(Circular) (Six-base) MAPSORT of: pFN21AE5559.seq Check: 2982 from: 1 to: 5917 pFN21AE5559 5917 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1999 1999 Size: 5917 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3727 278 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3260 1937 Size: 1323 4594 Acc65I G'GTAC_C Cuts at: 2291 2856 3238 2291 Size: 565 382 4970 Fragments arranged by size: 4970 565 382 AclI AA'CG_TT Cuts at: 1563 4044 4417 1563 Size: 2481 373 3063 Fragments arranged by size: 3063 2481 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3980 5364 5632 1632 Size: 2348 1384 268 1917 Fragments arranged by size: 2348 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5720 AflIII A'CryG_T Cuts at: 1203 4816 5793 1203 Size: 3613 977 1327 Fragments arranged by size: 3613 1327 977 AgeI A'CCGG_T Cuts at: 4748 4748 Size: 5917 AhdI GACnn_n'nnGTC Cuts at: 4646 4646 Size: 5917 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5917 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5917 AlwNI CAG_nnn'CTG Cuts at: 2668 3145 3660 5232 2668 Size: 477 515 1572 3353 Fragments arranged by size: 3353 1572 515 477 ApaI G_GGCC'C Cuts at: 2087 2206 2087 Size: 119 5798 ApaLI G'TGCA_C Cuts at: 3974 5130 3974 Size: 1156 4761 ApoI r'AATT_y Cuts at: 1493 3226 3452 3604 5713 1493 Size: 1733 226 152 2109 1697 Fragments arranged by size: 2109 1733 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4471 160 Size: 872 3439 1606 Fragments arranged by size: 3439 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 AvaI C'yCGr_G Cuts at: 1870 1943 2087 2163 3242 1870 Size: 73 144 76 1079 4545 Fragments arranged by size: 4545 1079 144 76 73 AvrII C'CTAG_G Cuts at: 2251 2287 2422 2251 Size: 36 135 5746 Fragments arranged by size: 5746 135 36 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2283 2316 2283 Size: 33 5884 BamHI G'GATC_C Cuts at: 2552 3247 3614 2552 Size: 695 367 4855 Fragments arranged by size: 4855 695 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2291 2856 3238 4693 618 Size: 325 217 627 504 565 382 1455 1842 Fragments arranged by size: 1842 1455 627 565 504 382 325 217 BanII G_rGCy'C Cuts at: 1391 2087 2206 2823 3189 3236 1391 Size: 696 119 617 366 47 4072 Fragments arranged by size: 4072 696 617 366 119 47 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2258 928 Size: 971 359 4587 Fragments arranged by size: 4587 971 359 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2055 4108 4142 1903 Size: 34 50 34 34 2053 34 3678 Fragments arranged by size: 3678 2053 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2284 2570 3809 5019 2284 Size: 286 1239 1210 3182 Fragments arranged by size: 3182 1239 1210 286 BclI T'GATC_A Cuts at: 1548 1548 Size: 5917 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2091 4528 136 Size: 107 122 71 631 605 419 2437 1525 Fragments arranged by size: 2437 1525 631 605 419 122 107 71 BglII A'GATC_T Cuts at: 2316 5912 2316 Size: 3596 2321 BlpI GC'TnA_GC Cuts at: 3146 3334 3146 Size: 188 5729 Bme1580I G_kGCm'C Cuts at: 1790 2087 2124 2206 3978 5134 1790 Size: 297 37 82 1772 1156 2573 Fragments arranged by size: 2573 1772 1156 297 82 37 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5917 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2689 3648 4606 Size: 416 53 83 399 24 1272 959 958 Cuts at: 4606 442 Size: 1753 Fragments arranged by size: 1753 1272 959 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5917 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2740 2740 Size: 5917 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2552 2739 4577 1239 Size: 1313 187 1838 2579 Fragments arranged by size: 2579 1838 1313 187 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2420 2930 4042 4907 5205 2420 Size: 510 1112 865 298 3132 Fragments arranged by size: 3132 1112 865 510 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2879 4580 882 Size: 617 1380 1701 2219 Fragments arranged by size: 2219 1701 1380 617 BsaAI yAC'GTr Cuts at: 493 1204 2581 3708 493 Size: 711 1377 1127 2702 Fragments arranged by size: 2702 1377 1127 711 BsaBI GATnn'nnATC Cuts at: 5911 5911 Size: 5917 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3724 4106 4741 275 Size: 53 83 186 936 2191 382 635 1451 Fragments arranged by size: 2191 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4350 4748 5022 5169 5580 4350 Size: 398 274 147 411 4687 Fragments arranged by size: 4687 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5692 Fragments arranged by size: 5692 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2467 2642 1186 Size: 1281 175 4461 Fragments arranged by size: 4461 1281 175 BseYI C'CCAG_C Cuts at: 2188 2198 2572 3022 3039 3091 5120 5616 2188 Size: 10 374 450 17 52 2029 496 2489 Fragments arranged by size: 2489 2029 496 450 374 52 17 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2154 2978 3013 1163 Size: 573 418 824 35 4067 Fragments arranged by size: 4067 824 573 418 35 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2081 2112 2224 3367 4128 5156 Size: 275 186 80 31 112 1143 761 1028 Cuts at: 5156 1540 Size: 2301 Fragments arranged by size: 2301 1143 1028 761 275 186 112 80 31 BsiHKAI G_wGCw'C Cuts at: 2219 2778 2823 3978 4063 5134 2219 Size: 559 45 1155 85 1071 3002 Fragments arranged by size: 3002 1155 1071 559 85 45 BsmI GAATG_Cn' Cuts at: 3205 3428 3521 3205 Size: 223 93 5601 Fragments arranged by size: 5601 223 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2124 2206 2219 2778 2823 3189 Size: 399 297 37 82 13 559 45 366 Cuts at: 3189 3236 3978 4063 5134 1391 Size: 47 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 559 399 366 297 85 82 47 45 37 13 BspEI T'CCGG_A Cuts at: 5580 5580 Size: 5917 BspHI T'CATG_A Cuts at: 3806 5536 3806 Size: 1730 4187 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 1999 3272 844 Size: 1155 1273 3489 Fragments arranged by size: 3489 1273 1155 BsrBI CCG'CTC Cuts at: 1394 1823 3804 1394 Size: 429 1981 3507 Fragments arranged by size: 3507 1981 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4412 4586 65 Size: 1183 3164 174 1396 Fragments arranged by size: 3164 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2107 2111 2219 4561 4748 1700 Size: 110 297 4 108 2342 187 2869 Fragments arranged by size: 2869 2342 297 187 110 108 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5917 BssHII G'CGCG_C Cuts at: 1925 3132 1925 Size: 1207 4710 BssSI C'ACGA_G Cuts at: 3977 4989 3977 Size: 1012 4905 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5917 BstBI TT'CG_AA Cuts at: 4721 4721 Size: 5917 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5917 BstXI CCAn_nnnn'nTGG Cuts at: 2834 2995 2834 Size: 161 5756 BstYI r'GATC_y Cuts at: 1972 2316 2552 3247 3614 3999 4016 5468 5554 Size: 344 236 695 367 385 17 1452 86 Cuts at: 5554 5566 5912 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 695 385 367 346 344 236 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2727 3033 513 Size: 552 1662 306 3397 Fragments arranged by size: 3397 1662 552 306 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5178 Fragments arranged by size: 5178 683 56 BtsI GCAGTG_nn' Cuts at: 2059 3073 3433 4227 4247 2059 Size: 1014 360 794 20 3729 Fragments arranged by size: 3729 1014 794 360 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5917 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5882 DraI TTT'AAA Cuts at: 3221 3221 Size: 5917 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4924 809 Size: 507 3608 1802 Fragments arranged by size: 3608 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2078 2109 2221 Size: 54 1425 50 275 18 248 31 112 Cuts at: 2221 2730 3364 4253 5882 8 Size: 509 634 889 1629 43 Fragments arranged by size: 1629 1425 889 634 509 275 248 112 54 50 43 31 18 EagI C'GGCC_G Cuts at: 1537 1812 2078 2109 2221 3364 1537 Size: 275 266 31 112 1143 4090 Fragments arranged by size: 4090 1143 275 266 112 31 EarI CTCTTCn'nnn_ Cuts at: 1353 3847 1353 Size: 2494 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4504 4878 5024 387 Size: 1064 138 2915 374 146 1280 Fragments arranged by size: 2915 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2821 2821 Size: 5917 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2552 2739 3980 4577 5364 5632 1239 Size: 393 920 187 1241 597 787 268 1524 Fragments arranged by size: 1524 1241 920 787 597 393 268 187 EcoO109I rG'GnC_Cy Cuts at: 2084 2202 2203 2665 2919 2084 Size: 118 1 462 254 5082 Fragments arranged by size: 5082 462 254 118 1 EcoRI G'AATT_C Cuts at: 3226 3604 3226 Size: 378 5539 FseI GG_CCGG'CC Cuts at: 2111 2111 Size: 5917 FspI TGC'GCA Cuts at: 4423 4423 Size: 5917 HaeII r_GCGC'y Cuts at: 1678 2195 5064 1678 Size: 517 2869 2531 Fragments arranged by size: 2869 2531 517 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4558 4590 4632 4664 1373 Size: 32 135 32 2986 32 42 32 2626 Fragments arranged by size: 2986 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3261 3507 669 Size: 988 281 1323 246 3079 Fragments arranged by size: 3079 1323 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5917 HpaI GTT'AAC Cuts at: 3507 3507 Size: 5917 KpnI G_GTAC'C Cuts at: 2295 2860 3242 2295 Size: 565 382 4970 Fragments arranged by size: 4970 565 382 MfeI C'AATT_G Cuts at: 3516 3516 Size: 5917 MluI A'CGCG_T Cuts at: 5793 5793 Size: 5917 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2429 2821 5031 5215 1305 Size: 1124 392 2210 184 2007 Fragments arranged by size: 2210 2007 1124 392 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2732 10 Size: 54 1425 343 900 3195 Fragments arranged by size: 3195 1425 900 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2234 2692 3875 4234 4393 518 Size: 648 843 225 458 1183 359 159 2042 Fragments arranged by size: 2042 1183 843 648 458 359 225 159 MspA1I CmG'CkG Cuts at: 1606 2198 2572 2812 3022 3154 3333 4010 5158 Size: 592 374 240 210 132 179 677 1148 Cuts at: 5158 5403 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 592 374 245 240 210 179 132 NaeI GCC'GGC Cuts at: 2109 2113 2109 Size: 4 5913 NcoI C'CATG_G Cuts at: 513 1065 2727 513 Size: 552 1662 3703 Fragments arranged by size: 3703 1662 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5917 NgoMIV G'CCGG_C Cuts at: 2107 2111 2107 Size: 4 5913 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5917 NotI GC'GGCC_GC Cuts at: 3364 3364 Size: 5917 NspI r_CATG'y Cuts at: 1132 1229 3275 4820 1132 Size: 97 2046 1545 2229 Fragments arranged by size: 2229 2046 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5917 PciI A'CATG_T Cuts at: 4816 4816 Size: 5917 PflMI CCAn_nnn'nTGG Cuts at: 1108 2668 1108 Size: 1560 4357 PmeI GTTT'AAAC Cuts at: 3221 3221 Size: 5917 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3986 4018 5499 5531 3986 Size: 32 1481 32 4372 Fragments arranged by size: 4372 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2651 1613 Size: 1038 4879 PsiI TTA'TAA Cuts at: 3487 3487 Size: 5917 PspOMI G'GGCC_C Cuts at: 2083 2202 2083 Size: 119 5798 PstI C_TGCA'G Cuts at: 830 2707 3269 830 Size: 1877 562 3478 Fragments arranged by size: 3478 1877 562 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5917 PvuII CAG'CTG Cuts at: 2572 2812 3022 3154 2572 Size: 240 210 132 5335 Fragments arranged by size: 5335 240 210 132 SacI G_AGCT'C Cuts at: 2823 2823 Size: 5917 SalI G'TCGA_C Cuts at: 1936 3259 1936 Size: 1323 4594 SbfI CC_TGCA'GG Cuts at: 3269 3269 Size: 5917 ScaI AGT'ACT Cuts at: 4165 5877 4165 Size: 1712 4205 SfcI C'TryA_G Cuts at: 826 1045 2349 2703 3137 3265 4400 5081 5272 Size: 219 1304 354 434 128 1135 681 191 Cuts at: 5272 826 Size: 1471 Fragments arranged by size: 1471 1304 1135 681 434 354 219 191 128 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5917 SmaI CCC'GGG Cuts at: 1872 2089 3244 1872 Size: 217 1155 4545 Fragments arranged by size: 4545 1155 217 SmlI C'TyrA_G Cuts at: 820 1017 1943 2435 2945 4021 4922 5184 820 Size: 197 926 492 510 1076 901 262 1553 Fragments arranged by size: 1553 1076 926 901 510 492 262 197 SnaBI TAC'GTA Cuts at: 493 3708 493 Size: 3215 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5917 SphI G_CATG'C Cuts at: 1132 3275 1132 Size: 2143 3774 SspI AAT'ATT Cuts at: 5 52 3841 5 Size: 47 3789 2081 Fragments arranged by size: 3789 2081 47 StuI AGG'CCT Cuts at: 1517 2720 1517 Size: 1203 4714 StyI C'CwwG_G Cuts at: 513 1065 2157 2251 2287 2422 2727 3356 513 Size: 552 1092 94 36 135 305 629 3074 Fragments arranged by size: 3074 1092 629 552 305 135 94 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3944 3961 4114 4299 4744 325 Size: 1255 2364 17 153 185 445 1498 Fragments arranged by size: 2364 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4163 5875 96 Size: 274 80 33 51 1443 2186 1712 138 Fragments arranged by size: 2186 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2051 2083 5877 5909 2051 Size: 32 3794 32 2059 Fragments arranged by size: 3794 2059 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2588 1316 Size: 1272 4645 XbaI T'CTAG_A Cuts at: 3253 3253 Size: 5917 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2620 2620 Size: 5917 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5917 XmaI C'CCGG_G Cuts at: 1870 2087 3242 1870 Size: 217 1155 4545 Fragments arranged by size: 4545 1155 217 XmnI GAAnn'nnTTC Cuts at: 4046 4046 Size: 5917 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3725 276 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI