Restriction map for
pFN21AE3140
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BbvCI BcgI BclI BfrI
BlnI BlpI Bpu1102I BsaAI BsaHI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MaeIII MfeI MluI MroI MseI
MslI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 457 GTCTAC GT^[AC][GT]AC
AcsI 342 GAATTC [GA]^AATT[CT]
AlwNI 759 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 342 GAATTC [GA]^AATT[CT]
AspHI 743 GTGCTC G[AT]GC[AT]^C
BalI 557 TGGCCA TGG^CCA
BamHI 401 GGATCC G^GATCC
BanI 319 GGTGCC G^G[CT][GA]CC
BciVI 587 GTATCC GTATCC(6/5)
BfaI 938 CTAG C^TAG
BglII 395 AGATCT A^GATCT
BsaBI 396 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 671 GAGACC GGTCTC(1/5)
BsaWI 596 ACCGGA [AT]^CCGG[AT]
BseRI 874 GAGGAG GAGGAG(10/8)
BsiHKAI 743 GTGCTC G[AT]GC[AT]^C
BsmAI 671 GAGAC GTCTC(1/5)
BsrBI 53 GAGCGG CCGCTC(-3/-3)
BsrDI 207 CATTGC GCAATG(2/0)
BsrI 675 CCAGT ACTGG(1/-1)
BstBI 260 TTCGAA TT^CGAA
CfoI 253 GCGC GCG^C
DraIII 334 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 283 GACCTCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 560 CCATGG C^C[GA][CT]GG
EcoRI 342 GAATTC G^AATTC
HhaI 253 GCGC GCG^C
HinP1I 253 GCGC G^CGC
MaeI 938 CTAG C^TAG
MamI 396 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 557 TGGCCA TGG^CCA
MscI 557 TGGCCA TGG^CCA
NcoI 560 CCATGG C^CATGG
NspV 260 TTCGAA TT^CGAA
SfuI 260 TTCGAA TT^CGAA
SmaI 65 CCCGGG CCC^GGG
Tsp509I 343 AATT ^AATT
TspRI 1016 CACTG CA[GC]TG[ACGT][ACGT]^
XmaCI 65 CCCGGG C^CCGGG
XmaI 65 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AvaI 65 CCCGGG C^[CT]CG[GA]G
473 CTCGGG
BanII 70 GGGCTC G[GA]GC[CT]^C
470 GGGCTC
BbsI 795 GAAGAC GAAGAC(2/6)
844 GAAGAC
BglI 149 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
301 GCCCAGAAGGC
BpmI 160 CTCCAG CTGGAG(16/14)
607 CTCCAG
BpuAI 795 GAAGAC GAAGAC(2/6)
844 GAAGAC
BsoBI 65 CCCGGG C^[CT]CG[GA]G
473 CTCGGG
BspMI 440 ACCTGC ACCTGC(4/8)
799 ACCTGC
BstXI 303 CCAGAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
692 CCATTTGCCTGG
EaeI 35 TGGCCG [CT]^GGCC[GA]
557 TGGCCA
EarI 947 CTCTTC CTCTTC(1/4)
1029 CTCTTC
FauI 18 CCCGC CCCGC(4/6)
729 GCGGG
HphI 880 GGTGA GGTGA(8/7)
183 TCACC
Ksp632I 947 CTCTTC CTCTTC(1/4)
1029 CTCTTC
PstI 8 CTGCAG CTGCA^G
198 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BcnI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MboI MboII
MflI MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PpuMI RsaI Sau3AI Sau96I ScrFI
SfcI SmlI StyI TaqI TfiI
TseI TthHB8I XhoII