(Circular) (Six-base) MAPSORT of: pFN21AE3140.seq Check: 9490 from: 1 to: 5755 pFN21AE3140 5755 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3565 278 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2463 3098 1937 Size: 526 635 4594 Fragments arranged by size: 4594 635 526 Acc65I G'GTAC_C Cuts at: 3076 3076 Size: 5755 AclI AA'CG_TT Cuts at: 1563 3882 4255 1563 Size: 2319 373 3063 Fragments arranged by size: 3063 2319 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3818 5202 5470 1632 Size: 2186 1384 268 1917 Fragments arranged by size: 2186 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5558 AflIII A'CryG_T Cuts at: 1203 4654 5631 1203 Size: 3451 977 1327 Fragments arranged by size: 3451 1327 977 AgeI A'CCGG_T Cuts at: 4586 4586 Size: 5755 AhdI GACnn_n'nnGTC Cuts at: 4484 4484 Size: 5755 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5755 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5755 AlwNI CAG_nnn'CTG Cuts at: 2769 3498 5070 2769 Size: 729 1572 3454 Fragments arranged by size: 3454 1572 729 ApaLI G'TGCA_C Cuts at: 3812 4968 3812 Size: 1156 4599 ApoI r'AATT_y Cuts at: 1493 2347 3064 3290 3442 5551 1493 Size: 854 717 226 152 2109 1697 Fragments arranged by size: 2109 1697 854 717 226 152 AseI AT'TA_AT Cuts at: 160 1032 4309 160 Size: 872 3277 1606 Fragments arranged by size: 3277 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 AvaI C'yCGr_G Cuts at: 1870 1943 2070 2478 3080 1870 Size: 73 127 408 602 4545 Fragments arranged by size: 4545 602 408 127 73 BamHI G'GATC_C Cuts at: 2406 3085 3452 2406 Size: 679 367 4709 Fragments arranged by size: 4709 679 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2324 3076 4531 618 Size: 325 217 627 537 752 1455 1842 Fragments arranged by size: 1842 1455 752 627 537 325 217 BanII G_rGCy'C Cuts at: 1391 2079 2479 3074 1391 Size: 688 400 595 4072 Fragments arranged by size: 4072 688 595 400 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2807 2856 928 Size: 971 908 49 3827 Fragments arranged by size: 3827 971 908 49 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3946 3980 1903 Size: 34 50 34 1925 34 3678 Fragments arranged by size: 3678 1925 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2603 3647 4857 2603 Size: 1044 1210 3501 Fragments arranged by size: 3501 1210 1044 BclI T'GATC_A Cuts at: 1548 1548 Size: 5755 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2160 2312 4366 Size: 107 122 71 631 605 488 152 2054 Cuts at: 4366 136 Size: 1525 Fragments arranged by size: 2054 1525 631 605 488 152 122 107 71 BglII A'GATC_T Cuts at: 2400 5750 2400 Size: 3350 2405 BlpI GC'TnA_GC Cuts at: 3172 3172 Size: 5755 Bme1580I G_kGCm'C Cuts at: 1790 2455 3816 4972 1790 Size: 665 1361 1156 2573 Fragments arranged by size: 2573 1361 1156 665 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5755 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3486 4444 442 Size: 416 53 83 399 24 2069 958 1753 Fragments arranged by size: 2069 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5755 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2452 2452 Size: 5755 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2150 2597 4415 1239 Size: 911 447 1818 2579 Fragments arranged by size: 2579 1818 911 447 Bpu10I CC'TnA_GC Cuts at: 2974 2974 Size: 5755 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2170 2276 2853 3880 4745 5043 2170 Size: 106 577 1027 865 298 2882 Fragments arranged by size: 2882 1027 865 577 298 106 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2670 4418 882 Size: 617 1171 1748 2219 Fragments arranged by size: 2219 1748 1171 617 BsaAI yAC'GTr Cuts at: 493 1204 3546 493 Size: 711 2342 2702 Fragments arranged by size: 2702 2342 711 BsaBI GATnn'nnATC Cuts at: 2405 5749 2405 Size: 3344 2411 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3562 3944 4579 275 Size: 53 83 186 936 2029 382 635 1451 Fragments arranged by size: 2029 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2601 4188 4586 4860 5007 5418 2601 Size: 1587 398 274 147 411 2938 Fragments arranged by size: 2938 1587 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5530 Fragments arranged by size: 5530 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2894 1186 Size: 1708 4047 BseYI C'CCAG_C Cuts at: 2227 2980 4958 5454 2227 Size: 753 1978 496 2528 Fragments arranged by size: 2528 1978 753 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5182 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3205 3966 4994 1540 Size: 275 186 1204 761 1028 2301 Fragments arranged by size: 2301 1204 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2752 3816 3901 4972 2752 Size: 1064 85 1071 3535 Fragments arranged by size: 3535 1071 1064 85 BsmI GAATG_Cn' Cuts at: 3266 3359 3266 Size: 93 5662 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2455 2479 2752 3074 3816 3901 Size: 399 289 376 24 273 322 742 85 Cuts at: 3901 4972 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 376 322 289 273 85 24 BspEI T'CCGG_A Cuts at: 5418 5418 Size: 5755 BspHI T'CATG_A Cuts at: 3644 5374 3644 Size: 1730 4025 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2454 2813 3110 844 Size: 1610 359 297 3489 Fragments arranged by size: 3489 1610 359 297 BsrBI CCG'CTC Cuts at: 1394 1823 2060 3642 1394 Size: 429 237 1582 3507 Fragments arranged by size: 3507 1582 429 237 BsrDI GCAATG_nn' Cuts at: 65 1248 2211 4250 4424 65 Size: 1183 963 2039 174 1396 Fragments arranged by size: 2039 1396 1183 963 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4399 4586 1700 Size: 110 2589 187 2869 Fragments arranged by size: 2869 2589 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5755 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5755 BssSI C'ACGA_G Cuts at: 3815 4827 3815 Size: 1012 4743 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5755 BstBI TT'CG_AA Cuts at: 2266 4559 2266 Size: 2293 3462 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5755 BstXI CCAn_nnnn'nTGG Cuts at: 2315 2704 2315 Size: 389 5366 BstYI r'GATC_y Cuts at: 1972 2400 2406 3013 3085 3452 3837 3854 5306 Size: 428 6 607 72 367 385 17 1452 Cuts at: 5306 5392 5404 5750 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 607 428 385 367 346 86 72 17 12 6 BtgI C'CryG_G Cuts at: 513 1065 2565 513 Size: 552 1500 3703 Fragments arranged by size: 3703 1500 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5016 Fragments arranged by size: 5016 683 56 BtsI GCAGTG_nn' Cuts at: 3271 4065 4085 3271 Size: 794 20 4941 Fragments arranged by size: 4941 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5755 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2947 2982 529 Size: 35 2383 35 3302 Fragments arranged by size: 3302 2383 35 35 DraI TTT'AAA Cuts at: 3059 3059 Size: 5755 DraIII CAC_nnn'GTG Cuts at: 2344 2344 Size: 5755 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2294 4762 809 Size: 507 978 2468 1802 Fragments arranged by size: 2468 1802 978 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2562 3202 Size: 54 1425 50 275 18 210 522 640 Cuts at: 3202 4091 5720 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 640 522 275 210 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3202 1537 Size: 275 1390 4090 Fragments arranged by size: 4090 1390 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2958 3040 3685 1353 Size: 1605 82 645 3423 Fragments arranged by size: 3423 1605 645 82 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4342 4716 4862 387 Size: 1064 138 2753 374 146 1280 Fragments arranged by size: 2753 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2150 2597 3818 4415 5202 5470 1239 Size: 393 518 447 1221 597 787 268 1524 Fragments arranged by size: 1524 1221 787 597 518 447 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2180 2535 2920 2180 Size: 355 385 5015 Fragments arranged by size: 5015 385 355 EcoO109I rG'GnC_Cy Cuts at: 2161 2296 2837 2925 2161 Size: 135 541 88 4991 Fragments arranged by size: 4991 541 135 88 EcoRI G'AATT_C Cuts at: 2347 3064 3442 2347 Size: 717 378 4660 Fragments arranged by size: 4660 717 378 FspI TGC'GCA Cuts at: 4261 4261 Size: 5755 HaeII r_GCGC'y Cuts at: 1678 4902 1678 Size: 3224 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4396 4428 4470 4502 1373 Size: 32 135 32 2824 32 42 32 2626 Fragments arranged by size: 2824 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3099 3345 669 Size: 988 281 1161 246 3079 Fragments arranged by size: 3079 1161 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5755 HpaI GTT'AAC Cuts at: 3345 3345 Size: 5755 KpnI G_GTAC'C Cuts at: 3080 3080 Size: 5755 MfeI C'AATT_G Cuts at: 3354 3354 Size: 5755 MluI A'CGCG_T Cuts at: 5631 5631 Size: 5755 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4869 5053 1305 Size: 3564 184 2007 Fragments arranged by size: 3564 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2564 10 Size: 54 1425 343 732 3201 Fragments arranged by size: 3201 1425 732 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3713 4072 4231 518 Size: 648 2547 359 159 2042 Fragments arranged by size: 2547 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2045 2330 2617 2769 3171 3848 4996 5241 Size: 439 285 287 152 402 677 1148 245 Cuts at: 5241 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 439 402 287 285 245 152 NcoI C'CATG_G Cuts at: 513 1065 2565 513 Size: 552 1500 3703 Fragments arranged by size: 3703 1500 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5755 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5755 NotI GC'GGCC_GC Cuts at: 3202 3202 Size: 5755 NspI r_CATG'y Cuts at: 1132 1229 3113 4658 1132 Size: 97 1884 1545 2229 Fragments arranged by size: 2229 1884 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5755 PciI A'CATG_T Cuts at: 4654 4654 Size: 5755 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5755 PmeI GTTT'AAAC Cuts at: 3059 3059 Size: 5755 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3824 3856 5337 5369 3824 Size: 32 1481 32 4210 Fragments arranged by size: 4210 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2296 2837 2925 2296 Size: 541 88 5126 Fragments arranged by size: 5126 541 88 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5755 PsiI TTA'TAA Cuts at: 3325 3325 Size: 5755 PstI C_TGCA'G Cuts at: 830 2017 2207 3107 830 Size: 1187 190 900 3478 Fragments arranged by size: 3478 1187 900 190 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5755 SalI G'TCGA_C Cuts at: 1936 3097 1936 Size: 1161 4594 SbfI CC_TGCA'GG Cuts at: 3107 3107 Size: 5755 ScaI AGT'ACT Cuts at: 4003 5715 4003 Size: 1712 4043 SfcI C'TryA_G Cuts at: 826 1045 2013 2203 2331 3103 4238 4919 5110 Size: 219 968 190 128 772 1135 681 191 Cuts at: 5110 826 Size: 1471 Fragments arranged by size: 1471 1135 968 772 681 219 191 190 128 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5755 SmaI CCC'GGG Cuts at: 1872 2072 3082 1872 Size: 200 1010 4545 Fragments arranged by size: 4545 1010 200 SmlI C'TyrA_G Cuts at: 820 1017 1943 2149 2291 2868 3859 4760 5022 Size: 197 926 206 142 577 991 901 262 Cuts at: 5022 820 Size: 1553 Fragments arranged by size: 1553 991 926 901 577 262 206 197 142 SnaBI TAC'GTA Cuts at: 493 3546 493 Size: 3053 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5755 SphI G_CATG'C Cuts at: 1132 3113 1132 Size: 1981 3774 SspI AAT'ATT Cuts at: 5 52 3679 5 Size: 47 3627 2081 Fragments arranged by size: 3627 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5755 StyI C'CwwG_G Cuts at: 513 1065 2511 2565 2900 3194 513 Size: 552 1446 54 335 294 3074 Fragments arranged by size: 3074 1446 552 335 294 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2205 3782 3799 3952 4137 4582 325 Size: 1255 625 1577 17 153 185 445 1498 Fragments arranged by size: 1577 1498 1255 625 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2335 4001 5713 Size: 274 80 33 51 1443 358 1666 1712 Cuts at: 5713 96 Size: 138 Fragments arranged by size: 1712 1666 1443 358 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5715 5747 5715 Size: 32 5723 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5755 XbaI T'CTAG_A Cuts at: 3091 3091 Size: 5755 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5755 XmaI C'CCGG_G Cuts at: 1870 2070 3080 1870 Size: 200 1010 4545 Fragments arranged by size: 4545 1010 200 XmnI GAAnn'nnTTC Cuts at: 3884 3884 Size: 5755 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3563 276 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI