(Circular) (Six-base) MAPSORT of: pFN21AE5621.seq Check: 1379 from: 1 to: 6241 pFN21AE5621 6241 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4051 278 Size: 53 83 186 936 2515 2468 Fragments arranged by size: 2515 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3584 1937 Size: 1647 4594 Acc65I G'GTAC_C Cuts at: 3562 3562 Size: 6241 AclI AA'CG_TT Cuts at: 1563 4368 4741 1563 Size: 2805 373 3063 Fragments arranged by size: 3063 2805 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2516 4304 5688 5956 1632 Size: 884 1788 1384 268 1917 Fragments arranged by size: 1917 1788 1384 884 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6044 AflIII A'CryG_T Cuts at: 1203 2586 5140 6117 1203 Size: 1383 2554 977 1327 Fragments arranged by size: 2554 1383 1327 977 AgeI A'CCGG_T Cuts at: 5072 5072 Size: 6241 AhdI GACnn_n'nnGTC Cuts at: 4970 4970 Size: 6241 AleI CACnn'nnGTG Cuts at: 1166 2228 2558 1166 Size: 1062 330 4849 Fragments arranged by size: 4849 1062 330 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2633 1184 Size: 1449 4792 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2724 2756 2724 Size: 32 6209 AlwNI CAG_nnn'CTG Cuts at: 3038 3984 5556 3038 Size: 946 1572 3723 Fragments arranged by size: 3723 1572 946 ApaLI G'TGCA_C Cuts at: 4298 5454 4298 Size: 1156 5085 ApoI r'AATT_y Cuts at: 1493 3173 3550 3776 3928 6037 1493 Size: 1680 377 226 152 2109 1697 Fragments arranged by size: 2109 1697 1680 377 226 152 AseI AT'TA_AT Cuts at: 160 1032 4795 160 Size: 872 3763 1606 Fragments arranged by size: 3763 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6241 AvaI C'yCGr_G Cuts at: 1870 1943 3566 1870 Size: 73 1623 4545 Fragments arranged by size: 4545 1623 73 AvrII C'CTAG_G Cuts at: 3043 3043 Size: 6241 BamHI G'GATC_C Cuts at: 3571 3938 3571 Size: 367 5874 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3562 5017 618 Size: 325 217 627 1775 1455 1842 Fragments arranged by size: 1842 1775 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2159 2186 3123 3354 3560 1391 Size: 768 27 937 231 206 4072 Fragments arranged by size: 4072 937 768 231 206 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2251 928 Size: 971 352 4918 Fragments arranged by size: 4918 971 352 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4432 4466 1903 Size: 34 2495 34 3678 Fragments arranged by size: 3678 2495 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3344 4133 5343 3344 Size: 789 1210 4242 Fragments arranged by size: 4242 1210 789 BclI T'GATC_A Cuts at: 1548 3375 1548 Size: 1827 4414 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4852 136 Size: 107 122 71 631 605 3180 1525 Fragments arranged by size: 3180 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2058 6236 2058 Size: 4178 2063 BlpI GC'TnA_GC Cuts at: 3658 3658 Size: 6241 Bme1580I G_kGCm'C Cuts at: 1790 3501 4302 5458 1790 Size: 1711 801 1156 2573 Fragments arranged by size: 2573 1711 1156 801 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6241 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2019 3972 4930 Size: 416 53 83 399 24 602 1953 958 Cuts at: 4930 442 Size: 1753 Fragments arranged by size: 1953 1753 958 602 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6241 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2287 2426 2486 2675 2784 4901 1239 Size: 1048 139 60 189 109 2117 2579 Fragments arranged by size: 2579 2117 1048 189 139 109 60 Bpu10I CC'TnA_GC Cuts at: 3012 3012 Size: 6241 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1997 3185 4366 5231 5529 1997 Size: 1188 1181 865 298 2709 Fragments arranged by size: 2709 1188 1181 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2933 4904 882 Size: 617 1434 1971 2219 Fragments arranged by size: 2219 1971 1434 617 BsaAI yAC'GTr Cuts at: 493 1204 4032 493 Size: 711 2828 2702 Fragments arranged by size: 2828 2702 711 BsaBI GATnn'nnATC Cuts at: 6235 6235 Size: 6241 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4048 4430 5065 275 Size: 53 83 186 936 2515 382 635 1451 Fragments arranged by size: 2515 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2295 4674 5072 5346 5493 5904 2295 Size: 2379 398 274 147 411 2632 Fragments arranged by size: 2632 2379 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2192 2222 2724 2754 1178 Size: 30 165 30 789 30 502 30 4665 Fragments arranged by size: 4665 789 502 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3425 3542 1186 Size: 2239 117 3885 Fragments arranged by size: 3885 2239 117 BseYI C'CCAG_C Cuts at: 2263 2333 2782 3140 5444 5940 2263 Size: 70 449 358 2304 496 2564 Fragments arranged by size: 2564 2304 496 449 358 70 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2459 3051 1163 Size: 573 723 592 4353 Fragments arranged by size: 4353 723 592 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3691 4452 5480 1540 Size: 275 186 1690 761 1028 2301 Fragments arranged by size: 2301 1690 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2186 3123 3354 4302 4387 5458 2186 Size: 937 231 948 85 1071 2969 Fragments arranged by size: 2969 1071 948 937 231 85 BsmI GAATG_Cn' Cuts at: 3752 3845 3752 Size: 93 6148 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2159 2186 3123 3354 3501 3560 4302 Size: 399 369 27 937 231 147 59 742 Cuts at: 4302 4387 5458 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 937 742 399 369 231 147 85 59 27 BspEI T'CCGG_A Cuts at: 5904 5904 Size: 6241 BspHI T'CATG_A Cuts at: 4130 5860 4130 Size: 1730 4511 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2373 2697 2856 3405 3596 844 Size: 1529 324 159 549 191 3489 Fragments arranged by size: 3489 1529 549 324 191 159 BsrBI CCG'CTC Cuts at: 1394 1823 4128 1394 Size: 429 2305 3507 Fragments arranged by size: 3507 2305 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4736 4910 65 Size: 1183 3488 174 1396 Fragments arranged by size: 3488 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4885 5072 1700 Size: 110 3075 187 2869 Fragments arranged by size: 3075 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6241 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6241 BssSI C'ACGA_G Cuts at: 4301 5313 4301 Size: 1012 5229 BstAPI GCAn_nnn'nTGC Cuts at: 794 3038 794 Size: 2244 3997 BstBI TT'CG_AA Cuts at: 5045 5045 Size: 6241 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6241 BstYI r'GATC_y Cuts at: 1972 2058 2370 2745 3135 3571 3938 4323 4340 Size: 86 312 375 390 436 367 385 17 Cuts at: 4340 5792 5878 5890 6236 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 436 390 385 375 367 346 312 86 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2083 2625 2858 3126 513 Size: 552 939 79 542 233 268 3628 Fragments arranged by size: 3628 939 552 542 268 233 79 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5502 Fragments arranged by size: 5502 683 56 BtsI GCAGTG_nn' Cuts at: 2148 3757 4551 4571 2148 Size: 1609 794 20 3818 Fragments arranged by size: 3818 1609 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6241 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6206 DraI TTT'AAA Cuts at: 3545 3545 Size: 6241 DraIII CAC_nnn'GTG Cuts at: 2482 3490 2482 Size: 1008 5233 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5248 809 Size: 507 3932 1802 Fragments arranged by size: 3932 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2539 2820 3688 Size: 54 1425 50 275 18 709 281 868 Cuts at: 3688 4577 6206 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 868 709 281 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3688 1537 Size: 275 1876 4090 Fragments arranged by size: 4090 1876 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4171 1353 Size: 2818 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2081 3071 4828 5202 5348 387 Size: 1064 138 492 990 1757 374 146 1280 Fragments arranged by size: 1757 1280 1064 990 492 374 146 138 EcoICRI GAG'CTC Cuts at: 2184 3121 3352 2184 Size: 937 231 5073 Fragments arranged by size: 5073 937 231 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2287 2426 2486 2516 2675 2784 4304 Size: 393 655 139 60 30 159 109 1520 Cuts at: 4304 4901 5688 5956 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1520 787 655 597 393 268 159 139 109 60 30 EcoNI CCTnn'n_nnAGG Cuts at: 3042 3042 Size: 6241 EcoO109I rG'GnC_Cy Cuts at: 2410 3519 2410 Size: 1109 5132 EcoRI G'AATT_C Cuts at: 3550 3928 3550 Size: 378 5863 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2446 2446 Size: 6241 FspI TGC'GCA Cuts at: 4747 4747 Size: 6241 HaeII r_GCGC'y Cuts at: 1678 5388 1678 Size: 3710 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2180 2212 2737 2769 2858 Size: 32 135 32 608 32 525 32 89 Cuts at: 2858 2890 4882 4914 4956 4988 1373 Size: 32 1992 32 42 32 2626 Fragments arranged by size: 2626 1992 608 525 135 89 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2362 3585 3831 669 Size: 988 281 424 1223 246 3079 Fragments arranged by size: 3079 1223 988 424 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6241 HpaI GTT'AAC Cuts at: 3831 3831 Size: 6241 KpnI G_GTAC'C Cuts at: 3566 3566 Size: 6241 MfeI C'AATT_G Cuts at: 3252 3840 3252 Size: 588 5653 MluI A'CGCG_T Cuts at: 6117 6117 Size: 6241 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5355 5539 1305 Size: 4050 184 2007 Fragments arranged by size: 4050 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2822 10 Size: 54 1425 343 990 3429 Fragments arranged by size: 3429 1425 990 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2102 2228 2558 4199 4558 4717 518 Size: 648 936 126 330 1641 359 159 2042 Fragments arranged by size: 2042 1641 936 648 359 330 159 126 MspA1I CmG'CkG Cuts at: 1606 2116 2267 3657 4334 5482 5727 1606 Size: 510 151 1390 677 1148 245 2120 Fragments arranged by size: 2120 1390 1148 677 510 245 151 NcoI C'CATG_G Cuts at: 513 1065 2004 2083 3126 513 Size: 552 939 79 1043 3628 Fragments arranged by size: 3628 1043 939 552 79 NdeI CA'TA_TG Cuts at: 387 387 Size: 6241 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6241 NotI GC'GGCC_GC Cuts at: 3688 3688 Size: 6241 NspI r_CATG'y Cuts at: 1132 1229 2222 2464 2590 2986 3599 5144 1132 Size: 97 993 242 126 396 613 1545 2229 Fragments arranged by size: 2229 1545 993 613 396 242 126 97 PasI CC'CwG_GG Cuts at: 1348 3514 1348 Size: 2166 4075 PciI A'CATG_T Cuts at: 2586 5140 2586 Size: 2554 3687 PflMI CCAn_nnn'nTGG Cuts at: 1108 2782 1108 Size: 1674 4567 PfoI T'CCnGG_A Cuts at: 2373 2373 Size: 6241 PmeI GTTT'AAAC Cuts at: 3545 3545 Size: 6241 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2724 2756 4310 4342 5823 5855 2724 Size: 32 1554 32 1481 32 3110 Fragments arranged by size: 3110 1554 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3519 3519 Size: 6241 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6241 PsiI TTA'TAA Cuts at: 3811 3811 Size: 6241 PstI C_TGCA'G Cuts at: 830 2370 3086 3507 3593 830 Size: 1540 716 421 86 3478 Fragments arranged by size: 3478 1540 716 421 86 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6241 PvuII CAG'CTG Cuts at: 2267 2267 Size: 6241 SacI G_AGCT'C Cuts at: 2186 3123 3354 2186 Size: 937 231 5073 Fragments arranged by size: 5073 937 231 SalI G'TCGA_C Cuts at: 1936 3583 1936 Size: 1647 4594 SbfI CC_TGCA'GG Cuts at: 3593 3593 Size: 6241 ScaI AGT'ACT Cuts at: 3445 4489 6201 3445 Size: 1044 1712 3485 Fragments arranged by size: 3485 1712 1044 SfcI C'TryA_G Cuts at: 826 1045 2366 2923 3082 3503 3589 4724 5405 Size: 219 1321 557 159 421 86 1135 681 Cuts at: 5405 5596 826 Size: 191 1471 Fragments arranged by size: 1471 1321 1135 681 557 421 219 191 159 86 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6241 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6241 SmaI CCC'GGG Cuts at: 1872 3568 1872 Size: 1696 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2012 3164 4345 5246 5508 820 Size: 197 926 69 1152 1181 901 262 1553 Fragments arranged by size: 1553 1181 1152 926 901 262 197 69 SnaBI TAC'GTA Cuts at: 493 4032 493 Size: 3539 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6241 SphI G_CATG'C Cuts at: 1132 2464 3599 1132 Size: 1332 1135 3774 Fragments arranged by size: 3774 1332 1135 SspI AAT'ATT Cuts at: 5 52 4165 5 Size: 47 4113 2081 Fragments arranged by size: 4113 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6241 StyI C'CwwG_G Cuts at: 513 1065 2004 2083 3043 3126 3522 3680 513 Size: 552 939 79 960 83 396 158 3074 Fragments arranged by size: 3074 960 939 552 396 158 83 79 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4268 4285 4438 4623 5068 325 Size: 1255 2688 17 153 185 445 1498 Fragments arranged by size: 2688 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3443 4487 6199 Size: 274 80 33 51 1443 1466 1044 1712 Cuts at: 6199 96 Size: 138 Fragments arranged by size: 1712 1466 1443 1044 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2757 2789 2920 2952 6201 6233 2757 Size: 32 131 32 3249 32 2765 Fragments arranged by size: 3249 2765 131 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6241 XbaI T'CTAG_A Cuts at: 3577 3577 Size: 6241 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6241 XmaI C'CCGG_G Cuts at: 1870 3566 1870 Size: 1696 4545 XmnI GAAnn'nnTTC Cuts at: 4370 4370 Size: 6241 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4049 276 Size: 53 83 186 936 2515 2468 Fragments arranged by size: 2515 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI