(Circular) (Six-base) MAPSORT of: pFN21AE1728.seq Check: 4387 from: 1 to: 5413 pFN21AE1728 5413 bp With 182 enzymes: SgfI * July 6, 2012 17:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3223 278 Size: 53 83 186 936 1687 2468 Fragments arranged by size: 2468 1687 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2756 1937 Size: 819 4594 Acc65I G'GTAC_C Cuts at: 2734 2734 Size: 5413 AclI AA'CG_TT Cuts at: 1563 3540 3913 1563 Size: 1977 373 3063 Fragments arranged by size: 3063 1977 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3476 4860 5128 1632 Size: 1844 1384 268 1917 Fragments arranged by size: 1917 1844 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5216 AflIII A'CryG_T Cuts at: 1203 4312 5289 1203 Size: 3109 977 1327 Fragments arranged by size: 3109 1327 977 AgeI A'CCGG_T Cuts at: 4244 4244 Size: 5413 AhdI GACnn_n'nnGTC Cuts at: 2414 4142 2414 Size: 1728 3685 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5413 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2125 1184 Size: 941 4472 AlwNI CAG_nnn'CTG Cuts at: 2060 3156 4728 2060 Size: 1096 1572 2745 Fragments arranged by size: 2745 1572 1096 ApaLI G'TGCA_C Cuts at: 3470 4626 3470 Size: 1156 4257 ApoI r'AATT_y Cuts at: 1493 2722 2948 3100 5209 1493 Size: 1229 226 152 2109 1697 Fragments arranged by size: 2109 1697 1229 226 152 AseI AT'TA_AT Cuts at: 160 1032 3967 160 Size: 872 2935 1606 Fragments arranged by size: 2935 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5413 AvaI C'yCGr_G Cuts at: 1870 1943 2023 2738 1870 Size: 73 80 715 4545 Fragments arranged by size: 4545 715 80 73 AvrII C'CTAG_G Cuts at: 2467 2467 Size: 5413 BamHI G'GATC_C Cuts at: 2149 2255 2484 2743 3110 2149 Size: 106 229 259 367 4452 Fragments arranged by size: 4452 367 259 229 106 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2734 4189 618 Size: 325 217 627 947 1455 1842 Fragments arranged by size: 1842 1455 947 627 325 217 BanII G_rGCy'C Cuts at: 1391 2661 2732 1391 Size: 1270 71 4072 Fragments arranged by size: 4072 1270 71 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4442 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2233 2267 3604 3638 1903 Size: 34 296 34 1337 34 3678 Fragments arranged by size: 3678 1337 296 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3305 4515 3305 Size: 1210 4203 BclI T'GATC_A Cuts at: 1548 1548 Size: 5413 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2598 4024 136 Size: 107 122 71 631 605 926 1426 1525 Fragments arranged by size: 1525 1426 926 631 605 122 107 71 BglII A'GATC_T Cuts at: 2012 2673 5408 2012 Size: 661 2735 2017 Fragments arranged by size: 2735 2017 661 BlpI GC'TnA_GC Cuts at: 2240 2342 2830 2240 Size: 102 488 4823 Fragments arranged by size: 4823 488 102 Bme1580I G_kGCm'C Cuts at: 1790 3474 4630 1790 Size: 1684 1156 2573 Fragments arranged by size: 2573 1684 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5413 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2420 2456 2554 Size: 416 53 83 399 24 1003 36 98 Cuts at: 2554 3144 4102 442 Size: 590 958 1753 Fragments arranged by size: 1753 1003 958 590 416 399 98 83 53 36 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5413 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2416 4073 1239 Size: 1177 1657 2579 Fragments arranged by size: 2579 1657 1177 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2474 3538 4403 4701 2474 Size: 1064 865 298 3186 Fragments arranged by size: 3186 1064 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4076 882 Size: 617 2577 2219 Fragments arranged by size: 2577 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2137 3204 493 Size: 711 933 1067 2702 Fragments arranged by size: 2702 1067 933 711 BsaBI GATnn'nnATC Cuts at: 5407 5407 Size: 5413 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2415 3220 3602 4237 Size: 53 83 186 936 882 805 382 635 Cuts at: 4237 275 Size: 1451 Fragments arranged by size: 1451 936 882 805 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3846 4244 4518 4665 5076 3846 Size: 398 274 147 411 4183 Fragments arranged by size: 4183 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2019 2049 1178 Size: 30 165 30 616 30 4542 Fragments arranged by size: 4542 616 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2722 1186 Size: 1536 3877 BseYI C'CCAG_C Cuts at: 2128 2153 2571 4616 5112 2128 Size: 25 418 2045 496 2429 Fragments arranged by size: 2429 2045 496 418 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4840 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2863 3624 4652 1540 Size: 275 186 862 761 1028 2301 Fragments arranged by size: 2301 1028 862 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2424 2430 3474 3559 4630 2424 Size: 6 1044 85 1071 3207 Fragments arranged by size: 3207 1071 1044 85 6 BsmI GAATG_Cn' Cuts at: 2924 3017 2924 Size: 93 5320 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2424 2430 2661 2732 3474 3559 4630 Size: 399 634 6 231 71 742 85 1071 Cuts at: 4630 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 634 399 231 85 71 6 BspEI T'CCGG_A Cuts at: 5076 5076 Size: 5413 BspHI T'CATG_A Cuts at: 3302 5032 3302 Size: 1730 3683 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2768 844 Size: 1924 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2194 3300 1394 Size: 429 371 1106 3507 Fragments arranged by size: 3507 1106 429 371 BsrDI GCAATG_nn' Cuts at: 65 1248 3908 4082 65 Size: 1183 2660 174 1396 Fragments arranged by size: 2660 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2679 4057 4244 1700 Size: 110 869 1378 187 2869 Fragments arranged by size: 2869 1378 869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5413 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5413 BssSI C'ACGA_G Cuts at: 2423 3473 4485 2423 Size: 1050 1012 3351 Fragments arranged by size: 3351 1050 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2338 794 Size: 1544 3869 BstBI TT'CG_AA Cuts at: 4217 4217 Size: 5413 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5413 BstXI CCAn_nnnn'nTGG Cuts at: 2161 2566 2161 Size: 405 5008 BstYI r'GATC_y Cuts at: 1972 2012 2149 2255 2484 2673 2743 3110 3495 Size: 40 137 106 229 189 70 367 385 Cuts at: 3495 3512 4964 5050 5062 5408 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 229 189 137 106 86 70 40 17 12 BtgI C'CryG_G Cuts at: 513 1065 2376 2595 513 Size: 552 1311 219 3331 Fragments arranged by size: 3331 1311 552 219 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4674 Fragments arranged by size: 4674 683 56 BtsI GCAGTG_nn' Cuts at: 2320 2929 3723 3743 2320 Size: 609 794 20 3990 Fragments arranged by size: 3990 794 609 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5413 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5378 DraI TTT'AAA Cuts at: 2717 2717 Size: 5413 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4420 809 Size: 507 3104 1802 Fragments arranged by size: 3104 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2860 3749 5378 Size: 54 1425 50 275 18 1030 889 1629 Cuts at: 5378 8 Size: 43 Fragments arranged by size: 1629 1425 1030 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2860 1537 Size: 275 1048 4090 Fragments arranged by size: 4090 1048 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2003 2649 3343 1353 Size: 650 646 694 3423 Fragments arranged by size: 3423 694 650 646 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4000 4374 4520 387 Size: 1064 138 2411 374 146 1280 Fragments arranged by size: 2411 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2416 3476 4073 4860 5128 1239 Size: 393 784 1060 597 787 268 1524 Fragments arranged by size: 1524 1060 787 784 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2492 2492 Size: 5413 EcoO109I rG'GnC_Cy Cuts at: 2057 2247 2697 2057 Size: 190 450 4773 Fragments arranged by size: 4773 450 190 EcoRI G'AATT_C Cuts at: 2722 3100 2722 Size: 378 5035 FspI TGC'GCA Cuts at: 3919 3919 Size: 5413 HaeII r_GCGC'y Cuts at: 1678 4560 1678 Size: 2882 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2400 2432 4054 4086 4128 Size: 32 135 32 828 32 1622 32 42 Cuts at: 4128 4160 1373 Size: 32 2626 Fragments arranged by size: 2626 1622 828 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2757 3003 669 Size: 988 281 819 246 3079 Fragments arranged by size: 3079 988 819 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5413 HpaI GTT'AAC Cuts at: 3003 3003 Size: 5413 KpnI G_GTAC'C Cuts at: 2738 2738 Size: 5413 MfeI C'AATT_G Cuts at: 3012 3012 Size: 5413 MluI A'CGCG_T Cuts at: 5289 5289 Size: 5413 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4527 4711 1305 Size: 3222 184 2007 Fragments arranged by size: 3222 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3591 Fragments arranged by size: 3591 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3371 3730 3889 518 Size: 648 2205 359 159 2042 Fragments arranged by size: 2205 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2378 2669 2829 3506 4654 4899 1606 Size: 772 291 160 677 1148 245 2120 Fragments arranged by size: 2120 1148 772 677 291 245 160 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4861 NdeI CA'TA_TG Cuts at: 387 387 Size: 5413 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5413 NotI GC'GGCC_GC Cuts at: 2860 2860 Size: 5413 NspI r_CATG'y Cuts at: 1132 1229 2771 4316 1132 Size: 97 1542 1545 2229 Fragments arranged by size: 2229 1545 1542 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5413 PciI A'CATG_T Cuts at: 4312 4312 Size: 5413 PflMI CCAn_nnn'nTGG Cuts at: 1108 2544 1108 Size: 1436 3977 PfoI T'CCnGG_A Cuts at: 2526 2526 Size: 5413 PmeI GTTT'AAAC Cuts at: 2717 2717 Size: 5413 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3482 3514 4995 5027 3482 Size: 32 1481 32 3868 Fragments arranged by size: 3868 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2697 2697 Size: 5413 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5413 PsiI TTA'TAA Cuts at: 2983 2983 Size: 5413 PstI C_TGCA'G Cuts at: 830 2765 830 Size: 1935 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5413 SacII CC_GC'GG Cuts at: 2379 2379 Size: 5413 SalI G'TCGA_C Cuts at: 1936 2755 1936 Size: 819 4594 SanDI GG'GwC_CC Cuts at: 2697 2697 Size: 5413 SapI GCTCTTCn'nnn_ Cuts at: 2649 2649 Size: 5413 SbfI CC_TGCA'GG Cuts at: 2765 2765 Size: 5413 ScaI AGT'ACT Cuts at: 3661 5373 3661 Size: 1712 3701 SfcI C'TryA_G Cuts at: 826 1045 2383 2761 3896 4577 4768 826 Size: 219 1338 378 1135 681 191 1471 Fragments arranged by size: 1471 1338 1135 681 378 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5413 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5413 SmaI CCC'GGG Cuts at: 1872 2025 2740 1872 Size: 153 715 4545 Fragments arranged by size: 4545 715 153 SmlI C'TyrA_G Cuts at: 820 1017 1943 2489 3517 4418 4680 820 Size: 197 926 546 1028 901 262 1553 Fragments arranged by size: 1553 1028 926 901 546 262 197 SnaBI TAC'GTA Cuts at: 493 3204 493 Size: 2711 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5413 SphI G_CATG'C Cuts at: 1132 2771 1132 Size: 1639 3774 SspI AAT'ATT Cuts at: 5 52 2086 3337 5 Size: 47 2034 1251 2081 Fragments arranged by size: 2081 2034 1251 47 StuI AGG'CCT Cuts at: 1517 2076 1517 Size: 559 4854 StyI C'CwwG_G Cuts at: 513 1065 2467 2852 513 Size: 552 1402 385 3074 Fragments arranged by size: 3074 1402 552 385 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3440 3457 3610 3795 4240 325 Size: 1255 1860 17 153 185 445 1498 Fragments arranged by size: 1860 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2541 3659 5371 Size: 274 80 33 51 1443 564 1118 1712 Cuts at: 5371 96 Size: 138 Fragments arranged by size: 1712 1443 1118 564 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2173 2205 5373 5405 2173 Size: 32 3168 32 2181 Fragments arranged by size: 3168 2181 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5413 XbaI T'CTAG_A Cuts at: 2749 2749 Size: 5413 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5413 XmaI C'CCGG_G Cuts at: 1870 2023 2738 1870 Size: 153 715 4545 Fragments arranged by size: 4545 715 153 XmnI GAAnn'nnTTC Cuts at: 3542 3542 Size: 5413 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3221 276 Size: 53 83 186 936 1687 2468 Fragments arranged by size: 2468 1687 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SexAI SfiI SfoI SrfI SwaI XcmI