(Circular) (Six-base) MAPSORT of: pFN21AE2411.seq Check: 5266 from: 1 to: 6415 pFN21AE2411 6415 bp With 182 enzymes: SgfI * May 11, 2012 14:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4225 278 Size: 53 83 186 936 2689 2468 Fragments arranged by size: 2689 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3758 1937 Size: 1821 4594 Acc65I G'GTAC_C Cuts at: 3736 3736 Size: 6415 AclI AA'CG_TT Cuts at: 1563 4542 4915 1563 Size: 2979 373 3063 Fragments arranged by size: 3063 2979 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2351 2419 2923 3037 4478 5862 6130 1632 Size: 719 68 504 114 1441 1384 268 1917 Fragments arranged by size: 1917 1441 1384 719 504 268 114 68 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6218 AflIII A'CryG_T Cuts at: 1203 5314 6291 1203 Size: 4111 977 1327 Fragments arranged by size: 4111 1327 977 AgeI A'CCGG_T Cuts at: 5246 5246 Size: 6415 AhdI GACnn_n'nnGTC Cuts at: 5144 5144 Size: 6415 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6415 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2376 2825 1184 Size: 1192 449 4774 Fragments arranged by size: 4774 1192 449 AlwNI CAG_nnn'CTG Cuts at: 2329 4158 5730 2329 Size: 1829 1572 3014 Fragments arranged by size: 3014 1829 1572 ApaLI G'TGCA_C Cuts at: 4472 5628 4472 Size: 1156 5259 ApoI r'AATT_y Cuts at: 1493 3457 3513 3708 3724 3950 4102 6211 1493 Size: 1964 56 195 16 226 152 2109 1697 Fragments arranged by size: 2109 1964 1697 226 195 152 56 16 AseI AT'TA_AT Cuts at: 160 1032 3120 4969 160 Size: 872 2088 1849 1606 Fragments arranged by size: 2088 1849 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6415 AvaI C'yCGr_G Cuts at: 1870 1943 2711 2753 3740 1870 Size: 73 768 42 987 4545 Fragments arranged by size: 4545 987 768 73 42 BamHI G'GATC_C Cuts at: 3475 3745 4112 3475 Size: 270 367 5778 Fragments arranged by size: 5778 367 270 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3492 3736 5191 618 Size: 325 217 627 1705 244 1455 1842 Fragments arranged by size: 1842 1705 1455 627 325 244 217 BanII G_rGCy'C Cuts at: 1391 2025 3734 1391 Size: 634 1709 4072 Fragments arranged by size: 4072 1709 634 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3655 928 Size: 971 1756 3688 Fragments arranged by size: 3688 1756 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4606 4640 1903 Size: 34 2669 34 3678 Fragments arranged by size: 3678 2669 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4307 5517 4307 Size: 1210 5205 BclI T'GATC_A Cuts at: 1548 2166 1548 Size: 618 5797 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2202 5026 136 Size: 107 122 71 631 605 530 2824 1525 Fragments arranged by size: 2824 1525 631 605 530 122 107 71 BglII A'GATC_T Cuts at: 6410 6410 Size: 6415 BlpI GC'TnA_GC Cuts at: 3832 3832 Size: 6415 Bme1580I G_kGCm'C Cuts at: 1790 3497 4476 5632 1790 Size: 1707 979 1156 2573 Fragments arranged by size: 2573 1707 1156 979 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6415 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2668 2779 4146 Size: 416 53 83 399 24 1251 111 1367 Cuts at: 4146 5104 442 Size: 958 1753 Fragments arranged by size: 1753 1367 1251 958 416 399 111 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6415 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2231 2595 3003 5075 1239 Size: 992 364 408 2072 2579 Fragments arranged by size: 2579 2072 992 408 364 Bpu10I CC'TnA_GC Cuts at: 3132 3411 3132 Size: 279 6136 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2078 4540 5405 5703 2078 Size: 2462 865 298 2790 Fragments arranged by size: 2790 2462 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5078 882 Size: 617 3579 2219 Fragments arranged by size: 3579 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2338 3085 4206 493 Size: 711 1134 747 1121 2702 Fragments arranged by size: 2702 1134 1121 747 711 BsaBI GATnn'nnATC Cuts at: 3332 6409 3332 Size: 3077 3338 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4222 4604 5239 275 Size: 53 83 186 936 2689 382 635 1451 Fragments arranged by size: 2689 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4848 5246 5520 5667 6078 4848 Size: 398 274 147 411 5185 Fragments arranged by size: 5185 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2759 2789 3219 3249 1178 Size: 30 165 30 1356 30 430 30 4344 Fragments arranged by size: 4344 1356 430 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 2366 2684 1186 Size: 847 333 318 4917 Fragments arranged by size: 4917 847 333 318 BseYI C'CCAG_C Cuts at: 2394 2415 2798 2878 5618 6114 2394 Size: 21 383 80 2740 496 2695 Fragments arranged by size: 2740 2695 496 383 80 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2637 1163 Size: 573 901 4941 Fragments arranged by size: 4941 901 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3109 3865 4626 5654 1540 Size: 275 186 1108 756 761 1028 2301 Fragments arranged by size: 2301 1108 1028 761 756 275 186 BsiHKAI G_wGCw'C Cuts at: 3039 4476 4561 5632 3039 Size: 1437 85 1071 3822 Fragments arranged by size: 3822 1437 1071 85 BsiWI C'GTAC_G Cuts at: 2313 2313 Size: 6415 BsmI GAATG_Cn' Cuts at: 3926 4019 3926 Size: 93 6322 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 3039 3497 3734 4476 4561 5632 Size: 399 235 1014 458 237 742 85 1071 Cuts at: 5632 1391 Size: 2174 Fragments arranged by size: 2174 1071 1014 742 458 399 237 235 85 BspEI T'CCGG_A Cuts at: 6078 6078 Size: 6415 BspHI T'CATG_A Cuts at: 4304 6034 4304 Size: 1730 4685 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3770 844 Size: 2926 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4302 1394 Size: 429 2479 3507 Fragments arranged by size: 3507 2479 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4910 5084 65 Size: 1183 3662 174 1396 Fragments arranged by size: 3662 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5059 5246 1700 Size: 110 3249 187 2869 Fragments arranged by size: 3249 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2919 3338 96 Size: 2823 419 3173 Fragments arranged by size: 3173 2823 419 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6415 BssSI C'ACGA_G Cuts at: 3184 4475 5487 3184 Size: 1291 1012 4112 Fragments arranged by size: 4112 1291 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2425 794 Size: 1631 4784 BstBI TT'CG_AA Cuts at: 5219 5219 Size: 6415 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6415 BstYI r'GATC_y Cuts at: 1972 3475 3745 4112 4497 4514 5966 6052 6064 Size: 1503 270 367 385 17 1452 86 12 Cuts at: 6064 6410 1972 Size: 346 1977 Fragments arranged by size: 1977 1503 1452 385 367 346 270 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2016 3471 2016 Size: 1455 4960 BtgI C'CryG_G Cuts at: 513 1065 2004 2065 513 Size: 552 939 61 4863 Fragments arranged by size: 4863 939 552 61 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5676 Fragments arranged by size: 5676 683 56 BtsI GCAGTG_nn' Cuts at: 3931 4725 4745 3931 Size: 794 20 5601 Fragments arranged by size: 5601 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6415 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6380 DraI TTT'AAA Cuts at: 3719 3719 Size: 6415 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5422 809 Size: 507 4106 1802 Fragments arranged by size: 4106 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2194 2790 2907 Size: 54 1425 50 275 18 364 596 117 Cuts at: 2907 3862 4751 6380 8 Size: 955 889 1629 43 Fragments arranged by size: 1629 1425 955 889 596 364 275 117 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3862 1537 Size: 275 2050 4090 Fragments arranged by size: 4090 2050 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2097 2438 2502 4345 1353 Size: 744 341 64 1843 3423 Fragments arranged by size: 3423 1843 744 341 64 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2080 2774 5002 5376 5522 387 Size: 1064 138 491 694 2228 374 146 1280 Fragments arranged by size: 2228 1280 1064 694 491 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 2351 2419 2595 2923 3003 3037 Size: 393 599 120 68 176 328 80 34 Cuts at: 3037 4478 5075 5862 6130 1239 Size: 1441 597 787 268 1524 Fragments arranged by size: 1524 1441 787 599 597 393 328 268 176 120 80 68 34 EcoO109I rG'GnC_Cy Cuts at: 2443 2443 Size: 6415 EcoRI G'AATT_C Cuts at: 3457 3708 3724 4102 3457 Size: 251 16 378 5770 Fragments arranged by size: 5770 378 251 16 FspI TGC'GCA Cuts at: 4921 4921 Size: 6415 HaeII r_GCGC'y Cuts at: 1678 2117 2402 2886 3609 5562 1678 Size: 439 285 484 723 1953 2531 Fragments arranged by size: 2531 1953 723 484 439 285 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2759 2791 3199 3231 3536 Size: 32 135 32 1187 32 408 32 305 Cuts at: 3536 3568 3687 3719 5056 5088 5130 5162 1373 Size: 32 119 32 1337 32 42 32 2626 Fragments arranged by size: 2626 1337 1187 408 305 135 119 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3759 4005 669 Size: 988 281 1821 246 3079 Fragments arranged by size: 3079 1821 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6415 HpaI GTT'AAC Cuts at: 4005 4005 Size: 6415 KpnI G_GTAC'C Cuts at: 3740 3740 Size: 6415 MfeI C'AATT_G Cuts at: 4014 4014 Size: 6415 MluI A'CGCG_T Cuts at: 6291 6291 Size: 6415 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3205 3511 5529 5713 1305 Size: 1900 306 2018 184 2007 Fragments arranged by size: 2018 2007 1900 306 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2196 2792 2909 10 Size: 54 1425 343 364 596 117 3516 Fragments arranged by size: 3516 1425 596 364 343 117 54 MslI CAynn'nnrTG Cuts at: 518 1166 2807 2915 4373 4732 4891 518 Size: 648 1641 108 1458 359 159 2042 Fragments arranged by size: 2042 1641 1458 648 359 159 108 MspA1I CmG'CkG Cuts at: 1606 2152 2356 3628 3831 4508 5656 5901 1606 Size: 546 204 1272 203 677 1148 245 2120 Fragments arranged by size: 2120 1272 1148 677 546 245 204 203 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4924 Fragments arranged by size: 4924 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6415 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6415 NotI GC'GGCC_GC Cuts at: 3862 3862 Size: 6415 NspI r_CATG'y Cuts at: 1132 1229 2806 3773 5318 1132 Size: 97 1577 967 1545 2229 Fragments arranged by size: 2229 1577 1545 967 97 PasI CC'CwG_GG Cuts at: 1348 3488 1348 Size: 2140 4275 PciI A'CATG_T Cuts at: 5314 5314 Size: 6415 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6415 PfoI T'CCnGG_A Cuts at: 3617 3617 Size: 6415 PmeI GTTT'AAAC Cuts at: 3719 3719 Size: 6415 PmlI CAC'GTG Cuts at: 2338 3085 2338 Size: 747 5668 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4484 4516 5997 6029 4484 Size: 32 1481 32 4870 Fragments arranged by size: 4870 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2443 2443 Size: 6415 PshAI GACnn'nnGTC Cuts at: 1613 2474 1613 Size: 861 5554 PsiI TTA'TAA Cuts at: 3985 3985 Size: 6415 PspXI vC'TCGA_Gb Cuts at: 2753 2753 Size: 6415 PstI C_TGCA'G Cuts at: 830 2355 2766 2935 3767 830 Size: 1525 411 169 832 3478 Fragments arranged by size: 3478 1525 832 411 169 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6415 PvuII CAG'CTG Cuts at: 2152 3628 2152 Size: 1476 4939 SalI G'TCGA_C Cuts at: 1936 3757 1936 Size: 1821 4594 SapI GCTCTTCn'nnn_ Cuts at: 2097 2438 2502 2097 Size: 341 64 6010 Fragments arranged by size: 6010 341 64 SbfI CC_TGCA'GG Cuts at: 2766 2935 3767 2766 Size: 169 832 5414 Fragments arranged by size: 5414 832 169 ScaI AGT'ACT Cuts at: 4663 6375 4663 Size: 1712 4703 SfcI C'TryA_G Cuts at: 826 1045 2351 2762 2931 3045 3763 4898 5579 Size: 219 1306 411 169 114 718 1135 681 Cuts at: 5579 5770 826 Size: 191 1471 Fragments arranged by size: 1471 1306 1135 718 681 411 219 191 169 114 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6415 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6415 SmaI CCC'GGG Cuts at: 1872 3742 1872 Size: 1870 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2057 2753 4519 5420 5682 820 Size: 197 926 114 696 1766 901 262 1553 Fragments arranged by size: 1766 1553 926 901 696 262 197 114 SnaBI TAC'GTA Cuts at: 493 4206 493 Size: 3713 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6415 SphI G_CATG'C Cuts at: 1132 2806 3773 1132 Size: 1674 967 3774 Fragments arranged by size: 3774 1674 967 SspI AAT'ATT Cuts at: 5 52 2534 4339 5 Size: 47 2482 1805 2081 Fragments arranged by size: 2482 2081 1805 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6415 StyI C'CwwG_G Cuts at: 513 1065 2004 3854 513 Size: 552 939 1850 3074 Fragments arranged by size: 3074 1850 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2429 2966 3123 3501 4442 4459 4612 Size: 1255 849 537 157 378 941 17 153 Cuts at: 4612 4797 5242 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 941 849 537 445 378 185 157 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2554 2676 2919 Size: 274 80 33 51 1443 577 122 243 Cuts at: 2919 3338 4661 6373 96 Size: 419 1323 1712 138 Fragments arranged by size: 1712 1443 1323 577 419 274 243 138 122 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3219 3251 6375 6407 3219 Size: 32 3124 32 3227 Fragments arranged by size: 3227 3124 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6415 XbaI T'CTAG_A Cuts at: 3751 3751 Size: 6415 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2570 3625 2570 Size: 1055 5360 XhoI C'TCGA_G Cuts at: 1943 2753 1943 Size: 810 5605 XmaI C'CCGG_G Cuts at: 1870 3740 1870 Size: 1870 4545 XmnI GAAnn'nnTTC Cuts at: 4544 4544 Size: 6415 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4223 276 Size: 53 83 186 936 2689 2468 Fragments arranged by size: 2689 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI