(Linear) (Six-base) MAPSORT of: KIEE5559.seq Check: 5036 from: 1 to: 1212 KIEE5559.orf With 182 enzymes: SgfI * January 29, 2014 12:05 .. Acc65I G'GTAC_C Cuts at: 0 286 851 1212 Size: 286 565 361 Fragments arranged by size: 565 361 286 AlwNI CAG_nnn'CTG Cuts at: 0 663 1140 1212 Size: 663 477 72 Fragments arranged by size: 663 477 72 ApaI G_GGCC'C Cuts at: 0 82 201 1212 Size: 82 119 1011 Fragments arranged by size: 1011 119 82 AvaI C'yCGr_G Cuts at: 0 82 158 1212 Size: 82 76 1054 Fragments arranged by size: 1054 82 76 AvrII C'CTAG_G Cuts at: 0 246 282 417 1212 Size: 246 36 135 795 Fragments arranged by size: 795 246 135 36 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 278 311 1212 Size: 278 33 901 Fragments arranged by size: 901 278 33 BamHI G'GATC_C Cuts at: 0 547 1212 Size: 547 665 BanI G'GyrC_C Cuts at: 0 286 851 1212 Size: 286 565 361 Fragments arranged by size: 565 361 286 BanII G_rGCy'C Cuts at: 0 82 201 818 1184 1212 Size: 82 119 617 366 28 Fragments arranged by size: 617 366 119 82 28 BbsI GAAGACnn'nnnn_ Cuts at: 0 253 1212 Size: 253 959 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 16 50 1212 Size: 16 34 1162 Fragments arranged by size: 1162 34 16 BciVI GTATCCnnnnn_n' Cuts at: 0 279 565 1212 Size: 279 286 647 Fragments arranged by size: 647 286 279 BglI GCCn_nnn'nGGC Cuts at: 0 86 1212 Size: 86 1126 BglII A'GATC_T Cuts at: 0 311 1212 Size: 311 901 BlpI GC'TnA_GC Cuts at: 0 1141 1212 Size: 1141 71 Bme1580I G_kGCm'C Cuts at: 0 82 119 201 1212 Size: 82 37 82 1011 Fragments arranged by size: 1011 82 82 37 BmrI ACTGGGnnnn_n' Cuts at: 0 684 1212 Size: 684 528 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 735 1212 Size: 735 477 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 547 734 1212 Size: 547 187 478 Fragments arranged by size: 547 478 187 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 415 925 1212 Size: 415 510 287 Fragments arranged by size: 510 415 287 BsaI GGTCTCn'nnnn_ Cuts at: 0 874 1212 Size: 874 338 BsaAI yAC'GTr Cuts at: 0 576 1212 Size: 576 636 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 462 637 1212 Size: 462 175 575 Fragments arranged by size: 575 462 175 BseYI C'CCAG_C Cuts at: 0 183 193 567 1017 1034 1086 1212 Size: 183 10 374 450 17 52 126 Fragments arranged by size: 450 374 183 126 52 17 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 149 973 1008 1212 Size: 149 824 35 204 Fragments arranged by size: 824 204 149 35 BsiEI CG_ry'CG Cuts at: 0 76 107 219 1212 Size: 76 31 112 993 Fragments arranged by size: 993 112 76 31 BsiHKAI G_wGCw'C Cuts at: 0 214 773 818 1212 Size: 214 559 45 394 Fragments arranged by size: 559 394 214 45 BsmI GAATG_Cn' Cuts at: 0 1200 1212 Size: 1200 12 Bsp1286I G_dGCh'C Cuts at: 0 82 119 201 214 773 818 1184 1212 Size: 82 37 82 13 559 45 366 28 Fragments arranged by size: 559 366 82 82 45 37 28 13 BsrFI r'CCGG_y Cuts at: 0 102 106 214 1212 Size: 102 4 108 998 Fragments arranged by size: 998 108 102 4 BssHII G'CGCG_C Cuts at: 0 1127 1212 Size: 1127 85 BstXI CCAn_nnnn'nTGG Cuts at: 0 829 990 1212 Size: 829 161 222 Fragments arranged by size: 829 222 161 BstYI r'GATC_y Cuts at: 0 311 547 1212 Size: 311 236 665 Fragments arranged by size: 665 311 236 BtgI C'CryG_G Cuts at: 0 722 1028 1212 Size: 722 306 184 Fragments arranged by size: 722 306 184 BtsI GCAGTG_nn' Cuts at: 0 54 1068 1212 Size: 54 1014 144 Fragments arranged by size: 1014 144 54 EaeI y'GGCC_r Cuts at: 0 73 104 216 725 1212 Size: 73 31 112 509 487 Fragments arranged by size: 509 487 112 73 31 EagI C'GGCC_G Cuts at: 0 73 104 216 1212 Size: 73 31 112 996 Fragments arranged by size: 996 112 73 31 EcoICRI GAG'CTC Cuts at: 0 816 1212 Size: 816 396 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 547 734 1212 Size: 547 187 478 Fragments arranged by size: 547 478 187 EcoO109I rG'GnC_Cy Cuts at: 0 79 197 198 660 914 1212 Size: 79 118 1 462 254 298 Fragments arranged by size: 462 298 254 118 79 1 FseI GG_CCGG'CC Cuts at: 0 106 1212 Size: 106 1106 HaeII r_GCGC'y Cuts at: 0 190 1212 Size: 190 1022 KpnI G_GTAC'C Cuts at: 0 290 855 1212 Size: 290 565 357 Fragments arranged by size: 565 357 290 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 424 816 1212 Size: 424 392 396 Fragments arranged by size: 424 396 392 MscI TGG'CCA Cuts at: 0 727 1212 Size: 727 485 MslI CAynn'nnrTG Cuts at: 0 229 687 1212 Size: 229 458 525 Fragments arranged by size: 525 458 229 MspA1I CmG'CkG Cuts at: 0 193 567 807 1017 1149 1212 Size: 193 374 240 210 132 63 Fragments arranged by size: 374 240 210 193 132 63 NaeI GCC'GGC Cuts at: 0 104 108 1212 Size: 104 4 1104 Fragments arranged by size: 1104 104 4 NcoI C'CATG_G Cuts at: 0 722 1212 Size: 722 490 NgoMIV G'CCGG_C Cuts at: 0 102 106 1212 Size: 102 4 1106 Fragments arranged by size: 1106 102 4 PflMI CCAn_nnn'nTGG Cuts at: 0 663 1212 Size: 663 549 PshAI GACnn'nnGTC Cuts at: 0 646 1212 Size: 646 566 PspOMI G'GGCC_C Cuts at: 0 78 197 1212 Size: 78 119 1015 Fragments arranged by size: 1015 119 78 PstI C_TGCA'G Cuts at: 0 702 1212 Size: 702 510 PvuII CAG'CTG Cuts at: 0 567 807 1017 1149 1212 Size: 567 240 210 132 63 Fragments arranged by size: 567 240 210 132 63 SacI G_AGCT'C Cuts at: 0 818 1212 Size: 818 394 SfcI C'TryA_G Cuts at: 0 344 698 1132 1212 Size: 344 354 434 80 Fragments arranged by size: 434 354 344 80 SmaI CCC'GGG Cuts at: 0 84 1212 Size: 84 1128 SmlI C'TyrA_G Cuts at: 0 430 940 1212 Size: 430 510 272 Fragments arranged by size: 510 430 272 StuI AGG'CCT Cuts at: 0 715 1212 Size: 715 497 StyI C'CwwG_G Cuts at: 0 152 246 282 417 722 1212 Size: 152 94 36 135 305 490 Fragments arranged by size: 490 305 152 135 94 36 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 46 78 1212 Size: 46 32 1134 Fragments arranged by size: 1134 46 32 Tth111I GACn'n_nGTC Cuts at: 0 583 1212 Size: 583 629 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 615 1212 Size: 615 597 XmaI C'CCGG_G Cuts at: 0 82 1212 Size: 82 1130 Enzymes that do cut: Acc65I AlwNI ApaI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrFI BssHII BstXI BstYI BtgI BtsI EaeI EagI EcoICRI Eco57MI EcoO109I FseI HaeII KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV PflMI PshAI PspOMI PstI PvuII SacI SfcI SmaI SmlI StuI StyI TstI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII AccI AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI BbeI BbvCI BclI BfrBI BmgBI BmtI Bpu10I BsaBI BsaHI BsaWI BsaXI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EarI EciI EcoNI EcoRI EcoRV FalI FspI FspAI Hin4I HincII HindIII HpaI KasI MfeI MluI NarI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI XbaI XhoI XmnI ZraI