Restriction map for
pFN21AE2043
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# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AccI AclI AflIII Aor51HI
AscI AseI AsnI AvrII BciVI
BclI BglI BglII BlnI BsiWI
BsmBI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstZ17I
ClaI CpoI DraI DrdI Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HpaI KspI MfeI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SfiI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 686 GGTACC G^GTACC
AccIII 1916 TCCGGA T^CCGGA
AcsI 1235 GAATTT [GA]^AATT[CT]
AflII 211 CTTAAG C^TTAAG
AgeI 416 ACCGGT A^CCGGT
AhdI 1488 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1627 GTGCAC G^TGCAC
ApaLI 1627 GTGCAC G^TGCAC
ApoI 1235 GAATTT [GA]^AATT[CT]
Asp700I 1869 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 686 GGTACC G^GTACC
AspEI 1488 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 268 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 703 GGCGCC GGCGC^C
BbrPI 1273 CACGTG CAC^GTG
BfrI 211 CTTAAG C^TTAAG
BlpI 643 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 643 GCTGAGC GC^T[ACGT]AGC
BsaAI 1273 CACGTG [CT]AC^GT[GA]
BsaBI 1767 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1916 TCCGGA T^CCGGA
BsmI 1920 GAATGC GAATGC(1/-1)
BspEI 1916 TCCGGA T^CCGGA
BstBI 820 TTCGAA TT^CGAA
CelII 643 GCTGAGC GC^T[ACGT]AGC
DraIII 1587 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 336 CGGCCG C^GGCCG
Eam1105I 1488 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 336 CGGCCG C^GGCCG
Eco52I 336 CGGCCG C^GGCCG
HincII 514 GTTGAC GT[CT]^[GA]AC
HindII 514 GTTGAC GT[CT]^[GA]AC
HindIII 1159 AAGCTT A^AGCTT
KasI 703 GGCGCC G^GCGCC
KpnI 686 GGTACC GGTAC^C
MamI 1767 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1916 TCCGGA T^CCGGA
NarI 703 GGCGCC GG^CGCC
NcoI 938 CCATGG C^CATGG
NspI 1934 ACATGC [GA]CATG^[CT]
NspV 820 TTCGAA TT^CGAA
PflFI 268 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 416 ACCGGT A^CCGGT
PmaCI 1273 CACGTG CAC^GTG
PmlI 1273 CACGTG CAC^GTG
PpuMI 353 AGGACCC [GA]G^G[AT]CC[CT]
SapI 388 GAAGAGC GCTCTTC(1/4)
SfoI 703 GGCGCC GGC^GCC
SfuI 820 TTCGAA TT^CGAA
TfiI 1430 GAATC G^A[AT]TC
Tsp45I 1585 GTCAC ^GT[GC]AC
Tth111I 268 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 597 TCTAGA T^CTAGA
XmnI 1869 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 19 CGCG CG^CG
649 CGCG
AlwNI 361 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
880 CAGCACCTG
AocI 358 CCTCAGG CC^T[ACGT]AGG
1855 CCTAAGG
AspHI 1254 GAGCAC G[AT]GC[AT]^C
1627 GTGCAC
AvaI 103 CTCGGG C^[CT]CG[GA]G
1562 CTCGGG
AviII 194 TGCGCA TGC^GCA
1040 TGCGCA
BamHI 970 GGATCC G^GATCC
1002 GGATCC
BbsI 502 GAAGAC GAAGAC(2/6)
1368 GTCTTC
BbvCI 720 GCTGAGG CCTCAGC(-5/-2)
1102 GCTGAGG
BcgI 1679 CGATGCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
812 GCACACTGTTCG
BpuAI 502 GAAGAC GAAGAC(2/6)
1368 GTCTTC
BsaI 4 GGTCTC GGTCTC(1/5)
1221 GGTCTC
BsiEI 336 CGGCCG CG[GA][CT]^CG
1344 CGACCG
BsiHKAI 1254 GAGCAC G[AT]GC[AT]^C
1627 GTGCAC
BsoBI 103 CTCGGG C^[CT]CG[GA]G
1562 CTCGGG
BstUI 19 CGCG CG^CG
649 CGCG
Bsu36I 358 CCTCAGG CC^T[ACGT]AGG
1855 CCTAAGG
DsaI 713 CCGTGG C^C[GA][CT]GG
938 CCATGG
EarI 388 GAAGAG CTCTTC(1/4)
397 GAAGAG
Eco81I 358 CCTCAGG CC^T[ACGT]AGG
1855 CCTAAGG
EcoNI 207 CCTCCTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1896 CCTGGATAAGG
FspI 194 TGCGCA TGC^GCA
1040 TGCGCA
Ksp632I 388 GAAGAG CTCTTC(1/4)
397 GAAGAG
MaeIII 287 GTTAC ^GT[ACGT]AC
1585 GTCAC
MslI 15 CATCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1110 CATTGTTGTG
MvnI 19 CGCG CG^CG
649 CGCG
PflMI 1028 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1772 CCATCACTTGG
Van91I 1028 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1772 CCATCACTTGG
XcmI 690 CCAGTTTCTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1208 CCAGGACTGGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
ApaI AvaII BalI BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaJI BsaWI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI StyI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII