################################## # NO CUT ENZYMES ( 181 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MseI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AfaI 124 GTAC GT^AC AspHI 282 GTGCTC G[AT]GC[AT]^C AvaI 62 CCCGGG C^[CT]CG[GA]G AvaII 157 GGTCC G^G[AT]CC BamHI 52 GGATCC G^GATCC BfaI 200 CTAG C^TAG BmyI 282 GTGCTC G[AGT]GC[ACT]^C BsaI 6 GGTCTC GGTCTC(1/5) BseRI 144 GAGGAG GAGGAG(10/8) BsiHKAI 282 GTGCTC G[AT]GC[AT]^C BsmI 371 GAATGC GAATGC(1/-1) BsoBI 62 CCCGGG C^[CT]CG[GA]G Bsp1286I 282 GTGCTC G[AGT]GC[ACT]^C BspHI 92 TCATGA T^CATGA BstF5I 237 CATCC GGATG(2/0) BstYI 52 GGATCC [GA]^GATC[CT] DdeI 161 CTCAG C^T[ACGT]AG DsaI 11 CCGTGG C^C[GA][CT]GG EcoT14I 48 CCTTGG C^C[AT][AT]GG FokI 237 CATCC GGATG(9/13) HaeIII 60 GGCC GG^CC HindIII 103 AAGCTT A^AGCTT HinfI 188 GATTC G^A[ACGT]TC HphI 308 TCACC GGTGA(8/7) MaeI 200 CTAG C^TAG MaeII 324 ACGT A^CGT MflI 52 GGATCC [GA]^GATC[CT] MwoI 196 GCTACTAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 52 GGATCC GG[ACGT]^[ACGT]CC NspI 134 ACATGC [GA]CATG^[CT] PstI 182 CTGCAG CTGCA^G RcaI 92 TCATGA T^CATGA RsaI 124 GTAC GT^AC ScaI 123 AGTACT AGT^ACT SfcI 182 CTGCAG C^T[GA][CT]AG SmaI 62 CCCGGG CCC^GGG SpeI 199 ACTAGT A^CTAGT StyI 48 CCTTGG C^C[AT][AT]GG TfiI 188 GATTC G^A[AT]TC Tsp45I 67 GTCAC ^GT[GC]AC TspRI 342 CAGTG CA[GC]TG[ACGT][ACGT]^ XhoII 52 GGATCC [GA]^GATC[CT] XmaCI 62 CCCGGG C^CCGGG XmaI 62 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AluI 104 AGCT AG^CT 380 AGCT BpmI 141 CTGGAG CTGGAG(16/14) 253 CTGGAG BsmAI 82 GAGAC GTCTC(1/5) 7 GTCTC BsrI 121 CCAGT ACTGG(1/-1) 341 CCAGT Cfr13I 60 GGCCC G^G[ACGT]CC 157 GGTCC DpnII 53 GATC ^GATC 393 GATC HapII 63 CCGG C^CGG 292 CCGG HpaII 63 CCGG C^CGG 292 CCGG MboI 53 GATC ^GATC 393 GATC MboII 405 GAAGA GAAGA(8/7) 302 TCTTC MspI 63 CCGG C^CGG 292 CCGG NdeII 53 GATC ^GATC 393 GATC Sau3AI 53 GATC ^GATC 393 GATC Sau96I 60 GGCCC G^G[ACGT]CC 157 GGTCC ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AlwI BbvI BcnI BsaJI BsiYI BslI BssKI BstNI EcoRII Fnu4HI ItaI MaeIII MnlI MvaI NciI NlaIII ScrFI SfaNI TseI