################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvrII BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AlwI 585 GGATC GGATC(4/5) AocI 198 CCTAAGG CC^T[ACGT]AGG ApaI 494 GGGCCC GGGCC^C AvaII 125 GGACC G^G[AT]CC AviII 563 TGCGCA TGC^GCA BanII 494 GGGCCC G[GA]GC[CT]^C BbrPI 376 CACGTG CAC^GTG BcnI 722 CCCGG CC^[GC]GG BfaI 184 CTAG C^TAG BpmI 309 CTCCAG CTGGAG(16/14) BsaAI 376 CACGTG [CT]AC^GT[GA] BsaJI 351 CCCAGG C^C[ACGT][ACGT]GG BsgI 499 CTGCAC GTGCAG(16/14) BspMI 215 ACCTGC ACCTGC(4/8) BstF5I 615 GGATG GGATG(2/0) BstNI 352 CCAGG CC^[AT]GG BstYI 585 GGATCT [GA]^GATC[CT] Bsu36I 198 CCTAAGG CC^T[ACGT]AGG DpnII 586 GATC ^GATC DraIII 205 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 198 CCTAAGG CC^T[ACGT]AGG EcoRII 352 CCAGG ^CC[AT]GG FauI 194 CCCGC CCCGC(4/6) FokI 615 GGATG GGATG(9/13) FspI 563 TGCGCA TGC^GCA HphI 429 GGTGA GGTGA(8/7) MaeI 184 CTAG C^TAG MaeII 377 ACGT A^CGT MboI 586 GATC ^GATC MflI 585 GGATCT [GA]^GATC[CT] MslI 402 CATAGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 485 CAGCTG C[AC]G^C[GT]G MvaI 352 CCAGG CC^[AT]GG NciI 722 CCCGG CC^[GC]GG NdeII 586 GATC ^GATC NspI 356 GCATGC [GA]CATG^[CT] PmaCI 376 CACGTG CAC^GTG PmlI 376 CACGTG CAC^GTG PstI 230 CTGCAG CTGCA^G PvuII 485 CAGCTG CAG^CTG Sau3AI 586 GATC ^GATC SfcI 230 CTGCAG C^T[GA][CT]AG SphI 356 GCATGC GCATG^C TaqI 437 TCGA T^CGA Tsp45I 430 GTGAC ^GT[GC]AC Tsp509I 238 AATT ^AATT TthHB8I 437 TCGA T^CGA XcmI 352 CCAGGCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 585 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### BalI 380 TGGCCA TGG^CCA 456 TGGCCA BseRI 69 CTCCTC GAGGAG(10/8) 688 CTCCTC BsmAI 256 GAGAC GTCTC(1/5) 597 GTCTC BsmFI 65 GGGAC GGGAC(10/14) 365 GGGAC BsrDI 471 CATTGC GCAATG(2/0) 7 GCAATG BsrI 206 ACTGG ACTGG(1/-1) 311 CCAGT BssKI 352 CCAGG ^CC[ACGT]GG 722 CCCGG CfoI 142 GCGC GCG^C 564 GCGC DraII 493 AGGGCCC [GA]G^G[ACGT]CC[CT] 494 GGGCCCT EaeI 380 TGGCCA [CT]^GGCC[GA] 456 TGGCCA EarI 622 CTCTTC CTCTTC(1/4) 84 GAAGAG EcoO109I 493 AGGGCCC [GA]G^G[ACGT]CC[CT] 494 GGGCCCT HapII 123 CCGG C^CGG 723 CCGG HhaI 142 GCGC GCG^C 564 GCGC HinP1I 142 GCGC G^CGC 564 GCGC HpaII 123 CCGG C^CGG 723 CCGG Ksp632I 622 CTCTTC CTCTTC(1/4) 84 GAAGAG MaeIII 430 GTGAC ^GT[ACGT]AC 516 GTTAC MluNI 380 TGGCCA TGG^CCA 456 TGGCCA MscI 380 TGGCCA TGG^CCA 456 TGGCCA MspI 123 CCGG C^CGG 723 CCGG ScrFI 352 CCAGG CC^[ACGT]GG 722 CCCGG SfaNI 439 GATGC GCATC(5/9) 416 GCATC TfiI 300 GATTC G^A[AT]TC 734 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AciI AfaI AluI AspHI BbvI BmyI BsiHKAI BsiYI BslI Bsp1286I Cac8I Cfr13I DdeI Fnu4HI HaeIII HinfI ItaI MboII MnlI MwoI NlaIII NlaIV PleI RsaI Sau96I TseI TspRI