(Circular) (Six-base) MAPSORT of: pFN21AE2551.seq Check: 2841 from: 1 to: 6595 pFN21AE2551 6595 bp With 182 enzymes: SgfI * August 28, 2015 12:06 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3428 3428 Size: 6595 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4405 278 Size: 53 83 186 936 2869 2468 Fragments arranged by size: 2869 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3938 1937 Size: 2001 4594 Acc65I G'GTAC_C Cuts at: 3916 3916 Size: 6595 AclI AA'CG_TT Cuts at: 1563 4722 5095 1563 Size: 3159 373 3063 Fragments arranged by size: 3159 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2709 2791 3580 4658 6042 6310 1632 Size: 1077 82 789 1078 1384 268 1917 Fragments arranged by size: 1917 1384 1078 1077 789 268 82 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6398 AflIII A'CryG_T Cuts at: 1203 3370 5494 6471 1203 Size: 2167 2124 977 1327 Fragments arranged by size: 2167 2124 1327 977 AgeI A'CCGG_T Cuts at: 2724 5426 2724 Size: 2702 3893 AhdI GACnn_n'nnGTC Cuts at: 5324 5324 Size: 6595 AleI CACnn'nnGTG Cuts at: 1166 2213 3350 1166 Size: 1047 1137 4411 Fragments arranged by size: 4411 1137 1047 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6595 AlwNI CAG_nnn'CTG Cuts at: 2746 2814 3536 4338 5910 2746 Size: 68 722 802 1572 3431 Fragments arranged by size: 3431 1572 802 722 68 ApaI G_GGCC'C Cuts at: 3501 3501 Size: 6595 ApaLI G'TGCA_C Cuts at: 4652 5808 4652 Size: 1156 5439 ApoI r'AATT_y Cuts at: 1493 2023 2693 3861 3904 4130 4282 6391 1493 Size: 530 670 1168 43 226 152 2109 1697 Fragments arranged by size: 2109 1697 1168 670 530 226 152 43 AseI AT'TA_AT Cuts at: 160 1032 5149 160 Size: 872 4117 1606 Fragments arranged by size: 4117 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6595 AvaI C'yCGr_G Cuts at: 1870 1943 3601 3920 1870 Size: 73 1658 319 4545 Fragments arranged by size: 4545 1658 319 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3709 3742 3709 Size: 33 6562 BamHI G'GATC_C Cuts at: 3431 3925 4292 3431 Size: 494 367 5734 Fragments arranged by size: 5734 494 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2900 3916 5371 618 Size: 325 217 627 1113 1016 1455 1842 Fragments arranged by size: 1842 1455 1113 1016 627 325 217 BanII G_rGCy'C Cuts at: 1391 2415 2739 3501 3683 3914 1391 Size: 1024 324 762 182 231 4072 Fragments arranged by size: 4072 1024 762 324 231 182 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5624 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2424 2458 4786 4820 1903 Size: 34 487 34 2328 34 3678 Fragments arranged by size: 3678 2328 487 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4487 5697 4487 Size: 1210 5385 BclI T'GATC_A Cuts at: 1548 3342 3795 1548 Size: 1794 453 4348 Fragments arranged by size: 4348 1794 453 BfrBI ATG'CAT Cuts at: 2445 2445 Size: 6595 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5206 136 Size: 107 122 71 631 605 3534 1525 Fragments arranged by size: 3534 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3000 6590 3000 Size: 3590 3005 BlpI GC'TnA_GC Cuts at: 2220 4012 2220 Size: 1792 4803 Bme1580I G_kGCm'C Cuts at: 1790 3501 4656 5812 1790 Size: 1711 1155 1156 2573 Fragments arranged by size: 2573 1711 1156 1155 BmgBI CAC'GTC Cuts at: 1318 2679 2710 3373 3577 1318 Size: 1361 31 663 204 4336 Fragments arranged by size: 4336 1361 663 204 31 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2429 4326 5284 Size: 416 53 83 399 24 1012 1897 958 Cuts at: 5284 442 Size: 1753 Fragments arranged by size: 1897 1753 1012 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3051 1056 Size: 1995 4600 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2723 2934 2941 2987 3500 3634 3742 3789 Size: 1484 211 7 46 513 134 108 47 Cuts at: 3789 5255 1239 Size: 1466 2579 Fragments arranged by size: 2579 1484 1466 513 211 134 108 47 46 7 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4720 5585 5883 4720 Size: 865 298 5432 Fragments arranged by size: 5432 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2237 5258 882 Size: 617 738 3021 2219 Fragments arranged by size: 3021 2219 738 617 BsaAI yAC'GTr Cuts at: 493 1204 3231 3466 4386 493 Size: 711 2027 235 920 2702 Fragments arranged by size: 2702 2027 920 711 235 BsaBI GATnn'nnATC Cuts at: 3296 6589 3296 Size: 3293 3302 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2015 2823 4402 4784 Size: 53 83 186 936 482 808 1579 382 Cuts at: 4784 5419 275 Size: 635 1451 Fragments arranged by size: 1579 1451 936 808 635 482 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2724 5028 5426 5700 5847 6258 2724 Size: 2304 398 274 147 411 3061 Fragments arranged by size: 3061 2304 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2552 2582 1178 Size: 30 165 30 1149 30 5191 Fragments arranged by size: 5191 1149 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2348 2560 3209 3497 1186 Size: 1162 212 649 288 4284 Fragments arranged by size: 4284 1162 649 288 212 BseYI C'CCAG_C Cuts at: 2194 3212 3304 3624 5798 6294 2194 Size: 1018 92 320 2174 496 2495 Fragments arranged by size: 2495 2174 1018 496 320 92 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2614 1163 Size: 573 878 5144 Fragments arranged by size: 5144 878 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4045 4806 5834 1540 Size: 275 186 2044 761 1028 2301 Fragments arranged by size: 2301 2044 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2415 3153 3405 3510 4656 4741 5812 2415 Size: 738 252 105 1146 85 1071 3198 Fragments arranged by size: 3198 1146 1071 738 252 105 85 BsmI GAATG_Cn' Cuts at: 2351 3892 4106 4199 2351 Size: 1541 214 93 4747 Fragments arranged by size: 4747 1541 214 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2003 2003 Size: 6595 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2415 2739 3153 3405 3501 3510 3683 Size: 399 625 324 414 252 96 9 173 Cuts at: 3683 3914 4656 4741 5812 1391 Size: 231 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 625 414 399 324 252 231 173 96 85 9 BspEI T'CCGG_A Cuts at: 6258 6258 Size: 6595 BspHI T'CATG_A Cuts at: 4484 6214 4484 Size: 1730 4865 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3197 3428 3950 844 Size: 2353 231 522 3489 Fragments arranged by size: 3489 2353 522 231 BsrBI CCG'CTC Cuts at: 1394 1823 2398 2996 3394 4482 1394 Size: 429 575 598 398 1088 3507 Fragments arranged by size: 3507 1088 598 575 429 398 BsrDI GCAATG_nn' Cuts at: 65 1248 2311 2505 2517 5090 5264 65 Size: 1183 1063 194 12 2573 174 1396 Fragments arranged by size: 2573 1396 1183 1063 194 174 12 BsrFI r'CCGG_y Cuts at: 1700 1810 2724 5239 5426 1700 Size: 110 914 2515 187 2869 Fragments arranged by size: 2869 2515 914 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6595 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6595 BssSI C'ACGA_G Cuts at: 4655 5667 4655 Size: 1012 5583 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6595 BstBI TT'CG_AA Cuts at: 5399 5399 Size: 6595 BstEII G'GTnAC_C Cuts at: 1184 3091 3439 1184 Size: 1907 348 4340 Fragments arranged by size: 4340 1907 348 BstXI CCAn_nnnn'nTGG Cuts at: 2215 2438 3375 2215 Size: 223 937 5435 Fragments arranged by size: 5435 937 223 BstYI r'GATC_y Cuts at: 1972 2718 3000 3431 3618 3925 4292 4677 4694 Size: 746 282 431 187 307 367 385 17 Cuts at: 4694 6146 6232 6244 6590 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 746 431 385 367 346 307 282 187 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3408 3492 513 Size: 552 939 1404 84 3616 Fragments arranged by size: 3616 1404 939 552 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5856 Fragments arranged by size: 5856 683 56 BtsI GCAGTG_nn' Cuts at: 3510 4111 4905 4925 3510 Size: 601 794 20 5180 Fragments arranged by size: 5180 794 601 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6595 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3253 3288 529 Size: 35 2689 35 3836 Fragments arranged by size: 3836 2689 35 35 DraI TTT'AAA Cuts at: 3817 3899 3817 Size: 82 6513 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5602 809 Size: 507 4286 1802 Fragments arranged by size: 4286 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2376 3195 3630 Size: 54 1425 50 275 18 546 819 435 Cuts at: 3630 4042 4931 6560 8 Size: 412 889 1629 43 Fragments arranged by size: 1629 1425 889 819 546 435 412 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4042 1537 Size: 275 2230 4090 Fragments arranged by size: 4090 2230 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2939 3227 4525 1353 Size: 1586 288 1298 3423 Fragments arranged by size: 3423 1586 1298 288 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5182 5556 5702 387 Size: 1064 138 3593 374 146 1280 Fragments arranged by size: 3593 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2413 2413 Size: 6595 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2709 2723 2791 2934 2941 2987 3500 Size: 393 1077 14 68 143 7 46 513 Cuts at: 3500 3580 3634 3742 3789 4658 5255 6042 6310 Size: 80 54 108 47 869 597 787 268 Cuts at: 6310 1239 Size: 1524 Fragments arranged by size: 1524 1077 869 787 597 513 393 268 143 108 80 68 54 47 46 14 7 EcoO109I rG'GnC_Cy Cuts at: 2743 3031 3209 3459 3497 2743 Size: 288 178 250 38 5841 Fragments arranged by size: 5841 288 250 178 38 EcoRI G'AATT_C Cuts at: 2023 3904 4282 2023 Size: 1881 378 4336 Fragments arranged by size: 4336 1881 378 FspI TGC'GCA Cuts at: 5101 5101 Size: 6595 HaeII r_GCGC'y Cuts at: 1678 2317 2555 3388 5742 1678 Size: 639 238 833 2354 2531 Fragments arranged by size: 2531 2354 833 639 238 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3123 3155 3423 3455 3787 Size: 32 135 32 1551 32 268 32 332 Cuts at: 3787 3819 5236 5268 5310 5342 1373 Size: 32 1417 32 42 32 2626 Fragments arranged by size: 2626 1551 1417 332 268 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3939 4185 669 Size: 988 281 2001 246 3079 Fragments arranged by size: 3079 2001 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6595 HpaI GTT'AAC Cuts at: 4185 4185 Size: 6595 KpnI G_GTAC'C Cuts at: 3920 3920 Size: 6595 MfeI C'AATT_G Cuts at: 2950 4194 2950 Size: 1244 5351 MluI A'CGCG_T Cuts at: 6471 6471 Size: 6595 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5709 5893 1305 Size: 4404 184 2007 Fragments arranged by size: 4404 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2378 3197 3632 10 Size: 54 1425 343 546 819 435 2973 Fragments arranged by size: 2973 1425 819 546 435 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2213 2372 2436 2540 3350 4553 4912 Size: 648 1047 159 64 104 810 1203 359 Cuts at: 4912 5071 518 Size: 159 2042 Fragments arranged by size: 2042 1203 1047 810 648 359 159 159 104 64 MspA1I CmG'CkG Cuts at: 1606 2587 2601 2760 2767 4011 4688 5836 6081 Size: 981 14 159 7 1244 677 1148 245 Cuts at: 6081 1606 Size: 2120 Fragments arranged by size: 2120 1244 1148 981 677 245 159 14 7 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5104 Fragments arranged by size: 5104 939 552 NdeI CA'TA_TG Cuts at: 387 2327 387 Size: 1940 4655 NheI G'CTAG_C Cuts at: 1052 3047 1052 Size: 1995 4600 NotI GC'GGCC_GC Cuts at: 4042 4042 Size: 6595 NsiI A_TGCA'T Cuts at: 2447 2447 Size: 6595 NspI r_CATG'y Cuts at: 1132 1229 3256 3953 5498 1132 Size: 97 2027 697 1545 2229 Fragments arranged by size: 2229 2027 1545 697 97 PasI CC'CwG_GG Cuts at: 1348 2105 1348 Size: 757 5838 PciI A'CATG_T Cuts at: 5494 5494 Size: 6595 PflMI CCAn_nnn'nTGG Cuts at: 1108 2162 2746 1108 Size: 1054 584 4957 Fragments arranged by size: 4957 1054 584 PfoI T'CCnGG_A Cuts at: 2612 2739 2964 2612 Size: 127 225 6243 Fragments arranged by size: 6243 225 127 PmeI GTTT'AAAC Cuts at: 3899 3899 Size: 6595 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4664 4696 6177 6209 4664 Size: 32 1481 32 5050 Fragments arranged by size: 5050 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2743 3031 3209 2743 Size: 288 178 6129 Fragments arranged by size: 6129 288 178 PshAI GACnn'nnGTC Cuts at: 1613 3207 1613 Size: 1594 5001 PsiI TTA'TAA Cuts at: 4165 4165 Size: 6595 PspOMI G'GGCC_C Cuts at: 3497 3497 Size: 6595 PstI C_TGCA'G Cuts at: 830 2718 3572 3947 830 Size: 1888 854 375 3478 Fragments arranged by size: 3478 1888 854 375 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6595 PvuII CAG'CTG Cuts at: 2587 2601 2760 2767 2587 Size: 14 159 7 6415 Fragments arranged by size: 6415 159 14 7 SacI G_AGCT'C Cuts at: 2415 2415 Size: 6595 SalI G'TCGA_C Cuts at: 1936 3937 1936 Size: 2001 4594 SbfI CC_TGCA'GG Cuts at: 3947 3947 Size: 6595 ScaI AGT'ACT Cuts at: 4843 6555 4843 Size: 1712 4883 SfcI C'TryA_G Cuts at: 826 1045 2084 2714 3568 3943 5078 5759 5950 Size: 219 1039 630 854 375 1135 681 191 Cuts at: 5950 826 Size: 1471 Fragments arranged by size: 1471 1135 1039 854 681 630 375 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6595 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6595 SmaI CCC'GGG Cuts at: 1872 3603 3922 1872 Size: 1731 319 4545 Fragments arranged by size: 4545 1731 319 SmlI C'TyrA_G Cuts at: 820 1017 1943 4699 5600 5862 820 Size: 197 926 2756 901 262 1553 Fragments arranged by size: 2756 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4386 493 Size: 3893 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6595 SphI G_CATG'C Cuts at: 1132 3953 1132 Size: 2821 3774 SspI AAT'ATT Cuts at: 5 52 4519 5 Size: 47 4467 2081 Fragments arranged by size: 4467 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6595 StyI C'CwwG_G Cuts at: 513 1065 2004 4034 513 Size: 552 939 2030 3074 Fragments arranged by size: 3074 2030 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2839 3616 4622 4639 4792 4977 5422 Size: 1255 1259 777 1006 17 153 185 445 Cuts at: 5422 325 Size: 1498 Fragments arranged by size: 1498 1259 1255 1006 777 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4841 6553 96 Size: 274 80 33 51 1443 2864 1712 138 Fragments arranged by size: 2864 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6555 6587 6555 Size: 32 6563 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6595 XbaI T'CTAG_A Cuts at: 3931 3931 Size: 6595 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2212 2212 Size: 6595 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6595 XmaI C'CCGG_G Cuts at: 1870 3601 3920 1870 Size: 1731 319 4545 Fragments arranged by size: 4545 1731 319 XmnI GAAnn'nnTTC Cuts at: 4724 4724 Size: 6595 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4403 276 Size: 53 83 186 936 2869 2468 Fragments arranged by size: 2869 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI