(Linear) (Six-base) MAPSORT of: KIEE9541.seq Check: 3163 from: 1 to: 1344 KIEE9541.orf With 182 enzymes: SgfI * March 22, 2012 18:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 47 422 1344 Size: 47 375 922 Fragments arranged by size: 922 375 47 AccI GT'mk_AC Cuts at: 0 1328 1344 Size: 1328 16 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 602 1344 Size: 602 742 AflIII A'CryG_T Cuts at: 0 1301 1344 Size: 1301 43 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 295 1344 Size: 295 1049 AlwNI CAG_nnn'CTG Cuts at: 0 258 691 805 1344 Size: 258 433 114 539 Fragments arranged by size: 539 433 258 114 AvaI C'yCGr_G Cuts at: 0 216 508 1344 Size: 216 292 836 Fragments arranged by size: 836 292 216 BamHI G'GATC_C Cuts at: 0 706 1344 Size: 706 638 BanI G'GyrC_C Cuts at: 0 296 535 784 835 926 1108 1264 1270 Size: 296 239 249 51 91 182 156 6 Cuts at: 1270 1344 Size: 74 Fragments arranged by size: 296 249 239 182 156 91 74 51 6 BanII G_rGCy'C Cuts at: 0 67 685 1090 1255 1344 Size: 67 618 405 165 89 Fragments arranged by size: 618 405 165 89 67 BbeI G_GCGC'C Cuts at: 0 930 1344 Size: 930 414 BglI GCCn_nnn'nGGC Cuts at: 0 283 313 924 1344 Size: 283 30 611 420 Fragments arranged by size: 611 420 283 30 Bme1580I G_kGCm'C Cuts at: 0 274 465 517 597 1111 1344 Size: 274 191 52 80 514 233 Fragments arranged by size: 514 274 233 191 80 52 BmrI ACTGGGnnnn_n' Cuts at: 0 304 712 1344 Size: 304 408 632 Fragments arranged by size: 632 408 304 Bpu10I CC'TnA_GC Cuts at: 0 406 563 684 1084 1344 Size: 406 157 121 400 260 Fragments arranged by size: 406 400 260 157 121 BsaI GGTCTCn'nnnn_ Cuts at: 0 460 643 1344 Size: 460 183 701 Fragments arranged by size: 701 460 183 BsaAI yAC'GTr Cuts at: 0 303 1302 1344 Size: 303 999 42 Fragments arranged by size: 999 303 42 BsaHI Gr'CG_yC Cuts at: 0 927 1344 Size: 927 417 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 736 1344 Size: 736 608 BseYI C'CCAG_C Cuts at: 0 15 168 846 954 1041 1344 Size: 15 153 678 108 87 303 Fragments arranged by size: 678 303 153 108 87 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 919 1026 1344 Size: 919 107 318 Fragments arranged by size: 919 318 107 BsiEI CG_ry'CG Cuts at: 0 1216 1344 Size: 1216 128 Bsp1286I G_dGCh'C Cuts at: 0 67 274 465 517 597 685 1090 1111 Size: 67 207 191 52 80 88 405 21 Cuts at: 1111 1255 1344 Size: 144 89 Fragments arranged by size: 405 207 191 144 89 88 80 67 52 21 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 47 222 422 998 1344 Size: 47 175 200 576 346 Fragments arranged by size: 576 346 200 175 47 BsrDI GCAATG_nn' Cuts at: 0 371 754 1219 1344 Size: 371 383 465 125 Fragments arranged by size: 465 383 371 125 BsrFI r'CCGG_y Cuts at: 0 1215 1267 1344 Size: 1215 52 77 Fragments arranged by size: 1215 77 52 BstAPI GCAn_nnn'nTGC Cuts at: 0 601 1344 Size: 601 743 BstEII G'GTnAC_C Cuts at: 0 192 1344 Size: 192 1152 BstXI CCAn_nnnn'nTGG Cuts at: 0 131 246 1248 1344 Size: 131 115 1002 96 Fragments arranged by size: 1002 131 115 96 BstYI r'GATC_y Cuts at: 0 86 706 1344 Size: 86 620 638 Fragments arranged by size: 638 620 86 Bsu36I CC'TnA_GG Cuts at: 0 806 1344 Size: 806 538 BtgI C'CryG_G Cuts at: 0 831 992 1344 Size: 831 161 352 Fragments arranged by size: 831 352 161 BtsI GCAGTG_nn' Cuts at: 0 966 1112 1344 Size: 966 146 232 Fragments arranged by size: 966 232 146 DraIII CAC_nnn'GTG Cuts at: 0 303 333 1344 Size: 303 30 1011 Fragments arranged by size: 1011 303 30 DrdI GACnn_nn'nnGTC Cuts at: 0 808 1344 Size: 808 536 EaeI y'GGCC_r Cuts at: 0 989 1213 1238 1344 Size: 989 224 25 106 Fragments arranged by size: 989 224 106 25 EagI C'GGCC_G Cuts at: 0 1213 1344 Size: 1213 131 EarI CTCTTCn'nnn_ Cuts at: 0 722 737 1344 Size: 722 15 607 Fragments arranged by size: 722 607 15 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 602 1344 Size: 602 742 EcoNI CCTnn'n_nnAGG Cuts at: 0 124 448 1344 Size: 124 324 896 Fragments arranged by size: 896 324 124 EcoO109I rG'GnC_Cy Cuts at: 0 234 688 1010 1058 1344 Size: 234 454 322 48 286 Fragments arranged by size: 454 322 286 234 48 HaeII r_GCGC'y Cuts at: 0 169 526 930 1344 Size: 169 357 404 414 Fragments arranged by size: 414 404 357 169 KasI G'GCGC_C Cuts at: 0 926 1344 Size: 926 418 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 112 1344 Size: 112 1232 MscI TGG'CCA Cuts at: 0 991 1240 1344 Size: 991 249 104 Fragments arranged by size: 991 249 104 MslI CAynn'nnrTG Cuts at: 0 244 997 1246 1344 Size: 244 753 249 98 Fragments arranged by size: 753 249 244 98 MspA1I CmG'CkG Cuts at: 0 622 819 1231 1344 Size: 622 197 412 113 Fragments arranged by size: 622 412 197 113 NaeI GCC'GGC Cuts at: 0 1217 1344 Size: 1217 127 NarI GG'CG_CC Cuts at: 0 927 1344 Size: 927 417 NcoI C'CATG_G Cuts at: 0 831 992 1344 Size: 831 161 352 Fragments arranged by size: 831 352 161 NdeI CA'TA_TG Cuts at: 0 1170 1344 Size: 1170 174 NgoMIV G'CCGG_C Cuts at: 0 1215 1344 Size: 1215 129 NspI r_CATG'y Cuts at: 0 1050 1344 Size: 1050 294 PflMI CCAn_nnn'nTGG Cuts at: 0 42 333 1344 Size: 42 291 1011 Fragments arranged by size: 1011 291 42 PmlI CAC'GTG Cuts at: 0 303 1302 1344 Size: 303 999 42 Fragments arranged by size: 999 303 42 PpuMI rG'GwC_Cy Cuts at: 0 234 1010 1344 Size: 234 776 334 Fragments arranged by size: 776 334 234 PspXI vC'TCGA_Gb Cuts at: 0 508 1344 Size: 508 836 PstI C_TGCA'G Cuts at: 0 1119 1344 Size: 1119 225 PvuII CAG'CTG Cuts at: 0 622 819 1231 1344 Size: 622 197 412 113 Fragments arranged by size: 622 412 197 113 SfcI C'TryA_G Cuts at: 0 1115 1344 Size: 1115 229 SfoI GGC'GCC Cuts at: 0 928 1344 Size: 928 416 SmaI CCC'GGG Cuts at: 0 218 1344 Size: 218 1126 SmlI C'TyrA_G Cuts at: 0 508 1344 Size: 508 836 SphI G_CATG'C Cuts at: 0 1050 1344 Size: 1050 294 StyI C'CwwG_G Cuts at: 0 353 831 992 1344 Size: 353 478 161 352 Fragments arranged by size: 478 353 352 161 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 24 81 1134 1344 Size: 24 57 1053 210 Fragments arranged by size: 1053 210 57 24 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1064 1096 1344 Size: 1064 32 248 Fragments arranged by size: 1064 248 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1119 1344 Size: 1119 225 XhoI C'TCGA_G Cuts at: 0 508 1344 Size: 508 836 XmaI C'CCGG_G Cuts at: 0 216 1344 Size: 216 1128 Enzymes that do cut: AarI AccI AcuI AflIII AlfI AlwNI AvaI BamHI BanI BanII BbeI BglI Bme1580I BmrI Bpu10I BsaI BsaAI BsaHI BseRI BseYI BsgI BsiEI Bsp1286I BspMI BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I HaeII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PflMI PmlI PpuMI PspXI PstI PvuII SfcI SfoI SmaI SmlI SphI StyI TaqII TstI XcmI XhoI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI BpmI BpuEI BsaBI BsaWI BsaXI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstBI BstZ17I BtgZI ClaI CspCI DraI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI TatI Tth111I XbaI XmnI ZraI