Restriction map for
pFN21AB9999
(Linear) (Six-base) MAPSORT of: KIBB9999.seq Check: 1224 from: 1 to: 3996
KIBB9999.orf
With 182 enzymes: SgfI *
November 11, 2011 11:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1410 3597 3996
Size: 1410 2187 399
Fragments arranged by size:
2187 1410 399
AclI AA'CG_TT
Cuts at: 0 3269 3996
Size: 3269 727
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 772 901 1257 1536 1759 2167 3261
Size: 241 531 129 356 279 223 408 1094
Cuts at: 3261 3919 3996
Size: 658 77
Fragments arranged by size:
1094 658 531 408 356 279 241 223
129 77
AflIII A'CryG_T
Cuts at: 0 1007 3996
Size: 1007 2989
AhdI GACnn_n'nnGTC
Cuts at: 0 244 3996
Size: 244 3752
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 349 3996
Size: 349 3647
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 823 855 3996
Size: 823 32 3141
Fragments arranged by size:
3141 823 32
AlwNI CAG_nnn'CTG
Cuts at: 0 495 1737 2403 3033 3246 3284 3703 3966
Size: 495 1242 666 630 213 38 419 263
Cuts at: 3966 3996
Size: 30
Fragments arranged by size:
1242 666 630 495 419 263 213 38
30
ApaI G_GGCC'C
Cuts at: 0 2697 2887 3067 3996
Size: 2697 190 180 929
Fragments arranged by size:
2697 929 190 180
ApaLI G'TGCA_C
Cuts at: 0 1011 3996
Size: 1011 2985
AvaI C'yCGr_G
Cuts at: 0 241 1526 2083 2518 2924 3148 3793 3996
Size: 241 1285 557 435 406 224 645 203
Fragments arranged by size:
1285 645 557 435 406 241 224 203
BamHI G'GATC_C
Cuts at: 0 12 700 3996
Size: 12 688 3296
Fragments arranged by size:
3296 688 12
BanI G'GyrC_C
Cuts at: 0 55 672 1069 1085 1590 3011 3706 3996
Size: 55 617 397 16 505 1421 695 290
Fragments arranged by size:
1421 695 617 505 397 290 55 16
BanII G_rGCy'C
Cuts at: 0 650 1198 2697 2887 2950 3067 3490 3724
Size: 650 548 1499 190 63 117 423 234
Cuts at: 3724 3792 3996
Size: 68 204
Fragments arranged by size:
1499 650 548 423 234 204 190 117
68 63
BbeI G_GCGC'C
Cuts at: 0 59 3710 3996
Size: 59 3651 286
Fragments arranged by size:
3651 286 59
BbsI GAAGACnn'nnnn_
Cuts at: 0 317 839 3112 3996
Size: 317 522 2273 884
Fragments arranged by size:
2273 884 522 317
BbvCI CC'TCA_GC
Cuts at: 0 1342 1570 2816 2995 3996
Size: 1342 228 1246 179 1001
Fragments arranged by size:
1342 1246 1001 228 179
BciVI GTATCCnnnnn_n'
Cuts at: 0 2419 3996
Size: 2419 1577
BglI GCCn_nnn'nGGC
Cuts at: 0 926 1531 1975 2359 3996
Size: 926 605 444 384 1637
Fragments arranged by size:
1637 926 605 444 384
BglII A'GATC_T
Cuts at: 0 1958 3996
Size: 1958 2038
BlpI GC'TnA_GC
Cuts at: 0 1019 2557 3996
Size: 1019 1538 1439
Fragments arranged by size:
1538 1439 1019
Bme1580I G_kGCm'C
Cuts at: 0 677 1015 1072 2055 2658 2697 2887 3067
Size: 677 338 57 983 603 39 190 180
Cuts at: 3067 3369 3996
Size: 302 627
Fragments arranged by size:
983 677 627 603 338 302 190 180
57 39
BmgBI CAC'GTC
Cuts at: 0 984 2253 3996
Size: 984 1269 1743
Fragments arranged by size:
1743 1269 984
BmrI ACTGGGnnnn_n'
Cuts at: 0 495 3467 3996
Size: 495 2972 529
Fragments arranged by size:
2972 529 495
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 410 1773 3996
Size: 410 1363 2223
Fragments arranged by size:
2223 1363 410
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 193 1228 1414 1768 2557 2566 3085
Size: 56 137 1035 186 354 789 9 519
Cuts at: 3085 3277 3366 3996
Size: 192 89 630
Fragments arranged by size:
1035 789 630 519 354 192 186 137
89 56 9
Bpu10I CC'TnA_GC
Cuts at: 0 134 1100 1192 1322 1342 1570 2816 2995
Size: 134 966 92 130 20 228 1246 179
Cuts at: 2995 3653 3996
Size: 658 343
Fragments arranged by size:
1246 966 658 343 228 179 134 130
92 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 811 1804 3996
Size: 670 141 993 2192
Fragments arranged by size:
2192 993 670 141
BsaI GGTCTCn'nnnn_
Cuts at: 0 381 567 2080 3996
Size: 381 186 1513 1916
Fragments arranged by size:
1916 1513 381 186
BsaAI yAC'GTr
Cuts at: 0 1008 1065 3996
Size: 1008 57 2931
Fragments arranged by size:
2931 1008 57
BsaHI Gr'CG_yC
Cuts at: 0 56 3679 3707 3996
Size: 56 3623 28 289
Fragments arranged by size:
3623 289 56 28
BsaWI w'CCGG_w
Cuts at: 0 1721 3996
Size: 1721 2275
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 538 568 994 1024 1243 1273 1486 1516
Size: 538 30 426 30 219 30 213 30
Cuts at: 1516 2017 2047 3115 3145 3996
Size: 501 30 1068 30 851
Fragments arranged by size:
1068 851 538 501 426 219 213 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 756 1417 1789 1807 2221 2236 2245 2248
Size: 756 661 372 18 414 15 9 3
Cuts at: 2248 2251 2347 2350 3996
Size: 3 96 3 1646
Fragments arranged by size:
1646 756 661 414 372 96 18 15
9 3 3 3
BseYI C'CCAG_C
Cuts at: 0 20 662 924 1848 1979 1984 2296 2307
Size: 20 642 262 924 131 5 312 11
Cuts at: 2307 2340 2866 3207 3616 3660 3724 3925 3996
Size: 33 526 341 409 44 64 201 71
Fragments arranged by size:
924 642 526 409 341 312 262 201
131 71 64 44 33 20 11 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 946 1372 1657 2174 2599 2683 2743
Size: 345 601 426 285 517 425 84 60
Cuts at: 2743 3376 3996
Size: 633 620
Fragments arranged by size:
633 620 601 517 426 425 345 285
84 60
BsiEI CG_ry'CG
Cuts at: 0 72 3996
Size: 72 3924
BsiHKAI G_wGCw'C
Cuts at: 0 422 650 1007 1015 2950 3023 3323 3792
Size: 422 228 357 8 1935 73 300 469
Cuts at: 3792 3996
Size: 204
Fragments arranged by size:
1935 469 422 357 300 228 204 73
8
BsmI GAATG_Cn'
Cuts at: 0 2529 3996
Size: 2529 1467
BsmBI CGTCTCn'nnnn_
Cuts at: 0 136 974 2417 3523 3996
Size: 136 838 1443 1106 473
Fragments arranged by size:
1443 1106 838 473 136
Bsp1286I G_dGCh'C
Cuts at: 0 422 650 677 1007 1015 1072 1198 2055
Size: 422 228 27 330 8 57 126 857
Cuts at: 2055 2658 2697 2887 2950 3023 3067 3323 3369
Size: 603 39 190 63 73 44 256 46
Cuts at: 3369 3490 3724 3792 3996
Size: 121 234 68 204
Fragments arranged by size:
857 603 422 330 256 234 228 204
190 126 121 73 68 63 57 46
44 39 27 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 368 399 818 1410 1853 1873 2795 3292
Size: 368 31 419 592 443 20 922 497
Cuts at: 3292 3597 3819 3996
Size: 305 222 177
Fragments arranged by size:
922 592 497 443 419 368 305 222
177 31 20
BsrBI CCG'CTC
Cuts at: 0 1104 2139 2715 3996
Size: 1104 1035 576 1281
Fragments arranged by size:
1281 1104 1035 576
BsrDI GCAATG_nn'
Cuts at: 0 122 386 574 797 3996
Size: 122 264 188 223 3199
Fragments arranged by size:
3199 264 223 188 122
BsrFI r'CCGG_y
Cuts at: 0 3376 3598 3996
Size: 3376 222 398
Fragments arranged by size:
3376 398 222
BssHII G'CGCG_C
Cuts at: 0 2626 2685 3996
Size: 2626 59 1311
Fragments arranged by size:
2626 1311 59
BssSI C'ACGA_G
Cuts at: 0 1801 3996
Size: 1801 2195
BstAPI GCAn_nnn'nTGC
Cuts at: 0 672 1794 2403 3018 3996
Size: 672 1122 609 615 978
Fragments arranged by size:
1122 978 672 615 609
BstEII G'GTnAC_C
Cuts at: 0 2919 3186 3996
Size: 2919 267 810
Fragments arranged by size:
2919 810 267
BstXI CCAn_nnnn'nTGG
Cuts at: 0 804 851 2904 3784 3996
Size: 804 47 2053 880 212
Fragments arranged by size:
2053 880 804 212 47
BstYI r'GATC_y
Cuts at: 0 12 584 700 1478 1958 2474 3467 3996
Size: 12 572 116 778 480 516 993 529
Fragments arranged by size:
993 778 572 529 516 480 116 12
BtgI C'CryG_G
Cuts at: 0 158 844 1936 2879 3803 3996
Size: 158 686 1092 943 924 193
Fragments arranged by size:
1092 943 924 686 193 158
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1292 3996
Size: 1292 2704
BtsI GCAGTG_nn'
Cuts at: 0 1013 1548 1694 2810 3996
Size: 1013 535 146 1116 1186
Fragments arranged by size:
1186 1116 1013 535 146
DraIII CAC_nnn'GTG
Cuts at: 0 969 1635 1674 3018 3996
Size: 969 666 39 1344 978
Fragments arranged by size:
1344 978 969 666 39
EaeI y'GGCC_r
Cuts at: 0 155 758 895 1112 1424 2022 2733 3996
Size: 155 603 137 217 312 598 711 1263
Fragments arranged by size:
1263 711 603 598 312 217 155 137
EarI CTCTTCn'nnn_
Cuts at: 0 655 2198 3007 3280 3628 3845 3996
Size: 655 1543 809 273 348 217 151
Fragments arranged by size:
1543 809 655 348 273 217 151
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 805 907 979 3996
Size: 805 102 72 3017
Fragments arranged by size:
3017 805 102 72
EcoICRI GAG'CTC
Cuts at: 0 648 2948 3790 3996
Size: 648 2300 842 206
Fragments arranged by size:
2300 842 648 206
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 193 241 772 901 1228 1257 1414
Size: 56 137 48 531 129 327 29 157
Cuts at: 1414 1536 1759 1768 2167 2557 2566 3085 3261
Size: 122 223 9 399 390 9 519 176
Cuts at: 3261 3277 3366 3919 3996
Size: 16 89 553 77
Fragments arranged by size:
553 531 519 399 390 327 223 176
157 137 129 122 89 77 56 48
29 16 9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 364 556 592 1869 2494 3181 3358 3996
Size: 364 192 36 1277 625 687 177 638
Fragments arranged by size:
1277 687 638 625 364 192 177 36
EcoO109I rG'GnC_Cy
Cuts at: 0 492 537 597 2607 2693 2694 3144 3174
Size: 492 45 60 2010 86 1 450 30
Cuts at: 3174 3281 3657 3797 3996
Size: 107 376 140 199
Fragments arranged by size:
2010 492 450 376 199 140 107 86
60 45 30 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3321 3537 3873 3923 3996
Size: 3321 216 336 50 73
Fragments arranged by size:
3321 336 216 73 50
FspI TGC'GCA
Cuts at: 0 1700 2581 3996
Size: 1700 881 1415
Fragments arranged by size:
1700 1415 881
HaeII r_GCGC'y
Cuts at: 0 59 826 1710 2308 2664 3438 3710 3996
Size: 59 767 884 598 356 774 272 286
Fragments arranged by size:
884 774 767 598 356 286 272 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 230 262 380 412 538 570 971
Size: 29 201 32 118 32 126 32 401
Cuts at: 971 1003 1243 1275 1520 1552 3115 3147 3962
Size: 32 240 32 245 32 1563 32 815
Cuts at: 3962 3994 3996
Size: 32 2
Fragments arranged by size:
1563 815 401 245 240 201 126 118
32 32 32 32 32 32 32 32
29 2
HincII GTy'rAC
Cuts at: 0 1269 3996
Size: 1269 2727
KasI G'GCGC_C
Cuts at: 0 55 3706 3996
Size: 55 3651 290
Fragments arranged by size:
3651 290 55
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3425 3996
Size: 3425 571
MscI TGG'CCA
Cuts at: 0 897 1426 2024 3996
Size: 897 529 598 1972
Fragments arranged by size:
1972 897 598 529
MspA1I CmG'CkG
Cuts at: 0 294 960 1110 2277 2553 2900 3162 3574
Size: 294 666 150 1167 276 347 262 412
Cuts at: 3574 3996
Size: 422
Fragments arranged by size:
1167 666 422 412 347 294 276 262
150
NarI GG'CG_CC
Cuts at: 0 56 3707 3996
Size: 56 3651 289
Fragments arranged by size:
3651 289 56
NruI TCG'CGA
Cuts at: 0 98 3996
Size: 98 3898
NspI r_CATG'y
Cuts at: 0 1320 2877 3996
Size: 1320 1557 1119
Fragments arranged by size:
1557 1320 1119
PasI CC'CwG_GG
Cuts at: 0 1605 3423 3996
Size: 1605 1818 573
Fragments arranged by size:
1818 1605 573
PflMI CCAn_nnn'nTGG
Cuts at: 0 2676 3703 3996
Size: 2676 1027 293
Fragments arranged by size:
2676 1027 293
PfoI T'CCnGG_A
Cuts at: 0 2267 2534 3996
Size: 2267 267 1462
Fragments arranged by size:
2267 1462 267
PmlI CAC'GTG
Cuts at: 0 1008 3996
Size: 1008 2988
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 700 732 3996
Size: 700 32 3264
Fragments arranged by size:
3264 700 32
PpuMI rG'GwC_Cy
Cuts at: 0 597 2607 3144 3174 3281 3657 3797 3996
Size: 597 2010 537 30 107 376 140 199
Fragments arranged by size:
2010 597 537 376 199 140 107 30
PspOMI G'GGCC_C
Cuts at: 0 2693 2883 3063 3996
Size: 2693 190 180 933
Fragments arranged by size:
2693 933 190 180
PstI C_TGCA'G
Cuts at: 0 338 491 1211 1733 1833 2060 2294 2444
Size: 338 153 720 522 100 227 234 150
Cuts at: 2444 3338 3594 3755 3816 3996
Size: 894 256 161 61 180
Fragments arranged by size:
894 720 522 338 256 234 227 180
161 153 150 100 61
PvuII CAG'CTG
Cuts at: 0 294 960 1110 2277 2900 3162 3996
Size: 294 666 150 1167 623 262 834
Fragments arranged by size:
1167 834 666 623 294 262 150
SacI G_AGCT'C
Cuts at: 0 650 2950 3792 3996
Size: 650 2300 842 204
Fragments arranged by size:
2300 842 650 204
SanDI GG'GwC_CC
Cuts at: 0 3797 3996
Size: 3797 199
SapI GCTCTTCn'nnn_
Cuts at: 0 655 3845 3996
Size: 655 3190 151
Fragments arranged by size:
3190 655 151
SbfI CC_TGCA'GG
Cuts at: 0 1211 3816 3996
Size: 1211 2605 180
Fragments arranged by size:
2605 1211 180
SexAI A'CCwGG_T
Cuts at: 0 965 1631 2489 2915 3996
Size: 965 666 858 426 1081
Fragments arranged by size:
1081 965 858 666 426
SfcI C'TryA_G
Cuts at: 0 273 334 487 1207 1729 1829 2056 2290
Size: 273 61 153 720 522 100 227 234
Cuts at: 2290 2440 3334 3355 3590 3751 3812 3996
Size: 150 894 21 235 161 61 184
Fragments arranged by size:
894 720 522 273 235 234 227 184
161 153 150 100 61 61 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1975 2359 3996
Size: 1975 384 1637
Fragments arranged by size:
1975 1637 384
SfoI GGC'GCC
Cuts at: 0 57 3708 3996
Size: 57 3651 288
Fragments arranged by size:
3651 288 57
SgrAI Cr'CCGG_yG
Cuts at: 0 3598 3996
Size: 3598 398
SmaI CCC'GGG
Cuts at: 0 2926 3795 3996
Size: 2926 869 201
Fragments arranged by size:
2926 869 201
SmlI C'TyrA_G
Cuts at: 0 685 826 1819 3996
Size: 685 141 993 2177
Fragments arranged by size:
2177 993 685 141
StuI AGG'CCT
Cuts at: 0 1151 1408 1714 3088 3996
Size: 1151 257 306 1374 908
Fragments arranged by size:
1374 1151 908 306 257
StyI C'CwwG_G
Cuts at: 0 950 1146 1262 3224 3305 3461 3996
Size: 950 196 116 1962 81 156 535
Fragments arranged by size:
1962 950 535 196 156 116 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1840 2299 2668 3316 3496 3996
Size: 1840 459 369 648 180 500
Fragments arranged by size:
1840 648 500 459 369 180
TatI w'GTAC_w
Cuts at: 0 2930 3996
Size: 2930 1066
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 994 1026 1486 1518 1921 1953 2017 2049
Size: 994 32 460 32 403 32 64 32
Cuts at: 2049 3136 3168 3678 3710 3996
Size: 1087 32 510 32 286
Fragments arranged by size:
1087 994 510 460 403 286 64 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3111 3996
Size: 3111 885
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1606 2361 3420 3996
Size: 1606 755 1059 576
Fragments arranged by size:
1606 1059 755 576
XmaI C'CCGG_G
Cuts at: 0 2924 3793 3996
Size: 2924 869 203
Fragments arranged by size:
2924 869 203
XmnI GAAnn'nnTTC
Cuts at: 0 712 3996
Size: 712 3284
Enzymes that do cut:
AarI AclI AcuI AflIII AhdI AlfI AloI AlwNI
ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstEII BstXI BstYI BtgI BtgZI BtsI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I HincII KasI MmeI MscI MspA1I NarI
NruI NspI PasI PflMI PfoI PmlI PpiI PpuMI
PspOMI PstI PvuII SacI SanDI SapI SbfI SexAI
SfcI SfiI SfoI SgrAI SmaI SmlI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AgeI AleI ApoI
AscI AseI AsiSI AvrII BaeI BcgI BclI BfrBI
BmtI BsaBI BsiWI BspEI BspHI BsrGI BstBI BstZ17I
Bsu36I ClaI CspCI DraI DrdI EagI EcoRI EcoRV
FseI FspAI HindIII HpaI KpnI MfeI MluI MslI
NaeI NcoI NdeI NgoMIV NheI NotI NsiI PacI
PciI PmeI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI ScaI SgfI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XhoI ZraI