Restriction map for
pFN21AE4378
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AvrII BamHI BanII BbeI BbrPI
BciVI BclI BfaI BfrI BlnI
BpmI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsmBI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HaeII HgaI Hin1I HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MroI MseI
MunI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI StuI SwaI Tru9I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccI 124 GTCTAC GT^[AC][GT]AC
AccII 521 CGCG CG^CG
AflIII 472 ACGTGT A^C[GA][CT]GT
AlwI 105 GATCC GGATC(4/5)
AlwNI 387 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1095 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 355 GAGCAC G[AT]GC[AT]^C
AspI 265 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 171 CTCGAG C^[CT]CG[GA]G
AviII 349 TGCGCA TGC^GCA
BalI 1031 TGGCCA TGG^CCA
BanI 491 GGTGCC G^G[CT][GA]CC
BbvCI 1111 GCTGAGG CCTCAGC(-5/-2)
BcgI 761 GCATCACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 968 CCGGG CC^[GC]GG
BglI 1071 GCCTAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 479 AGATCT A^GATCT
BlpI 1135 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1135 GCTGAGC GC^T[ACGT]AGC
BsgI 667 CTGCAC GTGCAG(16/14)
BsiHKAI 355 GAGCAC G[AT]GC[AT]^C
BsiWI 798 CGTACG C^GTACG
BsmAI 193 GTCTC GTCTC(1/5)
BsmI 627 GAATGC GAATGC(1/-1)
BsoBI 171 CTCGAG C^[CT]CG[GA]G
BspHI 305 TCATGA T^CATGA
BsrBI 174 GAGCGG CCGCTC(-3/-3)
BstUI 521 CGCG CG^CG
BstXI 225 CCAGCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 479 AGATCT [GA]^GATC[CT]
CelII 1135 GCTGAGC GC^T[ACGT]AGC
DraII 209 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 886 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 209 AGGGCCT [GA]G^G[ACGT]CC[CT]
FspI 349 TGCGCA TGC^GCA
HapII 968 CCGG C^CGG
HincII 598 GTCAAC GT[CT]^[GA]AC
HindII 598 GTCAAC GT[CT]^[GA]AC
HpaII 968 CCGG C^CGG
MflI 479 AGATCT [GA]^GATC[CT]
MluNI 1031 TGGCCA TGG^CCA
MscI 1031 TGGCCA TGG^CCA
MslI 948 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 109 CAGCTG C[AC]G^C[GT]G
MspI 968 CCGG C^CGG
MvnI 521 CGCG CG^CG
NciI 968 CCGGG CC^[GC]GG
NcoI 828 CCATGG C^CATGG
NdeI 372 CATATG CA^TATG
PaeR7I 171 CTCGAG C^TCGAG
PflFI 265 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 109 CAGCTG CAG^CTG
RcaI 305 TCATGA T^CATGA
SbfI 1173 CCTGCAGG CCTGCA^GG
SexAI 611 ACCTGGT A^CC[AT]GGT
Sse8387I 1173 CCTGCAGG CCTGCA^GG
TfiI 936 GATTC G^A[AT]TC
Tth111I 265 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 171 CTCGAG C^TCGAG
XhoII 479 AGATCT [GA]^GATC[CT]
XmnI 1095 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AvaII 117 GGTCC G^G[AT]CC
902 GGACC
BsaAI 700 TACGTG [CT]AC^GT[GA]
796 CACGTA
BseRI 19 GAGGAG GAGGAG(10/8)
619 GAGGAG
BsmFI 287 GGGAC GGGAC(10/14)
901 GGGAC
BspMI 646 ACCTGC ACCTGC(4/8)
1049 ACCTGC
Cac8I 24 GCTGGC GC[ACGT]^[ACGT]GC
1172 GCCTGC
DpnII 105 GATC ^GATC
480 GATC
DsaI 497 CCGTGG C^C[GA][CT]GG
828 CCATGG
EcoNI 612 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
918 CCTCTTCAAGG
EcoT14I 423 CCAAGG C^C[AT][AT]GG
828 CCATGG
FauI 56 CCCGC CCCGC(4/6)
519 CCCGC
MboI 105 GATC ^GATC
480 GATC
NdeII 105 GATC ^GATC
480 GATC
PflMI 108 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
767 CCATCGACTGG
PleI 336 GACTC GAGTC(4/5)
712 GAGTC
PstI 807 CTGCAG CTGCA^G
1174 CTGCAG
Sau3AI 105 GATC ^GATC
480 GATC
SfcI 807 CTGCAG C^T[GA][CT]AG
1174 CTGCAG
StyI 423 CCAAGG C^C[AT][AT]GG
828 CCATGG
Tsp509I 313 AATT ^AATT
1014 AATT
Van91I 108 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
767 CCATCGACTGG
XcmI 881 CCAGGCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1157 CCAGCTCCACCCTGG
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI BbsI BbvI
BmyI BpuAI BsaJI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
CfoI Cfr13I DdeI EaeI EarI
EcoRII Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeII MaeIII MboII MnlI MvaI
MwoI NlaIII NlaIV RsaI Sau96I
ScrFI SfaNI SmlI TaqI TseI
Tsp45I TspRI TthHB8I