2975 830 Size: 2145 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5623 PvuII CAG'CTG Cuts at: 2249 2863 2249 Size: 614 5009 RsrII CG'GwC_CG Cuts at: 2221 2221 Size: 5623 SalI G'TCGA_C Cuts at: 1936 2965 1936 Size: 1029 4594 SbfI CC_TGCA'GG Cuts at: 2975 2975 Size: 5623 ScaI AGT'ACT Cuts at: 3871 5583 3871 Size: 1712 3911 SfcI C'TryA_G Cuts at: 826 1045 2097 2971 4106 4787 4978 826 Size: 219 1052 874 1135 681 191 1471 Fragments arranged by size: 1471 1135 1052 874 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5623 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5623 SmaI CCC'GGG Cuts at: 1872 2950 1872 Size: 1078 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2022 2834 3727 4628 4890 820 Size: 197 926 79 812 893 901 262 1553 Fragments arranged by size: 1553 926 901 893 812 262 197 79 SnaBI TAC'GTA Cuts at: 493 3414 493 Size: 2921 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5623 SphI G_CATG'C Cuts at: 1132 2653 2981 1132 Size: 1521 328 3774 Fragments arranged by size: 3774 1521 328 SspI AAT'ATT Cuts at: 5 52 3547 5 Size: 47 3495 2081 Fragments arranged by size: 3495 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5623 StyI C'CwwG_G Cuts at: 513 1065 2004 2490 2721 2807 3062 513 Size: 552 939 486 231 86 255 3074 Fragments arranged by size: 3074 939 552 486 255 231 86 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2493 3650 3667 3820 4005 4450 325 Size: 1255 913 1157 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1157 913 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2243 3869 5581 Size: 274 80 33 51 1443 266 1626 1712 Cuts at: 5581 96 Size: 138 Fragments arranged by size: 1712 1626 1443 274 266 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5583 5615 5583 Size: 32 5591 Tth111I GACn'n_nGTC Cuts at: 1316 2086 1316 Size: 770 4853 XbaI T'CTAG_A Cuts at: 2959 2959 Size: 5623 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2520 2728 2520 Size: 208 5415 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5623 XmaI C'CCGG_G Cuts at: 1870 2948 1870 Size: 1078 4545 XmnI GAAnn'nnTTC Cuts at: 3752 3752 Size: 5623 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3431 276 Size: 53 83 186 936 1897 2468 Fragments arranged by size: 2468 1897 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI