(Linear) (Six-base) MAPSORT of: KIEE3140.seq Check: 6341 from: 1 to: 1050 KIEE3140.orf With 182 enzymes: SgfI * December 27, 2011 16:33 .. AccI GT'mk_AC Cuts at: 0 458 1050 Size: 458 592 AlwNI CAG_nnn'CTG Cuts at: 0 764 1050 Size: 764 286 ApoI r'AATT_y Cuts at: 0 342 1050 Size: 342 708 AvaI C'yCGr_G Cuts at: 0 65 473 1050 Size: 65 408 577 Fragments arranged by size: 577 408 65 BamHI G'GATC_C Cuts at: 0 401 1050 Size: 401 649 BanI G'GyrC_C Cuts at: 0 319 1050 Size: 319 731 BanII G_rGCy'C Cuts at: 0 74 474 1050 Size: 74 400 576 Fragments arranged by size: 576 400 74 BbsI GAAGACnn'nnnn_ Cuts at: 0 802 851 1050 Size: 802 49 199 Fragments arranged by size: 802 199 49 BciVI GTATCCnnnnn_n' Cuts at: 0 598 1050 Size: 598 452 BglI GCCn_nnn'nGGC Cuts at: 0 155 307 1050 Size: 155 152 743 Fragments arranged by size: 743 155 152 BglII A'GATC_T Cuts at: 0 395 1050 Size: 395 655 Bme1580I G_kGCm'C Cuts at: 0 450 1050 Size: 450 600 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 447 1050 Size: 447 603 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 145 592 1050 Size: 145 447 458 Fragments arranged by size: 458 447 145 Bpu10I CC'TnA_GC Cuts at: 0 969 1050 Size: 969 81 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 165 271 848 1050 Size: 165 106 577 202 Fragments arranged by size: 577 202 165 106 BsaI GGTCTCn'nnnn_ Cuts at: 0 665 1050 Size: 665 385 BsaBI GATnn'nnATC Cuts at: 0 400 1050 Size: 400 650 BsaWI w'CCGG_w Cuts at: 0 596 1050 Size: 596 454 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 889 1050 Size: 889 161 BseYI C'CCAG_C Cuts at: 0 222 975 1050 Size: 222 753 75 Fragments arranged by size: 753 222 75 BsiHKAI G_wGCw'C Cuts at: 0 747 1050 Size: 747 303 Bsp1286I G_dGCh'C Cuts at: 0 74 450 474 747 1050 Size: 74 376 24 273 303 Fragments arranged by size: 376 303 273 74 24 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 449 808 1050 Size: 449 359 242 Fragments arranged by size: 449 359 242 BsrBI CCG'CTC Cuts at: 0 55 1050 Size: 55 995 BsrDI GCAATG_nn' Cuts at: 0 206 1050 Size: 206 844 BstBI TT'CG_AA Cuts at: 0 261 1050 Size: 261 789 BstXI CCAn_nnnn'nTGG Cuts at: 0 310 699 1050 Size: 310 389 351 Fragments arranged by size: 389 351 310 BstYI r'GATC_y Cuts at: 0 395 401 1008 1050 Size: 395 6 607 42 Fragments arranged by size: 607 395 42 6 BtgI C'CryG_G Cuts at: 0 560 1050 Size: 560 490 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 942 977 1050 Size: 942 35 73 Fragments arranged by size: 942 73 35 DraIII CAC_nnn'GTG Cuts at: 0 339 1050 Size: 339 711 DrdI GACnn_nn'nnGTC Cuts at: 0 289 1050 Size: 289 761 EaeI y'GGCC_r Cuts at: 0 35 557 1050 Size: 35 522 493 Fragments arranged by size: 522 493 35 EarI CTCTTCn'nnn_ Cuts at: 0 953 1035 1050 Size: 953 82 15 Fragments arranged by size: 953 82 15 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 145 592 1050 Size: 145 447 458 Fragments arranged by size: 458 447 145 EcoNI CCTnn'n_nnAGG Cuts at: 0 175 530 915 1050 Size: 175 355 385 135 Fragments arranged by size: 385 355 175 135 EcoO109I rG'GnC_Cy Cuts at: 0 156 291 832 920 1050 Size: 156 135 541 88 130 Fragments arranged by size: 541 156 135 130 88 EcoRI G'AATT_C Cuts at: 0 342 1050 Size: 342 708 MscI TGG'CCA Cuts at: 0 559 1050 Size: 559 491 MspA1I CmG'CkG Cuts at: 0 40 325 612 764 1050 Size: 40 285 287 152 286 Fragments arranged by size: 287 286 285 152 40 NcoI C'CATG_G Cuts at: 0 560 1050 Size: 560 490 PpuMI rG'GwC_Cy Cuts at: 0 291 832 920 1050 Size: 291 541 88 130 Fragments arranged by size: 541 291 130 88 PstI C_TGCA'G Cuts at: 0 12 202 1050 Size: 12 190 848 Fragments arranged by size: 848 190 12 SfcI C'TryA_G Cuts at: 0 8 198 326 1050 Size: 8 190 128 724 Fragments arranged by size: 724 190 128 8 SmaI CCC'GGG Cuts at: 0 67 1050 Size: 67 983 SmlI C'TyrA_G Cuts at: 0 144 286 863 1050 Size: 144 142 577 187 Fragments arranged by size: 577 187 144 142 StyI C'CwwG_G Cuts at: 0 506 560 895 1050 Size: 506 54 335 155 Fragments arranged by size: 506 335 155 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 200 1050 Size: 200 850 TatI w'GTAC_w Cuts at: 0 330 1050 Size: 330 720 XmaI C'CCGG_G Cuts at: 0 65 1050 Size: 65 985 Enzymes that do cut: AccI AlwNI ApoI AvaI BamHI BanI BanII BbsI BciVI BglI BglII Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BseRI BseYI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BstBI BstXI BstYI BtgI CspCI DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI MscI MspA1I NcoI PpuMI PstI SfcI SmaI SmlI StyI TaqII TatI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BclI BfrBI BlpI BmgBI BmrI BmtI BsaAI BsaHI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI DraI EagI EciI EcoICRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmnI ZraI