(Circular) (Six-base) MAPSORT of: pFN21AE4378.seq Check: 3463 from: 1 to: 5902 pFN21AE4378 5902 bp With 182 enzymes: SgfI * August 16, 2021 15:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3712 278 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2130 3245 1937 Size: 193 1115 4594 Fragments arranged by size: 4594 1115 193 Acc65I G'GTAC_C Cuts at: 3223 3223 Size: 5902 AclI AA'CG_TT Cuts at: 1563 4029 4402 1563 Size: 2466 373 3063 Fragments arranged by size: 3063 2466 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2716 2860 3965 5349 5617 1632 Size: 1084 144 1105 1384 268 1917 Fragments arranged by size: 1917 1384 1105 1084 268 144 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5705 AflIII A'CryG_T Cuts at: 1203 2477 4801 5778 1203 Size: 1274 2324 977 1327 Fragments arranged by size: 2324 1327 1274 977 AgeI A'CCGG_T Cuts at: 4733 4733 Size: 5902 AhdI GACnn_n'nnGTC Cuts at: 4631 4631 Size: 5902 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5902 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5902 AlwNI CAG_nnn'CTG Cuts at: 2397 3645 5217 2397 Size: 1248 1572 3082 Fragments arranged by size: 3082 1572 1248 ApaLI G'TGCA_C Cuts at: 3959 5115 3959 Size: 1156 4746 ApoI r'AATT_y Cuts at: 1493 3211 3437 3589 5698 1493 Size: 1718 226 152 2109 1697 Fragments arranged by size: 2109 1718 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4456 160 Size: 872 3424 1606 Fragments arranged by size: 3424 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 AvaI C'yCGr_G Cuts at: 1870 1943 2176 3227 1870 Size: 73 233 1051 4545 Fragments arranged by size: 4545 1051 233 73 BamHI G'GATC_C Cuts at: 3232 3599 3232 Size: 367 5535 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2496 3223 4678 618 Size: 325 217 627 709 727 1455 1842 Fragments arranged by size: 1842 1455 727 709 627 325 217 BanII G_rGCy'C Cuts at: 1391 3221 1391 Size: 1830 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2269 2394 2480 2654 2683 928 Size: 971 370 125 86 174 29 4147 Fragments arranged by size: 4147 971 370 174 125 86 29 BbvCI CC'TCA_GC Cuts at: 3117 3117 Size: 5902 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2755 2789 4093 4127 1903 Size: 34 818 34 1304 34 3678 Fragments arranged by size: 3678 1304 818 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3794 5004 3794 Size: 1210 4692 BclI T'GATC_A Cuts at: 1548 1548 Size: 5902 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3082 4513 136 Size: 107 122 71 631 605 1410 1431 1525 Fragments arranged by size: 1525 1431 1410 631 605 122 107 71 BglII A'GATC_T Cuts at: 2484 5897 2484 Size: 3413 2489 BlpI GC'TnA_GC Cuts at: 3141 3319 3141 Size: 178 5724 Bme1580I G_kGCm'C Cuts at: 1790 2501 2687 3963 5119 1790 Size: 711 186 1276 1156 2573 Fragments arranged by size: 2573 1276 1156 711 186 BmgBI CAC'GTC Cuts at: 1318 2478 1318 Size: 1160 4742 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2681 3633 4591 Size: 416 53 83 399 24 1264 952 958 Cuts at: 4591 442 Size: 1753 Fragments arranged by size: 1753 1264 958 952 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5902 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4562 1239 Size: 3323 2579 Bpu10I CC'TnA_GC Cuts at: 3117 3117 Size: 5902 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2195 2328 4027 4892 5190 2195 Size: 133 1699 865 298 2907 Fragments arranged by size: 2907 1699 865 298 133 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4565 882 Size: 617 3066 2219 Fragments arranged by size: 3066 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2707 2803 3693 493 Size: 711 1503 96 890 2702 Fragments arranged by size: 2702 1503 890 711 96 BsaBI GATnn'nnATC Cuts at: 5896 5896 Size: 5902 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3709 4091 4726 275 Size: 53 83 186 936 2176 382 635 1451 Fragments arranged by size: 2176 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4335 4733 5007 5154 5565 4335 Size: 398 274 147 411 4672 Fragments arranged by size: 4672 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5677 Fragments arranged by size: 5677 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 2639 1186 Size: 853 600 4449 Fragments arranged by size: 4449 853 600 BseYI C'CCAG_C Cuts at: 2229 2281 5105 5601 2229 Size: 52 2824 496 2530 Fragments arranged by size: 2824 2530 496 52 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2657 1163 Size: 573 921 4408 Fragments arranged by size: 4408 921 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3352 4113 5141 1540 Size: 275 186 1351 761 1028 2301 Fragments arranged by size: 2301 1351 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2364 3963 4048 5119 2364 Size: 1599 85 1071 3147 Fragments arranged by size: 3147 1599 1071 85 BsiWI C'GTAC_G Cuts at: 2803 2803 Size: 5902 BsmI GAATG_Cn' Cuts at: 2638 3413 3506 2638 Size: 775 93 5034 Fragments arranged by size: 5034 775 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2364 2501 2687 3221 3963 4048 5119 Size: 399 574 137 186 534 742 85 1071 Cuts at: 5119 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 574 534 399 186 137 85 BspEI T'CCGG_A Cuts at: 5565 5565 Size: 5902 BspHI T'CATG_A Cuts at: 2310 3791 5521 2310 Size: 1481 1730 2691 Fragments arranged by size: 2691 1730 1481 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2660 3063 3257 844 Size: 1816 403 194 3489 Fragments arranged by size: 3489 1816 403 194 BsrBI CCG'CTC Cuts at: 1394 1823 2181 3789 1394 Size: 429 358 1608 3507 Fragments arranged by size: 3507 1608 429 358 BsrDI GCAATG_nn' Cuts at: 65 1248 4397 4571 65 Size: 1183 3149 174 1396 Fragments arranged by size: 3149 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4546 4733 1700 Size: 110 2736 187 2869 Fragments arranged by size: 2869 2736 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5902 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5902 BssSI C'ACGA_G Cuts at: 3962 4974 3962 Size: 1012 4890 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5902 BstBI TT'CG_AA Cuts at: 4706 4706 Size: 5902 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5902 BstXI CCAn_nnnn'nTGG Cuts at: 2237 2237 Size: 5902 BstYI r'GATC_y Cuts at: 1972 2484 3232 3599 3984 4001 5453 5539 5551 Size: 512 748 367 385 17 1452 86 12 Cuts at: 5551 5897 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 748 512 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2502 2833 513 Size: 552 939 498 331 3582 Fragments arranged by size: 3582 939 552 498 331 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5163 Fragments arranged by size: 5163 683 56 BtsI GCAGTG_nn' Cuts at: 2200 2809 3418 4212 4232 2200 Size: 609 609 794 20 3870 Fragments arranged by size: 3870 794 609 609 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5902 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5867 DraI TTT'AAA Cuts at: 3206 3206 Size: 5902 DraIII CAC_nnn'GTG Cuts at: 2896 2896 Size: 5902 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4909 809 Size: 507 3593 1802 Fragments arranged by size: 3593 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2220 3036 Size: 54 1425 50 275 18 201 189 816 Cuts at: 3036 3349 4238 5867 8 Size: 313 889 1629 43 Fragments arranged by size: 1629 1425 889 816 313 275 201 189 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3349 1537 Size: 275 1537 4090 Fragments arranged by size: 4090 1537 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2048 2841 2930 3832 1353 Size: 695 793 89 902 3423 Fragments arranged by size: 3423 902 793 695 89 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4489 4863 5009 387 Size: 1064 138 2900 374 146 1280 Fragments arranged by size: 2900 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2716 2860 3965 4562 5349 5617 1239 Size: 393 1084 144 1105 597 787 268 1524 Fragments arranged by size: 1524 1105 1084 787 597 393 268 144 EcoNI CCTnn'n_nnAGG Cuts at: 2621 2927 2621 Size: 306 5596 EcoO109I rG'GnC_Cy Cuts at: 2215 2215 Size: 5902 EcoRI G'AATT_C Cuts at: 3211 3589 3211 Size: 378 5524 FspI TGC'GCA Cuts at: 2356 4408 2356 Size: 2052 3850 HaeII r_GCGC'y Cuts at: 1678 5049 1678 Size: 3371 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4543 4575 4617 4649 1373 Size: 32 135 32 2971 32 42 32 2626 Fragments arranged by size: 2971 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2605 3246 3492 669 Size: 988 281 667 641 246 3079 Fragments arranged by size: 3079 988 667 641 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5902 HpaI GTT'AAC Cuts at: 3492 3492 Size: 5902 KpnI G_GTAC'C Cuts at: 3227 3227 Size: 5902 MfeI C'AATT_G Cuts at: 3501 3501 Size: 5902 MluI A'CGCG_T Cuts at: 5778 5778 Size: 5902 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2128 5016 5200 1305 Size: 823 2888 184 2007 Fragments arranged by size: 2888 2007 823 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3038 10 Size: 54 1425 343 1206 2874 Fragments arranged by size: 2874 1425 1206 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2957 3860 4219 4378 518 Size: 648 1791 903 359 159 2042 Fragments arranged by size: 2042 1791 903 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2116 3318 3995 5143 5388 1606 Size: 510 1202 677 1148 245 2120 Fragments arranged by size: 2120 1202 1148 677 510 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2833 513 Size: 552 939 829 3582 Fragments arranged by size: 3582 939 829 552 NdeI CA'TA_TG Cuts at: 387 2378 387 Size: 1991 3911 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5902 NotI GC'GGCC_GC Cuts at: 3349 3349 Size: 5902 NspI r_CATG'y Cuts at: 1132 1229 3260 4805 1132 Size: 97 2031 1545 2229 Fragments arranged by size: 2229 2031 1545 97 PasI CC'CwG_GG Cuts at: 1348 2287 2405 3172 1348 Size: 939 118 767 4078 Fragments arranged by size: 4078 939 767 118 PciI A'CATG_T Cuts at: 4801 4801 Size: 5902 PflMI CCAn_nnn'nTGG Cuts at: 1108 2119 2778 1108 Size: 1011 659 4232 Fragments arranged by size: 4232 1011 659 PfoI T'CCnGG_A Cuts at: 2019 2019 Size: 5902 PmeI GTTT'AAAC Cuts at: 3206 3206 Size: 5902 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3971 4003 5484 5516 3971 Size: 32 1481 32 4357 Fragments arranged by size: 4357 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5902 PsiI TTA'TAA Cuts at: 3472 3472 Size: 5902 PspXI vC'TCGA_Gb Cuts at: 2176 2176 Size: 5902 PstI C_TGCA'G Cuts at: 830 2816 3183 3254 830 Size: 1986 367 71 3478 Fragments arranged by size: 3478 1986 367 71 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5902 PvuII CAG'CTG Cuts at: 2116 2116 Size: 5902 SalI G'TCGA_C Cuts at: 1936 3244 1936 Size: 1308 4594 SbfI CC_TGCA'GG Cuts at: 3183 3254 3183 Size: 71 5831 ScaI AGT'ACT Cuts at: 4150 5862 4150 Size: 1712 4190 SexAI A'CCwGG_T Cuts at: 2616 2616 Size: 5902 SfcI C'TryA_G Cuts at: 826 1045 2812 3179 3250 4385 5066 5257 826 Size: 219 1767 367 71 1135 681 191 1471 Fragments arranged by size: 1767 1471 1135 681 367 219 191 71 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5902 SmaI CCC'GGG Cuts at: 1872 3229 1872 Size: 1357 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2176 2210 2343 4006 4907 5169 Size: 197 926 233 34 133 1663 901 262 Cuts at: 5169 820 Size: 1553 Fragments arranged by size: 1663 1553 926 901 262 233 197 133 34 SnaBI TAC'GTA Cuts at: 493 3693 493 Size: 3200 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5902 SphI G_CATG'C Cuts at: 1132 3260 1132 Size: 2128 3774 SspI AAT'ATT Cuts at: 5 52 3826 5 Size: 47 3774 2081 Fragments arranged by size: 3774 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5902 StyI C'CwwG_G Cuts at: 513 1065 2004 2428 2833 3341 513 Size: 552 939 424 405 508 3074 Fragments arranged by size: 3074 939 552 508 424 405 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2134 2379 3929 3946 4099 4284 4729 Size: 1255 554 245 1550 17 153 185 445 Cuts at: 4729 325 Size: 1498 Fragments arranged by size: 1550 1498 1255 554 445 245 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4148 5860 96 Size: 274 80 33 51 1443 2171 1712 138 Fragments arranged by size: 2171 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2001 2033 5862 5894 2001 Size: 32 3829 32 2009 Fragments arranged by size: 3829 2009 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2273 1316 Size: 957 4945 XbaI T'CTAG_A Cuts at: 3238 3238 Size: 5902 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2893 3169 2893 Size: 276 5626 XhoI C'TCGA_G Cuts at: 1943 2176 1943 Size: 233 5669 XmaI C'CCGG_G Cuts at: 1870 3227 1870 Size: 1357 4545 XmnI GAAnn'nnTTC Cuts at: 3104 4031 3104 Size: 927 4975 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3710 276 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI