(Linear) (Six-base) MAPSORT of: KIEE1728.seq Check: 5288 from: 1 to: 708 KIEE1728.orf With 182 enzymes: SgfI * July 3, 2012 17:00 .. AhdI GACnn_n'nnGTC Cuts at: 0 409 708 Size: 409 299 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 120 708 Size: 120 588 AlwNI CAG_nnn'CTG Cuts at: 0 55 708 Size: 55 653 AvaI C'yCGr_G Cuts at: 0 18 708 Size: 18 690 AvrII C'CTAG_G Cuts at: 0 462 708 Size: 462 246 BamHI G'GATC_C Cuts at: 0 144 250 479 708 Size: 144 106 229 229 Fragments arranged by size: 229 229 144 106 BanII G_rGCy'C Cuts at: 0 656 708 Size: 656 52 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 228 262 708 Size: 228 34 446 Fragments arranged by size: 446 228 34 BglI GCCn_nnn'nGGC Cuts at: 0 593 708 Size: 593 115 BglII A'GATC_T Cuts at: 0 7 668 708 Size: 7 661 40 Fragments arranged by size: 661 40 7 BlpI GC'TnA_GC Cuts at: 0 235 337 708 Size: 235 102 371 Fragments arranged by size: 371 235 102 BmrI ACTGGGnnnn_n' Cuts at: 0 415 451 549 708 Size: 415 36 98 159 Fragments arranged by size: 415 159 98 36 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 411 708 Size: 411 297 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 469 708 Size: 469 239 BsaAI yAC'GTr Cuts at: 0 132 708 Size: 132 576 BsaHI Gr'CG_yC Cuts at: 0 410 708 Size: 410 298 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 14 44 708 Size: 14 30 664 Fragments arranged by size: 664 30 14 BseYI C'CCAG_C Cuts at: 0 123 148 566 708 Size: 123 25 418 142 Fragments arranged by size: 418 142 123 25 BsiHKAI G_wGCw'C Cuts at: 0 419 425 708 Size: 419 6 283 Fragments arranged by size: 419 283 6 Bsp1286I G_dGCh'C Cuts at: 0 419 425 656 708 Size: 419 6 231 52 Fragments arranged by size: 419 231 52 6 BsrBI CCG'CTC Cuts at: 0 189 708 Size: 189 519 BsrFI r'CCGG_y Cuts at: 0 674 708 Size: 674 34 BssSI C'ACGA_G Cuts at: 0 418 708 Size: 418 290 BstAPI GCAn_nnn'nTGC Cuts at: 0 333 708 Size: 333 375 BstXI CCAn_nnnn'nTGG Cuts at: 0 156 561 708 Size: 156 405 147 Fragments arranged by size: 405 156 147 BstYI r'GATC_y Cuts at: 0 7 144 250 479 668 708 Size: 7 137 106 229 189 40 Fragments arranged by size: 229 189 137 106 40 7 BtgI C'CryG_G Cuts at: 0 371 590 708 Size: 371 219 118 Fragments arranged by size: 371 219 118 BtsI GCAGTG_nn' Cuts at: 0 315 708 Size: 315 393 EarI CTCTTCn'nnn_ Cuts at: 0 644 708 Size: 644 64 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 411 708 Size: 411 297 EcoNI CCTnn'n_nnAGG Cuts at: 0 487 708 Size: 487 221 EcoO109I rG'GnC_Cy Cuts at: 0 52 242 692 708 Size: 52 190 450 16 Fragments arranged by size: 450 190 52 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 395 427 708 Size: 395 32 281 Fragments arranged by size: 395 281 32 MspA1I CmG'CkG Cuts at: 0 373 664 708 Size: 373 291 44 Fragments arranged by size: 373 291 44 PflMI CCAn_nnn'nTGG Cuts at: 0 539 708 Size: 539 169 PfoI T'CCnGG_A Cuts at: 0 521 708 Size: 521 187 PpuMI rG'GwC_Cy Cuts at: 0 692 708 Size: 692 16 SacII CC_GC'GG Cuts at: 0 374 708 Size: 374 334 SanDI GG'GwC_CC Cuts at: 0 692 708 Size: 692 16 SapI GCTCTTCn'nnn_ Cuts at: 0 644 708 Size: 644 64 SfcI C'TryA_G Cuts at: 0 378 708 Size: 378 330 SmaI CCC'GGG Cuts at: 0 20 708 Size: 20 688 SmlI C'TyrA_G Cuts at: 0 484 708 Size: 484 224 SspI AAT'ATT Cuts at: 0 81 708 Size: 81 627 StuI AGG'CCT Cuts at: 0 71 708 Size: 71 637 StyI C'CwwG_G Cuts at: 0 462 708 Size: 462 246 TatI w'GTAC_w Cuts at: 0 536 708 Size: 536 172 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 168 200 708 Size: 168 32 508 Fragments arranged by size: 508 168 32 XmaI C'CCGG_G Cuts at: 0 18 708 Size: 18 690 Enzymes that do cut: AhdI AlfI AlwNI AvaI AvrII BamHI BanII BcgI BglI BglII BlpI BmrI BpmI BpuEI BsaAI BsaHI BsaXI BseYI BsiHKAI Bsp1286I BsrBI BsrFI BssSI BstAPI BstXI BstYI BtgI BtsI EarI Eco57MI EcoNI EcoO109I Hin4I MspA1I PflMI PfoI PpuMI SacII SanDI SapI SfcI SmaI SmlI SspI StuI StyI TatI TstI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI BaeI BanI BbeI BbsI BbvCI BciVI BclI BfrBI Bme1580I BmgBI BmtI BplI Bpu10I BsaI BsaBI BsaWI BseRI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI TaqII Tth111I XbaI XcmI XhoI XmnI ZraI