################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII BamHI BanI BbeI BbrPI BbvCI BcgI BfrI BglI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaI KasI KpnI Ksp632I KspI MamI MluI MroI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AcsI 1168 AAATTT [GA]^AATT[CT] AflIII 581 ACATGT A^C[GA][CT]GT AlwNI 1028 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 1168 AAATTT [GA]^AATT[CT] AvrII 1038 CCTAGG C^CTAGG BalI 815 TGGCCA TGG^CCA BbsI 253 GTCTTC GAAGAC(2/6) BciVI 1328 GTATCC GTATCC(6/5) BclI 1370 TGATCA T^GATCA BcnI 369 CCCGG CC^[GC]GG BglII 53 AGATCT A^GATCT BlnI 1038 CCTAGG C^CTAGG BpuAI 253 GTCTTC GAAGAC(2/6) BsaI 934 GAGACC GGTCTC(1/5) BsaWI 290 TCCGGT [AT]^CCGG[AT] BspLU11I 581 ACATGT A^CATGT BsrI 20 CCAGT ACTGG(1/-1) BstAPI 1027 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC EcoNI 1033 CCTGCCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 1370 TGATCA T^GATCA HincII 355 GTCAAC GT[CT]^[GA]AC HindII 355 GTCAAC GT[CT]^[GA]AC MaeII 1490 ACGT A^CGT MfeI 1247 CAATTG C^AATTG MluNI 815 TGGCCA TGG^CCA MscI 815 TGGCCA TGG^CCA MunI 1247 CAATTG C^AATTG NciI 369 CCCGG CC^[GC]GG PflMI 771 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1513 GGGACCT [GA]G^G[AT]CC[CT] PvuII 260 CAGCTG CAG^CTG ScaI 1438 AGTACT AGT^ACT SphI 455 GCATGC GCATG^C TaqI 887 TCGA T^CGA TthHB8I 887 TCGA T^CGA Van91I 771 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BseRI 1405 GAGGAG GAGGAG(10/8) 1522 GAGGAG BsgI 469 CTGCAC GTGCAG(16/14) 1025 GTGCAG BsmFI 634 GGGAC GGGAC(10/14) 1513 GGGAC DraII 404 AGGCCCT [GA]G^G[ACGT]CC[CT] 1513 GGGACCT DraIII 472 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG 1480 CACACTGTG EaeI 534 CGGCCA [CT]^GGCC[GA] 815 TGGCCA EcoO109I 404 AGGCCCT [GA]G^G[ACGT]CC[CT] 1513 GGGACCT FauI 938 CCCGC CCCGC(4/6) 1100 CCCGC MseI 587 TTAA T^TAA 1172 TTAA MspA1I 109 CCGCTG C[AC]G^C[GT]G 260 CAGCTG NcoI 78 CCATGG C^CATGG 1121 CCATGG SmlI 7 CTCAAG C^T[CT][GA]AG 1159 CTTGAG Tru9I 587 TTAA T^TAA 1172 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaII BanII BbvI BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DsaI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MslI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I Tsp509I TspRI XhoII