(Circular) (Six-base) MAPSORT of: pFN21AE3153.seq Check: 9270 from: 1 to: 5812 pFN21AE3153 5812 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3622 278 Size: 53 83 186 936 2086 2468 Fragments arranged by size: 2468 2086 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3155 1937 Size: 1218 4594 Acc65I G'GTAC_C Cuts at: 3133 3133 Size: 5812 AclI AA'CG_TT Cuts at: 1563 3939 4312 1563 Size: 2376 373 3063 Fragments arranged by size: 3063 2376 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2879 3875 5259 5527 1632 Size: 1247 996 1384 268 1917 Fragments arranged by size: 1917 1384 1247 996 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5615 AflIII A'CryG_T Cuts at: 1203 4711 5688 1203 Size: 3508 977 1327 Fragments arranged by size: 3508 1327 977 AgeI A'CCGG_T Cuts at: 4643 4643 Size: 5812 AhdI GACnn_n'nnGTC Cuts at: 4541 4541 Size: 5812 AleI CACnn'nnGTG Cuts at: 1166 2771 1166 Size: 1605 4207 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5812 AlwNI CAG_nnn'CTG Cuts at: 3555 5127 3555 Size: 1572 4240 ApaLI G'TGCA_C Cuts at: 3869 5025 3869 Size: 1156 4656 ApoI r'AATT_y Cuts at: 1493 2086 2190 2457 3121 3347 3499 5608 1493 Size: 593 104 267 664 226 152 2109 1697 Fragments arranged by size: 2109 1697 664 593 267 226 152 104 AseI AT'TA_AT Cuts at: 160 1032 4366 160 Size: 872 3334 1606 Fragments arranged by size: 3334 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5812 AvaI C'yCGr_G Cuts at: 1870 1943 3137 1870 Size: 73 1194 4545 Fragments arranged by size: 4545 1194 73 BamHI G'GATC_C Cuts at: 3057 3142 3509 3057 Size: 85 367 5360 Fragments arranged by size: 5360 367 85 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3133 4588 618 Size: 325 217 627 1346 1455 1842 Fragments arranged by size: 1842 1455 1346 627 325 217 BanII G_rGCy'C Cuts at: 1391 2271 3131 1391 Size: 880 860 4072 Fragments arranged by size: 4072 880 860 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2904 928 Size: 971 1005 3836 Fragments arranged by size: 3836 1005 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4003 4037 1903 Size: 34 2066 34 3678 Fragments arranged by size: 3678 2066 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3704 4914 3704 Size: 1210 4602 BclI T'GATC_A Cuts at: 1548 2274 1548 Size: 726 5086 BfrBI ATG'CAT Cuts at: 2288 2288 Size: 5812 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4423 136 Size: 107 122 71 631 605 2751 1525 Fragments arranged by size: 2751 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2880 5807 2880 Size: 2927 2885 BlpI GC'TnA_GC Cuts at: 2604 3229 2604 Size: 625 5187 Bme1580I G_kGCm'C Cuts at: 1790 2593 3005 3873 5029 1790 Size: 803 412 868 1156 2573 Fragments arranged by size: 2573 1156 868 803 412 BmgBI CAC'GTC Cuts at: 1318 2248 1318 Size: 930 4882 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3543 4501 442 Size: 416 53 83 399 24 2126 958 1753 Fragments arranged by size: 2126 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5812 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2556 2747 2981 4472 1239 Size: 1317 191 234 1491 2579 Fragments arranged by size: 2579 1491 1317 234 191 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3937 4802 5100 3937 Size: 865 298 4649 Fragments arranged by size: 4649 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2846 4475 882 Size: 617 1347 1629 2219 Fragments arranged by size: 2219 1629 1347 617 BsaAI yAC'GTr Cuts at: 493 1204 3603 493 Size: 711 2399 2702 Fragments arranged by size: 2702 2399 711 BsaBI GATnn'nnATC Cuts at: 2798 5806 2798 Size: 3008 2804 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2018 2919 3619 4001 Size: 53 83 186 936 485 901 700 382 Cuts at: 4001 4636 275 Size: 635 1451 Fragments arranged by size: 1451 936 901 700 635 485 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4245 4643 4917 5064 5475 4245 Size: 398 274 147 411 4582 Fragments arranged by size: 4582 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3062 3092 1178 Size: 30 165 30 1659 30 3898 Fragments arranged by size: 3898 1659 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5812 BseYI C'CCAG_C Cuts at: 2720 5015 5511 2720 Size: 2295 496 3021 Fragments arranged by size: 3021 2295 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2171 1163 Size: 573 435 4804 Fragments arranged by size: 4804 573 435 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3262 4023 5051 1540 Size: 275 186 1261 761 1028 2301 Fragments arranged by size: 2301 1261 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2271 3873 3958 5029 2271 Size: 1602 85 1071 3054 Fragments arranged by size: 3054 1602 1071 85 BsmI GAATG_Cn' Cuts at: 2554 2680 3323 3416 2554 Size: 126 643 93 4950 Fragments arranged by size: 4950 643 126 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2271 2593 3005 3131 3873 3958 5029 Size: 399 481 322 412 126 742 85 1071 Cuts at: 5029 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 481 412 399 322 126 85 BspEI T'CCGG_A Cuts at: 5475 5475 Size: 5812 BspHI T'CATG_A Cuts at: 3701 5431 3701 Size: 1730 4082 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2143 3167 844 Size: 1299 1024 3489 Fragments arranged by size: 3489 1299 1024 BsrBI CCG'CTC Cuts at: 1394 1823 2125 3699 1394 Size: 429 302 1574 3507 Fragments arranged by size: 3507 1574 429 302 BsrDI GCAATG_nn' Cuts at: 65 1248 4307 4481 65 Size: 1183 3059 174 1396 Fragments arranged by size: 3059 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4456 4643 1700 Size: 110 2646 187 2869 Fragments arranged by size: 2869 2646 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5812 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5812 BssSI C'ACGA_G Cuts at: 3872 4884 3872 Size: 1012 4800 BstAPI GCAn_nnn'nTGC Cuts at: 794 2352 794 Size: 1558 4254 BstBI TT'CG_AA Cuts at: 2240 4616 2240 Size: 2376 3436 BstEII G'GTnAC_C Cuts at: 1184 2962 1184 Size: 1778 4034 BstXI CCAn_nnnn'nTGG Cuts at: 2263 2806 2263 Size: 543 5269 BstYI r'GATC_y Cuts at: 1972 2532 2730 2880 3057 3142 3509 3894 3911 Size: 560 198 150 177 85 367 385 17 Cuts at: 3911 5363 5449 5461 5807 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 560 385 367 346 198 177 150 86 85 17 12 BtgI C'CryG_G Cuts at: 513 1065 2691 513 Size: 552 1626 3634 Fragments arranged by size: 3634 1626 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5073 Fragments arranged by size: 5073 683 56 BtsI GCAGTG_nn' Cuts at: 2357 2634 3328 4122 4142 2357 Size: 277 694 794 20 4027 Fragments arranged by size: 4027 794 694 277 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5812 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5777 DraI TTT'AAA Cuts at: 3116 3116 Size: 5812 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4819 809 Size: 507 3503 1802 Fragments arranged by size: 3503 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2562 3259 4148 Size: 54 1425 50 275 18 732 697 889 Cuts at: 4148 5777 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 732 697 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3259 1537 Size: 275 1447 4090 Fragments arranged by size: 4090 1447 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2523 2579 2931 3742 1353 Size: 1170 56 352 811 3423 Fragments arranged by size: 3423 1170 811 352 56 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2944 4399 4773 4919 387 Size: 1064 138 1355 1455 374 146 1280 Fragments arranged by size: 1455 1355 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2269 2269 Size: 5812 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2556 2747 2879 2981 3875 4472 5259 Size: 393 924 191 132 102 894 597 787 Cuts at: 5259 5527 1239 Size: 268 1524 Fragments arranged by size: 1524 924 894 787 597 393 268 191 132 102 EcoNI CCTnn'n_nnAGG Cuts at: 3041 3041 Size: 5812 EcoRI G'AATT_C Cuts at: 2086 3121 3499 2086 Size: 1035 378 4399 Fragments arranged by size: 4399 1035 378 EcoRV GAT'ATC Cuts at: 3025 3025 Size: 5812 FspI TGC'GCA Cuts at: 4318 4318 Size: 5812 HaeII r_GCGC'y Cuts at: 1678 2611 4959 1678 Size: 933 2348 2531 Fragments arranged by size: 2531 2348 933 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2642 2674 2881 2913 3062 Size: 32 135 32 1070 32 207 32 149 Cuts at: 3062 3094 4453 4485 4527 4559 1373 Size: 32 1359 32 42 32 2626 Fragments arranged by size: 2626 1359 1070 207 149 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2485 2830 3156 3402 669 Size: 988 281 547 345 326 246 3079 Fragments arranged by size: 3079 988 547 345 326 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5812 HpaI GTT'AAC Cuts at: 3402 3402 Size: 5812 KpnI G_GTAC'C Cuts at: 3137 3137 Size: 5812 MfeI C'AATT_G Cuts at: 3411 3411 Size: 5812 MluI A'CGCG_T Cuts at: 5688 5688 Size: 5812 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2640 2764 3035 4926 5110 1305 Size: 1335 124 271 1891 184 2007 Fragments arranged by size: 2007 1891 1335 271 184 124 MscI TGG'CCA Cuts at: 10 64 1489 1832 2564 10 Size: 54 1425 343 732 3258 Fragments arranged by size: 3258 1425 732 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2261 2321 2771 2804 3770 4129 4288 Size: 648 1095 60 450 33 966 359 159 Cuts at: 4288 518 Size: 2042 Fragments arranged by size: 2042 1095 966 648 450 359 159 60 33 MspA1I CmG'CkG Cuts at: 1606 3228 3905 5053 5298 1606 Size: 1622 677 1148 245 2120 Fragments arranged by size: 2120 1622 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2691 513 Size: 552 1626 3634 Fragments arranged by size: 3634 1626 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5812 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5812 NotI GC'GGCC_GC Cuts at: 3259 3259 Size: 5812 NsiI A_TGCA'T Cuts at: 2290 2290 Size: 5812 NspI r_CATG'y Cuts at: 1132 1229 2292 3170 4715 1132 Size: 97 1063 878 1545 2229 Fragments arranged by size: 2229 1545 1063 878 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5812 PciI A'CATG_T Cuts at: 4711 4711 Size: 5812 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5812 PmeI GTTT'AAAC Cuts at: 3116 3116 Size: 5812 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3881 3913 5394 5426 3881 Size: 32 1481 32 4267 Fragments arranged by size: 4267 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5812 PsiI TTA'TAA Cuts at: 3382 3382 Size: 5812 PstI C_TGCA'G Cuts at: 830 2848 3164 830 Size: 2018 316 3478 Fragments arranged by size: 3478 2018 316 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5812 SacI G_AGCT'C Cuts at: 2271 2271 Size: 5812 SalI G'TCGA_C Cuts at: 1936 3154 1936 Size: 1218 4594 SbfI CC_TGCA'GG Cuts at: 3164 3164 Size: 5812 ScaI AGT'ACT Cuts at: 4060 5772 4060 Size: 1712 4100 SfcI C'TryA_G Cuts at: 826 1045 2844 3160 4295 4976 5167 826 Size: 219 1799 316 1135 681 191 1471 Fragments arranged by size: 1799 1471 1135 681 316 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5812 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5812 SmaI CCC'GGG Cuts at: 1872 3139 1872 Size: 1267 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3916 4817 5079 820 Size: 197 926 1973 901 262 1553 Fragments arranged by size: 1973 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3603 493 Size: 3110 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5812 SphI G_CATG'C Cuts at: 1132 3170 1132 Size: 2038 3774 SspI AAT'ATT Cuts at: 5 52 2476 3736 5 Size: 47 2424 1260 2081 Fragments arranged by size: 2424 2081 1260 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5812 StyI C'CwwG_G Cuts at: 513 1065 2691 3251 513 Size: 552 1626 560 3074 Fragments arranged by size: 3074 1626 560 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2704 2765 3839 3856 4009 4194 4639 Size: 1255 1124 61 1074 17 153 185 445 Cuts at: 4639 325 Size: 1498 Fragments arranged by size: 1498 1255 1124 1074 445 185 153 61 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2185 4058 5770 Size: 274 80 33 51 1443 208 1873 1712 Cuts at: 5770 96 Size: 138 Fragments arranged by size: 1873 1712 1443 274 208 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2613 2645 2970 3002 5772 5804 2613 Size: 32 325 32 2770 32 2621 Fragments arranged by size: 2770 2621 325 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5812 XbaI T'CTAG_A Cuts at: 3148 3148 Size: 5812 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2059 2953 2059 Size: 894 4918 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5812 XmaI C'CCGG_G Cuts at: 1870 3137 1870 Size: 1267 4545 XmnI GAAnn'nnTTC Cuts at: 3941 3941 Size: 5812 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3620 276 Size: 53 83 186 936 2086 2468 Fragments arranged by size: 2468 2086 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI