################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII BalI BanI BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BpuAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HinfI HpaI KasI KpnI MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcyI 409 GGCGTC G[GA]^CG[CT]C AfaI 537 GTAC GT^AC AhdI 404 GACTGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 50 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 404 GACTGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 18 CCCGGG C^[CT]CG[GA]G AvrII 462 CCTAGG C^CTAGG BanII 652 GAGCCC G[GA]GC[CT]^C BcgI 239 GCAGGGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 463 CTAG C^TAG BglI 587 GCCCCACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 462 CCTAGG C^CTAGG BpmI 426 CTCCAG CTGGAG(16/14) BsaAI 130 TACGTG [CT]AC^GT[GA] BsaHI 409 GGCGTC G[GA]^CG[CT]C BseRI 702 GAGGAG GAGGAG(10/8) BsoBI 18 CCCGGG C^[CT]CG[GA]G BsrBI 187 CCGCTC CCGCTC(-3/-3) BsrFI 674 ACCGGC [GA]^CCGG[CT] BssSI 418 CTCGTG CACGAG(-5/-1) BstAPI 327 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 674 ACCGGC [GA]^CCGG[CT] Eam1105I 404 GACTGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 483 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 462 CCTAGG C^C[AT][AT]GG HgaI 410 GCGTC GACGC(5/10) Hin1I 409 GGCGTC G[GA]^CG[CT]C KspI 371 CCGCGG CCGC^GG MaeI 463 CTAG C^TAG MaeII 131 ACGT A^CGT PflMI 533 CCATGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 691 GGGTCCC [GA]G^G[AT]CC[CT] RsaI 537 GTAC GT^AC SacII 371 CCGCGG CCGC^GG SapI 649 GAAGAGC GCTCTTC(1/4) SfaNI 281 GCATC GCATC(5/9) SfcI 378 CTACAG C^T[GA][CT]AG SmaI 18 CCCGGG CCC^GGG SmlI 484 CTCAAG C^T[CT][GA]AG SspI 79 AATATT AAT^ATT StuI 69 AGGCCT AGG^CCT StyI 462 CCTAGG C^C[AT][AT]GG Tsp45I 499 GTGAC ^GT[GC]AC Van91I 533 CCATGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 18 CCCGGG C^CCGGG XmaI 18 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 372 CGCG CG^CG 393 CGCG AspHI 415 GTGCTC G[AT]GC[AT]^C 421 GTGCTC AvaII 135 GGACC G^G[AT]CC 692 GGTCC BglII 7 AGATCT A^GATCT 668 AGATCT BlpI 234 GCTGAGC GC^T[ACGT]AGC 336 GCTGAGC Bpu1102I 234 GCTGAGC GC^T[ACGT]AGC 336 GCTGAGC BsiHKAI 415 GTGCTC G[AT]GC[AT]^C 421 GTGCTC BsmFI 402 GGGAC GGGAC(10/14) 693 GTCCC BstUI 372 CGCG CG^CG 393 CGCG BstXI 149 CCAGCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 554 CCAACTACCTGG CelII 234 GCTGAGC GC^T[ACGT]AGC 336 GCTGAGC CfoI 309 GCGC GCG^C 394 GCGC DsaI 371 CCGCGG C^C[GA][CT]GG 590 CCACGG EarI 3 GAAGAG CTCTTC(1/4) 649 GAAGAG FauI 227 CCCGC CCCGC(4/6) 370 CCCGC HhaI 309 GCGC GCG^C 394 GCGC HinP1I 309 GCGC G^CGC 394 GCGC Ksp632I 3 GAAGAG CTCTTC(1/4) 649 GAAGAG MaeIII 31 GTAAC ^GT[ACGT]AC 499 GTGAC MboII 3 GAAGA GAAGA(8/7) 649 GAAGA MspA1I 371 CCGCGG C[AC]G^C[GT]G 662 CCGCTG MvnI 372 CGCG CG^CG 393 CGCG TspRI 316 CACTG CA[GC]TG[ACGT][ACGT]^ 440 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AluI AlwI BamHI BbvI BcnI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MboI MflI MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI XhoII