################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AviII BbeI BbrPI BbvCI BclI BfrI BsaBI BsaHI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI Ksp632I KspI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp509I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AspI 580 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 725 TGGCCA TGG^CCA BamHI 547 GGATCC G^GATCC BbsI 260 GTCTTC GAAGAC(2/6) BcgI 27 GCAGAACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 80 GCCCCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 311 AGATCT A^GATCT BlpI 1140 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1140 GCTGAGC GC^T[ACGT]AGC BpuAI 260 GTCTTC GAAGAC(2/6) BsaAI 574 TACGTG [CT]AC^GT[GA] BsaI 868 GGTCTC GGTCTC(1/5) BsmI 1200 GCATTC GAATGC(1/-1) BspMI 3 GCAGGT ACCTGC(4/8) BssHII 1127 GCGCGC G^CGCGC CelII 1140 GCTGAGC GC^T[ACGT]AGC FseI 101 GGCCGGCC GGCCGG^CC HaeII 186 AGCGCC [GA]GCGC^[CT] HphI 1011 TCACC GGTGA(8/7) MaeII 575 ACGT A^CGT MluNI 725 TGGCCA TGG^CCA MscI 725 TGGCCA TGG^CCA MseI 514 TTAA T^TAA NcoI 722 CCATGG C^CATGG PflFI 580 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 657 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 896 GAGTC GAGTC(4/5) PshAI 642 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 698 CTGCAG CTGCA^G SacI 814 GAGCTC GAGCT^C SfaNI 801 GATGC GCATC(5/9) SmaI 82 CCCGGG CCC^GGG StuI 713 AGGCCT AGG^CCT TaqI 1096 TCGA T^CGA Tru9I 514 TTAA T^TAA Tth111I 580 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1096 TCGA T^CGA Van91I 657 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 608 CCAACTGTCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 82 CCCGGG C^CCGGG XmaI 82 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### Acc65I 286 GGTACC G^GTACC 851 GGTACC AccII 15 CGCG CG^CG 1128 CGCG AlwNI 658 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG 1135 CAGGTGCTG ApaI 78 GGGCCC GGGCC^C 197 GGGCCC Asp718I 286 GGTACC G^GTACC 851 GGTACC AvaI 82 CCCGGG C^[CT]CG[GA]G 158 CCCGAG BanI 286 GGTACC G^G[CT][GA]CC 851 GGTACC BciVI 571 GGATAC GTATCC(6/5) 268 GTATCC BpmI 562 CTCCAG CTGGAG(16/14) 749 CTCCAG BseRI 447 GAGGAG GAGGAG(10/8) 622 GAGGAG BsmFI 679 GGGAC GGGAC(10/14) 898 GTCCC BsoBI 82 CCCGGG C^[CT]CG[GA]G 158 CCCGAG BstUI 15 CGCG CG^CG 1128 CGCG BstXI 822 CCACAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 983 CCAAAGTCATGG BstYI 311 AGATCT [GA]^GATC[CT] 547 GGATCC DsaI 722 CCATGG C^C[GA][CT]GG 1028 CCGTGG KpnI 286 GGTACC GGTAC^C 851 GGTACC MaeIII 295 GTCAC ^GT[ACGT]AC 844 GTCAC MflI 311 AGATCT [GA]^GATC[CT] 547 GGATCC MslI 225 CACTGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 683 CATCCCGATG MvnI 15 CGCG CG^CG 1128 CGCG NaeI 102 GCCGGC GCC^GGC 106 GCCGGC NgoMIV 102 GCCGGC G^CCGGC 106 GCCGGC SmlI 430 CTCAAG C^T[CT][GA]AG 940 CTCAAG TfiI 123 GAATC G^A[AT]TC 744 GATTC Tsp45I 295 GTCAC ^GT[GC]AC 844 GTCAC XhoII 311 AGATCT [GA]^GATC[CT] 547 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaII AvrII BanII BbvI BcnI BfaI BlnI BmyI BsaJI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeI MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI SfcI StyI TseI TspRI