Restriction map for
pFN21AE4378
(Linear) (Six-base) MAPSORT of: KIEE4378.seq Check: 317 from: 1 to: 1197
KIEE4378.orf
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AccI GT'mk_AC
Cuts at: 0 125 1197
Size: 125 1072
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 711 855 1197
Size: 711 144 342
Fragments arranged by size:
711 342 144
AflIII A'CryG_T
Cuts at: 0 472 1197
Size: 472 725
AlwNI CAG_nnn'CTG
Cuts at: 0 392 1197
Size: 392 805
AvaI C'yCGr_G
Cuts at: 0 171 1197
Size: 171 1026
BanI G'GyrC_C
Cuts at: 0 491 1197
Size: 491 706
BbsI GAAGACnn'nnnn_
Cuts at: 0 264 389 475 649 678 1197
Size: 264 125 86 174 29 519
Fragments arranged by size:
519 264 174 125 86 29
BbvCI CC'TCA_GC
Cuts at: 0 1112 1197
Size: 1112 85
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 750 784 1197
Size: 750 34 413
Fragments arranged by size:
750 413 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1077 1197
Size: 1077 120
BglII A'GATC_T
Cuts at: 0 479 1197
Size: 479 718
BlpI GC'TnA_GC
Cuts at: 0 1136 1197
Size: 1136 61
Bme1580I G_kGCm'C
Cuts at: 0 496 682 1197
Size: 496 186 515
Fragments arranged by size:
515 496 186
BmgBI CAC'GTC
Cuts at: 0 473 1197
Size: 473 724
BmrI ACTGGGnnnn_n'
Cuts at: 0 676 1197
Size: 676 521
Bpu10I CC'TnA_GC
Cuts at: 0 1112 1197
Size: 1112 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 323 1197
Size: 190 133 874
Fragments arranged by size:
874 190 133
BsaAI yAC'GTr
Cuts at: 0 702 798 1197
Size: 702 96 399
Fragments arranged by size:
702 399 96
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 634 1197
Size: 34 600 563
Fragments arranged by size:
600 563 34
BseYI C'CCAG_C
Cuts at: 0 224 276 1197
Size: 224 52 921
Fragments arranged by size:
921 224 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 1197
Size: 652 545
BsiHKAI G_wGCw'C
Cuts at: 0 359 1197
Size: 359 838
BsiWI C'GTAC_G
Cuts at: 0 798 1197
Size: 798 399
BsmI GAATG_Cn'
Cuts at: 0 633 1197
Size: 633 564
Bsp1286I G_dGCh'C
Cuts at: 0 359 496 682 1197
Size: 359 137 186 515
Fragments arranged by size:
515 359 186 137
BspHI T'CATG_A
Cuts at: 0 305 1197
Size: 305 892
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 655 1058 1197
Size: 655 403 139
Fragments arranged by size:
655 403 139
BsrBI CCG'CTC
Cuts at: 0 176 1197
Size: 176 1021
BstXI CCAn_nnnn'nTGG
Cuts at: 0 232 1197
Size: 232 965
BstYI r'GATC_y
Cuts at: 0 479 1197
Size: 479 718
BtgI C'CryG_G
Cuts at: 0 497 828 1197
Size: 497 331 369
Fragments arranged by size:
497 369 331
BtsI GCAGTG_nn'
Cuts at: 0 195 804 1197
Size: 195 609 393
Fragments arranged by size:
609 393 195
DraIII CAC_nnn'GTG
Cuts at: 0 891 1197
Size: 891 306
EaeI y'GGCC_r
Cuts at: 0 26 215 1031 1197
Size: 26 189 816 166
Fragments arranged by size:
816 189 166 26
EarI CTCTTCn'nnn_
Cuts at: 0 43 836 925 1197
Size: 43 793 89 272
Fragments arranged by size:
793 272 89 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 711 855 1197
Size: 711 144 342
Fragments arranged by size:
711 342 144
EcoNI CCTnn'n_nnAGG
Cuts at: 0 616 922 1197
Size: 616 306 275
Fragments arranged by size:
616 306 275
EcoO109I rG'GnC_Cy
Cuts at: 0 210 1197
Size: 210 987
FspI TGC'GCA
Cuts at: 0 351 1197
Size: 351 846
HincII GTy'rAC
Cuts at: 0 600 1197
Size: 600 597
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 1197
Size: 123 1074
MscI TGG'CCA
Cuts at: 0 1033 1197
Size: 1033 164
MslI CAynn'nnrTG
Cuts at: 0 952 1197
Size: 952 245
MspA1I CmG'CkG
Cuts at: 0 111 1197
Size: 111 1086
NcoI C'CATG_G
Cuts at: 0 828 1197
Size: 828 369
NdeI CA'TA_TG
Cuts at: 0 373 1197
Size: 373 824
PasI CC'CwG_GG
Cuts at: 0 282 400 1167 1197
Size: 282 118 767 30
Fragments arranged by size:
767 282 118 30
PflMI CCAn_nnn'nTGG
Cuts at: 0 114 773 1197
Size: 114 659 424
Fragments arranged by size:
659 424 114
PfoI T'CCnGG_A
Cuts at: 0 14 1197
Size: 14 1183
PspXI vC'TCGA_Gb
Cuts at: 0 171 1197
Size: 171 1026
PstI C_TGCA'G
Cuts at: 0 811 1178 1197
Size: 811 367 19
Fragments arranged by size:
811 367 19
PvuII CAG'CTG
Cuts at: 0 111 1197
Size: 111 1086
SbfI CC_TGCA'GG
Cuts at: 0 1178 1197
Size: 1178 19
SexAI A'CCwGG_T
Cuts at: 0 611 1197
Size: 611 586
SfcI C'TryA_G
Cuts at: 0 807 1174 1197
Size: 807 367 23
Fragments arranged by size:
807 367 23
SmlI C'TyrA_G
Cuts at: 0 171 205 338 1197
Size: 171 34 133 859
Fragments arranged by size:
859 171 133 34
StyI C'CwwG_G
Cuts at: 0 423 828 1197
Size: 423 405 369
Fragments arranged by size:
423 405 369
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 129 374 1197
Size: 129 245 823
Fragments arranged by size:
823 245 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 28 1197
Size: 28 1169
Tth111I GACn'n_nGTC
Cuts at: 0 268 1197
Size: 268 929
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 888 1164 1197
Size: 888 276 33
Fragments arranged by size:
888 276 33
XhoI C'TCGA_G
Cuts at: 0 171 1197
Size: 171 1026
XmnI GAAnn'nnTTC
Cuts at: 0 1099 1197
Size: 1099 98
Enzymes that do cut:
AccI AcuI AflIII AlwNI AvaI BanI BbsI BbvCI
BcgI BglI BglII BlpI Bme1580I BmgBI BmrI Bpu10I
BpuEI BsaAI BseRI BseYI BsgI BsiHKAI BsiWI BsmI
Bsp1286I BspHI BspMI BsrBI BstXI BstYI BtgI BtsI
DraIII EaeI EarI Eco57MI EcoNI EcoO109I FspI HincII
MmeI MscI MslI MspA1I NcoI NdeI PasI PflMI
PfoI PspXI PstI PvuII SbfI SexAI SfcI SmlI
StyI TaqII TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbeI BciVI BclI
BfrBI BmtI BplI BpmI BsaI BsaBI BsaHI BsaWI
BsaXI BsiEI BsmBI BspEI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EagI EciI EcoICRI EcoRI EcoRV
FalI FseI FspAI HaeII Hin4I HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI XbaI XmaI ZraI