################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglI BlnI BpuAI BsaAI BseAI BsgI BsiEI BsiHKAI BsiWI BsmI BsoBI Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FauI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MaeII MfeI MluI MluNI MroI MscI MseI MspA1I MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AciI 339 CCGC CCGC(-3/-1) AlwI 618 GATCC GGATC(4/5) Asp700I 31 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 211 GGCGCC G^G[CT][GA]CC BanII 594 GGGCTC G[GA]GC[CT]^C BbeI 211 GGCGCC GGCGC^C BbvI 18 GCAGC GCAGC(8/12) BclI 108 TGATCA T^GATCA BcnI 73 CCGGG CC^[GC]GG BglII 419 AGATCT A^GATCT BlpI 13 GCTGAGC GC^T[ACGT]AGC BmyI 594 GGGCTC G[AGT]GC[ACT]^C Bpu1102I 13 GCTGAGC GC^T[ACGT]AGC BsaI 4 GAGACC GGTCTC(1/5) BsaWI 188 TCCGGT [AT]^CCGG[AT] BsmBI 325 CGTCTC CGTCTC(1/5) Bsp1286I 594 GGGCTC G[AGT]GC[ACT]^C BspDI 624 ATCGAT AT^CGAT BsrI 411 CCAGT ACTGG(1/-1) Cac8I 444 GCAAGC GC[ACGT]^[ACGT]GC CelII 13 GCTGAGC GC^T[ACGT]AGC ClaI 624 ATCGAT AT^CGAT EcoRV 524 GATATC GAT^ATC FbaI 108 TGATCA T^GATCA Fnu4HI 18 GCAGC GC^[ACGT]GC HaeII 211 GGCGCC [GA]GCGC^[CT] HphI 143 TCACC GGTGA(8/7) ItaI 18 GCAGC GC^[ACGT]GC KasI 211 GGCGCC G^GCGCC MaeIII 554 GTGAC ^GT[ACGT]AC MslI 45 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MwoI 18 GCAGCAGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NarI 211 GGCGCC GG^CGCC NciI 73 CCGGG CC^[GC]GG PflMI 449 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 367 GAGTC GAGTC(4/5) PpuMI 76 GGGTCCC [GA]G^G[AT]CC[CT] SfaNI 289 GATGC GCATC(5/9) SfoI 211 GGCGCC GGC^GCC TaqI 625 TCGA T^CGA TseI 18 GCAGC G^C[AT]GC Tsp45I 554 GTGAC ^GT[GC]AC TspRI 412 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 625 TCGA T^CGA Van91I 449 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 31 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcyI 211 GGCGCC G[GA]^CG[CT]C 407 GACGCC AvaII 77 GGTCC G^G[AT]CC 191 GGTCC BpmI 262 CTGGAG CTGGAG(16/14) 294 CTGGAG BsaBI 96 GATAACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 375 GATGACCATC BsaHI 211 GGCGCC G[GA]^CG[CT]C 407 GACGCC BseRI 269 GAGGAG GAGGAG(10/8) 566 GAGGAG BsmFI 78 GTCCC GGGAC(10/14) 192 GTCCC BstF5I 134 GGATG GGATG(2/0) 288 GGATG BstXI 44 CCACCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 513 CCAATATCCTGG BstYI 419 AGATCT [GA]^GATC[CT] 617 AGATCC CfoI 171 GCGC GCG^C 212 GCGC DraII 76 GGGTCCC [GA]G^G[ACGT]CC[CT] 496 GGGGCCT DsaI 88 CCATGG C^C[GA][CT]GG 449 CCATGG EcoO109I 76 GGGTCCC [GA]G^G[ACGT]CC[CT] 496 GGGGCCT EcoT14I 88 CCATGG C^C[AT][AT]GG 449 CCATGG FokI 134 GGATG GGATG(9/13) 288 GGATG HaeIII 86 GGCC GG^CC 498 GGCC HapII 73 CCGG C^CGG 189 CCGG HgaI 407 GACGC GACGC(5/10) 324 GCGTC HhaI 171 GCGC GCG^C 212 GCGC Hin1I 211 GGCGCC G[GA]^CG[CT]C 407 GACGCC HinP1I 171 GCGC G^CGC 212 GCGC HpaII 73 CCGG C^CGG 189 CCGG MamI 96 GATAACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 375 GATGACCATC MboII 93 GAAGA GAAGA(8/7) 483 GAAGA MflI 419 AGATCT [GA]^GATC[CT] 617 AGATCC MspI 73 CCGG C^CGG 189 CCGG NcoI 88 CCATGG C^CATGG 449 CCATGG StyI 88 CCATGG C^C[AT][AT]GG 449 CCATGG TfiI 627 GATTC G^A[AT]TC 635 GAATC XhoII 419 AGATCT [GA]^GATC[CT] 617 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AluI AlwNI BsaJI BsiYI BslI BsmAI BssKI BstNI Cfr13I DdeI DpnII EcoRII HinfI MboI MnlI MvaI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI