Restriction map for
pFN21AE6490
(Circular) (Six-base) MAPSORT of: pFN21AE6490.seq Check: 3997 from: 1 to: 6481
pFN21AE6490 6481 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2021 2021
Size: 6481
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4291 278
Size: 53 83 186 936 2755 2468
Fragments arranged by size:
2755 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3824 1937
Size: 1887 4594
Acc65I G'GTAC_C
Cuts at: 3802 3802
Size: 6481
AclI AA'CG_TT
Cuts at: 1563 4608 4981 1563
Size: 3045 373 3063
Fragments arranged by size:
3063 3045 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3227 3676 4544 5928 6196 1632
Size: 1595 449 868 1384 268 1917
Fragments arranged by size:
1917 1595 1384 868 449 268
AflII C'TTAA_G
Cuts at: 820 1017 2252 820
Size: 197 1235 5049
Fragments arranged by size:
5049 1235 197
AflIII A'CryG_T
Cuts at: 1203 5380 6357 1203
Size: 4177 977 1327
Fragments arranged by size:
4177 1327 977
AgeI A'CCGG_T
Cuts at: 5312 5312
Size: 6481
AhdI GACnn_n'nnGTC
Cuts at: 5210 5210
Size: 6481
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6481
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2956 1184
Size: 1772 4709
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2780 2812 2780
Size: 32 6449
AlwNI CAG_nnn'CTG
Cuts at: 2026 3745 4224 5796 2026
Size: 1719 479 1572 2711
Fragments arranged by size:
2711 1719 1572 479
ApaI G_GGCC'C
Cuts at: 2975 2975
Size: 6481
ApaLI G'TGCA_C
Cuts at: 2152 3421 4538 5694 2152
Size: 1269 1117 1156 2939
Fragments arranged by size:
2939 1269 1156 1117
ApoI r'AATT_y
Cuts at: 1493 3790 4016 4168 6277 1493
Size: 2297 226 152 2109 1697
Fragments arranged by size:
2297 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5035 160
Size: 872 4003 1606
Fragments arranged by size:
4003 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6481
AvaI C'yCGr_G
Cuts at: 1870 1943 3039 3615 3806 1870
Size: 73 1096 576 191 4545
Fragments arranged by size:
4545 1096 576 191 73
BamHI G'GATC_C
Cuts at: 3811 4178 3811
Size: 367 6114
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2449 2720 3802 5257 618
Size: 325 217 627 662 271 1082 1455 1842
Fragments arranged by size:
1842 1455 1082 662 627 325 271 217
BanII G_rGCy'C
Cuts at: 1391 2541 2975 3800 1391
Size: 1150 434 825 4072
Fragments arranged by size:
4072 1150 825 434
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3242 3571 928
Size: 971 1343 329 3838
Fragments arranged by size:
3838 1343 971 329
BbvCI CC'TCA_GC
Cuts at: 2437 2437
Size: 6481
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2488 2522 2992 3026 3436 3470 4672
Size: 34 551 34 470 34 410 34 1202
Cuts at: 4672 4706 1903
Size: 34 3678
Fragments arranged by size:
3678 1202 551 470 410 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4373 5583 4373
Size: 1210 5271
BclI T'GATC_A
Cuts at: 1548 3651 1548
Size: 2103 4378
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2030 3473 5092
Size: 107 122 71 631 605 358 1443 1619
Cuts at: 5092 136
Size: 1525
Fragments arranged by size:
1619 1525 1443 631 605 358 122 107
71
BglII A'GATC_T
Cuts at: 6476 6476
Size: 6481
BlpI GC'TnA_GC
Cuts at: 3898 3898
Size: 6481
Bme1580I G_kGCm'C
Cuts at: 1790 2067 2156 2454 2629 2975 3425 3721 4542
Size: 277 89 298 175 346 450 296 821
Cuts at: 4542 5698 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 821 450 346 298 296 277
175 89
BmgBI CAC'GTC
Cuts at: 1318 2604 1318
Size: 1286 5195
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2094 2653 3733
Size: 416 53 83 399 24 677 559 1080
Cuts at: 3733 4212 5170 442
Size: 479 958 1753
Fragments arranged by size:
1753 1080 958 677 559 479 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6481
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2525 3357 5141 1239
Size: 1286 832 1784 2579
Fragments arranged by size:
2579 1784 1286 832
Bpu10I CC'TnA_GC
Cuts at: 2437 2437
Size: 6481
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4606 5471 5769 4606
Size: 865 298 5318
Fragments arranged by size:
5318 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5144 882
Size: 617 3645 2219
Fragments arranged by size:
3645 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4272 493
Size: 711 3068 2702
Fragments arranged by size:
3068 2702 711
BsaBI GATnn'nnATC
Cuts at: 2216 6475 2216
Size: 4259 2222
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4288 4670 5305 275
Size: 53 83 186 936 2755 382 635 1451
Fragments arranged by size:
2755 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2886 4914 5312 5586 5733 6144 2886
Size: 2028 398 274 147 411 3223
Fragments arranged by size:
3223 2028 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2780 2810 1178
Size: 30 165 30 1377 30 4849
Fragments arranged by size:
4849 1377 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2336 2525 3215 3620 1186
Size: 1150 189 690 405 4047
Fragments arranged by size:
4047 1150 690 405 189
BseYI C'CCAG_C
Cuts at: 2968 3063 3277 3334 3675 5684 6180 2968
Size: 95 214 57 341 2009 496 3269
Fragments arranged by size:
3269 2009 496 341 214 95 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2465 3502 3683 1163
Size: 573 729 1037 181 3961
Fragments arranged by size:
3961 1037 729 573 181
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3317 3931 4692 5720 1540
Size: 275 186 1316 614 761 1028 2301
Fragments arranged by size:
2301 1316 1028 761 614 275 186
BsiHKAI G_wGCw'C
Cuts at: 2156 2541 2961 3425 4542 4627 5698 2156
Size: 385 420 464 1117 85 1071 2939
Fragments arranged by size:
2939 1117 1071 464 420 385 85
BsmI GAATG_Cn'
Cuts at: 3992 4085 3992
Size: 93 6388
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2156 2454 2541 2629 2961 2975
Size: 399 277 89 298 87 88 332 14
Cuts at: 2975 3425 3721 3800 4542 4627 5698 1391
Size: 450 296 79 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 450 399 332 298 296
277 89 88 87 85 79 14
BspEI T'CCGG_A
Cuts at: 2886 6144 2886
Size: 3258 3223
BspHI T'CATG_A
Cuts at: 4370 6100 4370
Size: 1730 4751
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2021 2325 3836 844
Size: 1177 304 1511 3489
Fragments arranged by size:
3489 1511 1177 304
BsrBI CCG'CTC
Cuts at: 1394 1823 4368 1394
Size: 429 2545 3507
Fragments arranged by size:
3507 2545 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3378 4976 5150 65
Size: 1183 2130 1598 174 1396
Fragments arranged by size:
2130 1598 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5125 5312 1700
Size: 110 3315 187 2869
Fragments arranged by size:
3315 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6481
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6481
BssSI C'ACGA_G
Cuts at: 4541 5553 4541
Size: 1012 5469
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2965 3364 794
Size: 2171 399 3911
Fragments arranged by size:
3911 2171 399
BstBI TT'CG_AA
Cuts at: 5285 5285
Size: 6481
BstEII G'GTnAC_C
Cuts at: 1184 2566 1184
Size: 1382 5099
BstXI CCAn_nnnn'nTGG
Cuts at: 2302 3535 2302
Size: 1233 5248
BstYI r'GATC_y
Cuts at: 1972 2862 3811 4178 4563 4580 6032 6118 6130
Size: 890 949 367 385 17 1452 86 12
Cuts at: 6130 6476 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 949 890 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2823 3257 3471 513
Size: 552 939 819 434 214 3523
Fragments arranged by size:
3523 939 819 552 434 214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2281 2314 2440 2689 3640 487
Size: 683 56 1055 33 126 249 951 3328
Fragments arranged by size:
3328 1055 951 683 249 126 56 33
BtsI GCAGTG_nn'
Cuts at: 2178 3313 3353 3997 4791 4811 2178
Size: 1135 40 644 794 20 3848
Fragments arranged by size:
3848 1135 794 644 40 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6481
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6446
DraI TTT'AAA
Cuts at: 3785 3785
Size: 6481
DraIII CAC_nnn'GTG
Cuts at: 2661 3420 2661
Size: 759 5722
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5488 809
Size: 507 4172 1802
Fragments arranged by size:
4172 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2172 2292
Size: 54 1425 50 275 18 177 165 120
Cuts at: 2292 2430 3123 3297 3405 3435 3525 3555 3663
Size: 138 693 174 108 30 90 30 108
Cuts at: 3663 3756 3928 4817 6446 8
Size: 93 172 889 1629 43
Fragments arranged by size:
1629 1425 889 693 275 177 174 172
165 138 120 108 108 93 90 54
50 43 30 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 3928 1537
Size: 275 2116 4090
Fragments arranged by size:
4090 2116 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2150 2192 2617 2768 2873 3695 4411 1353
Size: 797 42 425 151 105 822 716 3423
Fragments arranged by size:
3423 822 797 716 425 151 105 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2677 5068 5442 5588 387
Size: 1064 138 1088 2391 374 146 1280
Fragments arranged by size:
2391 1280 1088 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2539 2539
Size: 6481
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2525 3227 3357 3676 4544 5141 5928
Size: 393 893 702 130 319 868 597 787
Cuts at: 5928 6196 1239
Size: 268 1524
Fragments arranged by size:
1524 893 868 787 702 597 393 319
268 130
EcoNI CCTnn'n_nnAGG
Cuts at: 3299 3299
Size: 6481
EcoO109I rG'GnC_Cy
Cuts at: 2023 2161 2590 2972 3184 3211 3592 2023
Size: 138 429 382 212 27 381 4912
Fragments arranged by size:
4912 429 382 381 212 138 27
EcoRI G'AATT_C
Cuts at: 3790 4168 3790
Size: 378 6103
FspI TGC'GCA
Cuts at: 4987 4987
Size: 6481
HaeII r_GCGC'y
Cuts at: 1678 2144 5628 1678
Size: 466 3484 2531
Fragments arranged by size:
3484 2531 466
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2614 2646 3615 3636 3647
Size: 32 135 32 1042 32 969 21 11
Cuts at: 3647 3668 5122 5154 5196 5228 1373
Size: 21 1454 32 42 32 2626
Fragments arranged by size:
2626 1454 1042 969 135 42 32 32
32 32 32 21 21 11
HincII GTy'rAC
Cuts at: 669 1657 1938 3825 4071 669
Size: 988 281 1887 246 3079
Fragments arranged by size:
3079 1887 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2573 748
Size: 1825 4656
HpaI GTT'AAC
Cuts at: 4071 4071
Size: 6481
KpnI G_GTAC'C
Cuts at: 3806 3806
Size: 6481
MfeI C'AATT_G
Cuts at: 4080 4080
Size: 6481
MluI A'CGCG_T
Cuts at: 6357 6357
Size: 6481
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2286 3585 3750 5595 5779 1305
Size: 981 1299 165 1845 184 2007
Fragments arranged by size:
2007 1845 1299 981 184 165
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2294 3125 3299 3407
Size: 54 1425 343 177 285 831 174 108
Cuts at: 3407 3437 3527 3557 3665 3758 10
Size: 30 90 30 108 93 2733
Fragments arranged by size:
2733 1425 831 343 285 177 174 108
108 93 90 54 30 30
MslI CAynn'nnrTG
Cuts at: 518 1166 2135 2300 2447 3020 3143 4439 4798
Size: 648 969 165 147 573 123 1296 359
Cuts at: 4798 4957 518
Size: 159 2042
Fragments arranged by size:
2042 1296 969 648 573 359 165 159
147 123
MspA1I CmG'CkG
Cuts at: 1606 2479 3675 3897 4574 5722 5967 1606
Size: 873 1196 222 677 1148 245 2120
Fragments arranged by size:
2120 1196 1148 873 677 245 222
NcoI C'CATG_G
Cuts at: 513 1065 2004 2823 3471 513
Size: 552 939 819 648 3523
Fragments arranged by size:
3523 939 819 648 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6481
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6481
NotI GC'GGCC_GC
Cuts at: 3928 3928
Size: 6481
NspI r_CATG'y
Cuts at: 1132 1229 2465 3839 5384 1132
Size: 97 1236 1374 1545 2229
Fragments arranged by size:
2229 1545 1374 1236 97
PasI CC'CwG_GG
Cuts at: 1348 3170 3197 1348
Size: 1822 27 4632
Fragments arranged by size:
4632 1822 27
PciI A'CATG_T
Cuts at: 5380 5380
Size: 6481
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2016 1108
Size: 908 5573
PfoI T'CCnGG_A
Cuts at: 2469 2541 2469
Size: 72 6409
PmeI GTTT'AAAC
Cuts at: 3785 3785
Size: 6481
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2780 2812 4550 4582 6063 6095 2780
Size: 32 1738 32 1481 32 3166
Fragments arranged by size:
3166 1738 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3184 3592 3184
Size: 408 6073
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6481
PsiI TTA'TAA
Cuts at: 4051 4051
Size: 6481
PspOMI G'GGCC_C
Cuts at: 2971 2971
Size: 6481
PstI C_TGCA'G
Cuts at: 830 2862 3360 3833 830
Size: 2032 498 473 3478
Fragments arranged by size:
3478 2032 498 473
PvuI CG_AT'CG
Cuts at: 2001 3317 2001
Size: 1316 5165
PvuII CAG'CTG
Cuts at: 2479 3675 2479
Size: 1196 5285
SacI G_AGCT'C
Cuts at: 2541 2541
Size: 6481
SalI G'TCGA_C
Cuts at: 1936 3823 1936
Size: 1887 4594
SapI GCTCTTCn'nnn_
Cuts at: 2150 2768 2150
Size: 618 5863
SbfI CC_TGCA'GG
Cuts at: 3833 3833
Size: 6481
ScaI AGT'ACT
Cuts at: 4729 6441 4729
Size: 1712 4769
SexAI A'CCwGG_T
Cuts at: 2733 2733
Size: 6481
SfcI C'TryA_G
Cuts at: 826 1045 2858 3356 3829 4964 5645 5836 826
Size: 219 1813 498 473 1135 681 191 1471
Fragments arranged by size:
1813 1471 1135 681 498 473 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2030 2030
Size: 6481
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6481
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6481
SmaI CCC'GGG
Cuts at: 1872 3041 3617 3808 1872
Size: 1169 576 191 4545
Fragments arranged by size:
4545 1169 576 191
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2252 4585 5486 5748 820
Size: 197 926 309 2333 901 262 1553
Fragments arranged by size:
2333 1553 926 901 309 262 197
SnaBI TAC'GTA
Cuts at: 493 4272 493
Size: 3779 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6481
SphI G_CATG'C
Cuts at: 1132 3839 1132
Size: 2707 3774
SrfI GCCC'GGGC
Cuts at: 3617 3617
Size: 6481
SspI AAT'ATT
Cuts at: 5 52 4405 5
Size: 47 4353 2081
Fragments arranged by size:
4353 2081 47
StuI AGG'CCT
Cuts at: 1517 2111 1517
Size: 594 5887
StyI C'CwwG_G
Cuts at: 513 1065 2004 2823 3090 3300 3471 3920 513
Size: 552 939 819 267 210 171 449 3074
Fragments arranged by size:
3074 939 819 552 449 267 210 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2672 2743 2969 3269 4508 4525 4678
Size: 1255 1092 71 226 300 1239 17 153
Cuts at: 4678 4863 5308 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1239 1092 445 300 226 185
153 71 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4727 6439 96
Size: 274 80 33 51 1443 2750 1712 138
Fragments arranged by size:
2750 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2451 2483 6441 6473 2451
Size: 32 3958 32 2459
Fragments arranged by size:
3958 2459 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6481
XbaI T'CTAG_A
Cuts at: 3817 3817
Size: 6481
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2134 2134
Size: 6481
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6481
XmaI C'CCGG_G
Cuts at: 1870 3039 3615 3806 1870
Size: 1169 576 191 4545
Fragments arranged by size:
4545 1169 576 191
XmnI GAAnn'nnTTC
Cuts at: 4610 4610
Size: 6481
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4289 276
Size: 53 83 186 936 2755 2468
Fragments arranged by size:
2755 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SanDI SfoI SwaI