(Linear) (Six-base) MAPSORT of: KIEE6637.seq Check: 903 from: 1 to: 645 KIEE6637.orf With 182 enzymes: SgfI * March 16, 2015 11:34 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 217 645 Size: 217 428 AlwNI CAG_nnn'CTG Cuts at: 0 27 152 242 645 Size: 27 125 90 403 Fragments arranged by size: 403 125 90 27 BanI G'GyrC_C Cuts at: 0 211 645 Size: 211 434 BanII G_rGCy'C Cuts at: 0 598 645 Size: 598 47 BbeI G_GCGC'C Cuts at: 0 215 645 Size: 215 430 BclI T'GATC_A Cuts at: 0 108 645 Size: 108 537 BglII A'GATC_T Cuts at: 0 419 645 Size: 419 226 BlpI GC'TnA_GC Cuts at: 0 14 645 Size: 14 631 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 612 645 Size: 612 33 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 283 315 645 Size: 283 32 330 Fragments arranged by size: 330 283 32 BsaBI GATnn'nnATC Cuts at: 0 100 379 645 Size: 100 279 266 Fragments arranged by size: 279 266 100 BsaHI Gr'CG_yC Cuts at: 0 212 408 645 Size: 212 196 237 Fragments arranged by size: 237 212 196 BsaWI w'CCGG_w Cuts at: 0 188 645 Size: 188 457 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 288 318 421 451 645 Size: 288 30 103 30 194 Fragments arranged by size: 288 194 103 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 284 581 645 Size: 284 297 64 Fragments arranged by size: 297 284 64 BseYI C'CCAG_C Cuts at: 0 284 645 Size: 284 361 BsmBI CGTCTCn'nnnn_ Cuts at: 0 331 645 Size: 331 314 Bsp1286I G_dGCh'C Cuts at: 0 598 645 Size: 598 47 BstXI CCAn_nnnn'nTGG Cuts at: 0 51 520 645 Size: 51 469 125 Fragments arranged by size: 469 125 51 BstYI r'GATC_y Cuts at: 0 419 617 645 Size: 419 198 28 Fragments arranged by size: 419 198 28 BtgI C'CryG_G Cuts at: 0 88 449 645 Size: 88 361 196 Fragments arranged by size: 361 196 88 ClaI AT'CG_AT Cuts at: 0 625 645 Size: 625 20 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 328 645 Size: 328 317 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 217 283 315 645 Size: 217 66 32 330 Fragments arranged by size: 330 217 66 32 EcoO109I rG'GnC_Cy Cuts at: 0 77 497 645 Size: 77 420 148 Fragments arranged by size: 420 148 77 EcoRV GAT'ATC Cuts at: 0 526 645 Size: 526 119 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 414 645 Size: 414 231 HaeII r_GCGC'y Cuts at: 0 215 645 Size: 215 430 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 421 453 645 Size: 421 32 192 Fragments arranged by size: 421 192 32 KasI G'GCGC_C Cuts at: 0 211 645 Size: 211 434 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 64 645 Size: 64 581 MslI CAynn'nnrTG Cuts at: 0 49 645 Size: 49 596 NarI GG'CG_CC Cuts at: 0 212 645 Size: 212 433 NcoI C'CATG_G Cuts at: 0 88 449 645 Size: 88 361 196 Fragments arranged by size: 361 196 88 PasI CC'CwG_GG Cuts at: 0 81 645 Size: 81 564 PflMI CCAn_nnn'nTGG Cuts at: 0 455 645 Size: 455 190 PfoI T'CCnGG_A Cuts at: 0 260 519 645 Size: 260 259 126 Fragments arranged by size: 260 259 126 PpuMI rG'GwC_Cy Cuts at: 0 77 645 Size: 77 568 SanDI GG'GwC_CC Cuts at: 0 77 645 Size: 77 568 SfoI GGC'GCC Cuts at: 0 213 645 Size: 213 432 StyI C'CwwG_G Cuts at: 0 88 449 645 Size: 88 361 196 Fragments arranged by size: 361 196 88 XmnI GAAnn'nnTTC Cuts at: 0 35 645 Size: 35 610 Enzymes that do cut: AcuI AlwNI BanI BanII BbeI BclI BglII BlpI BplI BpmI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsmBI Bsp1286I BstXI BstYI BtgI ClaI EciI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I KasI MmeI MslI NarI NcoI PasI PflMI PfoI PpuMI SanDI SfoI StyI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BfrBI BglI Bme1580I BmgBI BmrI BmtI Bpu10I BpuEI BsaI BsaAI BsgI BsiEI BsiHKAI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EcoICRI EcoNI EcoRI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI MspA1I NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI