################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DraIII EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MspA1I MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TspRI Tth111I Van91I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccII 329 CGCG CG^CG AcsI 50 AAATTC [GA]^AATT[CT] AluI 59 AGCT AG^CT AlwNI 334 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 296 AGCGCT AGC^GCT ApoI 50 AAATTC [GA]^AATT[CT] Asp700I 129 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 12 CCCGGG C^[CT]CG[GA]G AvaII 117 GGACC G^G[AT]CC BbsI 142 GAAGAC GAAGAC(2/6) BpuAI 142 GAAGAC GAAGAC(2/6) BseRI 304 GAGGAG GAGGAG(10/8) BsgI 352 GTGCAG GTGCAG(16/14) BsiYI 278 CCCGCAAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 278 CCCGCAAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 221 GAGAC GTCTC(1/5) BsmFI 248 GGGAC GGGAC(10/14) BsoBI 12 CCCGGG C^[CT]CG[GA]G BspMI 234 GCAGGT ACCTGC(4/8) BsrFI 21 GCCGGT [GA]^CCGG[CT] BstEII 24 GGTCACC G^GT[ACGT]ACC BstF5I 9 CATCC GGATG(2/0) BstPI 24 GGTCACC G^GT[ACGT]ACC BstUI 329 CGCG CG^CG CfoI 297 GCGC GCG^C Cfr10I 21 GCCGGT [GA]^CCGG[CT] DpnII 228 GATC ^GATC DrdI 327 GACGCGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 296 AGCGCT AGC^GCT EcoO65I 24 GGTCACC G^GT[ACGT]ACC EcoT14I 257 CCATGG C^C[AT][AT]GG FauI 278 CCCGC CCCGC(4/6) FokI 9 CATCC GGATG(9/13) HaeII 296 AGCGCT [GA]GCGC^[CT] HhaI 297 GCGC GCG^C HinP1I 297 GCGC G^CGC MboI 228 GATC ^GATC MboII 142 GAAGA GAAGA(8/7) MseI 79 TTAA T^TAA MvnI 329 CGCG CG^CG NcoI 257 CCATGG C^CATGG NdeII 228 GATC ^GATC NlaIV 117 GGACCC GG[ACGT]^[ACGT]CC PpuMI 116 AGGACCC [GA]G^G[AT]CC[CT] PshAI 255 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Sau3AI 228 GATC ^GATC SmaI 12 CCCGGG CCC^GGG StyI 257 CCATGG C^C[AT][AT]GG TaqI 110 TCGA T^CGA Tru9I 79 TTAA T^TAA Tsp509I 51 AATT ^AATT TthHB8I 110 TCGA T^CGA XmaCI 12 CCCGGG C^CCGGG XmaI 12 CCCGGG C^CCGGG XmnI 129 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BcnI 12 CCCGG CC^[GC]GG 13 CCGGG BstAPI 333 GCAGTCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 357 GCAGTCCATGC DraII 116 AGGACCC [GA]G^G[ACGT]CC[CT] 189 AGGCCCT DsaI 32 CCGTGG C^C[GA][CT]GG 257 CCATGG EcoO109I 116 AGGACCC [GA]G^G[ACGT]CC[CT] 189 AGGCCCT HapII 13 CCGG C^CGG 22 CCGG HinfI 107 GAATC G^A[ACGT]TC 129 GAATC HpaII 13 CCGG C^CGG 22 CCGG MspI 13 CCGG C^CGG 22 CCGG NciI 12 CCCGG CC^[GC]GG 13 CCGGG PstI 211 CTGCAG CTGCA^G 340 CTGCAG SfcI 211 CTGCAG C^T[GA][CT]AG 340 CTGCAG TfiI 107 GAATC G^A[AT]TC 129 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AciI BbvI BsaJI BssKI BstNI Cac8I Cfr13I EcoRII Fnu4HI HaeIII HgaI HphI ItaI MaeIII MnlI MslI MvaI MwoI NlaIII Sau96I ScrFI TseI Tsp45I