Restriction map for
pFN21AE2382
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# NO CUT ENZYMES ( 87 enzymes) #
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AatII Acc65I AccI AccIII AcsI
AflII AgeI AocI Aor51HI ApoI
AscI AseI AsnI Asp718I AvrII
BcgI BclI BfrI BlnI BsaBI
BseAI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI KspI
MamI MfeI MluI MroI MseI
MslI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI PvuI RcaI RsrII SacII
SalI ScaI SfuI SnaBI SpeI
SphI SspBI SspI SwaI Tru9I
XbaI XhoI
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# ONE CUT ENZYMES ( 27 enzymes) #
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AclI 3268 AACGTT AA^CGTT
AflIII 1007 ACGTGT A^C[GA][CT]GT
AhdI 239 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1011 GTGCAC G^TGCAC
ApaLI 1011 GTGCAC G^TGCAC
Asp700I 708 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 239 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3108 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1006 CACGTG CAC^GTG
BciVI 2408 GTATCC GTATCC(6/5)
BglII 1958 AGATCT A^GATCT
BsaWI 1721 ACCGGA [AT]^CCGG[AT]
BsiEI 69 CGACCG CG[GA][CT]^CG
BsmI 2523 GAATGC GAATGC(1/-1)
BssSI 1801 CTCGTG CACGAG(-5/-1)
Eam1105I 239 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 1267 GTTGAC GT[CT]^[GA]AC
HindII 1267 GTTGAC GT[CT]^[GA]AC
NruI 96 TCGCGA TCG^CGA
PflFI 3108 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1006 CACGTG CAC^GTG
PmlI 1006 CACGTG CAC^GTG
Psp1406I 3268 AACGTT AA^CGTT
SgrAI 3597 CACCGGCG C[GA]^CCGG[CT]G
Tsp509I 2064 AATT ^AATT
Tth111I 3108 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 708 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
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AfaI 504 GTAC GT^AC
2931 GTAC
AviII 1698 TGCGCA TGC^GCA
2579 TGCGCA
BamHI 12 GGATCC G^GATCC
700 GGATCC
BbeI 55 GGCGCC GGCGC^C
3706 GGCGCC
BlpI 1018 GCTAAGC GC^T[ACGT]AGC
2556 GCTGAGC
Bpu1102I 1018 GCTAAGC GC^T[ACGT]AGC
2556 GCTGAGC
BsaAI 1006 CACGTG [CT]AC^GT[GA]
1063 TACGTG
BsrFI 3376 ACCGGC [GA]^CCGG[CT]
3598 ACCGGC
BssHII 2626 GCGCGC G^CGCGC
2685 GCGCGC
BstEII 2919 GGTGACC G^GT[ACGT]ACC
3186 GGTCACC
BstPI 2919 GGTGACC G^GT[ACGT]ACC
3186 GGTCACC
CelII 1018 GCTAAGC GC^T[ACGT]AGC
2556 GCTGAGC
Cfr10I 3376 ACCGGC [GA]^CCGG[CT]
3598 ACCGGC
EcoO65I 2919 GGTGACC G^GT[ACGT]ACC
3186 GGTCACC
FspI 1698 TGCGCA TGC^GCA
2579 TGCGCA
KasI 55 GGCGCC G^GCGCC
3706 GGCGCC
NarI 55 GGCGCC GG^CGCC
3706 GGCGCC
NspI 1316 ACATGC [GA]CATG^[CT]
2873 ACATGC
PflMI 2670 CCACGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3697 CCAGCCCCTGG
RsaI 504 GTAC GT^AC
2931 GTAC
SapI 648 GCTCTTC GCTCTTC(1/4)
3838 GCTCTTC
SbfI 1206 CCTGCAGG CCTGCA^GG
3811 CCTGCAGG
SfiI 1968 GGCCCTGTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2352 GGCCATGATGGCC
SfoI 55 GGCGCC GGC^GCC
3706 GGCGCC
SmaI 2924 CCCGGG CCC^GGG
3793 CCCGGG
Sse8387I 1206 CCTGCAGG CCTGCA^GG
3811 CCTGCAGG
Van91I 2670 CCACGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3697 CCAGCCCCTGG
XmaCI 2924 CCCGGG C^CCGGG
3793 CCCGGG
XmaI 2924 CCCGGG C^CCGGG
3793 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII SacI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I XcmI XhoII