(Circular) (Six-base) MAPSORT of: pFN21AE2043.seq Check: 2008 from: 1 to: 6691 pFN21AE2043 6691 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3189 3617 3189 Size: 428 6263 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4501 278 Size: 53 83 186 936 2965 2468 Fragments arranged by size: 2965 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4034 1937 Size: 2097 4594 Acc65I G'GTAC_C Cuts at: 2691 4012 2691 Size: 1321 5370 AclI AA'CG_TT Cuts at: 1563 4818 5191 1563 Size: 3255 373 3063 Fragments arranged by size: 3255 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2352 3893 4754 6138 6406 1632 Size: 720 1541 861 1384 268 1917 Fragments arranged by size: 1917 1541 1384 861 720 268 AflII C'TTAA_G Cuts at: 820 1017 2216 820 Size: 197 1199 5295 Fragments arranged by size: 5295 1199 197 AflIII A'CryG_T Cuts at: 1203 5590 6567 1203 Size: 4387 977 1327 Fragments arranged by size: 4387 1327 977 AgeI A'CCGG_T Cuts at: 2421 5522 2421 Size: 3101 3590 AhdI GACnn_n'nnGTC Cuts at: 3498 5420 3498 Size: 1922 4769 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6691 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2910 1184 Size: 1726 4965 AlwNI CAG_nnn'CTG Cuts at: 2371 2890 4434 6006 2371 Size: 519 1544 1572 3056 Fragments arranged by size: 3056 1572 1544 519 ApaI G_GGCC'C Cuts at: 2261 3535 3854 2261 Size: 1274 319 5098 Fragments arranged by size: 5098 1274 319 ApaLI G'TGCA_C Cuts at: 3632 4748 5904 3632 Size: 1116 1156 4419 Fragments arranged by size: 4419 1156 1116 ApoI r'AATT_y Cuts at: 1493 3240 4000 4226 4378 6487 1493 Size: 1747 760 226 152 2109 1697 Fragments arranged by size: 2109 1747 1697 760 226 152 AseI AT'TA_AT Cuts at: 160 1032 5245 160 Size: 872 4213 1606 Fragments arranged by size: 4213 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6691 AvaI C'yCGr_G Cuts at: 1870 1943 2108 3567 4016 1870 Size: 73 165 1459 449 4545 Fragments arranged by size: 4545 1459 449 165 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2153 2186 2153 Size: 33 6658 BamHI G'GATC_C Cuts at: 2975 3007 4021 4388 2975 Size: 32 1014 367 5278 Fragments arranged by size: 5278 1014 367 32 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2208 2336 2691 2708 3135 Size: 325 217 627 421 128 355 17 427 Cuts at: 3135 3664 4012 5467 618 Size: 529 348 1455 1842 Fragments arranged by size: 1842 1455 627 529 427 421 355 348 325 217 128 17 BanII G_rGCy'C Cuts at: 1391 2261 3360 3535 3854 4010 1391 Size: 870 1099 175 319 156 4072 Fragments arranged by size: 4072 1099 870 319 175 156 BbeI G_GCGC'C Cuts at: 2712 2712 Size: 6691 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2514 3366 928 Size: 971 615 852 4253 Fragments arranged by size: 4253 971 852 615 BbvCI CC'TCA_GC Cuts at: 2726 3108 2726 Size: 382 6309 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2806 2840 3673 3707 4882 4916 1903 Size: 34 869 34 833 34 1175 34 3678 Fragments arranged by size: 3678 1175 869 833 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4583 5793 4583 Size: 1210 5481 BclI T'GATC_A Cuts at: 1548 1548 Size: 6691 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5302 136 Size: 107 122 71 631 605 3630 1525 Fragments arranged by size: 3630 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6686 6686 Size: 6691 BlpI GC'TnA_GC Cuts at: 2649 4108 2649 Size: 1459 5232 Bme1580I G_kGCm'C Cuts at: 1790 2261 3535 3636 3854 4752 5908 1790 Size: 471 1274 101 218 898 1156 2573 Fragments arranged by size: 2573 1274 1156 898 471 218 101 BmgBI CAC'GTC Cuts at: 1318 3463 1318 Size: 2145 4546 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2043 3228 4422 Size: 416 53 83 399 24 626 1185 1194 Cuts at: 4422 5380 442 Size: 958 1753 Fragments arranged by size: 1753 1194 1185 958 626 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6691 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2751 2816 2828 2942 3321 3743 3752 5351 Size: 1512 65 12 114 379 422 9 1599 Cuts at: 5351 1239 Size: 2579 Fragments arranged by size: 2579 1599 1512 422 379 114 65 12 9 Bpu10I CC'TnA_GC Cuts at: 2321 2726 3108 3855 2321 Size: 405 382 747 5157 Fragments arranged by size: 5157 747 405 382 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2135 3275 4816 5681 5979 2135 Size: 1140 1541 865 298 2847 Fragments arranged by size: 2847 1541 1140 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2015 3232 5354 882 Size: 617 516 1217 2122 2219 Fragments arranged by size: 2219 2122 1217 617 516 BsaAI yAC'GTr Cuts at: 493 1204 3280 4482 493 Size: 711 2076 1202 2702 Fragments arranged by size: 2702 2076 1202 711 BsaBI GATnn'nnATC Cuts at: 3776 6685 3776 Size: 2909 3782 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2226 2316 2709 3799 Size: 53 83 186 936 693 90 393 1090 Cuts at: 3799 4498 4880 5515 275 Size: 699 382 635 1451 Fragments arranged by size: 1451 1090 936 699 693 635 393 382 186 90 83 53 BsaWI w'CCGG_w Cuts at: 2164 2421 2616 3921 5124 5522 5796 5943 6354 Size: 257 195 1305 1203 398 274 147 411 Cuts at: 6354 2164 Size: 2501 Fragments arranged by size: 2501 1305 1203 411 398 274 257 195 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2196 2226 3264 3294 1178 Size: 30 165 30 793 30 1038 30 4575 Fragments arranged by size: 4575 1038 793 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3848 3851 3902 1186 Size: 2662 3 51 3975 Fragments arranged by size: 3975 2662 51 3 BseYI C'CCAG_C Cuts at: 2298 2462 2790 3039 3790 3962 5894 6390 2298 Size: 164 328 249 751 172 1932 496 2599 Fragments arranged by size: 2599 1932 751 496 328 249 172 164 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2090 2273 3302 3595 3664 1163 Size: 573 354 183 1029 293 69 4190 Fragments arranged by size: 4190 1029 573 354 293 183 69 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2344 3352 4141 4902 5930 1540 Size: 275 186 343 1008 789 761 1028 2301 Fragments arranged by size: 2301 1028 1008 789 761 343 275 186 BsiHKAI G_wGCw'C Cuts at: 3263 3636 4752 4837 5908 3263 Size: 373 1116 85 1071 4046 Fragments arranged by size: 4046 1116 1071 373 85 BsmI GAATG_Cn' Cuts at: 3931 4202 4295 3931 Size: 271 93 6327 Fragments arranged by size: 6327 271 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2261 3263 3360 3535 3636 3854 4010 Size: 399 471 1002 97 175 101 218 156 Cuts at: 4010 4752 4837 5908 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 1002 742 471 399 218 175 156 101 97 85 BspEI T'CCGG_A Cuts at: 3921 6354 3921 Size: 2433 4258 BspHI T'CATG_A Cuts at: 4580 6310 4580 Size: 1730 4961 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3189 3617 3963 4046 844 Size: 2345 428 346 83 3489 Fragments arranged by size: 3489 2345 428 346 83 BsrBI CCG'CTC Cuts at: 1394 1823 4578 1394 Size: 429 2755 3507 Fragments arranged by size: 3507 2755 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5186 5360 65 Size: 1183 3938 174 1396 Fragments arranged by size: 3938 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2051 2339 2421 3614 5335 5522 1700 Size: 110 241 288 82 1193 1721 187 2869 Fragments arranged by size: 2869 1721 1193 288 241 187 110 82 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6691 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6691 BssSI C'ACGA_G Cuts at: 4751 5763 4751 Size: 1012 5679 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6691 BstBI TT'CG_AA Cuts at: 2826 5495 2826 Size: 2669 4022 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6691 BstXI CCAn_nnnn'nTGG Cuts at: 2548 2719 3021 3970 2548 Size: 171 302 949 5269 Fragments arranged by size: 5269 949 302 171 BstYI r'GATC_y Cuts at: 1972 2244 2599 2975 3007 3918 4021 4388 4773 Size: 272 355 376 32 911 103 367 385 Cuts at: 4773 4790 6242 6328 6340 6686 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 911 385 376 367 355 346 272 103 86 32 17 12 Bsu36I CC'TnA_GG Cuts at: 2364 3861 2364 Size: 1497 5194 BtgI C'CryG_G Cuts at: 513 1065 2004 2718 2943 513 Size: 552 939 714 225 4261 Fragments arranged by size: 4261 939 714 552 225 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5952 Fragments arranged by size: 5952 683 56 BtsI GCAGTG_nn' Cuts at: 3740 4207 5001 5021 3740 Size: 467 794 20 5410 Fragments arranged by size: 5410 794 467 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6691 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6656 DraI TTT'AAA Cuts at: 3995 3995 Size: 6691 DraIII CAC_nnn'GTG Cuts at: 3597 3597 Size: 6691 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5698 809 Size: 507 4382 1802 Fragments arranged by size: 4382 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2086 2341 2538 Size: 54 1425 50 275 18 256 255 197 Cuts at: 2538 2550 3030 3123 3345 3616 3984 4138 5027 Size: 12 480 93 222 271 368 154 889 Cuts at: 5027 6656 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 480 368 275 271 256 255 222 197 154 93 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2341 4138 1537 Size: 275 529 1797 4090 Fragments arranged by size: 4090 1797 529 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2388 2397 4621 1353 Size: 1035 9 2224 3423 Fragments arranged by size: 3423 2224 1035 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5278 5652 5798 387 Size: 1064 138 3689 374 146 1280 Fragments arranged by size: 3689 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2352 2751 2816 2828 2942 3321 3743 Size: 393 720 399 65 12 114 379 422 Cuts at: 3743 3752 3893 4754 5351 6138 6406 1239 Size: 9 141 861 597 787 268 1524 Fragments arranged by size: 1524 861 787 720 597 422 399 393 379 268 141 114 65 12 9 EcoNI CCTnn'n_nnAGG Cuts at: 2216 3905 2216 Size: 1689 5002 EcoO109I rG'GnC_Cy Cuts at: 2037 2257 2258 2359 2368 3531 3851 2037 Size: 220 1 101 9 1163 320 4877 Fragments arranged by size: 4877 1163 320 220 101 9 1 EcoRI G'AATT_C Cuts at: 4000 4378 4000 Size: 378 6313 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3394 3394 Size: 6691 FspI TGC'GCA Cuts at: 2201 3047 5197 2201 Size: 846 2150 3695 Fragments arranged by size: 3695 2150 846 HaeII r_GCGC'y Cuts at: 1678 2108 2712 2734 5838 1678 Size: 430 604 22 3104 2531 Fragments arranged by size: 3104 2531 604 430 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3062 3094 3484 3516 3641 Size: 32 135 32 1490 32 390 32 125 Cuts at: 3641 3673 5332 5364 5406 5438 1373 Size: 32 1659 32 42 32 2626 Fragments arranged by size: 2626 1659 1490 390 135 125 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2521 4035 4281 669 Size: 988 281 583 1514 246 3079 Fragments arranged by size: 3079 1514 988 583 281 246 HindIII A'AGCT_T Cuts at: 748 3164 748 Size: 2416 4275 HpaI GTT'AAC Cuts at: 4281 4281 Size: 6691 KasI G'GCGC_C Cuts at: 2708 2708 Size: 6691 KpnI G_GTAC'C Cuts at: 2695 4016 2695 Size: 1321 5370 MfeI C'AATT_G Cuts at: 4290 4290 Size: 6691 MluI A'CGCG_T Cuts at: 6567 6567 Size: 6691 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2082 5805 5989 1305 Size: 777 3723 184 2007 Fragments arranged by size: 3723 2007 777 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2540 3032 3125 3986 10 Size: 54 1425 343 708 492 93 861 2715 Fragments arranged by size: 2715 1425 861 708 492 343 93 54 MslI CAynn'nnrTG Cuts at: 518 1166 2024 3119 4649 5008 5167 518 Size: 648 858 1095 1530 359 159 2042 Fragments arranged by size: 2042 1530 1095 858 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2074 2182 2298 2794 2962 3286 3646 3790 Size: 468 108 116 496 168 324 360 144 Cuts at: 3790 4107 4784 5932 6177 1606 Size: 317 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 496 468 360 324 317 245 168 144 116 108 NarI GG'CG_CC Cuts at: 2709 2709 Size: 6691 NcoI C'CATG_G Cuts at: 513 1065 2004 2943 513 Size: 552 939 939 4261 Fragments arranged by size: 4261 939 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6691 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6691 NotI GC'GGCC_GC Cuts at: 4138 4138 Size: 6691 NspI r_CATG'y Cuts at: 1132 1229 3943 4049 5594 1132 Size: 97 2714 106 1545 2229 Fragments arranged by size: 2714 2229 1545 106 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6691 PciI A'CATG_T Cuts at: 5590 5590 Size: 6691 PflMI CCAn_nnn'nTGG Cuts at: 1108 3039 3783 1108 Size: 1931 744 4016 Fragments arranged by size: 4016 1931 744 PfoI T'CCnGG_A Cuts at: 2666 2666 Size: 6691 PmeI GTTT'AAAC Cuts at: 3995 3995 Size: 6691 PmlI CAC'GTG Cuts at: 3280 3280 Size: 6691 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2267 2299 4760 4792 6273 6305 2267 Size: 32 2461 32 1481 32 2653 Fragments arranged by size: 2653 2461 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2359 2359 Size: 6691 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6691 PsiI TTA'TAA Cuts at: 3170 4261 3170 Size: 1091 5600 PspOMI G'GGCC_C Cuts at: 2257 3531 3850 2257 Size: 1274 319 5098 Fragments arranged by size: 5098 1274 319 PstI C_TGCA'G Cuts at: 830 2787 3078 3492 4043 830 Size: 1957 291 414 551 3478 Fragments arranged by size: 3478 1957 551 414 291 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6691 PvuII CAG'CTG Cuts at: 2074 2794 2962 3286 3646 3790 2074 Size: 720 168 324 360 144 4975 Fragments arranged by size: 4975 720 360 324 168 144 SalI G'TCGA_C Cuts at: 1936 4033 1936 Size: 2097 4594 SapI GCTCTTCn'nnn_ Cuts at: 2388 2388 Size: 6691 SbfI CC_TGCA'GG Cuts at: 4043 4043 Size: 6691 ScaI AGT'ACT Cuts at: 4939 6651 4939 Size: 1712 4979 SexAI A'CCwGG_T Cuts at: 2064 3494 3573 2064 Size: 1430 79 5182 Fragments arranged by size: 5182 1430 79 SfcI C'TryA_G Cuts at: 826 1045 2783 2875 3074 3316 3488 4039 5174 Size: 219 1738 92 199 242 172 551 1135 Cuts at: 5174 5855 6046 826 Size: 681 191 1471 Fragments arranged by size: 1738 1471 1135 681 551 242 219 199 191 172 92 SfoI GGC'GCC Cuts at: 2710 2710 Size: 6691 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6691 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6691 SmaI CCC'GGG Cuts at: 1872 4018 1872 Size: 2146 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2150 2216 3290 4795 5696 5958 Size: 197 926 207 66 1074 1505 901 262 Cuts at: 5958 820 Size: 1553 Fragments arranged by size: 1553 1505 1074 926 901 262 207 197 66 SnaBI TAC'GTA Cuts at: 493 4482 493 Size: 3989 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6691 SphI G_CATG'C Cuts at: 1132 4049 1132 Size: 2917 3774 SspI AAT'ATT Cuts at: 5 52 4615 5 Size: 47 4563 2081 Fragments arranged by size: 4563 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6691 StyI C'CwwG_G Cuts at: 513 1065 2004 2541 2943 3708 4130 513 Size: 552 939 537 402 765 422 3074 Fragments arranged by size: 3074 939 765 552 537 422 402 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2290 3031 3555 3652 4718 4735 4888 Size: 1255 710 741 524 97 1066 17 153 Cuts at: 4888 5073 5518 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1066 741 710 524 445 185 153 97 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4937 6649 96 Size: 274 80 33 51 1443 2960 1712 138 Fragments arranged by size: 2960 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3264 3296 6651 6683 3264 Size: 32 3355 32 3272 Fragments arranged by size: 3355 3272 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2276 1316 Size: 960 5731 XbaI T'CTAG_A Cuts at: 2602 4027 2602 Size: 1425 5266 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2702 3220 2702 Size: 518 6173 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6691 XmaI C'CCGG_G Cuts at: 1870 4016 1870 Size: 2146 4545 XmnI GAAnn'nnTTC Cuts at: 3878 4820 3878 Size: 942 5749 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4499 276 Size: 53 83 186 936 2965 2468 Fragments arranged by size: 2965 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI