(Circular) (Six-base) MAPSORT of: pFN21AE3754.seq Check: 2664 from: 1 to: 5452 pFN21AE3754 5452 bp With 182 enzymes: SgfI * March 28, 2012 12:06 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2366 2366 Size: 5452 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3262 278 Size: 53 83 186 936 1726 2468 Fragments arranged by size: 2468 1726 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2529 2795 1937 Size: 592 266 4594 Fragments arranged by size: 4594 592 266 Acc65I G'GTAC_C Cuts at: 2773 2773 Size: 5452 AclI AA'CG_TT Cuts at: 1563 3579 3952 1563 Size: 2016 373 3063 Fragments arranged by size: 3063 2016 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2555 3515 4899 5167 1632 Size: 923 960 1384 268 1917 Fragments arranged by size: 1917 1384 960 923 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5255 AflIII A'CryG_T Cuts at: 1203 4351 5328 1203 Size: 3148 977 1327 Fragments arranged by size: 3148 1327 977 AgeI A'CCGG_T Cuts at: 4283 4283 Size: 5452 AhdI GACnn_n'nnGTC Cuts at: 4181 4181 Size: 5452 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5452 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2078 1184 Size: 894 4558 AlwNI CAG_nnn'CTG Cuts at: 2597 3195 4767 2597 Size: 598 1572 3282 Fragments arranged by size: 3282 1572 598 ApaI G_GGCC'C Cuts at: 2619 2701 2619 Size: 82 5370 ApaLI G'TGCA_C Cuts at: 2286 2504 3509 4665 2286 Size: 218 1005 1156 3073 Fragments arranged by size: 3073 1156 1005 218 ApoI r'AATT_y Cuts at: 1493 2585 2761 2987 3139 5248 1493 Size: 1092 176 226 152 2109 1697 Fragments arranged by size: 2109 1697 1092 226 176 152 AseI AT'TA_AT Cuts at: 160 1032 4006 160 Size: 872 2974 1606 Fragments arranged by size: 2974 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5452 AvaI C'yCGr_G Cuts at: 1870 1943 2472 2612 2777 1870 Size: 73 529 140 165 4545 Fragments arranged by size: 4545 529 165 140 73 BamHI G'GATC_C Cuts at: 2242 2679 2782 3149 2242 Size: 437 103 367 4545 Fragments arranged by size: 4545 437 367 103 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2037 2054 2773 4228 618 Size: 325 217 627 250 17 719 1455 1842 Fragments arranged by size: 1842 1455 719 627 325 250 217 17 BanII G_rGCy'C Cuts at: 1391 2314 2484 2619 2701 2771 1391 Size: 923 170 135 82 70 4072 Fragments arranged by size: 4072 923 170 135 82 70 BbeI G_GCGC'C Cuts at: 2041 2041 Size: 5452 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4481 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3643 3677 1903 Size: 34 1706 34 3678 Fragments arranged by size: 3678 1706 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3344 4554 3344 Size: 1210 4242 BclI T'GATC_A Cuts at: 1548 1548 Size: 5452 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2600 4063 136 Size: 107 122 71 631 605 928 1463 1525 Fragments arranged by size: 1525 1463 928 631 605 122 107 71 BglII A'GATC_T Cuts at: 5447 5447 Size: 5452 BlpI GC'TnA_GC Cuts at: 2869 2869 Size: 5452 Bme1580I G_kGCm'C Cuts at: 1790 2057 2290 2508 2619 2701 3513 4669 1790 Size: 267 233 218 111 82 812 1156 2573 Fragments arranged by size: 2573 1156 812 267 233 218 111 82 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5452 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3183 4141 442 Size: 416 53 83 399 24 1766 958 1753 Fragments arranged by size: 1766 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5452 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2605 2605 Size: 5452 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2410 2600 4112 1239 Size: 1171 190 1512 2579 Fragments arranged by size: 2579 1512 1171 190 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2690 3577 4442 4740 2690 Size: 887 865 298 3402 Fragments arranged by size: 3402 887 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4115 882 Size: 617 2616 2219 Fragments arranged by size: 2616 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3243 493 Size: 711 2039 2702 Fragments arranged by size: 2702 2039 711 BsaBI GATnn'nnATC Cuts at: 5446 5446 Size: 5452 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2038 3259 3641 4276 Size: 53 83 186 936 505 1221 382 635 Cuts at: 4276 275 Size: 1451 Fragments arranged by size: 1451 1221 936 635 505 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3885 4283 4557 4704 5115 3885 Size: 398 274 147 411 4222 Fragments arranged by size: 4222 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5227 Fragments arranged by size: 5227 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2192 2207 1186 Size: 1006 15 4431 Fragments arranged by size: 4431 1006 15 BseYI C'CCAG_C Cuts at: 2080 2400 2477 2619 4655 5151 2080 Size: 320 77 142 2036 496 2381 Fragments arranged by size: 2381 2036 496 320 142 77 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4879 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2902 3663 4691 1540 Size: 275 186 901 761 1028 2301 Fragments arranged by size: 2301 1028 901 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2290 2508 3513 3598 4669 2290 Size: 218 1005 85 1071 3073 Fragments arranged by size: 3073 1071 1005 218 85 BsmI GAATG_Cn' Cuts at: 2963 3056 2963 Size: 93 5359 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2290 2314 2484 2508 2619 2701 Size: 399 267 233 24 170 24 111 82 Cuts at: 2701 2771 3513 3598 4669 1391 Size: 70 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 267 233 170 111 85 82 70 24 24 BspEI T'CCGG_A Cuts at: 5115 5115 Size: 5452 BspHI T'CATG_A Cuts at: 3341 5071 3341 Size: 1730 3722 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2366 2807 844 Size: 1522 441 3489 Fragments arranged by size: 3489 1522 441 BsrBI CCG'CTC Cuts at: 1394 1823 2074 3339 1394 Size: 429 251 1265 3507 Fragments arranged by size: 3507 1265 429 251 BsrDI GCAATG_nn' Cuts at: 65 1248 3947 4121 65 Size: 1183 2699 174 1396 Fragments arranged by size: 2699 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4096 4283 1700 Size: 110 2286 187 2869 Fragments arranged by size: 2869 2286 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5452 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5452 BssSI C'ACGA_G Cuts at: 3512 4524 3512 Size: 1012 4440 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5452 BstBI TT'CG_AA Cuts at: 4256 4256 Size: 5452 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5452 BstXI CCAn_nnnn'nTGG Cuts at: 2642 2642 Size: 5452 BstYI r'GATC_y Cuts at: 1972 2242 2679 2782 3149 3534 3551 5003 5089 Size: 270 437 103 367 385 17 1452 86 Cuts at: 5089 5101 5447 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 437 385 367 346 270 103 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2124 513 Size: 552 939 120 3841 Fragments arranged by size: 3841 939 552 120 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2133 487 Size: 683 56 907 3806 Fragments arranged by size: 3806 907 683 56 BtsI GCAGTG_nn' Cuts at: 2595 2968 3762 3782 2595 Size: 373 794 20 4265 Fragments arranged by size: 4265 794 373 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5452 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5417 DraI TTT'AAA Cuts at: 2756 2756 Size: 5452 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4459 809 Size: 507 3143 1802 Fragments arranged by size: 3143 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2121 2660 Size: 54 1425 50 275 18 177 114 539 Cuts at: 2660 2899 3788 5417 8 Size: 239 889 1629 43 Fragments arranged by size: 1629 1425 889 539 275 239 177 114 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2899 1537 Size: 275 1087 4090 Fragments arranged by size: 4090 1087 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2311 2744 3382 1353 Size: 958 433 638 3423 Fragments arranged by size: 3423 958 638 433 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2044 2146 2615 4039 4413 4559 Size: 1064 138 455 102 469 1424 374 146 Cuts at: 4559 387 Size: 1280 Fragments arranged by size: 1424 1280 1064 469 455 374 146 138 102 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2410 2555 2600 3515 4112 4899 5167 Size: 393 778 145 45 915 597 787 268 Cuts at: 5167 1239 Size: 1524 Fragments arranged by size: 1524 915 787 778 597 393 268 145 45 EcoNI CCTnn'n_nnAGG Cuts at: 2633 2633 Size: 5452 EcoO109I rG'GnC_Cy Cuts at: 2203 2616 2667 2203 Size: 413 51 4988 Fragments arranged by size: 4988 413 51 EcoRI G'AATT_C Cuts at: 2585 2761 3139 2585 Size: 176 378 4898 Fragments arranged by size: 4898 378 176 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2560 2560 Size: 5452 FspI TGC'GCA Cuts at: 3958 3958 Size: 5452 HaeII r_GCGC'y Cuts at: 1678 2041 2099 4599 1678 Size: 363 58 2500 2531 Fragments arranged by size: 2531 2500 363 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4093 4125 4167 4199 1373 Size: 32 135 32 2521 32 42 32 2626 Fragments arranged by size: 2626 2521 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2796 3042 669 Size: 988 281 858 246 3079 Fragments arranged by size: 3079 988 858 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5452 HpaI GTT'AAC Cuts at: 3042 3042 Size: 5452 KasI G'GCGC_C Cuts at: 2037 2037 Size: 5452 KpnI G_GTAC'C Cuts at: 2777 2777 Size: 5452 MfeI C'AATT_G Cuts at: 3051 3051 Size: 5452 MluI A'CGCG_T Cuts at: 5328 5328 Size: 5452 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1997 2190 4566 4750 1305 Size: 692 193 2376 184 2007 Fragments arranged by size: 2376 2007 692 193 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3630 Fragments arranged by size: 3630 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2511 3410 3769 3928 518 Size: 648 1345 899 359 159 2042 Fragments arranged by size: 2042 1345 899 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2868 3545 4693 4938 1606 Size: 1262 677 1148 245 2120 Fragments arranged by size: 2120 1262 1148 677 245 NarI GG'CG_CC Cuts at: 2038 2038 Size: 5452 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3961 Fragments arranged by size: 3961 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5452 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5452 NotI GC'GGCC_GC Cuts at: 2899 2899 Size: 5452 NspI r_CATG'y Cuts at: 1132 1229 2810 4355 1132 Size: 97 1581 1545 2229 Fragments arranged by size: 2229 1581 1545 97 PasI CC'CwG_GG Cuts at: 1348 2694 1348 Size: 1346 4106 PciI A'CATG_T Cuts at: 4351 4351 Size: 5452 PflMI CCAn_nnn'nTGG Cuts at: 1108 2354 1108 Size: 1246 4206 PmeI GTTT'AAAC Cuts at: 2756 2756 Size: 5452 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3521 3553 5034 5066 3521 Size: 32 1481 32 3907 Fragments arranged by size: 3907 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2203 2667 2203 Size: 464 4988 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5452 PsiI TTA'TAA Cuts at: 3022 3022 Size: 5452 PspOMI G'GGCC_C Cuts at: 2615 2697 2615 Size: 82 5370 PstI C_TGCA'G Cuts at: 830 2804 830 Size: 1974 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5452 SalI G'TCGA_C Cuts at: 1936 2794 1936 Size: 858 4594 SapI GCTCTTCn'nnn_ Cuts at: 2311 2311 Size: 5452 SbfI CC_TGCA'GG Cuts at: 2804 2804 Size: 5452 ScaI AGT'ACT Cuts at: 3700 5412 3700 Size: 1712 3740 SfcI C'TryA_G Cuts at: 826 1045 2800 3935 4616 4807 826 Size: 219 1755 1135 681 191 1471 Fragments arranged by size: 1755 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2039 2039 Size: 5452 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5452 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5452 SmaI CCC'GGG Cuts at: 1872 2474 2779 1872 Size: 602 305 4545 Fragments arranged by size: 4545 602 305 SmlI C'TyrA_G Cuts at: 820 1017 1943 2705 3556 4457 4719 820 Size: 197 926 762 851 901 262 1553 Fragments arranged by size: 1553 926 901 851 762 262 197 SnaBI TAC'GTA Cuts at: 493 3243 493 Size: 2750 2702 SpeI A'CTAG_T Cuts at: 152 2266 152 Size: 2114 3338 SphI G_CATG'C Cuts at: 1132 2810 1132 Size: 1678 3774 SspI AAT'ATT Cuts at: 5 52 3376 5 Size: 47 3324 2081 Fragments arranged by size: 3324 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5452 StyI C'CwwG_G Cuts at: 513 1065 2004 2891 513 Size: 552 939 887 3074 Fragments arranged by size: 3074 939 887 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3479 3496 3649 3834 4279 325 Size: 1255 1899 17 153 185 445 1498 Fragments arranged by size: 1899 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2493 3698 5410 Size: 274 80 33 51 1443 516 1205 1712 Cuts at: 5410 96 Size: 138 Fragments arranged by size: 1712 1443 1205 516 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2380 2412 5412 5444 2380 Size: 32 3000 32 2388 Fragments arranged by size: 3000 2388 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5452 XbaI T'CTAG_A Cuts at: 2788 2788 Size: 5452 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5452 XmaI C'CCGG_G Cuts at: 1870 2472 2777 1870 Size: 602 305 4545 Fragments arranged by size: 4545 602 305 XmnI GAAnn'nnTTC Cuts at: 3581 3581 Size: 5452 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3260 276 Size: 53 83 186 936 1726 2468 Fragments arranged by size: 2468 1726 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI