Restriction map for
pFN21AE2411
(Linear) (Six-base) MAPSORT of: KIEE2411.seq Check: 1360 from: 1 to: 1710
KIEE2411.orf
With 182 enzymes: SgfI *
May 11, 2012 13:47 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 414 918 1032 1710
Size: 346 68 504 114 678
Fragments arranged by size:
678 504 346 114 68
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 371 820 1710
Size: 371 449 890
Fragments arranged by size:
890 449 371
AlwNI CAG_nnn'CTG
Cuts at: 0 324 1710
Size: 324 1386
ApoI r'AATT_y
Cuts at: 0 1452 1508 1703 1710
Size: 1452 56 195 7
Fragments arranged by size:
1452 195 56 7
AseI AT'TA_AT
Cuts at: 0 1115 1710
Size: 1115 595
AvaI C'yCGr_G
Cuts at: 0 706 748 1710
Size: 706 42 962
Fragments arranged by size:
962 706 42
BamHI G'GATC_C
Cuts at: 0 1470 1710
Size: 1470 240
BanI G'GyrC_C
Cuts at: 0 1487 1710
Size: 1487 223
BanII G_rGCy'C
Cuts at: 0 20 1710
Size: 20 1690
BbsI GAAGACnn'nnnn_
Cuts at: 0 1650 1710
Size: 1650 60
BclI T'GATC_A
Cuts at: 0 161 1710
Size: 161 1549
BglI GCCn_nnn'nGGC
Cuts at: 0 197 1710
Size: 197 1513
Bme1580I G_kGCm'C
Cuts at: 0 1492 1710
Size: 1492 218
BmrI ACTGGGnnnn_n'
Cuts at: 0 663 774 1710
Size: 663 111 936
Fragments arranged by size:
936 663 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 590 998 1710
Size: 226 364 408 712
Fragments arranged by size:
712 408 364 226
Bpu10I CC'TnA_GC
Cuts at: 0 1127 1406 1710
Size: 1127 279 304
Fragments arranged by size:
1127 304 279
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 1710
Size: 73 1637
BsaAI yAC'GTr
Cuts at: 0 333 1080 1710
Size: 333 747 630
Fragments arranged by size:
747 630 333
BsaBI GATnn'nnATC
Cuts at: 0 1327 1710
Size: 1327 383
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 754 784 1214 1244 1710
Size: 754 30 430 30 466
Fragments arranged by size:
754 466 430 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 361 679 1710
Size: 28 333 318 1031
Fragments arranged by size:
1031 333 318 28
BseYI C'CCAG_C
Cuts at: 0 389 410 793 873 1710
Size: 389 21 383 80 837
Fragments arranged by size:
837 389 383 80 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 632 1710
Size: 632 1078
BsiEI CG_ry'CG
Cuts at: 0 1104 1710
Size: 1104 606
BsiHKAI G_wGCw'C
Cuts at: 0 1034 1710
Size: 1034 676
BsiWI C'GTAC_G
Cuts at: 0 308 1710
Size: 308 1402
Bsp1286I G_dGCh'C
Cuts at: 0 20 1034 1492 1710
Size: 20 1014 458 218
Fragments arranged by size:
1014 458 218 20
BsrGI T'GTAC_A
Cuts at: 0 914 1333 1710
Size: 914 419 377
Fragments arranged by size:
914 419 377
BssSI C'ACGA_G
Cuts at: 0 1179 1710
Size: 1179 531
BstAPI GCAn_nnn'nTGC
Cuts at: 0 420 1710
Size: 420 1290
BstYI r'GATC_y
Cuts at: 0 1470 1710
Size: 1470 240
Bsu36I CC'TnA_GG
Cuts at: 0 11 1466 1710
Size: 11 1455 244
Fragments arranged by size:
1455 244 11
BtgI C'CryG_G
Cuts at: 0 60 1710
Size: 60 1650
EaeI y'GGCC_r
Cuts at: 0 189 785 902 1710
Size: 189 596 117 808
Fragments arranged by size:
808 596 189 117
EarI CTCTTCn'nnn_
Cuts at: 0 92 433 497 1710
Size: 92 341 64 1213
Fragments arranged by size:
1213 341 92 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 75 769 1710
Size: 75 694 941
Fragments arranged by size:
941 694 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 346 414 590 918 998 1032 1710
Size: 226 120 68 176 328 80 34 678
Fragments arranged by size:
678 328 226 176 120 80 68 34
EcoO109I rG'GnC_Cy
Cuts at: 0 438 1710
Size: 438 1272
EcoRI G'AATT_C
Cuts at: 0 1452 1703 1710
Size: 1452 251 7
Fragments arranged by size:
1452 251 7
HaeII r_GCGC'y
Cuts at: 0 112 397 881 1604 1710
Size: 112 285 484 723 106
Fragments arranged by size:
723 484 285 112 106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 754 786 1194 1226 1531 1563 1682 1710
Size: 754 32 408 32 305 32 119 28
Fragments arranged by size:
754 408 305 119 32 32 32 28
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1200 1506 1710
Size: 1200 306 204
Fragments arranged by size:
1200 306 204
MscI TGG'CCA
Cuts at: 0 191 787 904 1710
Size: 191 596 117 806
Fragments arranged by size:
806 596 191 117
MslI CAynn'nnrTG
Cuts at: 0 802 910 1710
Size: 802 108 800
Fragments arranged by size:
802 800 108
MspA1I CmG'CkG
Cuts at: 0 147 351 1623 1710
Size: 147 204 1272 87
Fragments arranged by size:
1272 204 147 87
NspI r_CATG'y
Cuts at: 0 801 1710
Size: 801 909
PasI CC'CwG_GG
Cuts at: 0 1483 1710
Size: 1483 227
PfoI T'CCnGG_A
Cuts at: 0 1612 1710
Size: 1612 98
PmlI CAC'GTG
Cuts at: 0 333 1080 1710
Size: 333 747 630
Fragments arranged by size:
747 630 333
PpuMI rG'GwC_Cy
Cuts at: 0 438 1710
Size: 438 1272
PshAI GACnn'nnGTC
Cuts at: 0 469 1710
Size: 469 1241
PspXI vC'TCGA_Gb
Cuts at: 0 748 1710
Size: 748 962
PstI C_TGCA'G
Cuts at: 0 350 761 930 1710
Size: 350 411 169 780
Fragments arranged by size:
780 411 350 169
PvuII CAG'CTG
Cuts at: 0 147 1623 1710
Size: 147 1476 87
Fragments arranged by size:
1476 147 87
SapI GCTCTTCn'nnn_
Cuts at: 0 92 433 497 1710
Size: 92 341 64 1213
Fragments arranged by size:
1213 341 92 64
SbfI CC_TGCA'GG
Cuts at: 0 761 930 1710
Size: 761 169 780
Fragments arranged by size:
780 761 169
SfcI C'TryA_G
Cuts at: 0 346 757 926 1040 1710
Size: 346 411 169 114 670
Fragments arranged by size:
670 411 346 169 114
SmlI C'TyrA_G
Cuts at: 0 52 748 1710
Size: 52 696 962
Fragments arranged by size:
962 696 52
SphI G_CATG'C
Cuts at: 0 801 1710
Size: 801 909
SspI AAT'ATT
Cuts at: 0 529 1710
Size: 529 1181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 424 961 1118 1496 1710
Size: 424 537 157 378 214
Fragments arranged by size:
537 424 378 214 157
TatI w'GTAC_w
Cuts at: 0 549 671 914 1333 1710
Size: 549 122 243 419 377
Fragments arranged by size:
549 419 377 243 122
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1214 1246 1710
Size: 1214 32 464
Fragments arranged by size:
1214 464 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 565 1620 1710
Size: 565 1055 90
Fragments arranged by size:
1055 565 90
XhoI C'TCGA_G
Cuts at: 0 748 1710
Size: 748 962
Enzymes that do cut:
AcuI AlfI AlwNI ApoI AseI AvaI BamHI BanI
BanII BbsI BclI BglI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI Bsp1286I BsrGI BssSI BstAPI BstYI Bsu36I
BtgI EaeI EarI EciI Eco57MI EcoO109I EcoRI HaeII
Hin4I MmeI MscI MslI MspA1I NspI PasI PfoI
PmlI PpuMI PshAI PspXI PstI PvuII SapI SbfI
SfcI SmlI SphI SspI TaqII TatI TstI XcmI
XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI AscI AsiSI
AvrII BaeI BbeI BbvCI BcgI BciVI BfrBI BglII
BlpI BmgBI BmtI BplI BsaI BsaHI BsaWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BstBI BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EcoICRI EcoNI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PflMI PmeI PpiI
PsiI PspOMI PsrI PvuI RsrII SacI SacII SalI
SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI StuI StyI SwaI Tth111I XbaI
XmaI XmnI ZraI