################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AviII AvrII BamHI BbeI BbvCI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaWI BseAI BsiEI BsiWI BsmFI BsmI BspDI BspEI BspLU11I BspMI BsrBI BsrDI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraII DraIII DrdI DsaI EagI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HincII HindII HpaI KasI KpnI KspI MaeI MfeI MluI MroI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmeI PpuMI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StyI SwaI TfiI Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AfaI 1068 GTAC GT^AC AhdI 1010 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 1103 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 430 CCTCAGG CC^T[ACGT]AGG AspEI 1010 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1036 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 413 GGTCC G^G[AT]CC BalI 687 TGGCCA TGG^CCA BanII 211 GAGCTC G[GA]GC[CT]^C BbrPI 780 CACGTG CAC^GTG BcgI 1151 CGAAGGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 335 GGATAC GTATCC(6/5) BsaAI 780 CACGTG [CT]AC^GT[GA] BsaBI 651 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 1117 CGTCTC CGTCTC(1/5) Bsp1407I 1067 TGTACA T^GTACA BspHI 440 TCATGA T^CATGA BsrFI 325 ACCGGC [GA]^CCGG[CT] BsrGI 1067 TGTACA T^GTACA BssHII 1096 GCGCGC G^CGCGC BstF5I 1050 CATCC GGATG(2/0) BstYI 395 GGATCT [GA]^GATC[CT] Bsu36I 430 CCTCAGG CC^T[ACGT]AGG Cfr10I 325 ACCGGC [GA]^CCGG[CT] Eam1105I 1010 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 430 CCTCAGG CC^T[ACGT]AGG FokI 1050 CATCC GGATG(9/13) HaeII 361 AGCGCC [GA]GCGC^[CT] HindIII 1140 AAGCTT A^AGCTT MamI 651 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 395 GGATCT [GA]^GATC[CT] MluNI 687 TGGCCA TGG^CCA MscI 687 TGGCCA TGG^CCA MseI 148 TTAA T^TAA PflFI 1036 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 780 CACGTG CAC^GTG PmlI 780 CACGTG CAC^GTG PshAI 117 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 440 TCATGA T^CATGA RsaI 1068 GTAC GT^AC SacI 211 GAGCTC GAGCT^C SapI 735 GCTCTTC GCTCTTC(1/4) SexAI 380 ACCAGGT A^CC[AT]GGT SfaNI 371 GCATC GCATC(5/9) SmlI 208 CTTGAG C^T[CT][GA]AG SspBI 1067 TGTACA T^GTACA SspI 196 AATATT AAT^ATT Tru9I 148 TTAA T^TAA Tth111I 1036 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 710 CCATCGGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 395 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AatII 613 GACGTC GACGT^C 1036 GACGTC AcyI 613 GACGTC G[GA]^CG[CT]C 1036 GACGTC AlwI 351 GATCC GGATC(4/5) 395 GGATC AvaI 330 CCCGAG C^[CT]CG[GA]G 1012 CTCGGG BcnI 920 CCGGG CC^[GC]GG 1081 CCGGG BsaHI 613 GACGTC G[GA]^CG[CT]C 1036 GACGTC BsaI 992 GAGACC GGTCTC(1/5) 1077 GAGACC BsgI 628 CTGCAC GTGCAG(16/14) 661 GTGCAG BsoBI 330 CCCGAG C^[CT]CG[GA]G 1012 CTCGGG BstXI 381 CCAGGTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 752 CCATGATCGTGG Cfr13I 328 GGCCC G^G[ACGT]CC 413 GGTCC EaeI 139 CGGCCA [CT]^GGCC[GA] 687 TGGCCA EarI 736 CTCTTC CTCTTC(1/4) 796 CTCTTC HgaI 978 GACGC GACGC(5/10) 68 GCGTC Hin1I 613 GACGTC G[GA]^CG[CT]C 1036 GACGTC HinfI 61 GAGTC G^A[ACGT]TC 1010 GACTC Ksp632I 736 CTCTTC CTCTTC(1/4) 796 CTCTTC MslI 654 CATCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 753 CATGATCGTG NciI 920 CCGGG CC^[GC]GG 1081 CCGGG PleI 1010 GACTC GAGTC(4/5) 61 GAGTC Sau96I 328 GGCCC G^G[ACGT]CC 413 GGTCC StuI 851 AGGCCT AGG^CCT 1154 AGGCCT Tsp509I 10 AATT ^AATT 445 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AccII AciI AluI AspHI BanI BbsI BbvI BclI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI BstUI Cac8I CfoI DdeI DpnII EcoRII FbaI Fnu4HI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PstI Sau3AI ScrFI SfcI TaqI TseI Tsp45I TspRI TthHB8I