(Circular) (Six-base) MAPSORT of: pFN21AE2468.seq Check: 5625 from: 1 to: 6409 pFN21AE2468 6409 bp With 182 enzymes: SgfI * December 8, 2014 13:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2224 2224 Size: 6409 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4219 278 Size: 53 83 186 936 2683 2468 Fragments arranged by size: 2683 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3752 1937 Size: 1815 4594 Acc65I G'GTAC_C Cuts at: 3730 3730 Size: 6409 AclI AA'CG_TT Cuts at: 1563 4536 4909 1563 Size: 2973 373 3063 Fragments arranged by size: 3063 2973 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4472 5856 6124 1632 Size: 2840 1384 268 1917 Fragments arranged by size: 2840 1917 1384 268 AfeI AGC'GCT Cuts at: 2309 2309 Size: 6409 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6212 AflIII A'CryG_T Cuts at: 1203 5308 6285 1203 Size: 4105 977 1327 Fragments arranged by size: 4105 1327 977 AgeI A'CCGG_T Cuts at: 5240 5240 Size: 6409 AhdI GACnn_n'nnGTC Cuts at: 5138 5138 Size: 6409 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6409 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3254 1184 Size: 2070 4339 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2741 2773 2741 Size: 32 6377 AlwNI CAG_nnn'CTG Cuts at: 2221 4152 5724 2221 Size: 1931 1572 2906 Fragments arranged by size: 2906 1931 1572 ApaI G_GGCC'C Cuts at: 2937 2937 Size: 6409 ApaLI G'TGCA_C Cuts at: 2152 3388 4466 5622 2152 Size: 1236 1078 1156 2939 Fragments arranged by size: 2939 1236 1156 1078 ApoI r'AATT_y Cuts at: 1493 3718 3944 4096 6205 1493 Size: 2225 226 152 2109 1697 Fragments arranged by size: 2225 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4963 160 Size: 872 3931 1606 Fragments arranged by size: 3931 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6409 AvaI C'yCGr_G Cuts at: 1870 1943 3000 3283 3734 1870 Size: 73 1057 283 451 4545 Fragments arranged by size: 4545 1057 451 283 73 BamHI G'GATC_C Cuts at: 3272 3739 4106 3272 Size: 467 367 5575 Fragments arranged by size: 5575 467 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2449 2663 3558 3730 5185 Size: 325 217 627 662 214 895 172 1455 Cuts at: 5185 618 Size: 1842 Fragments arranged by size: 1842 1455 895 662 627 325 217 214 172 BanII G_rGCy'C Cuts at: 1391 2937 3321 3728 1391 Size: 1546 384 407 4072 Fragments arranged by size: 4072 1546 407 384 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2601 3607 928 Size: 971 702 1006 3730 Fragments arranged by size: 3730 1006 971 702 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2725 2759 2876 2910 4600 4634 1903 Size: 34 788 34 117 34 1690 34 3678 Fragments arranged by size: 3678 1690 788 117 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4301 5511 4301 Size: 1210 5199 BclI T'GATC_A Cuts at: 1548 1548 Size: 6409 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2371 2504 3025 Size: 107 122 71 631 605 699 133 521 Cuts at: 3025 3080 5020 136 Size: 55 1940 1525 Fragments arranged by size: 1940 1525 699 631 605 521 133 122 107 71 55 BglII A'GATC_T Cuts at: 6404 6404 Size: 6409 BlpI GC'TnA_GC Cuts at: 2018 3826 2018 Size: 1808 4601 Bme1580I G_kGCm'C Cuts at: 1790 2156 2454 2666 2937 3225 3336 3392 3561 Size: 366 298 212 271 288 111 56 169 Cuts at: 3561 4470 5626 1790 Size: 909 1156 2573 Fragments arranged by size: 2573 1156 909 366 298 288 271 212 169 111 56 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6409 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3691 4140 5098 Size: 416 53 83 399 24 2274 449 958 Cuts at: 5098 442 Size: 1753 Fragments arranged by size: 2274 1753 958 449 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6409 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2212 2534 2804 5069 1239 Size: 973 322 270 2265 2579 Fragments arranged by size: 2579 2265 973 322 270 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2885 3548 4534 5399 5697 2885 Size: 663 986 865 298 3597 Fragments arranged by size: 3597 986 865 663 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2997 5072 882 Size: 617 1498 2075 2219 Fragments arranged by size: 2219 2075 1498 617 BsaAI yAC'GTr Cuts at: 493 1204 4200 493 Size: 711 2996 2702 Fragments arranged by size: 2996 2702 711 BsaBI GATnn'nnATC Cuts at: 6403 6403 Size: 6409 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2394 4216 4598 5233 Size: 53 83 186 936 861 1822 382 635 Cuts at: 5233 275 Size: 1451 Fragments arranged by size: 1822 1451 936 861 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2922 4842 5240 5514 5661 6072 2922 Size: 1920 398 274 147 411 3259 Fragments arranged by size: 3259 1920 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6184 Fragments arranged by size: 6184 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2336 2930 1186 Size: 1150 594 4665 Fragments arranged by size: 4665 1150 594 BseYI C'CCAG_C Cuts at: 2380 2929 5612 6108 2380 Size: 549 2683 496 2681 Fragments arranged by size: 2683 2681 549 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2303 2352 1163 Size: 573 567 49 5220 Fragments arranged by size: 5220 573 567 49 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3859 4620 5648 1540 Size: 275 186 1858 761 1028 2301 Fragments arranged by size: 2301 1858 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2156 2703 3392 4470 4555 5626 2156 Size: 547 689 1078 85 1071 2939 Fragments arranged by size: 2939 1078 1071 689 547 85 BsmI GAATG_Cn' Cuts at: 3920 4013 3920 Size: 93 6316 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2156 2454 2666 2703 2937 3225 3321 Size: 399 366 298 212 37 234 288 96 Cuts at: 3321 3336 3392 3561 3728 4470 4555 5626 1391 Size: 15 56 169 167 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 366 298 288 234 212 169 167 96 85 56 37 15 BspEI T'CCGG_A Cuts at: 6072 6072 Size: 6409 BspHI T'CATG_A Cuts at: 4298 6028 4298 Size: 1730 4679 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2224 2325 3764 844 Size: 1380 101 1439 3489 Fragments arranged by size: 3489 1439 1380 101 BsrBI CCG'CTC Cuts at: 1394 1823 2068 2605 4296 1394 Size: 429 245 537 1691 3507 Fragments arranged by size: 3507 1691 537 429 245 BsrDI GCAATG_nn' Cuts at: 65 1248 4904 5078 65 Size: 1183 3656 174 1396 Fragments arranged by size: 3656 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2507 5053 5240 1700 Size: 110 697 2546 187 2869 Fragments arranged by size: 2869 2546 697 187 110 BsrGI T'GTAC_A Cuts at: 96 3468 96 Size: 3372 3037 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6409 BssSI C'ACGA_G Cuts at: 2744 4469 5481 2744 Size: 1725 1012 3672 Fragments arranged by size: 3672 1725 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3331 794 Size: 2537 3872 BstBI TT'CG_AA Cuts at: 5213 5213 Size: 6409 BstEII G'GTnAC_C Cuts at: 1184 3205 1184 Size: 2021 4388 BstXI CCAn_nnnn'nTGG Cuts at: 2788 3091 2788 Size: 303 6106 BstYI r'GATC_y Cuts at: 1972 3272 3739 4106 4491 4508 5960 6046 6058 Size: 1300 467 367 385 17 1452 86 12 Cuts at: 6058 6404 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1300 467 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2295 2436 513 Size: 552 939 291 141 4486 Fragments arranged by size: 4486 939 552 291 141 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5670 Fragments arranged by size: 5670 683 56 BtsI GCAGTG_nn' Cuts at: 3336 3925 4719 4739 3336 Size: 589 794 20 5006 Fragments arranged by size: 5006 794 589 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6409 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3381 3416 529 Size: 35 2817 35 3522 Fragments arranged by size: 3522 2817 35 35 DraI TTT'AAA Cuts at: 2848 3713 2848 Size: 865 5544 DraIII CAC_nnn'GTG Cuts at: 3331 3387 3331 Size: 56 6353 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5416 809 Size: 507 4100 1802 Fragments arranged by size: 4100 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2139 2292 2439 Size: 54 1425 50 275 18 309 153 147 Cuts at: 2439 2649 3081 3093 3402 3522 3856 4745 6374 Size: 210 432 12 309 120 334 889 1629 Cuts at: 6374 8 Size: 43 Fragments arranged by size: 1629 1425 889 432 334 309 309 275 210 153 147 120 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 3856 1537 Size: 275 2044 4090 Fragments arranged by size: 4090 2044 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2216 2603 4339 1353 Size: 863 387 1736 3423 Fragments arranged by size: 3423 1736 863 387 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3267 4996 5370 5516 387 Size: 1064 138 1678 1729 374 146 1280 Fragments arranged by size: 1729 1678 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2212 2534 2804 4472 5069 5856 6124 Size: 393 580 322 270 1668 597 787 268 Cuts at: 6124 1239 Size: 1524 Fragments arranged by size: 1668 1524 787 597 580 393 322 270 268 EcoO109I rG'GnC_Cy Cuts at: 2573 2687 2740 2933 2934 2573 Size: 114 53 193 1 6048 Fragments arranged by size: 6048 193 114 53 1 EcoRI G'AATT_C Cuts at: 3718 4096 3718 Size: 378 6031 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3154 3154 Size: 6409 FseI GG_CCGG'CC Cuts at: 2511 2511 Size: 6409 FspI TGC'GCA Cuts at: 2339 4915 2339 Size: 2576 3833 HaeII r_GCGC'y Cuts at: 1678 2018 2311 5556 1678 Size: 340 293 3245 2531 Fragments arranged by size: 3245 2531 340 293 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5050 5082 5124 5156 1373 Size: 32 135 32 3478 32 42 32 2626 Fragments arranged by size: 3478 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3637 3753 3999 669 Size: 988 281 1699 116 246 3079 Fragments arranged by size: 3079 1699 988 281 246 116 HindIII A'AGCT_T Cuts at: 748 748 Size: 6409 HpaI GTT'AAC Cuts at: 3999 3999 Size: 6409 KpnI G_GTAC'C Cuts at: 3734 3734 Size: 6409 MfeI C'AATT_G Cuts at: 4008 4008 Size: 6409 MluI A'CGCG_T Cuts at: 6285 6285 Size: 6409 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5523 5707 1305 Size: 4218 184 2007 Fragments arranged by size: 4218 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2141 3083 3095 3404 3524 Size: 54 1425 343 309 942 12 309 120 Cuts at: 3524 10 Size: 2895 Fragments arranged by size: 2895 1425 942 343 309 309 120 54 12 MslI CAynn'nnrTG Cuts at: 518 1166 2093 2135 2264 2441 2447 2789 3089 Size: 648 927 42 129 177 6 342 300 Cuts at: 3089 4367 4726 4885 518 Size: 1278 359 159 2042 Fragments arranged by size: 2042 1278 927 648 359 342 300 177 159 129 42 6 MspA1I CmG'CkG Cuts at: 1606 3466 3825 4502 5650 5895 1606 Size: 1860 359 677 1148 245 2120 Fragments arranged by size: 2120 1860 1148 677 359 245 NaeI GCC'GGC Cuts at: 2509 2509 Size: 6409 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4918 Fragments arranged by size: 4918 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6409 NgoMIV G'CCGG_C Cuts at: 2507 2507 Size: 6409 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6409 NotI GC'GGCC_GC Cuts at: 3856 3856 Size: 6409 NspI r_CATG'y Cuts at: 1132 1229 2269 2788 3767 5312 1132 Size: 97 1040 519 979 1545 2229 Fragments arranged by size: 2229 1545 1040 979 519 97 PasI CC'CwG_GG Cuts at: 1348 3225 1348 Size: 1877 4532 PciI A'CATG_T Cuts at: 5308 5308 Size: 6409 PflMI CCAn_nnn'nTGG Cuts at: 1108 2148 2221 1108 Size: 1040 73 5296 Fragments arranged by size: 5296 1040 73 PmeI GTTT'AAAC Cuts at: 3713 3713 Size: 6409 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4478 4510 5991 6023 4478 Size: 32 1481 32 4864 Fragments arranged by size: 4864 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2687 2740 2687 Size: 53 6356 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6409 PsiI TTA'TAA Cuts at: 3979 3979 Size: 6409 PspOMI G'GGCC_C Cuts at: 2933 2933 Size: 6409 PstI C_TGCA'G Cuts at: 830 2913 3761 830 Size: 2083 848 3478 Fragments arranged by size: 3478 2083 848 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6409 SalI G'TCGA_C Cuts at: 1936 3751 1936 Size: 1815 4594 SanDI GG'GwC_CC Cuts at: 2687 2687 Size: 6409 SbfI CC_TGCA'GG Cuts at: 3761 3761 Size: 6409 ScaI AGT'ACT Cuts at: 4657 6369 4657 Size: 1712 4697 SfcI C'TryA_G Cuts at: 826 1045 2909 3757 4892 5573 5764 826 Size: 219 1864 848 1135 681 191 1471 Fragments arranged by size: 1864 1471 1135 848 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6409 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6409 SmaI CCC'GGG Cuts at: 1872 3736 1872 Size: 1864 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2900 3563 4513 5414 5676 820 Size: 197 926 957 663 950 901 262 1553 Fragments arranged by size: 1553 957 950 926 901 663 262 197 SnaBI TAC'GTA Cuts at: 493 4200 493 Size: 3707 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6409 SphI G_CATG'C Cuts at: 1132 3767 1132 Size: 2635 3774 SspI AAT'ATT Cuts at: 5 52 4333 5 Size: 47 4281 2081 Fragments arranged by size: 4281 2081 47 StuI AGG'CCT Cuts at: 1517 3281 1517 Size: 1764 4645 StyI C'CwwG_G Cuts at: 513 1065 2004 2538 3848 513 Size: 552 939 534 1310 3074 Fragments arranged by size: 3074 1310 939 552 534 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2341 3031 4436 4453 4606 4791 5236 Size: 1255 761 690 1405 17 153 185 445 Cuts at: 5236 325 Size: 1498 Fragments arranged by size: 1498 1405 1255 761 690 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2247 3468 4655 Size: 274 80 33 51 1443 270 1221 1187 Cuts at: 4655 6367 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1221 1187 274 270 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2679 2711 6369 6401 2679 Size: 32 3658 32 2687 Fragments arranged by size: 3658 2687 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6409 XbaI T'CTAG_A Cuts at: 3745 3745 Size: 6409 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2443 3661 2443 Size: 1218 5191 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6409 XmaI C'CCGG_G Cuts at: 1870 3734 1870 Size: 1864 4545 XmnI GAAnn'nnTTC Cuts at: 4538 4538 Size: 6409 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4217 276 Size: 53 83 186 936 2683 2468 Fragments arranged by size: 2683 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI