################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaJI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoT14I FauI FbaI FseI FspI Hin1I KasI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tsp45I Tth111I Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 718 GGTACC G^GTACC AccII 995 CGCG CG^CG AciI 623 CCGC CCGC(-3/-1) AlwI 771 GGATC GGATC(4/5) Asp718I 718 GGTACC G^GTACC AvaII 834 GGACC G^G[AT]CC BanI 718 GGTACC G^G[CT][GA]CC BanII 987 GGGCTC G[GA]GC[CT]^C BbsI 409 GAAGAC GAAGAC(2/6) BbvI 811 GCAGC GCAGC(8/12) BcnI 1012 CCGGG CC^[GC]GG BglI 382 GCCAAAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 987 GGGCTC G[AGT]GC[ACT]^C BpuAI 409 GAAGAC GAAGAC(2/6) Bsp1286I 987 GGGCTC G[AGT]GC[ACT]^C Bsp1407I 498 TGTACA T^GTACA BspMI 276 GCAGGT ACCTGC(4/8) BsrDI 893 GCAATG GCAATG(2/0) BsrGI 498 TGTACA T^GTACA BstUI 995 CGCG CG^CG BstYI 771 GGATCT [GA]^GATC[CT] CfoI 1009 GCGC GCG^C DraI 33 TTTAAA TTT^AAA DrdI 619 GACTCCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 737 CCTCTGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 115 GAATTC G^AATTC EcoRV 747 GATATC GAT^ATC EcoT22I 449 ATGCAT ATGCA^T Fnu4HI 811 GCAGC GC^[ACGT]GC HaeII 1008 AGCGCC [GA]GCGC^[CT] HapII 1012 CCGG C^CGG HgaI 996 GCGTC GACGC(5/10) HhaI 1009 GCGC GCG^C HinP1I 1009 GCGC G^CGC HincII 871 GTTAAC GT[CT]^[GA]AC HindII 871 GTTAAC GT[CT]^[GA]AC HindIII 84 AAGCTT A^AGCTT HpaI 871 GTTAAC GTT^AAC HpaII 1012 CCGG C^CGG ItaI 811 GCAGC GC^[ACGT]GC KpnI 718 GGTACC GGTAC^C MaeIII 568 GTAAC ^GT[ACGT]AC MflI 771 GGATCT [GA]^GATC[CT] MspI 1012 CCGG C^CGG MvnI 995 CGCG CG^CG NciI 1012 CCGGG CC^[GC]GG NsiI 449 ATGCAT ATGCA^T SspBI 498 TGTACA T^GTACA TseI 811 GCAGC G^C[AT]GC XbaI 600 TCTAGA T^CTAGA XcmI 362 CCAACGACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 771 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 115 GAATTC [GA]^AATT[CT] 822 GAATTT ApoI 115 GAATTC [GA]^AATT[CT] 822 GAATTT AvaI 98 CTCGGG C^[CT]CG[GA]G 311 CTCGGG BpmI 352 CTGGAG CTGGAG(16/14) 373 CTGGAG BsaI 279 GGTCTC GGTCTC(1/5) 492 GGTCTC BsoBI 98 CTCGGG C^[CT]CG[GA]G 311 CTCGGG BsrI 405 ACTGG ACTGG(1/-1) 722 CCAGT Cfr13I 314 GGGCC G^G[ACGT]CC 834 GGACC DdeI 282 CTCAG C^T[ACGT]AG 1027 CTCAG DpnII 772 GATC ^GATC 914 GATC HphI 7 GGTGA GGTGA(8/7) 691 TCACC MboI 772 GATC ^GATC 914 GATC MslI 253 CATCAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 334 CATAAGTATG NdeII 772 GATC ^GATC 914 GATC PleI 309 GACTC GAGTC(4/5) 619 GACTC Sau3AI 772 GATC ^GATC 914 GATC Sau96I 314 GGGCC G^G[ACGT]CC 834 GGACC TaqI 912 TCGA T^CGA 935 TCGA TfiI 481 GAATC G^A[AT]TC 799 GAATC TthHB8I 912 TCGA T^CGA 935 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AfaI AluI BfaI BseRI BsiYI BslI BsmAI BssKI BstF5I BstNI Cac8I EcoRII FokI HaeIII HinfI MaeI MboII MnlI MseI MvaI MwoI NlaIV RsaI ScrFI SfaNI Tru9I Tsp509I TspRI