(Circular) (Six-base) MAPSORT of: pFN21AB9999.seq Check: 2289 from: 1 to: 8701 pFN21AB9999 8701 bp With 182 enzymes: SgfI * November 11, 2011 14:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3415 5602 3415 Size: 2187 6514 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6511 278 Size: 53 83 186 936 4975 2468 Fragments arranged by size: 4975 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6044 1937 Size: 4107 4594 Acc65I G'GTAC_C Cuts at: 6022 6022 Size: 8701 AclI AA'CG_TT Cuts at: 1563 5274 6828 7201 1563 Size: 3711 1554 373 3063 Fragments arranged by size: 3711 3063 1554 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2246 2777 2906 3262 3541 3764 4172 5266 Size: 614 531 129 356 279 223 408 1094 Cuts at: 5266 5924 6764 8148 8416 1632 Size: 658 840 1384 268 1917 Fragments arranged by size: 1917 1384 1094 840 658 614 531 408 356 279 268 223 129 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8504 AflIII A'CryG_T Cuts at: 1203 3012 7600 8577 1203 Size: 1809 4588 977 1327 Fragments arranged by size: 4588 1809 1327 977 AgeI A'CCGG_T Cuts at: 7532 7532 Size: 8701 AhdI GACnn_n'nnGTC Cuts at: 2249 7430 2249 Size: 5181 3520 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8701 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2354 1184 Size: 1170 7531 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2828 2860 2828 Size: 32 8669 AlwNI CAG_nnn'CTG Cuts at: 2500 3742 4408 5038 5251 5289 5708 5971 6444 Size: 1242 666 630 213 38 419 263 473 Cuts at: 6444 8016 2500 Size: 1572 3185 Fragments arranged by size: 3185 1572 1242 666 630 473 419 263 213 38 ApaI G_GGCC'C Cuts at: 4702 4892 5072 4702 Size: 190 180 8331 Fragments arranged by size: 8331 190 180 ApaLI G'TGCA_C Cuts at: 3016 6758 7914 3016 Size: 3742 1156 3803 Fragments arranged by size: 3803 3742 1156 ApoI r'AATT_y Cuts at: 1493 6010 6236 6388 8497 1493 Size: 4517 226 152 2109 1697 Fragments arranged by size: 4517 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7255 160 Size: 872 6223 1606 Fragments arranged by size: 6223 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8701 AvaI C'yCGr_G Cuts at: 1870 1943 2246 3531 4088 4523 4929 5153 5798 Size: 73 303 1285 557 435 406 224 645 Cuts at: 5798 6026 1870 Size: 228 4545 Fragments arranged by size: 4545 1285 645 557 435 406 303 228 224 73 BamHI G'GATC_C Cuts at: 2017 2705 6031 6398 2017 Size: 688 3326 367 4320 Fragments arranged by size: 4320 3326 688 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2060 2677 3074 3090 3595 Size: 325 217 627 273 617 397 16 505 Cuts at: 3595 5016 5711 6022 7477 618 Size: 1421 695 311 1455 1842 Fragments arranged by size: 1842 1455 1421 695 627 617 505 397 325 311 273 217 16 BanII G_rGCy'C Cuts at: 1391 2655 3203 4702 4892 4955 5072 5495 5729 Size: 1264 548 1499 190 63 117 423 234 Cuts at: 5729 5797 6020 1391 Size: 68 223 4072 Fragments arranged by size: 4072 1499 1264 548 423 234 223 190 117 68 63 BbeI G_GCGC'C Cuts at: 2064 5715 2064 Size: 3651 5050 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2322 2844 5117 928 Size: 971 423 522 2273 4512 Fragments arranged by size: 4512 2273 971 522 423 BbvCI CC'TCA_GC Cuts at: 3347 3575 4821 5000 3347 Size: 228 1246 179 7048 Fragments arranged by size: 7048 1246 228 179 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6892 6926 1903 Size: 34 4955 34 3678 Fragments arranged by size: 4955 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4424 6593 7803 4424 Size: 2169 1210 5322 Fragments arranged by size: 5322 2169 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8701 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2931 3536 3980 Size: 107 122 71 631 605 1259 605 444 Cuts at: 3980 4364 7312 136 Size: 384 2948 1525 Fragments arranged by size: 2948 1525 1259 631 605 605 444 384 122 107 71 BglII A'GATC_T Cuts at: 3963 8696 3963 Size: 4733 3968 BlpI GC'TnA_GC Cuts at: 3024 4562 6118 3024 Size: 1538 1556 5607 Fragments arranged by size: 5607 1556 1538 Bme1580I G_kGCm'C Cuts at: 1790 2682 3020 3077 4060 4663 4702 4892 5072 Size: 892 338 57 983 603 39 190 180 Cuts at: 5072 5374 6762 7918 1790 Size: 302 1388 1156 2573 Fragments arranged by size: 2573 1388 1156 983 892 603 338 302 190 180 57 39 BmgBI CAC'GTC Cuts at: 1318 2989 4258 1318 Size: 1671 1269 5761 Fragments arranged by size: 5761 1671 1269 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2500 5472 6432 Size: 416 53 83 399 24 1083 2972 960 Cuts at: 6432 7390 442 Size: 958 1753 Fragments arranged by size: 2972 1753 1083 960 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8701 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2415 3778 2415 Size: 1363 7338 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2061 2198 3233 3419 3773 4562 4571 5090 Size: 822 137 1035 186 354 789 9 519 Cuts at: 5090 5282 5371 7361 1239 Size: 192 89 1990 2579 Fragments arranged by size: 2579 1990 1035 822 789 519 354 192 186 137 89 9 Bpu10I CC'TnA_GC Cuts at: 2139 3105 3197 3327 3347 3575 4821 5000 5658 Size: 966 92 130 20 228 1246 179 658 Cuts at: 5658 2139 Size: 5182 Fragments arranged by size: 5182 1246 966 658 228 179 130 92 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2675 2816 3809 6826 7691 7989 2675 Size: 141 993 3017 865 298 3387 Fragments arranged by size: 3387 3017 993 865 298 141 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2386 2572 4085 7364 882 Size: 617 887 186 1513 3279 2219 Fragments arranged by size: 3279 2219 1513 887 617 186 BsaAI yAC'GTr Cuts at: 493 1204 3013 3070 6492 493 Size: 711 1809 57 3422 2702 Fragments arranged by size: 3422 2702 1809 711 57 BsaBI GATnn'nnATC Cuts at: 8695 8695 Size: 8701 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2061 5684 5712 6508 Size: 53 83 186 936 528 3623 28 796 Cuts at: 6508 6890 7525 275 Size: 382 635 1451 Fragments arranged by size: 3623 1451 936 796 635 528 382 186 83 53 28 BsaWI w'CCGG_w Cuts at: 3726 7134 7532 7806 7953 8364 3726 Size: 3408 398 274 147 411 4063 Fragments arranged by size: 4063 3408 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2543 2573 2999 3029 3248 Size: 30 165 30 1140 30 426 30 219 Cuts at: 3248 3278 3491 3521 4022 4052 5120 5150 1178 Size: 30 213 30 501 30 1068 30 4729 Fragments arranged by size: 4729 1140 1068 501 426 219 213 165 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2761 3422 3794 3812 4226 4241 4250 4253 Size: 1575 661 372 18 414 15 9 3 Cuts at: 4253 4256 4352 4355 1186 Size: 3 96 3 5532 Fragments arranged by size: 5532 1575 661 414 372 96 18 15 9 3 3 3 BseYI C'CCAG_C Cuts at: 2025 2667 2929 3853 3984 3989 4301 4312 4345 Size: 642 262 924 131 5 312 11 33 Cuts at: 4345 4871 5212 5621 5665 5729 5930 7904 8400 Size: 526 341 409 44 64 201 1974 496 Cuts at: 8400 2025 Size: 2326 Fragments arranged by size: 2326 1974 924 642 526 496 409 341 312 262 201 131 64 44 33 11 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2350 2951 3377 3662 4179 4604 4688 Size: 573 614 601 426 285 517 425 84 Cuts at: 4688 4748 5381 1163 Size: 60 633 4483 Fragments arranged by size: 4483 633 614 601 573 517 426 425 285 84 60 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2077 6151 6912 7940 1540 Size: 275 186 76 4074 761 1028 2301 Fragments arranged by size: 4074 2301 1028 761 275 186 76 BsiHKAI G_wGCw'C Cuts at: 2427 2655 3012 3020 4955 5028 5328 5797 6762 Size: 228 357 8 1935 73 300 469 965 Cuts at: 6762 6847 7918 2427 Size: 85 1071 3210 Fragments arranged by size: 3210 1935 1071 965 469 357 300 228 85 73 8 BsmI GAATG_Cn' Cuts at: 4534 6212 6305 4534 Size: 1678 93 6930 Fragments arranged by size: 6930 1678 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2141 2979 4422 5528 2141 Size: 838 1443 1106 5314 Fragments arranged by size: 5314 1443 1106 838 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2427 2655 2682 3012 3020 3077 3203 Size: 399 637 228 27 330 8 57 126 Cuts at: 3203 4060 4663 4702 4892 4955 5028 5072 5328 Size: 857 603 39 190 63 73 44 256 Cuts at: 5328 5374 5495 5729 5797 6020 6762 6847 7918 Size: 46 121 234 68 223 742 85 1071 Cuts at: 7918 1391 Size: 2174 Fragments arranged by size: 2174 1071 857 742 637 603 399 330 256 234 228 223 190 126 121 85 73 68 63 57 46 44 39 27 8 BspEI T'CCGG_A Cuts at: 8364 8364 Size: 8701 BspHI T'CATG_A Cuts at: 6590 8320 6590 Size: 1730 6971 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2373 2404 2823 3415 3858 3878 4800 5297 Size: 1529 31 419 592 443 20 922 497 Cuts at: 5297 5602 5824 6056 844 Size: 305 222 232 3489 Fragments arranged by size: 3489 1529 922 592 497 443 419 305 232 222 31 20 BsrBI CCG'CTC Cuts at: 1394 1823 3109 4144 4720 6588 1394 Size: 429 1286 1035 576 1868 3507 Fragments arranged by size: 3507 1868 1286 1035 576 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2127 2391 2579 2802 7196 7370 65 Size: 1183 879 264 188 223 4394 174 1396 Fragments arranged by size: 4394 1396 1183 879 264 223 188 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5381 5603 7345 7532 1700 Size: 110 3571 222 1742 187 2869 Fragments arranged by size: 3571 2869 1742 222 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8701 BssHII G'CGCG_C Cuts at: 1925 4631 4690 1925 Size: 2706 59 5936 Fragments arranged by size: 5936 2706 59 BssSI C'ACGA_G Cuts at: 3806 6761 7773 3806 Size: 2955 1012 4734 Fragments arranged by size: 4734 2955 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2677 3799 4408 5023 794 Size: 1883 1122 609 615 4472 Fragments arranged by size: 4472 1883 1122 615 609 BstBI TT'CG_AA Cuts at: 7505 7505 Size: 8701 BstEII G'GTnAC_C Cuts at: 1184 4924 5191 1184 Size: 3740 267 4694 Fragments arranged by size: 4694 3740 267 BstXI CCAn_nnnn'nTGG Cuts at: 2809 2856 4909 5789 2809 Size: 47 2053 880 5721 Fragments arranged by size: 5721 2053 880 47 BstYI r'GATC_y Cuts at: 1972 2017 2589 2705 3483 3963 4479 5472 6031 Size: 45 572 116 778 480 516 993 559 Cuts at: 6031 6398 6783 6800 8252 8338 8350 8696 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 993 778 572 559 516 480 385 367 346 116 86 45 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2163 2849 3941 4884 5808 513 Size: 552 939 159 686 1092 943 924 3406 Fragments arranged by size: 3406 1092 943 939 924 686 552 159 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3297 487 Size: 683 56 2071 5891 Fragments arranged by size: 5891 2071 683 56 BtsI GCAGTG_nn' Cuts at: 3018 3553 3699 4815 6217 7011 7031 3018 Size: 535 146 1116 1402 794 20 4688 Fragments arranged by size: 4688 1402 1116 794 535 146 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8701 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8666 DraI TTT'AAA Cuts at: 6005 6005 Size: 8701 DraIII CAC_nnn'GTG Cuts at: 2974 3640 3679 5023 2974 Size: 666 39 1344 6652 Fragments arranged by size: 6652 1344 666 39 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7708 809 Size: 507 6392 1802 Fragments arranged by size: 6392 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2160 2763 2900 Size: 54 1425 50 275 18 330 603 137 Cuts at: 2900 3117 3429 4027 4738 6148 7037 8666 8 Size: 217 312 598 711 1410 889 1629 43 Fragments arranged by size: 1629 1425 1410 889 711 603 598 330 312 275 217 137 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6148 1537 Size: 275 4336 4090 Fragments arranged by size: 4336 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2660 4203 5012 5285 5633 5850 6631 1353 Size: 1307 1543 809 273 348 217 781 3423 Fragments arranged by size: 3423 1543 1307 809 781 348 273 217 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2810 2912 2984 7288 7662 7808 Size: 1064 138 1221 102 72 4304 374 146 Cuts at: 7808 387 Size: 1280 Fragments arranged by size: 4304 1280 1221 1064 374 146 138 102 72 EcoICRI GAG'CTC Cuts at: 2653 4953 5795 2653 Size: 2300 842 5559 Fragments arranged by size: 5559 2300 842 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2061 2198 2246 2777 2906 3233 3262 Size: 393 429 137 48 531 129 327 29 Cuts at: 3262 3419 3541 3764 3773 4172 4562 4571 5090 Size: 157 122 223 9 399 390 9 519 Cuts at: 5090 5266 5282 5371 5924 6764 7361 8148 8416 Size: 176 16 89 553 840 597 787 268 Cuts at: 8416 1239 Size: 1524 Fragments arranged by size: 1524 840 787 597 553 531 519 429 399 393 390 327 268 223 176 157 137 129 122 89 48 29 16 9 9 EcoNI CCTnn'n_nnAGG Cuts at: 2369 2561 2597 3874 4499 5186 5363 2369 Size: 192 36 1277 625 687 177 5707 Fragments arranged by size: 5707 1277 687 625 192 177 36 EcoO109I rG'GnC_Cy Cuts at: 2497 2542 2602 4612 4698 4699 5149 5179 5286 Size: 45 60 2010 86 1 450 30 107 Cuts at: 5286 5662 5802 2497 Size: 376 140 5396 Fragments arranged by size: 5396 2010 450 376 140 107 86 60 45 30 1 EcoRI G'AATT_C Cuts at: 6010 6388 6010 Size: 378 8323 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5326 5542 5878 5928 5326 Size: 216 336 50 8099 Fragments arranged by size: 8099 336 216 50 FspI TGC'GCA Cuts at: 3705 4586 7207 3705 Size: 881 2621 5199 Fragments arranged by size: 5199 2621 881 HaeII r_GCGC'y Cuts at: 1678 2064 2831 3715 4313 4669 5443 5715 7848 Size: 386 767 884 598 356 774 272 2133 Cuts at: 7848 1678 Size: 2531 Fragments arranged by size: 2531 2133 884 774 767 598 386 356 272 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2002 2034 2235 2267 2385 Size: 32 135 32 430 32 201 32 118 Cuts at: 2385 2417 2543 2575 2976 3008 3248 3280 3525 Size: 32 126 32 401 32 240 32 245 Cuts at: 3525 3557 5120 5152 5967 5999 7342 7374 7416 Size: 32 1563 32 815 32 1343 32 42 Cuts at: 7416 7448 1373 Size: 32 2626 Fragments arranged by size: 2626 1563 1343 815 430 401 245 240 201 135 126 118 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3274 6045 6291 669 Size: 988 281 1336 2771 246 3079 Fragments arranged by size: 3079 2771 1336 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8701 HpaI GTT'AAC Cuts at: 6291 6291 Size: 8701 KasI G'GCGC_C Cuts at: 2060 5711 2060 Size: 3651 5050 KpnI G_GTAC'C Cuts at: 6026 6026 Size: 8701 MfeI C'AATT_G Cuts at: 6300 6300 Size: 8701 MluI A'CGCG_T Cuts at: 8577 8577 Size: 8701 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5430 7815 7999 1305 Size: 4125 2385 184 2007 Fragments arranged by size: 4125 2385 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2902 3431 4029 10 Size: 54 1425 343 1070 529 598 4682 Fragments arranged by size: 4682 1425 1070 598 529 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 6659 7018 7177 518 Size: 648 5493 359 159 2042 Fragments arranged by size: 5493 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2299 2965 3115 4282 4558 4905 5167 5579 Size: 693 666 150 1167 276 347 262 412 Cuts at: 5579 6117 6794 7942 8187 1606 Size: 538 677 1148 245 2120 Fragments arranged by size: 2120 1167 1148 693 677 666 538 412 347 276 262 245 150 NarI GG'CG_CC Cuts at: 2061 5712 2061 Size: 3651 5050 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 7210 Fragments arranged by size: 7210 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8701 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8701 NotI GC'GGCC_GC Cuts at: 6148 6148 Size: 8701 NruI TCG'CGA Cuts at: 2103 2103 Size: 8701 NspI r_CATG'y Cuts at: 1132 1229 3325 4882 6059 7604 1132 Size: 97 2096 1557 1177 1545 2229 Fragments arranged by size: 2229 2096 1557 1545 1177 97 PasI CC'CwG_GG Cuts at: 1348 3610 5428 1348 Size: 2262 1818 4621 Fragments arranged by size: 4621 2262 1818 PciI A'CATG_T Cuts at: 7600 7600 Size: 8701 PflMI CCAn_nnn'nTGG Cuts at: 1108 4681 5708 1108 Size: 3573 1027 4101 Fragments arranged by size: 4101 3573 1027 PfoI T'CCnGG_A Cuts at: 4272 4539 4272 Size: 267 8434 PmeI GTTT'AAAC Cuts at: 6005 6005 Size: 8701 PmlI CAC'GTG Cuts at: 3013 3013 Size: 8701 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2705 2737 6770 6802 8283 8315 2705 Size: 32 4033 32 1481 32 3091 Fragments arranged by size: 4033 3091 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2602 4612 5149 5179 5286 5662 5802 2602 Size: 2010 537 30 107 376 140 5501 Fragments arranged by size: 5501 2010 537 376 140 107 30 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8701 PsiI TTA'TAA Cuts at: 6271 6271 Size: 8701 PspOMI G'GGCC_C Cuts at: 4698 4888 5068 4698 Size: 190 180 8331 Fragments arranged by size: 8331 190 180 PstI C_TGCA'G Cuts at: 830 2343 2496 3216 3738 3838 4065 4299 4449 Size: 1513 153 720 522 100 227 234 150 Cuts at: 4449 5343 5599 5760 5821 6053 830 Size: 894 256 161 61 232 3478 Fragments arranged by size: 3478 1513 894 720 522 256 234 232 227 161 153 150 100 61 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8701 PvuII CAG'CTG Cuts at: 2299 2965 3115 4282 4905 5167 2299 Size: 666 150 1167 623 262 5833 Fragments arranged by size: 5833 1167 666 623 262 150 SacI G_AGCT'C Cuts at: 2655 4955 5797 2655 Size: 2300 842 5559 Fragments arranged by size: 5559 2300 842 SalI G'TCGA_C Cuts at: 1936 6043 1936 Size: 4107 4594 SanDI GG'GwC_CC Cuts at: 5802 5802 Size: 8701 SapI GCTCTTCn'nnn_ Cuts at: 2660 5850 2660 Size: 3190 5511 SbfI CC_TGCA'GG Cuts at: 3216 5821 6053 3216 Size: 2605 232 5864 Fragments arranged by size: 5864 2605 232 ScaI AGT'ACT Cuts at: 6949 8661 6949 Size: 1712 6989 SexAI A'CCwGG_T Cuts at: 2970 3636 4494 4920 2970 Size: 666 858 426 6751 Fragments arranged by size: 6751 858 666 426 SfcI C'TryA_G Cuts at: 826 1045 2278 2339 2492 3212 3734 3834 4061 Size: 219 1233 61 153 720 522 100 227 Cuts at: 4061 4295 4445 5339 5360 5595 5756 5817 6049 Size: 234 150 894 21 235 161 61 232 Cuts at: 6049 7184 7865 8056 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1233 1135 894 720 681 522 235 234 232 227 219 191 161 153 150 100 61 61 21 SfiI GGCCn_nnn'nGGCC Cuts at: 3980 4364 3980 Size: 384 8317 SfoI GGC'GCC Cuts at: 2062 5713 2062 Size: 3651 5050 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8701 SgrAI Cr'CCGG_yG Cuts at: 1700 5603 1700 Size: 3903 4798 SmaI CCC'GGG Cuts at: 1872 4931 5800 6028 1872 Size: 3059 869 228 4545 Fragments arranged by size: 4545 3059 869 228 SmlI C'TyrA_G Cuts at: 820 1017 1943 2690 2831 3824 6805 7706 7968 Size: 197 926 747 141 993 2981 901 262 Cuts at: 7968 820 Size: 1553 Fragments arranged by size: 2981 1553 993 926 901 747 262 197 141 SnaBI TAC'GTA Cuts at: 493 6492 493 Size: 5999 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8701 SphI G_CATG'C Cuts at: 1132 6059 1132 Size: 4927 3774 SspI AAT'ATT Cuts at: 5 52 6625 5 Size: 47 6573 2081 Fragments arranged by size: 6573 2081 47 StuI AGG'CCT Cuts at: 1517 3156 3413 3719 5093 1517 Size: 1639 257 306 1374 5125 Fragments arranged by size: 5125 1639 1374 306 257 StyI C'CwwG_G Cuts at: 513 1065 2004 2955 3151 3267 5229 5310 5466 Size: 552 939 951 196 116 1962 81 156 Cuts at: 5466 6140 513 Size: 674 3074 Fragments arranged by size: 3074 1962 951 939 674 552 196 156 116 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3845 4304 4673 5321 5501 6728 6745 Size: 1255 2265 459 369 648 180 1227 17 Cuts at: 6745 6898 7083 7528 325 Size: 153 185 445 1498 Fragments arranged by size: 2265 1498 1255 1227 648 459 445 369 185 180 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4935 6947 8659 Size: 274 80 33 51 1443 2958 2012 1712 Cuts at: 8659 96 Size: 138 Fragments arranged by size: 2958 2012 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2999 3031 3491 3523 3926 3958 4022 4054 5141 Size: 32 460 32 403 32 64 32 1087 Cuts at: 5141 5173 5683 5715 8661 8693 2999 Size: 32 510 32 2946 32 3007 Fragments arranged by size: 3007 2946 1087 510 460 403 64 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5116 1316 Size: 3800 4901 XbaI T'CTAG_A Cuts at: 6037 6037 Size: 8701 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3611 4366 5425 3611 Size: 755 1059 6887 Fragments arranged by size: 6887 1059 755 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8701 XmaI C'CCGG_G Cuts at: 1870 4929 5798 6026 1870 Size: 3059 869 228 4545 Fragments arranged by size: 4545 3059 869 228 XmnI GAAnn'nnTTC Cuts at: 2717 6830 2717 Size: 4113 4588 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6509 276 Size: 53 83 186 936 4975 2468 Fragments arranged by size: 4975 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV NsiI PacI PspXI PsrI RsrII SacII SrfI SwaI