################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BanI BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BlnI BsaAI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstUI BstZ17I Bsu36I Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I FauI FseI FspI HindIII HpaI KasI KpnI KspI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AfaI 181 GTAC GT^AC AspHI 262 GAGCTC G[AT]GC[AT]^C BalI 557 TGGCCA TGG^CCA BamHI 1052 GGATCC G^GATCC BanII 262 GAGCTC G[GA]GC[CT]^C BbsI 906 GTCTTC GAAGAC(2/6) BclI 269 TGATCA T^GATCA BcnI 920 CCCGG CC^[GC]GG BfaI 124 CTAG C^TAG BglII 875 AGATCT A^GATCT BlpI 598 GCTGAGC GC^T[ACGT]AGC Bpu1102I 598 GCTGAGC GC^T[ACGT]AGC BpuAI 906 GTCTTC GAAGAC(2/6) BsaBI 789 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 847 GAGACC GGTCTC(1/5) BsgI 145 GTGCAG GTGCAG(16/14) BsiHKAI 262 GAGCTC G[AT]GC[AT]^C BspMI 147 GCAGGT ACCTGC(4/8) BsrBI 118 GAGCGG CCGCTC(-3/-3) BsrI 993 CCAGT ACTGG(1/-1) BstAPI 341 GCAAGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 234 TTCGAA TT^CGAA BstEII 957 GGTCACC G^GT[ACGT]ACC BstPI 957 GGTCACC G^GT[ACGT]ACC CelII 598 GCTGAGC GC^T[ACGT]AGC DsaI 686 CCATGG C^C[GA][CT]GG EaeI 557 TGGCCA [CT]^GGCC[GA] EcoNI 1032 CCTTTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 957 GGTCACC G^GT[ACGT]ACC EcoRI 81 GAATTC G^AATTC EcoRV 1018 GATATC GAT^ATC EcoT14I 686 CCATGG C^C[AT][AT]GG EcoT22I 281 ATGCAT ATGCA^T FbaI 269 TGATCA T^GATCA HaeII 602 AGCGCC [GA]GCGC^[CT] HaeIII 558 GGCC GG^CC HapII 921 CCGG C^CGG HpaII 921 CCGG C^CGG MaeI 124 CTAG C^TAG MaeII 242 ACGT A^CGT MamI 789 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 557 TGGCCA TGG^CCA MscI 557 TGGCCA TGG^CCA MspI 921 CCGG C^CGG NciI 920 CCCGG CC^[GC]GG NcoI 686 CCATGG C^CATGG NsiI 281 ATGCAT ATGCA^T NspI 283 GCATGT [GA]CATG^[CT] NspV 234 TTCGAA TT^CGAA PstI 839 CTGCAG CTGCA^G RsaI 181 GTAC GT^AC SacI 262 GAGCTC GAGCT^C SfcI 839 CTGCAG C^T[GA][CT]AG SfuI 234 TTCGAA TT^CGAA SspI 469 AATATT AAT^ATT StyI 686 CCATGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 120 GCGG CCGC(-3/-1) 924 GCGG AcyI 12 GACGCC G[GA]^CG[CT]C 913 GACGCC BsaHI 12 GACGCC G[GA]^CG[CT]C 913 GACGCC BsmI 543 GAATGC GAATGC(1/-1) 675 GCATTC BstXI 251 CCACCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 794 CCATCATCGTGG DdeI 599 CTGAG C^T[ACGT]AG 1098 CTTAG HgaI 12 GACGC GACGC(5/10) 913 GACGC Hin1I 12 GACGCC G[GA]^CG[CT]C 913 GACGCC HincII 478 GTTGAC GT[CT]^[GA]AC 823 GTTGAC HindII 478 GTTGAC GT[CT]^[GA]AC 823 GTTGAC HphI 772 GGTGA GGTGA(8/7) 959 TCACC MaeIII 10 GTGAC ^GT[ACGT]AC 958 GTCAC MseI 487 TTAA T^TAA 1007 TTAA TaqI 79 TCGA T^CGA 235 TCGA TfiI 627 GATTC G^A[AT]TC 738 GATTC Tru9I 487 TTAA T^TAA 1007 TTAA Tsp45I 10 GTGAC ^GT[GC]AC 958 GTCAC TthHB8I 79 TCGA T^CGA 235 TCGA XcmI 47 CCAAAGGTGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 941 CCATTGATGAGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AcsI AluI AlwI ApoI BbvI BmyI BpmI BsaJI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I BstNI BstYI Cac8I CfoI DpnII EarI EcoRII Fnu4HI FokI HhaI HinP1I HinfI ItaI Ksp632I MboI MboII MflI MnlI MslI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI ScrFI SfaNI TseI Tsp509I TspRI XhoII