Restriction map for
pFN21AE2043
(Linear) (Six-base) MAPSORT of: KIEE2043.seq Check: 2342 from: 1 to: 1986
KIEE2043.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1184 1612 1986
Size: 1184 428 374
Fragments arranged by size:
1184 428 374
Acc65I G'GTAC_C
Cuts at: 0 686 1986
Size: 686 1300
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 1888 1986
Size: 347 1541 98
Fragments arranged by size:
1541 347 98
AflII C'TTAA_G
Cuts at: 0 211 1986
Size: 211 1775
AgeI A'CCGG_T
Cuts at: 0 416 1986
Size: 416 1570
AhdI GACnn_n'nnGTC
Cuts at: 0 1493 1986
Size: 1493 493
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 905 1986
Size: 905 1081
AlwNI CAG_nnn'CTG
Cuts at: 0 366 885 1986
Size: 366 519 1101
Fragments arranged by size:
1101 519 366
ApaI G_GGCC'C
Cuts at: 0 256 1530 1849 1986
Size: 256 1274 319 137
Fragments arranged by size:
1274 319 256 137
ApaLI G'TGCA_C
Cuts at: 0 1627 1986
Size: 1627 359
ApoI r'AATT_y
Cuts at: 0 1235 1986
Size: 1235 751
AvaI C'yCGr_G
Cuts at: 0 103 1562 1986
Size: 103 1459 424
Fragments arranged by size:
1459 424 103
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 148 181 1986
Size: 148 33 1805
Fragments arranged by size:
1805 148 33
BamHI G'GATC_C
Cuts at: 0 970 1002 1986
Size: 970 32 984
Fragments arranged by size:
984 970 32
BanI G'GyrC_C
Cuts at: 0 203 331 686 703 1130 1659 1986
Size: 203 128 355 17 427 529 327
Fragments arranged by size:
529 427 355 327 203 128 17
BanII G_rGCy'C
Cuts at: 0 256 1355 1530 1849 1986
Size: 256 1099 175 319 137
Fragments arranged by size:
1099 319 256 175 137
BbeI G_GCGC'C
Cuts at: 0 707 1986
Size: 707 1279
BbsI GAAGACnn'nnnn_
Cuts at: 0 509 1361 1986
Size: 509 852 625
Fragments arranged by size:
852 625 509
BbvCI CC'TCA_GC
Cuts at: 0 721 1103 1986
Size: 721 382 883
Fragments arranged by size:
883 721 382
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 801 835 1668 1702 1986
Size: 801 34 833 34 284
Fragments arranged by size:
833 801 284 34 34
BlpI GC'TnA_GC
Cuts at: 0 644 1986
Size: 644 1342
Bme1580I G_kGCm'C
Cuts at: 0 256 1530 1631 1849 1986
Size: 256 1274 101 218 137
Fragments arranged by size:
1274 256 218 137 101
BmgBI CAC'GTC
Cuts at: 0 1458 1986
Size: 1458 528
BmrI ACTGGGnnnn_n'
Cuts at: 0 38 1223 1986
Size: 38 1185 763
Fragments arranged by size:
1185 763 38
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 811 823 937 1316 1738 1747 1986
Size: 746 65 12 114 379 422 9 239
Fragments arranged by size:
746 422 379 239 114 65 12 9
Bpu10I CC'TnA_GC
Cuts at: 0 316 721 1103 1850 1986
Size: 316 405 382 747 136
Fragments arranged by size:
747 405 382 316 136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 1270 1986
Size: 130 1140 716
Fragments arranged by size:
1140 716 130
BsaI GGTCTCn'nnnn_
Cuts at: 0 10 1227 1986
Size: 10 1217 759
Fragments arranged by size:
1217 759 10
BsaAI yAC'GTr
Cuts at: 0 1275 1986
Size: 1275 711
BsaBI GATnn'nnATC
Cuts at: 0 1771 1986
Size: 1771 215
BsaHI Gr'CG_yC
Cuts at: 0 221 311 704 1794 1986
Size: 221 90 393 1090 192
Fragments arranged by size:
1090 393 221 192 90
BsaWI w'CCGG_w
Cuts at: 0 159 416 611 1916 1986
Size: 159 257 195 1305 70
Fragments arranged by size:
1305 257 195 159 70
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 191 221 1259 1289 1986
Size: 191 30 1038 30 697
Fragments arranged by size:
1038 697 191 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1843 1846 1897 1986
Size: 1843 3 51 89
Fragments arranged by size:
1843 89 51 3
BseYI C'CCAG_C
Cuts at: 0 293 457 785 1034 1785 1957 1986
Size: 293 164 328 249 751 172 29
Fragments arranged by size:
751 328 293 249 172 164 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 268 1297 1590 1659 1986
Size: 85 183 1029 293 69 327
Fragments arranged by size:
1029 327 293 183 85 69
BsiEI CG_ry'CG
Cuts at: 0 339 1347 1986
Size: 339 1008 639
Fragments arranged by size:
1008 639 339
BsiHKAI G_wGCw'C
Cuts at: 0 1258 1631 1986
Size: 1258 373 355
Fragments arranged by size:
1258 373 355
BsmI GAATG_Cn'
Cuts at: 0 1926 1986
Size: 1926 60
Bsp1286I G_dGCh'C
Cuts at: 0 256 1258 1355 1530 1631 1849 1986
Size: 256 1002 97 175 101 218 137
Fragments arranged by size:
1002 256 218 175 137 101 97
BspEI T'CCGG_A
Cuts at: 0 1916 1986
Size: 1916 70
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1184 1612 1958 1986
Size: 1184 428 346 28
Fragments arranged by size:
1184 428 346 28
BsrFI r'CCGG_y
Cuts at: 0 46 334 416 1609 1986
Size: 46 288 82 1193 377
Fragments arranged by size:
1193 377 288 82 46
BstBI TT'CG_AA
Cuts at: 0 821 1986
Size: 821 1165
BstXI CCAn_nnnn'nTGG
Cuts at: 0 543 714 1016 1965 1986
Size: 543 171 302 949 21
Fragments arranged by size:
949 543 302 171 21
BstYI r'GATC_y
Cuts at: 0 239 594 970 1002 1913 1986
Size: 239 355 376 32 911 73
Fragments arranged by size:
911 376 355 239 73 32
Bsu36I CC'TnA_GG
Cuts at: 0 359 1856 1986
Size: 359 1497 130
Fragments arranged by size:
1497 359 130
BtgI C'CryG_G
Cuts at: 0 713 938 1986
Size: 713 225 1048
Fragments arranged by size:
1048 713 225
BtsI GCAGTG_nn'
Cuts at: 0 1735 1986
Size: 1735 251
DraIII CAC_nnn'GTG
Cuts at: 0 1592 1986
Size: 1592 394
EaeI y'GGCC_r
Cuts at: 0 81 336 533 545 1025 1118 1340 1611
Size: 81 255 197 12 480 93 222 271
Cuts at: 1611 1979 1986
Size: 368 7
Fragments arranged by size:
480 368 271 255 222 197 93 81
12 7
EagI C'GGCC_G
Cuts at: 0 336 1986
Size: 336 1650
EarI CTCTTCn'nnn_
Cuts at: 0 383 392 1986
Size: 383 9 1594
Fragments arranged by size:
1594 383 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 746 811 823 937 1316 1738 1747
Size: 347 399 65 12 114 379 422 9
Cuts at: 1747 1888 1986
Size: 141 98
Fragments arranged by size:
422 399 379 347 141 114 98 65
12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 211 1900 1986
Size: 211 1689 86
Fragments arranged by size:
1689 211 86
EcoO109I rG'GnC_Cy
Cuts at: 0 32 252 253 354 363 1526 1846 1986
Size: 32 220 1 101 9 1163 320 140
Fragments arranged by size:
1163 320 220 140 101 32 9 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1389 1986
Size: 1389 597
FspI TGC'GCA
Cuts at: 0 196 1042 1986
Size: 196 846 944
Fragments arranged by size:
944 846 196
HaeII r_GCGC'y
Cuts at: 0 103 707 729 1986
Size: 103 604 22 1257
Fragments arranged by size:
1257 604 103 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1057 1089 1479 1511 1636 1668 1986
Size: 1057 32 390 32 125 32 318
Fragments arranged by size:
1057 390 318 125 32 32 32
HincII GTy'rAC
Cuts at: 0 516 1986
Size: 516 1470
HindIII A'AGCT_T
Cuts at: 0 1159 1986
Size: 1159 827
KasI G'GCGC_C
Cuts at: 0 703 1986
Size: 703 1283
KpnI G_GTAC'C
Cuts at: 0 690 1986
Size: 690 1296
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 1986
Size: 77 1909
MscI TGG'CCA
Cuts at: 0 535 1027 1120 1981 1986
Size: 535 492 93 861 5
Fragments arranged by size:
861 535 492 93 5
MslI CAynn'nnrTG
Cuts at: 0 19 1114 1986
Size: 19 1095 872
Fragments arranged by size:
1095 872 19
MspA1I CmG'CkG
Cuts at: 0 69 177 293 789 957 1281 1641 1785
Size: 69 108 116 496 168 324 360 144
Cuts at: 1785 1986
Size: 201
Fragments arranged by size:
496 360 324 201 168 144 116 108
69
NarI GG'CG_CC
Cuts at: 0 704 1986
Size: 704 1282
NcoI C'CATG_G
Cuts at: 0 938 1986
Size: 938 1048
NspI r_CATG'y
Cuts at: 0 1938 1986
Size: 1938 48
PflMI CCAn_nnn'nTGG
Cuts at: 0 1034 1778 1986
Size: 1034 744 208
Fragments arranged by size:
1034 744 208
PfoI T'CCnGG_A
Cuts at: 0 661 1986
Size: 661 1325
PmlI CAC'GTG
Cuts at: 0 1275 1986
Size: 1275 711
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 262 294 1986
Size: 262 32 1692
Fragments arranged by size:
1692 262 32
PpuMI rG'GwC_Cy
Cuts at: 0 354 1986
Size: 354 1632
PsiI TTA'TAA
Cuts at: 0 1165 1986
Size: 1165 821
PspOMI G'GGCC_C
Cuts at: 0 252 1526 1845 1986
Size: 252 1274 319 141
Fragments arranged by size:
1274 319 252 141
PstI C_TGCA'G
Cuts at: 0 782 1073 1487 1986
Size: 782 291 414 499
Fragments arranged by size:
782 499 414 291
PvuII CAG'CTG
Cuts at: 0 69 789 957 1281 1641 1785 1986
Size: 69 720 168 324 360 144 201
Fragments arranged by size:
720 360 324 201 168 144 69
SapI GCTCTTCn'nnn_
Cuts at: 0 383 1986
Size: 383 1603
SexAI A'CCwGG_T
Cuts at: 0 59 1489 1568 1986
Size: 59 1430 79 418
Fragments arranged by size:
1430 418 79 59
SfcI C'TryA_G
Cuts at: 0 778 870 1069 1311 1483 1986
Size: 778 92 199 242 172 503
Fragments arranged by size:
778 503 242 199 172 92
SfoI GGC'GCC
Cuts at: 0 705 1986
Size: 705 1281
SmlI C'TyrA_G
Cuts at: 0 145 211 1285 1986
Size: 145 66 1074 701
Fragments arranged by size:
1074 701 145 66
StyI C'CwwG_G
Cuts at: 0 536 938 1703 1986
Size: 536 402 765 283
Fragments arranged by size:
765 536 402 283
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 285 1026 1550 1647 1986
Size: 285 741 524 97 339
Fragments arranged by size:
741 524 339 285 97
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1259 1291 1986
Size: 1259 32 695
Fragments arranged by size:
1259 695 32
Tth111I GACn'n_nGTC
Cuts at: 0 271 1986
Size: 271 1715
XbaI T'CTAG_A
Cuts at: 0 597 1986
Size: 597 1389
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 697 1215 1986
Size: 697 518 771
Fragments arranged by size:
771 697 518
XmnI GAAnn'nnTTC
Cuts at: 0 1873 1986
Size: 1873 113
Enzymes that do cut:
AarI Acc65I AcuI AflII AgeI AhdI AlfI AlwNI
ApaI ApaLI ApoI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrFI BstBI BstXI BstYI Bsu36I BtgI
BtsI DraIII EaeI EagI EarI Eco57MI EcoNI EcoO109I
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MmeI MscI MslI MspA1I NarI NcoI NspI PflMI
PfoI PmlI PpiI PpuMI PsiI PspOMI PstI PvuII
SapI SexAI SfcI SfoI SmlI StyI TaqII TstI
Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflIII AleI AloI AscI
AseI AsiSI AvrII BciVI BclI BfrBI BglI BglII
BmtI BplI BsiWI BsmBI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstEII BstZ17I BtgZI ClaI CspCI
DraI DrdI EciI EcoICRI EcoRI EcoRV FseI FspAI
HpaI MfeI MluI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PshAI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI XhoI XmaI ZraI