################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AviII AvrII BbeI BbvCI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaHI BsaI BsaWI BseAI BsmBI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PleI PmeI Psp1406I PvuI RcaI RsrII SacI SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI StuI StyI SwaI Tth111I Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AlwNI 319 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 1114 ATTAAT AT^TAAT AsnI 1114 ATTAAT AT^TAAT AspHI 1030 GTGCTC G[AT]GC[AT]^C BamHI 1470 GGATCC G^GATCC BanI 1487 GGTGCC G^G[CT][GA]CC BanII 16 GAGCCC G[GA]GC[CT]^C BbsI 1657 GTCTTC GAAGAC(2/6) BclI 161 TGATCA T^GATCA BfaI 1360 CTAG C^TAG BglI 191 GCCACCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 1657 GTCTTC GAAGAC(2/6) BsaBI 1323 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 647 CTGCAC GTGCAG(16/14) BsiEI 1101 CGACCG CG[GA][CT]^CG BsiHKAI 1030 GTGCTC G[AT]GC[AT]^C BsiWI 308 CGTACG C^GTACG BssSI 1179 CACGAG CACGAG(-5/-1) BstAPI 414 GCACATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 1470 GGATCC [GA]^GATC[CT] DraII 437 AGGACCC [GA]G^G[ACGT]CC[CT] DsaI 60 CCGTGG C^C[GA][CT]GG EcoO109I 437 AGGACCC [GA]G^G[ACGT]CC[CT] FbaI 161 TGATCA T^GATCA MaeI 1360 CTAG C^TAG MamI 1323 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1470 GGATCC [GA]^GATC[CT] NspI 797 GCATGC [GA]CATG^[CT] PaeR7I 748 CTCGAG C^TCGAG PpuMI 437 AGGACCC [GA]G^G[AT]CC[CT] PshAI 465 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1114 ATTAAT AT^TAAT SphI 797 GCATGC GCATG^C SspI 527 AATATT AAT^ATT TaqI 749 TCGA T^CGA TthHB8I 749 TCGA T^CGA XhoI 748 CTCGAG C^TCGAG XhoII 1470 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AocI 10 CCTGAGG CC^T[ACGT]AGG 1465 CCTCAGG AvaI 706 CTCGGG C^[CT]CG[GA]G 748 CTCGAG BbrPI 331 CACGTG CAC^GTG 1078 CACGTG BcnI 39 CCCGG CC^[GC]GG 954 CCGGG BsaAI 331 CACGTG [CT]AC^GT[GA] 1078 CACGTG BsmFI 744 GTCCC GGGAC(10/14) 922 GTCCC BsoBI 706 CTCGGG C^[CT]CG[GA]G 748 CTCGAG Bsp1407I 914 TGTACA T^GTACA 1333 TGTACA BsrGI 914 TGTACA T^GTACA 1333 TGTACA Bsu36I 10 CCTGAGG CC^T[ACGT]AGG 1465 CCTCAGG Eco81I 10 CCTGAGG CC^T[ACGT]AGG 1465 CCTCAGG EcoRI 1452 GAATTC G^AATTC 1703 GAATTC HinfI 266 GATTC G^A[ACGT]TC 1691 GATTC HphI 636 GGTGA GGTGA(8/7) 1479 TCACC MslI 798 CATGCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 906 CATCATTGTG NciI 39 CCCGG CC^[GC]GG 954 CCGGG PmaCI 331 CACGTG CAC^GTG 1078 CACGTG PmlI 331 CACGTG CAC^GTG 1078 CACGTG PvuII 145 CAGCTG CAG^CTG 1621 CAGCTG SbfI 756 CCTGCAGG CCTGCA^GG 925 CCTGCAGG SmlI 52 CTTGAG C^T[CT][GA]AG 748 CTCGAG Sse8387I 756 CCTGCAGG CCTGCA^GG 925 CCTGCAGG SspBI 914 TGTACA T^GTACA 1333 TGTACA TfiI 266 GATTC G^A[AT]TC 1691 GATTC XcmI 558 CCAGATCGTAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1613 CCAGGAAACAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AvaII BalI BbvI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI Tru9I TseI Tsp45I Tsp509I TspRI