(Linear) (Six-base) MAPSORT of: KIEE6490.seq Check: 585 from: 1 to: 1776 KIEE6490.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 16 1776 Size: 16 1760 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1222 1671 1776 Size: 1222 449 105 Fragments arranged by size: 1222 449 105 AflII C'TTAA_G Cuts at: 0 247 1776 Size: 247 1529 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 951 1776 Size: 951 825 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 775 807 1776 Size: 775 32 969 Fragments arranged by size: 969 775 32 AlwNI CAG_nnn'CTG Cuts at: 0 21 1740 1776 Size: 21 1719 36 Fragments arranged by size: 1719 36 21 ApaI G_GGCC'C Cuts at: 0 970 1776 Size: 970 806 ApaLI G'TGCA_C Cuts at: 0 147 1416 1776 Size: 147 1269 360 Fragments arranged by size: 1269 360 147 AvaI C'yCGr_G Cuts at: 0 1034 1610 1776 Size: 1034 576 166 Fragments arranged by size: 1034 576 166 BanI G'GyrC_C Cuts at: 0 444 715 1776 Size: 444 271 1061 Fragments arranged by size: 1061 444 271 BanII G_rGCy'C Cuts at: 0 536 970 1776 Size: 536 434 806 Fragments arranged by size: 806 536 434 BbsI GAAGACnn'nnnn_ Cuts at: 0 1237 1566 1776 Size: 1237 329 210 Fragments arranged by size: 1237 329 210 BbvCI CC'TCA_GC Cuts at: 0 432 1776 Size: 432 1344 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 483 517 987 1021 1431 1465 1776 Size: 483 34 470 34 410 34 311 Fragments arranged by size: 483 470 410 311 34 34 34 BclI T'GATC_A Cuts at: 0 1646 1776 Size: 1646 130 BglI GCCn_nnn'nGGC Cuts at: 0 25 1468 1776 Size: 25 1443 308 Fragments arranged by size: 1443 308 25 Bme1580I G_kGCm'C Cuts at: 0 62 151 449 624 970 1420 1716 1776 Size: 62 89 298 175 346 450 296 60 Fragments arranged by size: 450 346 298 296 175 89 62 60 BmgBI CAC'GTC Cuts at: 0 599 1776 Size: 599 1177 BmrI ACTGGGnnnn_n' Cuts at: 0 89 648 1728 1776 Size: 89 559 1080 48 Fragments arranged by size: 1080 559 89 48 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 520 1352 1776 Size: 520 832 424 Fragments arranged by size: 832 520 424 Bpu10I CC'TnA_GC Cuts at: 0 432 1776 Size: 432 1344 BsaBI GATnn'nnATC Cuts at: 0 211 1776 Size: 211 1565 BsaWI w'CCGG_w Cuts at: 0 881 1776 Size: 881 895 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 775 805 1776 Size: 775 30 971 Fragments arranged by size: 971 775 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 331 520 1210 1615 1776 Size: 331 189 690 405 161 Fragments arranged by size: 690 405 331 189 161 BseYI C'CCAG_C Cuts at: 0 963 1058 1272 1329 1670 1776 Size: 963 95 214 57 341 106 Fragments arranged by size: 963 341 214 106 95 57 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 460 1497 1678 1776 Size: 460 1037 181 98 Fragments arranged by size: 1037 460 181 98 BsiEI CG_ry'CG Cuts at: 0 1312 1776 Size: 1312 464 BsiHKAI G_wGCw'C Cuts at: 0 151 536 956 1420 1776 Size: 151 385 420 464 356 Fragments arranged by size: 464 420 385 356 151 Bsp1286I G_dGCh'C Cuts at: 0 62 151 449 536 624 956 970 1420 Size: 62 89 298 87 88 332 14 450 Cuts at: 1420 1716 1776 Size: 296 60 Fragments arranged by size: 450 332 298 296 89 88 87 62 60 14 BspEI T'CCGG_A Cuts at: 0 881 1776 Size: 881 895 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 16 320 1776 Size: 16 304 1456 Fragments arranged by size: 1456 304 16 BsrDI GCAATG_nn' Cuts at: 0 1373 1776 Size: 1373 403 BstAPI GCAn_nnn'nTGC Cuts at: 0 960 1359 1776 Size: 960 399 417 Fragments arranged by size: 960 417 399 BstEII G'GTnAC_C Cuts at: 0 561 1776 Size: 561 1215 BstXI CCAn_nnnn'nTGG Cuts at: 0 297 1530 1776 Size: 297 1233 246 Fragments arranged by size: 1233 297 246 BstYI r'GATC_y Cuts at: 0 857 1776 Size: 857 919 BtgI C'CryG_G Cuts at: 0 818 1252 1466 1776 Size: 818 434 214 310 Fragments arranged by size: 818 434 310 214 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 276 309 435 684 1635 1776 Size: 276 33 126 249 951 141 Fragments arranged by size: 951 276 249 141 126 33 BtsI GCAGTG_nn' Cuts at: 0 173 1308 1348 1776 Size: 173 1135 40 428 Fragments arranged by size: 1135 428 173 40 DraIII CAC_nnn'GTG Cuts at: 0 656 1415 1776 Size: 656 759 361 Fragments arranged by size: 759 656 361 EaeI y'GGCC_r Cuts at: 0 2 167 287 425 1118 1292 1400 1430 Size: 2 165 120 138 693 174 108 30 Cuts at: 1430 1520 1550 1658 1751 1776 Size: 90 30 108 93 25 Fragments arranged by size: 693 174 165 138 120 108 108 93 90 30 30 25 2 EarI CTCTTCn'nnn_ Cuts at: 0 145 187 612 763 868 1690 1776 Size: 145 42 425 151 105 822 86 Fragments arranged by size: 822 425 151 145 105 86 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 672 1776 Size: 672 1104 EcoICRI GAG'CTC Cuts at: 0 534 1776 Size: 534 1242 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 520 1222 1352 1671 1776 Size: 520 702 130 319 105 Fragments arranged by size: 702 520 319 130 105 EcoNI CCTnn'n_nnAGG Cuts at: 0 1294 1776 Size: 1294 482 EcoO109I rG'GnC_Cy Cuts at: 0 18 156 585 967 1179 1206 1587 1776 Size: 18 138 429 382 212 27 381 189 Fragments arranged by size: 429 382 381 212 189 138 27 18 HaeII r_GCGC'y Cuts at: 0 139 1776 Size: 139 1637 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 609 641 1610 1631 1642 1663 1776 Size: 609 32 969 21 11 21 113 Fragments arranged by size: 969 609 113 32 21 21 11 HindIII A'AGCT_T Cuts at: 0 568 1776 Size: 568 1208 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 281 1580 1745 1776 Size: 281 1299 165 31 Fragments arranged by size: 1299 281 165 31 MscI TGG'CCA Cuts at: 0 4 289 1120 1294 1402 1432 1522 1552 Size: 4 285 831 174 108 30 90 30 Cuts at: 1552 1660 1753 1776 Size: 108 93 23 Fragments arranged by size: 831 285 174 108 108 93 90 30 30 23 4 MslI CAynn'nnrTG Cuts at: 0 130 295 442 1015 1138 1776 Size: 130 165 147 573 123 638 Fragments arranged by size: 638 573 165 147 130 123 MspA1I CmG'CkG Cuts at: 0 474 1670 1776 Size: 474 1196 106 Fragments arranged by size: 1196 474 106 NcoI C'CATG_G Cuts at: 0 818 1466 1776 Size: 818 648 310 Fragments arranged by size: 818 648 310 NspI r_CATG'y Cuts at: 0 460 1776 Size: 460 1316 PasI CC'CwG_GG Cuts at: 0 1165 1192 1776 Size: 1165 27 584 Fragments arranged by size: 1165 584 27 PflMI CCAn_nnn'nTGG Cuts at: 0 11 1776 Size: 11 1765 PfoI T'CCnGG_A Cuts at: 0 464 536 1776 Size: 464 72 1240 Fragments arranged by size: 1240 464 72 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 775 807 1776 Size: 775 32 969 Fragments arranged by size: 969 775 32 PpuMI rG'GwC_Cy Cuts at: 0 1179 1587 1776 Size: 1179 408 189 Fragments arranged by size: 1179 408 189 PspOMI G'GGCC_C Cuts at: 0 966 1776 Size: 966 810 PstI C_TGCA'G Cuts at: 0 857 1355 1776 Size: 857 498 421 Fragments arranged by size: 857 498 421 PvuI CG_AT'CG Cuts at: 0 1312 1776 Size: 1312 464 PvuII CAG'CTG Cuts at: 0 474 1670 1776 Size: 474 1196 106 Fragments arranged by size: 1196 474 106 SacI G_AGCT'C Cuts at: 0 536 1776 Size: 536 1240 SapI GCTCTTCn'nnn_ Cuts at: 0 145 763 1776 Size: 145 618 1013 Fragments arranged by size: 1013 618 145 SexAI A'CCwGG_T Cuts at: 0 728 1776 Size: 728 1048 SfcI C'TryA_G Cuts at: 0 853 1351 1776 Size: 853 498 425 Fragments arranged by size: 853 498 425 SfiI GGCCn_nnn'nGGCC Cuts at: 0 25 1776 Size: 25 1751 SmaI CCC'GGG Cuts at: 0 1036 1612 1776 Size: 1036 576 164 Fragments arranged by size: 1036 576 164 SmlI C'TyrA_G Cuts at: 0 247 1776 Size: 247 1529 SrfI GCCC'GGGC Cuts at: 0 1612 1776 Size: 1612 164 StuI AGG'CCT Cuts at: 0 106 1776 Size: 106 1670 StyI C'CwwG_G Cuts at: 0 818 1085 1295 1466 1776 Size: 818 267 210 171 310 Fragments arranged by size: 818 310 267 210 171 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 667 738 964 1264 1776 Size: 667 71 226 300 512 Fragments arranged by size: 667 512 300 226 71 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 446 478 1776 Size: 446 32 1298 Fragments arranged by size: 1298 446 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 129 1776 Size: 129 1647 XmaI C'CCGG_G Cuts at: 0 1034 1610 1776 Size: 1034 576 166 Fragments arranged by size: 1034 576 166 Enzymes that do cut: AarI AcuI AflII AlfI AloI AlwNI ApaI ApaLI AvaI BanI BanII BbsI BbvCI BcgI BclI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrDI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII MmeI MscI MslI MspA1I NcoI NspI PasI PflMI PfoI PpiI PpuMI PspOMI PstI PvuI PvuII SacI SapI SexAI SfcI SfiI SmaI SmlI SrfI StuI StyI TaqII TstI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI AleI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BfrBI BglII BlpI BmtI BplI BpuEI BsaI BsaAI BsaHI BsiWI BsmI BsmBI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PshAI PsiI PspXI PsrI RsrII SacII SalI SanDI SbfI ScaI SfoI SgfI SgrAI SnaBI SpeI SphI SspI SwaI TatI Tth111I XbaI XhoI XmnI ZraI