################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I CelII ClaI CpoI DraI Eam1105I Eco47III EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI HincII HindII HindIII HinfI HpaI KpnI KspI MamI MfeI MluI MroI MseI MunI MvnI NheI NotI NruI NsiI NspV PacI PflFI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 1327 GTAGAC GT^[AC][GT]AC AcyI 926 GGCGCC G[GA]^CG[CT]C AflIII 1301 ACGTGT A^C[GA][CT]GT AocI 805 CCTGAGG CC^T[ACGT]AGG BamHI 706 GGATCC G^GATCC BbeI 926 GGCGCC GGCGC^C BfaI 487 CTAG C^TAG BsaHI 926 GGCGCC G[GA]^CG[CT]C BseRI 721 GAGGAG GAGGAG(10/8) BsiEI 1213 CGGCCG CG[GA][CT]^CG BstAPI 595 GCACTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 192 GGTGACC G^GT[ACGT]ACC BstPI 192 GGTGACC G^GT[ACGT]ACC Bsu36I 805 CCTGAGG CC^T[ACGT]AGG DrdI 802 GACCCTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1213 CGGCCG C^GGCCG EclXI 1213 CGGCCG C^GGCCG Eco52I 1213 CGGCCG C^GGCCG Eco81I 805 CCTGAGG CC^T[ACGT]AGG EcoO65I 192 GGTGACC G^GT[ACGT]ACC Hin1I 926 GGCGCC G[GA]^CG[CT]C KasI 926 GGCGCC G^GCGCC MaeI 487 CTAG C^TAG NaeI 1215 GCCGGC GCC^GGC NarI 926 GGCGCC GG^CGCC NdeI 1169 CATATG CA^TATG NgoMIV 1215 GCCGGC G^CCGGC NspI 1046 GCATGC [GA]CATG^[CT] PaeR7I 508 CTCGAG C^TCGAG PstI 1115 CTGCAG CTGCA^G SfaNI 1292 GCATC GCATC(5/9) SfcI 1115 CTGCAG C^T[GA][CT]AG SfoI 926 GGCGCC GGC^GCC SmaI 216 CCCGGG CCC^GGG SmlI 508 CTCGAG C^T[CT][GA]AG SphI 1046 GCATGC GCATG^C XcmI 1112 CCACTGCAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 508 CTCGAG C^TCGAG XmaCI 216 CCCGGG C^CCGGG XmaI 216 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AfaI 250 GTAC GT^AC 377 GTAC AvaI 216 CCCGGG C^[CT]CG[GA]G 508 CTCGAG BalI 989 TGGCCA TGG^CCA 1238 TGGCCA BbrPI 301 CACGTG CAC^GTG 1300 CACGTG BsaAI 301 CACGTG [CT]AC^GT[GA] 1300 CACGTG BsaI 454 GGTCTC GGTCTC(1/5) 637 GGTCTC BsgI 898 GTGCAG GTGCAG(16/14) 1005 GTGCAG BsmAI 455 GTCTC GTCTC(1/5) 638 GTCTC BsmFI 235 GTCCC GGGAC(10/14) 1011 GTCCC BsoBI 216 CCCGGG C^[CT]CG[GA]G 508 CTCGAG BsrFI 1215 GCCGGC [GA]^CCGG[CT] 1267 GCCGGT BstYI 86 GGATCT [GA]^GATC[CT] 706 GGATCC Cfr10I 1215 GCCGGC [GA]^CCGG[CT] 1267 GCCGGT DpnII 87 GATC ^GATC 707 GATC DraIII 298 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG 328 CACTCTGTG DsaI 831 CCATGG C^C[GA][CT]GG 992 CCATGG EarI 727 GAAGAG CTCTTC(1/4) 742 GAAGAG EcoNI 120 CCTTCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 444 CCTGGGTCAGG HphI 192 GGTGA GGTGA(8/7) 863 TCACC Ksp632I 727 GAAGAG CTCTTC(1/4) 742 GAAGAG MaeII 302 ACGT A^CGT 1301 ACGT MboI 87 GATC ^GATC 707 GATC MflI 86 GGATCT [GA]^GATC[CT] 706 GGATCC MluNI 989 TGGCCA TGG^CCA 1238 TGGCCA MscI 989 TGGCCA TGG^CCA 1238 TGGCCA NcoI 831 CCATGG C^CATGG 992 CCATGG NdeII 87 GATC ^GATC 707 GATC PflMI 36 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 327 CCACTCTGTGG PmaCI 301 CACGTG CAC^GTG 1300 CACGTG PmlI 301 CACGTG CAC^GTG 1300 CACGTG PpuMI 233 AGGTCCC [GA]G^G[AT]CC[CT] 1009 AGGTCCC RsaI 250 GTAC GT^AC 377 GTAC Sau3AI 87 GATC ^GATC 707 GATC TaqI 509 TCGA T^CGA 554 TCGA TthHB8I 509 TCGA T^CGA 554 TCGA Van91I 36 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 327 CCACTCTGTGG XhoII 86 GGATCT [GA]^GATC[CT] 706 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AluI AlwI AlwNI AvaII BanI BanII BbvI BcnI BglI BmyI BsaJI BsiYI BslI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MaeIII MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PvuII Sau96I ScrFI StyI TseI Tsp45I TspRI